Abstract:
:We have examined the pattern of RNA transcription on the L (late) DNA strand late in the simian virus 40 virus infectious cycle by separating pulse-labeled RNA chains according to size and hybridizing to an ordered series of DNA fragments obtained by restriction enzyme digestion. From this analysis, the 5' end of the nascent transcript occurs a short distance counterclockwise from 0.735. Transcription proceeds in a clockwise direction. There is equimolar transcription for at least 1,000 nucleotides beyond the 3' end of the mRNA (0.175). The 3' terminus for the largest molecules is in 0.43--0.655. Molecules which represent more than one circuit of the genome are not more than 1% of the total population of nascent molecules. Implications of these findings for models of the biogenesis of mRNA are discussed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ford JP,Hsu MTdoi
10.1128/JVI.28.3.795-801.1978subject
Has Abstractpub_date
1978-12-01 00:00:00pages
795-801issue
3eissn
0022-538Xissn
1098-5514journal_volume
28pub_type
杂志文章abstract::Bacteriophage phi105 is a temperate bacteriophage for Bacillus subtilis 168. Temperature-sensitive and plaque mutants of phi105 were isolated. The results of two- and three-factor crosses with these mutants suggest the vegetative map of phi105 to be circular. The location of prophage phi105 between bacterial markers p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.1.38-44.1969
更新日期:1969-01-01 00:00:00
abstract::Kinetic analysis showed that the Ki values and the Ki/Km ratios for mutated, recombinant M184V human immunodeficiency virus type 1 reverse transcriptase (RT) for (-)2'-dideoxy-3'-thiacytidine triphosphate (3TCTP) were 35-fold higher than the equivalent values for wild-type RT but only about twice as high as the equiva...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5642-5645.1996
更新日期:1996-08-01 00:00:00
abstract::Human T-cell lymphotropic/leukemia virus type 1 (HTLV-1) transforms human T cells in vitro, and Tax, a potent transactivator of viral and cellular genes, plays a key role in cell immortalization. Tax activity is mediated by interaction with cellular transcription factors including members of the CREB/ATF family, the N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8852-8860.1998
更新日期:1998-11-01 00:00:00
abstract::This study examines the persistence and fitness of multidrug-resistant (MDR) viruses acquired during primary human immunodeficiency virus infection (PHI). In four individuals, MDR infections persisted over the entire study period, ranging from 36 weeks to 5 years, in the absence of antiretroviral therapy. In stark con...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.1753-1761.2002
更新日期:2002-02-01 00:00:00
abstract::Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent epsilon-adenosine (epsilonA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(epsilonA) RNA was maintained intac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1108-1114.1974
更新日期:1974-11-01 00:00:00
abstract::Genetic and biochemical studies have suggested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses capsids, but its arrangement remained unknown. Here, we report the first visualization of a unique vertex in the Kaposi's sarcoma-associated herpesvirus (KSHV) capsid by cryoelec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02254-06
更新日期:2007-04-01 00:00:00
abstract::A mutation that disrupts the interaction between the NS2 protein of minute virus of mice and the nuclear export factor Crm1 results in a block to egress of mutant-generated full virions from the nucleus of infected murine cells. These mutants produce wild-type levels of monomer and dimer replicative DNA forms but are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3257-3266.2002
更新日期:2002-04-01 00:00:00
abstract::Antisera produced in mice recognize primarily type-specific antigenic determinants on both the major core protein, p30, and the major envelope proteins, gp70 and p15(E), of the endogenous leukemia viruses (MuLV) of BALB/c mice. Three different mouse sera were investigated in detail. (i) Antisera prepared in C57BL/6 mi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.345-349.1979
更新日期:1979-10-01 00:00:00
abstract::We report sequence hypervariability in the viral protein 1 (VP1) interaction domain of VP2 in the norovirus (NoV) genogroup II genotype 4 (GII.4) lineage on 3 levels: (i) the global evolution of pandemic/epidemic strains from the mid-1970s through post-2006, (ii) the local emergence of an epidemic strain, and (iii) an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00228-11
更新日期:2012-01-01 00:00:00
abstract::The ecotropic Cas-Br-E murine leukemia virus (MuLV) and its molecularly cloned derivative pBR-NE-8 MuLV are capable of inducing hind-limb paralysis and leukemia after inoculation into susceptible mice. T1 oligonucleotide fingerprinting, molecular hybridization, and restriction enzyme analysis previously showed that th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.910-919.1986
更新日期:1986-12-01 00:00:00
abstract::We have previously shown that two inhibitors specific for cellular cyclin-dependent kinases (cdks), Roscovitine (Rosco) and Olomoucine (Olo), block the replication of herpes simplex virus (HSV). Based on these results, we demonstrated that HSV replication requires cellular cdks that are sensitive to these drugs (L. M....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2107-2120.2000
更新日期:2000-03-01 00:00:00
abstract::Poorly cytopathic or noncytopathic viruses can escape immune surveillance and establish a chronic infection. Here we exploited the strategy of combining antiviral drug treatment with the induction of a neutralizing antibody response to avoid the appearance of neutralization-resistant virus variants. Despite the fact t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.5896-5901.2000
更新日期:2000-07-01 00:00:00
abstract::A viral mRNA of the late kinetic class expressed by murine cytomegalovirus (MCMV) contains an open reading frame (ORF) whose predicted protein, designated MCK-1, has homology to beta chemokines (M. R. MacDonald, X.-Y. Li, and H. W. Virgin IV, J. Virol. 71:1671-1678, 1997). The present study analyzed further the struct...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.3682-3691.1999
更新日期:1999-05-01 00:00:00
abstract::Murine leukemia viruses isolated from radiation-induced BALB/c leukemias were characterized with respect to viral proteins and RNA. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the viral structural proteins revealed that for p12, p15, p30, and gp70, three of four electrophoretic variants of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.661-670.1980
