Abstract:
:Human immunodeficiency virus clade C (HIV-C) accounts for >56% of all HIV infections worldwide. To investigate vaccine safety and efficacy in nonhuman primates, a pathogenic, R5-tropic, neutralization-sensitive simian-human immunodeficiency virus (SHIV) carrying HIV-C env would be desirable. We have constructed SHIV-2873Ni, an R5-tropic SHIV carrying a primary pediatric HIV-C env gene isolated from a 2-month-old Zambian infant, who died within 1 year of birth. SHIV-2873Ni was constructed using SHIV-1157ipd3N4 (R. J. Song, A. L. Chenine, R. A. Rasmussen, C. R. Ruprecht, S. Mirshahidi, R. D. Grisson, W. Xu, J. B. Whitney, L. M. Goins, H. Ong, P. L. Li, E. Shai-Kobiler, T. Wang, C. M. McCann, H. Zhang, C. Wood, C. Kankasa, W. E. Secor, H. M. McClure, E. Strobert, J. G. Else, and R. M. Ruprecht. J. Virol. 80:8729-8738, 2006) as the backbone, since the latter contains additional NF-kappaB sites in the long terminal repeats to enhance viral replicative capacity. The parental virus, SHIV-2873Ni, was serially passaged through five rhesus monkeys (RMs); SHIV-2873Nip, the resulting passaged virus, was reisolated from the fourth recipient about 1 year postinoculation. SHIV-2873Nip was replication competent in RM peripheral blood mononuclear cells of all random donors tested and was exclusively R5 tropic, and its env gene clustered with HIV-C by phylogenetic analysis; its moderate [corrected] sensitivity to neutralization led to classification as a tier 2 [corrected] virus. Indian-origin RMs were inoculated by different mucosal routes, resulting in high peak viral RNA loads. Signs of virus-induced disease include depletion of gut CD4(+) T lymphocytes, loss of memory T cells in blood, and thrombocytopenia that resulted in fatal cerebral hemorrhage. SHIV-2873Nip is a highly replication-competent, mucosally transmissible, pathogenic R5-tropic virus that will be useful to study viral pathogenesis and to assess the efficacy of immunogens targeting HIV-C Env.
journal_name
J Viroljournal_title
Journal of virologyauthors
Siddappa NB,Song R,Kramer VG,Chenine AL,Velu V,Ong H,Rasmussen RA,Grisson RD,Wood C,Zhang H,Kankasa C,Amara RR,Else JG,Novembre FJ,Montefiori DC,Ruprecht RMdoi
10.1128/JVI.02066-08subject
Has Abstractpub_date
2009-02-01 00:00:00pages
1422-32issue
3eissn
0022-538Xissn
1098-5514pii
JVI.02066-08journal_volume
83pub_type
杂志文章abstract::The immediate-early 1 and 2 (IE1 and IE2, respectively) proteins of human cytomegalovirus are known transcription factors, which regulate the expression of viral and cellular genes. Transcriptional activation by IE2 is dependent on the presence of a TATA motif in target promoters, and IE2 can interact directly with th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.4028-4037.1996
更新日期:1996-06-01 00:00:00
abstract::PM2 DNA was prepared with different superhelical densities (sigma) in order to examine the relationship betweenn supercoiling and the occurrence of a region(s) of unpaired bases in this DNA. A previous study showed that CH3HgOH reacts with native superhelical PM2 DNA more rapidly than the nicked form II. This evaluati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.1.195-204.1976
更新日期:1976-04-01 00:00:00
abstract::Fluorosulfonylbenzoyl 5'-adenosine (FSBA) bound to one site in simian virus 40 large T antigen (T) and covalently modified greater than 95% of the molecules in a complete reaction. This analog for ATP specifically cross-links to the Mg-phosphate pocket in ATP-binding sites. Cyanogen bromide cleavage and tryptic digest...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.4939-4947.1990
更新日期:1990-10-01 00:00:00
abstract::Strategies were developed to insert the neo resistance marker into the junction between L DNA and the right terminal repetitive H DNA of herpesvirus saimiri. Recombinant viruses were selectable in permissive epithelioid cultures. The human T-cell line Jurkat could be infected persistently with the Neor virus; the cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1818-1821.1989
更新日期:1989-04-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) R5 isolates that predominantly use CCR5 as a coreceptor are frequently described as macrophage tropic. Here, we compare macrophage tropism conferred by HIV-1 R5 envelopes that were derived directly by PCR from patient tissue. This approach avoids potentially selective cultur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02328-05
更新日期:2006-07-01 00:00:00
abstract::The gene encoding the glycoprotein Marek's disease herpesvirus A antigen (MDHV-A) precursor polypeptide pr47 was delineated by using Northern blot (RNA blot) analysis and hybrid selection of its mRNA with cloned MDHV DNA, cell-free translation of the mRNA, and immunoprecipitation of the polypeptide. The resulting piec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2614-2620.1987
