Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.


:Interleukin-10 (IL-10) suppresses the maturation and cytokine production of dendritic cells (DCs), key regulators of adaptive immunity, and prevents the activation and polarization of naïve T cells towards protective gamma interferon-producing effectors. We hypothesized that human cytomegalovirus (HCMV) utilizes its viral IL-10 homolog (cmvIL-10) to attenuate DC functionality, thereby subverting the efficient induction of antiviral immune responses. RNA and protein analyses demonstrated that the cmvIL-10 gene was expressed with late gene kinetics. Treatment of immature DCs (iDCs) with supernatant from HCMV-infected cultures inhibited both the lipopolysaccharide-induced DC maturation and proinflammatory cytokine production. These inhibitory effects were specifically mediated through the IL-10 receptor and were not observed when DCs were treated with supernatant of cells infected with a cmvIL-10-knockout mutant. Incubation of iDCs with recombinant cmvIL-10 recapitulated the inhibition of maturation. Furthermore, cmvIL-10 had pronounced long-term effects on those DCs that could overcome this inhibition of maturation. It enhanced the migration of mature DCs (mDCs) towards the lymph node homing chemokine but greatly reduced their cytokine production. The inability of mDCs to secrete IL-12 was maintained, even when they were restimulated by the activated T-cell signal CD40 ligand in the absence of cmvIL-10. Importantly, cmvIL-10 potentiates these anti-inflammatory effects, at least partially, by inducing endogenous cellular IL-10 expression in DCs. Collectively, we show that cmvIL-10 causes long-term functional alterations at all stages of DC activation.


J Virol


Journal of virology


Chang WL,Baumgarth N,Yu D,Barry PA




Has Abstract


2004-08-01 00:00:00














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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Grayfer L,De Jesús Andino F,Robert J

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  • Infective substructures of measles virus from acutely and persistently infected cells.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rozenblatt S,Koch T,Pinhasi O,Bratosin S

    更新日期:1979-10-01 00:00:00

  • Molecular analyses of a five-amino-acid cytotoxic T-lymphocyte (CTL) epitope: an immunodominant region which induces nonreciprocal CTL cross-reactivity.

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    authors: Whitton JL,Tishon A,Lewicki H,Gebhard J,Cook T,Salvato M,Joly E,Oldstone MB

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  • Herpes Simplex Virus 1 Replication, Ocular Disease, and Reactivations from Latency Are Restricted Unilaterally after Inoculation of Virus into the Lip.

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    journal_title:Journal of virology

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    authors: Poccardi N,Rousseau A,Haigh O,Takissian J,Naas T,Deback C,Trouillaud L,Issa M,Roubille S,Juillard F,Efstathiou S,Lomonte P,Labetoulle M

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  • Mapping a neutralizing epitope onto the capsid of adeno-associated virus serotype 8.

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    authors: Gurda BL,Raupp C,Popa-Wagner R,Naumer M,Olson NH,Ng R,McKenna R,Baker TS,Kleinschmidt JA,Agbandje-McKenna M

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  • A 1.6-kilobase-pair fragment in the genome of the ts1 mutant of Moloney murine leukemia virus TB that is associated with temperature sensitivity, nonprocessing of Pr80env, and paralytogenesis.

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    authors: Abendroth A,Morrow G,Cunningham AL,Slobedman B

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    authors: Mindich L,Bamford D,McGraw T,Mackenzie G

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    pub_type: 杂志文章


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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Biegalke BJ

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kiyono T,Hiraiwa A,Ishii S,Takahashi T,Ishibashi M

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    pub_type: 杂志文章


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