更新日期:1980-02-01 00:00:00
abstract::Most strains of human immunodeficiency virus type 1 (HIV-1) which have only been carried in vitro in peripheral blood mononuclear cells (primary isolates) can be neutralized by antibodies, but their sensitivity to neutralization varies considerably. To study the parameters that contribute to the differential neutraliz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.6988-6996.1998
更新日期:1998-09-01 00:00:00
abstract::Pretreatment with two biological response modifiers (BRM), OK-432 and PS-K, protected mice from lethal infection by murine cytomegalovirus (MCMV). This was evidenced by an increase in 50% lethal doses and a decrease in titers of infectious viruses replicated in the liver and spleen. Spleen cells from the BRM-treated m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.1.117-122.1984
更新日期:1984-07-01 00:00:00
abstract::The Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 57 (ORF57)-encoded protein (Mta) is a multifunctional regulator of viral gene expression. ORF57 is essential for viral replication, so elucidation of its molecular mechanisms is important for understanding KSHV infection. ORF57 has been implicated i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03459-12
更新日期:2013-04-01 00:00:00
abstract::Transcription of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) is activated through binding of the viral Tat protein to the trans-activating response (TAR) element at the 5' end of the nascent transcript. Whereas HIV type 1 (HIV-1) TAR folds a simple hairpin structure, the corresponding do...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00530-08
更新日期:2008-09-01 00:00:00
abstract::In resting CD4(+) T lymphocytes harboring human immunodeficiency virus type 1 (HIV-1), replication-competent virus persists in patients responding to highly active antiretroviral therapy (HAART). This small latent reservoir represents between 10(3) and 10(7) cells per patient. However, the efficiency of HIV-1 DNA-posi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10536-10542.2004
更新日期:2004-10-01 00:00:00
abstract::Cell fractionation and protein electrophoresis were used to study the intracellular sites of synthesis and intermediate structures in the assembly of the virion proteins of vesicular stomatitis virus. Each of the three major virion proteins assembled into virions through a separable pathway. The nucleocapsid (N) prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.1128-1139.1977
更新日期:1977-03-01 00:00:00
abstract::We report here that sex hormones modulate susceptibility to a sexually transmitted viral agent, herpes simplex virus type 2 (HSV-2), in a mouse model. Ovariectomized mice were administered either saline (control), estradiol (E(2)), progesterone (P(4)), or a combination of both estradiol and progesterone (E+P) and infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.3107-3116.2005
更新日期:2005-03-01 00:00:00
abstract::It was recently shown that the 7a protein of severe acute respiratory syndrome coronavirus induces biochemical changes associated with apoptosis. In this study, the mechanism by which the 7a protein induces apoptosis was examined. The 7a protein was tested for the ability to inhibit cellular gene expression because se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.2.785-793.2006
更新日期:2006-01-01 00:00:00
abstract::A limited period of chemotherapy during primary immunodeficiency virus infection might provide a long-term clinical benefit even if treatment is initiated at a time point when virus is already detectable in plasma. To evaluate this strategy, we infected rhesus macaques with the pathogenic simian/human immunodeficiency...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1704-1711.2000
更新日期:2000-02-01 00:00:00
abstract::A recombinant capripoxvirus vaccine containing a cDNA of the peste-des-petits-ruminants virus (PPRV) fusion protein gene was constructed. A quick and efficient method was used to select a highly purified recombinant virus clone. A trial showed that a dose of this recombinant as low as 0.1 PFU protected goats against c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1571-1577.2003
更新日期:2003-01-01 00:00:00
abstract::Carboxypeptidase D (gp180), one of many candidate receptors proposed for hepatitis B viruses (HBVs), was examined and found to be the actual cellular receptor for avian HBVs. This conclusion was based on the following observations: (i) gp180 was the only host protein that bound with high affinity to the pre-S ectodoma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8098-8104.1998
更新日期:1998-10-01 00:00:00
abstract::Ten bacteriophages that attack and lyse saprophytic strains of Bdellovibrio bacteriovorus were isolated. Morphological, serological, and host-range studies revealed that there were four different bdellovibrio phages present among the isolates. One of the phages lysed a strain of B. bacteriovorus that requires the pres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.3.516-523.1972
更新日期:1972-09-01 00:00:00
abstract::The Vpr protein, encoded by the human immunodeficiency virus type 1 (HIV-1) genome, is one of the nonstructural proteins packaged in large amounts into viral particles. We have previously reported that Vpr associates with the DNA repair enzyme uracil DNA glycosylase (UDG). In this study, we extended these observations...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1682-1688.1999
更新日期:1999-02-01 00:00:00
abstract::Influenza virus particles bind rapidly to vesicular stomatitis, Sindbis, or Rauscher murine leukemia virus particles, forming mixed aggregates demonstrable by electron microscopy. The normal hemagglutinating property of influenza virus is inhibited by these viruses, providing a rapid quantitative assay. Prior treatmen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1307-1309.1974
更新日期:1974-11-01 00:00:00
abstract::The blood group P antigen, known to be abundantly expressed on erythroid cells, has been reported to be the cellular receptor for parvovirus B19. We have described the development of recombinant parvovirus B19 vectors with which high-efficiency, erythroid lineage-restricted transduction can be achieved (S. Ponnazhagan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4110-4116.2001
更新日期:2001-05-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) infection of natural hosts is characterized by nonpathogenic chronic viremia, maintenance of gastrointestinal epithelial barrier integrity, and low numbers of target cells. Assessment of cell-associated virus load in T cell subsets in multiple anatomic compartments of chronically SI...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01556-12
更新日期:2012-10-01 00:00:00