更新日期:1987-08-01 00:00:00
abstract::Whole-body bioimaging was used to study dissemination of vaccinia virus (VACV) in normal and in immune deficient (nu(-)/nu(-)) mice protected from lethality by postchallenge administration of ST-246. Total fluxes were recorded in the liver, spleen, lungs, and nasal cavities of live mice after intranasal infection with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03426-12
更新日期:2013-05-01 00:00:00
abstract::Recombination is one of the driving forces of viral evolution. RNA recombination events among similar RNA viruses are frequent, although RNA recombination could also take place among unrelated viruses. In this paper, we have established efficient interviral recombination systems based on yeast and plants. We show that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01015-19
更新日期:2019-12-12 00:00:00
abstract::Valproic acid (VPA) is a short-chain fatty acid commonly used for treatment of neurological disorders. As VPA can interfere with cellular lipid metabolism, its effect on the infection of cultured cells by viruses of seven viral families relevant to human and animal health, including eight enveloped and four nonenvelop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01717-10
更新日期:2011-02-01 00:00:00
abstract::We have previously reported that infection of 9- to 13-day-old chicken embryos with RAV-1 results in rapid development of a novel B-cell lymphoma in which proviral insertion has activated expression of the c-myb gene (E. Pizer and E. H. Humphries, J. Virol. 63:1630-1640, 1989). The biological properties of these B-cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.1.512-523.1992
更新日期:1992-01-01 00:00:00
abstract::Viral hemorrhagic septicemia virus (VHSV) infections cause high losses in cultured rainbow trout in Europe. Attempts to produce a recombinant vaccine based on the transmembrane glycoprotein (G protein) have indicated that proper folding is important for the antigenicity and immunogenicity of the protein. The present s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10189-10196.1998
更新日期:1998-12-01 00:00:00
abstract::In immunocompromised patients, infection with Kaposi's sarcoma-associated herpesvirus (KSHV) can give rise to Kaposi's sarcoma and several lymphoproliferative disorders. In these tumors, KSHV establishes a latent infection in many of the rapidly proliferating and morphologically abnormal cells. Only a few viral gene p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.10074-10085.2004
更新日期:2004-09-01 00:00:00
abstract:UNLABELLED:The HIV-1 glycoprotein 41 promotes fusion of the viral membrane with that of the target cell. Structural, biochemical, and biophysical studies suggest that its membrane-proximal external region (MPER) may interact with the HIV-1 membrane and induce its disruption and/or deformation during the process. Howeve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02151-14
更新日期:2014-11-01 00:00:00
abstract::Modification of proteins by ubiquitin is essential for numerous cellular processes. The RING-H2 finger motif has been implicated in ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Four proteins, WSSV199, WSSV222, WSSV249, and WSSV403, from white spot syndrome virus (WSSV) contain the RING-H2 motif. Here we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.8764-8772.2005
更新日期:2005-07-01 00:00:00
abstract::Using quantitative RNase protection assays, we have monitored the appearance of mRNAs generated during lytic infection of tightly synchronized murine cells by the autonomous parvovirus minute virus of mice. Our results demonstrate that transcripts from the P4 promoter can be detected prior to those from the P39 promot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.4.1448-1451.1988
更新日期:1988-04-01 00:00:00
abstract::Spontaneous deletion mutants of satellite bacteriophage P4 have been isolated and characterized. All of the deletions analyzed that were between 850 and 1,700 base pairs long are within the region nonessential for P4 lytic development; some of them cover the cII or the gop locus. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.233-235.1985
更新日期:1985-04-01 00:00:00
abstract::A functional preinitiation transcription complex was formed by incubating vaccinia virus early transcription factor VETF and RAP94+ RNA polymerase with an early promoter template immobilized on paramagnetic particles. A preferred order of assembly, VETF followed by RNA polymerase, was demonstrated by stepwise addition...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.6052-6056.1994
更新日期:1994-09-01 00:00:00
abstract::We report the complete genome sequence of Pseudomonas aeruginosa siphophage MP1412, which displays synteny to those of P. aeruginosa phages M6 and YuA. However, the presence of two homing endonucleases of the GIY-YIG family is unique to MP1412, suggesting their unique role in the phage life cycle of the bacterial host...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01403-12
