Abstract:
:Human adenoviruses typically cause mild infections in the upper or lower respiratory tract, gastrointestinal tract, or ocular epithelium. However, adenoviruses may be life-threatening in patients with impaired immunity and some serotypes cause epidemic outbreaks. Attachment to host cell receptors activates cell signaling and virus uptake by endocytosis. At present, it is unclear how vital cellular homeostatic mechanisms affect these early steps in the adenovirus life cycle. Autophagy is a lysosomal degradation pathway for recycling intracellular components that is upregulated during periods of cell stress. Autophagic cargo is sequestered in double-membrane structures called autophagosomes that fuse with endosomes to form amphisomes which then deliver their content to lysosomes. Autophagy is an important adaptive response in airway epithelial cells targeted by many common adenovirus serotypes. Using two established tissue culture models, we demonstrate here that adaptive autophagy enhances expression of the early region 1 adenovirus protein, induction of mitogen-activated protein kinase signaling, and production of new viral progeny in airway epithelial cells infected with adenovirus type 2. We have also discovered that adenovirus infections are tightly regulated by endosome maturation, a process characterized by abrupt exchange of Rab5 and Rab7 GTPases, associated with early and late endosomes, respectively. Moreover, endosome maturation appears to control a pool of early endosomes capable of fusing with autophagosomes which enhance adenovirus infection. Many viruses have evolved mechanisms to induce autophagy in order to aid their own replication. Our studies reveal a novel role for host cell autophagy that could have a significant impact on the outcome of respiratory infections.
journal_name
J Viroljournal_title
Journal of virologyauthors
Zeng X,Carlin CRdoi
10.1128/JVI.02014-12subject
Has Abstractpub_date
2013-02-01 00:00:00pages
2307-19issue
4eissn
0022-538Xissn
1098-5514pii
JVI.02014-12journal_volume
87pub_type
杂志文章abstract::The region of the UL component of the herpes simplex virus type 1 genome between nucleotides 17,793 and 25,150 includes three open reading frames that code for the protein products of the UL8, UL9, and UL10 genes (D.J. McGeogh, M.A. Dalrymple, A.J. Davison, A. Dolan, M.C. Frame, D. McNab, L.J. Perry, J.E. Scott, and P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4251-4261.1994
更新日期:1994-07-01 00:00:00
abstract:UNLABELLED:The encephalitic response to viral infection requires local chemokine production and the ensuing recruitment of immune and inflammatory leukocytes. Accordingly, chemokine receptors present themselves as plausible therapeutic targets for drugs aimed at limiting encephalitic responses. However, it remains uncl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03421-13
更新日期:2014-09-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent for KS tumors, multicentric Castleman's disease, and primary effusion lymphomas. Like other herpesvirus capsids, the KSHV capsid is an icosahedral structure composed of six proteins. The capsid shell is made up of the major capsid protein, two tripl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00423-08
更新日期:2008-07-01 00:00:00
abstract::Bacterial lipopolysaccharide (LPS) induces interferon (IFN) secretion and an antiviral state in murine peritoneal macrophages (PM). These cells secrete predominantly IFN-beta, as shown by neutralization assays with monoclonal antibodies. Secretion of IFN-beta is also induced in PM by IFN-gamma. LPS and IFN-gamma syner...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2785-2789.1989
更新日期:1989-06-01 00:00:00
abstract::Vaccinia virus has a wide host range and infects mammalian cells of many different species. This suggests that the cell surface receptors for vaccinia virus are ubiquitously expressed and highly conserved. Alternatively, different receptors are used for vaccinia virus infection of different cell types. Here we report ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1577-1585.1998
更新日期:1998-02-01 00:00:00
abstract::Nidoviruses (arteriviruses, coronaviruses, and roniviruses) are a phylogenetically compact but diverse group of positive-strand RNA viruses that includes important human and animal pathogens. Nidovirus RNA synthesis is mediated by a cytoplasmic membrane-associated replication/transcription complex that includes up to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00261-09
更新日期:2009-06-01 00:00:00
abstract:UNLABELLED:Antibodies against the fusion (F) protein of respiratory syncytial virus (RSV) play an important role in the protective immune response to this important respiratory virus. Little is known, however, about antibody levels against multiple F-specific epitopes induced by infection or after vaccination against R...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00235-16