更新日期:2012-09-01 00:00:00
abstract::Bovine herpesvirus 1 (BHV-1) infected cell protein 0 (bICP0) stimulates productive infection, in part by activating viral gene expression. The C(3)HC(4) zinc RING finger of bICP0 is crucial for activating viral transcription and productive infection. In this study, we used a bacterial artificial chromosome containing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01348-08
更新日期:2008-12-01 00:00:00
abstract:UNLABELLED:Human T-cell lymphotropic virus type 1 (HTLV-1) and HTLV-2 encode auxiliary proteins that play important roles in viral replication, viral latency, and immune escape. The presence of auxiliary protein-encoding open reading frames (ORFs) in HTLV-3, the latest HTLV to be discovered, is unknown. Simian T-cell l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02150-14
更新日期:2015-01-15 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.2806-2814.1998
更新日期:1998-04-01 00:00:00
abstract::Efforts to induce broadly reactive immunity against human immunodeficiency virus type 1 (HIV-1) have been impaired by the extent of sequence variation exhibited by this lentivirus. Cytotoxic T lymphocytes (CTL) specific for other viruses such as influenza virus have been shown to mediate immunity against divergent vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3145-3153.1994
更新日期:1994-05-01 00:00:00
abstract::A hydrophilic region consisting of strikingly clustered charged amino acids is present at the center of human immunodeficiency virus type 1 (HIV-1) Vif. In this study, the role for this central hydrophilic region (E(88)WRKKR(93)) in the virus replication in nonpermissive H9 cells was investigated by extensive deletion...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1626-1632.2003
更新日期:2003-01-01 00:00:00
abstract::Normal replication of Moloney murine leukemia virus (MoMLV) requires the integration of a DNA copy of the viral RNA genome into a chromosome of the host. In this work, we characterize the DNA sequences at the ends of the linear proviral precursor that are required for integration in the presence of MoMLV integration p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5645-5648.1990
更新日期:1990-11-01 00:00:00
abstract::We have purified the seven virus-specific RNAs which were previously shown to be induced in Sac(-) cells upon infection with mouse hepatitis virus strain A59 (W. J. M. Spaan, P. J. M. Rottier, M. C. Horzinek, and B. A. M. van der Zeijst, Virology 108:424-434, 1981). The individual RNAs, prepared by agarose gel electro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.20-26.1981
更新日期:1981-04-01 00:00:00
abstract::Reverse transcription in avian sarcoma virus (ASV) initiates from the 3' end of a tRNA(Trp) primer, which anneals near the 5' end of the RNA genome. The region around the primer-binding site (PBS) forms an elaborate stem structure composed of the U5-inverted repeat (U5-IR) stem, the U5-leader stem, and the association...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7571-7577.2002
更新日期:2002-08-01 00:00:00
abstract:UNLABELLED:The cytomegaloviruses (CMVs) are among the most genetically complex mammalian viruses, with viral genomes that often exceed 230 kbp. Manipulation of cytomegalovirus genomes is largely performed using infectious bacterial artificial chromosomes (BACs), which necessitates the maintenance of the viral genome in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00139-16
更新日期:2016-07-11 00:00:00
abstract::Interferon-induced proteins (IFPs) exert multiple functions corresponding to diverse interferon signals. However, the intracellular functions of many IFPs are not fully characterized. Here, we report that IFP35, a member of the IFP family with a molecular mass of 35 kDa, can interact with the bovine Tas (BTas) regulat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02249-07
更新日期:2008-05-01 00:00:00
abstract::The temporal regulation of DNA replication is thought to be important for chromosome organization and genome stability. We show here that Epstein-Barr virus (EBV) genomes replicate in mid- to late S phase and that agents that accelerate replication timing of EBV reduce viral genome stability. Hydroxyurea (HU) treatmen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02115-08
更新日期:2009-03-01 00:00:00
abstract::Endocytosis and endosomal trafficking regulate the proteins targeted to the plasma membrane and play essential roles in diverse cellular processes, including responses to pathogen attack. Here, we report the identification of Glycine max (soybean) endocytosis dynamin-like protein 5A (GmSDL5A) associated with purified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01320-18
更新日期:2018-11-12 00:00:00