更新日期:2016-06-10 00:00:00
abstract::Klebsiella pneumoniae is a member of the family Enterobacteriaceae, opportunistic pathogens that are among the eight most prevalent infectious agents in hospitals. The emergence of multidrug-resistant strains of K. pneumoniae has became a public health problem globally. To develop an effective antimicrobial agent, we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02435-12
更新日期:2012-12-01 00:00:00
abstract::Chick embryo cells became more sensitive to the action of interferon the longer they remained in culture. This phenomenon was found even before confluency had been reached. The relative insensitivity of newly seeded cells was not due to a loss of receptors. Cells synthesizing deoxyribonucleic acid (DNA) at a high rate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.1.7-11.1969
更新日期:1969-07-01 00:00:00
abstract::A cell model of primary macrophages isolated from the peritoneal cavity of flavivirus-susceptible and congenic resistant mice has been used to study the extent and kinetics of antiviral effects against West Nile virus upon priming with alpha/beta interferon (IFN-alpha/beta) or poly(I-C) (pIC). The pattern of flaviviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1753-1764.2005
更新日期:2005-02-01 00:00:00
abstract::Intact, purified particles of the nodaviruses flock house virus and nodamura virus that were either transfected into cells that were resistant to infection or introduced into in vitro translation systems directed the synthesis of viral proteins. We infer that direct interaction of these nodavirus particles with cytopl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7974-7977.1997
更新日期:1997-10-01 00:00:00
abstract::The papillomavirus (PV) E2 protein is a critical regulator of viral transcription and genome replication. We previously reported that tyrosine (Y) 138 of HPV-31 E2 is phosphorylated by the fibroblast growth factor receptor 3 (FGFR3) kinase. In this study, we generated quasiviruses containing G418-selectable HPV-31 gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00488-20
更新日期:2020-07-01 00:00:00
abstract:UNLABELLED:Gammaherpesviruses establish persistent, systemic infections and cause cancers. Murid herpesvirus 4 (MuHV-4) provides a unique window into the early events of host colonization. It spreads via lymph nodes. While dendritic cells (DC) pass MuHV-4 to lymph node B cells, subcapsular sinus macrophages (SSM), whic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01108-16
更新日期:2016-09-29 00:00:00
abstract::We previously demonstrated that chimeric porcine parvovirus-like particles (PPV:VLP) carrying heterologous epitopes, when injected intraperitoneally into mice without adjuvant, activate strong CD4(+) and CD8(+) T-cell responses specific for the foreign epitopes. In the present study, we investigated the immunogenicity...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2739-2744.1999
更新日期:1999-04-01 00:00:00
abstract::We have examined the pattern of RNA transcription on the L (late) DNA strand late in the simian virus 40 virus infectious cycle by separating pulse-labeled RNA chains according to size and hybridizing to an ordered series of DNA fragments obtained by restriction enzyme digestion. From this analysis, the 5' end of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.795-801.1978
更新日期:1978-12-01 00:00:00
abstract::Immediate-early viral gene products of human cytomegalovirus (HCMV) are derived from several genomic loci and largely serve to establish a cellular environment conducive to viral replication. We have further examined an unusual immediate-early transcript known as the 5-kb RNA, concluding that it is a stable intron enc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.13182-13189.2004
更新日期:2004-12-01 00:00:00
abstract:UNLABELLED:A balance between the functions of the influenza virus surface proteins hemagglutinin (HA) and neuraminidase (NA) is thought to be important for the transmission of viruses between humans. Here we describe two pandemic H1N1 viruses, A/swine/Virginia/1814-1/2012 and A/swine/Virginia/1814-2/2012 (pH1N1low-1 an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02537-14
更新日期:2015-06-01 00:00:00
abstract::Adenoviral RNA sequences synthesized in nuclei isolated during the late phase of productive infection comprise, besides virus-associated, VA, RNA, five major species, ranging in size from approximately 13S to 55S. The latter RNA species is of the length predicted if the major transcriptional unit expressed during the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.2.394-403.1979
更新日期:1979-11-01 00:00:00
abstract::Comparative surface feature analyses of the VP1 sequences of hepatitis A virus (HAV) and poliovirus type 1 allowed an alignment of the two sequences and an identification of probable HAV neutralization antigenic sites. A synthetic peptide containing the HAV-specific amino acid sequence of one of these sites induced an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.836-839.1985
更新日期:1985-09-01 00:00:00
abstract::To examine the mode of transmission of BK polyomavirus (BKV), urine samples were collected from Japanese-Americans in Los Angeles and from other southern Californians. Subtype I was the main subtype found in samples from both groups. The subtype I subgroup Ib-2, which is predominant in Europe, was the primary subgroup...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01018-07
更新日期:2007-12-01 00:00:00
abstract::Rabies virus (RV) vaccine strain-based vectors show significant promise as potential live-attenuated vaccines against human immunodeficiency virus type 1 (HIV-1). Here we describe a new RV construct that will also likely have applications as a live-attenuated or killed-particle immunogen. We have created a RV containi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12782-12794.2003
更新日期:2003-12-01 00:00:00
abstract::Some of the properties of a new human papovavirus, BK, have been examined. Host range studies of BK virus (BKV) showed human cells to be more sensitive to infection than monkey cells; human fetal brain cells appear to be highly sensitive to BKV, with the production of extensive cytopathology characterized by cytoplasm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.625-631.1973
更新日期:1973-09-01 00:00:00
abstract::Human metapneumovirus (hMPV) is a respiratory paramyxovirus of global clinical relevance. Despite the substantial knowledge generated during the last 10 years about hMPV infection, information regarding the activation of the immune response against this virus remains largely unknown. In this study, we demonstrated tha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01213-12
更新日期:2013-01-01 00:00:00
abstract::Influenza A H9N2 viruses are common poultry pathogens that occasionally infect swine and humans. It has been shown previously with H9N2 viruses that reassortment can generate novel viruses with increased transmissibility. Here, we demonstrate the modeling power of a novel transfection-based inoculation system to selec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02677-13
更新日期:2014-01-01 00:00:00
abstract::Complement, part of the innate immune system, acts to remove pathogens and unwanted host material. Complement is known to function in all tissues, including the central nervous system (CNS). In this study, we demonstrated the importance of the complement system within the CNS in the development of behavioral seizures ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00422-10
更新日期:2010-07-01 00:00:00
abstract::Dengue virus (DENV) is a major global pathogen that causes significant morbidity and mortality in tropical and subtropical areas worldwide. An improved understanding of the regions within the DENV genome and its encoded proteins that are required for the virus replication cycle will expedite the development of urgentl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01455-17
更新日期:2017-11-14 00:00:00
abstract::The vaccinia virus H2R gene (VACWR 100) is conserved in all sequenced members of the poxvirus family and encodes a protein with a predicted transmembrane domain and four invariant cysteines. A recombinant vaccinia virus, in which expression of the H2 protein is stringently regulated, was unable to replicate without in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.4744-4754.2005
更新日期:2005-04-01 00:00:00
abstract::Murine cytomegalovirus propagated in mouse embryo fibroblasts was purified by the following procedures. (i) Extracellular virus was concentrated by centrifugation at 100,000 x g for 90 min. (ii) The concentrated virus was passed through a Bio-Rad Bio-Gel A-15m column to eliminate contaminating materials smaller than 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.906-915.1976
更新日期:1976-03-01 00:00:00
abstract::Systemic application of first-generation adenovirus induces pathogenic effects in the liver. To begin unraveling the mechanisms underlying early liver toxicity after adenovirus infusion, particularly the role of macrophage activation and expression of viral genes in transduced target cells, first-generation adenovirus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8798-8807.1997
更新日期:1997-11-01 00:00:00
abstract::Purified nuclei from polyoma-infected mouse (3T3) cells were found to be greatly reduced in their ability to synthesize viral DNA in vitro when compared with a crude system consisting of an unfractionated hypotonic lysate of the infected cells. The synthetic capacity of the nuclei could be fully reconstituted when a h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.97-107.1975
更新日期:1975-01-01 00:00:00