Abstract:
:One-step growth determinations were performed with five strains of rotavirus in HepG2, a cell line derived from human liver. Three virus strains (SA11-C13, SA11-C14, and RRV) replicated in HepG2 cells and attained yields 10- to 100-fold above input titers. Two virus strains (B223 and SA11-4F) failed to replicate above input titer. Analysis of reassortants that segregated the genes of parental virus pairs able and unable to replicate revealed that the HepG2 cell growth phenotype segregated with genome segment 4. Immunofluorescence analysis of infected HepG2 cells showed that the production of detectable antigen correlated with the growth phenotype and also segregated with genome segment 4. Thus, we conclude that (i) some virus strains were capable of replication in cultured liver cells while other strains could not replicate under identical conditions and that (ii) the inability of some virus strains to replicate resulted from a segment 4-associated block in replication before protein synthesis. These results are discussed in terms of what is known of the functions of VP4.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ramig RF,Galle KLdoi
10.1128/JVI.64.3.1044-1049.1990subject
Has Abstractpub_date
1990-03-01 00:00:00pages
1044-9issue
3eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::The DNA of normal chicken embryos contains sequences related to the avian leukosis-sarcoma viruses. RNA-dependent DNA polymerase of these viruses is encoded by a genetic element known as the pol gene. The nature of the endogenous virus pol gene in chicken cells was investigated by testing its ability to participate in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.856-862.1979
更新日期:1979-03-01 00:00:00
abstract::Structural proteins of temperature-sensitive (ts) mutants of vesicular stomatitis virus, Indiana serotype, were compared with those of wild-type and revertant virions by electrophoresis on polyacrylamide gels of partial digests with Staphylococcus aureus V8 protease. Mutants of complementation groups III (tsG31 and ts...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.248-255.1981
更新日期:1981-01-01 00:00:00
abstract::Infections of fungi by mycoviruses are often symptomless but sometimes also fatal, as they perturb sporulation, growth, and, if applicable, virulence of the fungal host. Hypovirulence-inducing mycoviruses, therefore, represent a powerful means to defeat fungal epidemics on crop plants. Infection with Fusarium graminea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00326-18
更新日期:2018-08-16 00:00:00
abstract::Although recombinant human adenovirus (HAV)-based vectors offer several advantages for somatic gene therapy and vaccination over other viral vectors, it would be desirable to develop alternative vectors with prolonged expression and decreased toxicity. Toward this objective, a replication-defective bovine adenovirus t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9137-9144.1999
更新日期:1999-11-01 00:00:00
abstract::Herpes simplex virus type 1 packages its DNA genome into a precursor capsid, referred to as the procapsid. Of the three capsid-associated DNA-packaging proteins, UL17, UL25, and UL6, only UL17 and UL6 appear to be components of the procapsid, with UL25 being added subsequently. To determine whether the association of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2118-2126.2006
更新日期:2006-03-01 00:00:00
abstract::The structures of DNAs present in various intracellular forms of simian virus 40 (SV40) nucleoprotein complexes were analyzed by micrococcal nuclease digestion. The results showed that the 70S SV40 chromatin was completely sensitive to nuclease digestion, whereas CsCl gradient-purified mature virion was completely res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.603-609.1982
更新日期:1982-11-01 00:00:00
abstract::The Arabidopsis flowering locus T (FT) gene encodes the mobile florigen essential for floral induction. While movement of the FT protein has been shown to occur within plants, systemic spread of FT mRNA remains to be unequivocally demonstrated. Utilizing novel RNA mobility assay vectors based on two distinct movement-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02346-08
更新日期:2009-04-01 00:00:00
abstract:UNLABELLED:Hepatitis C virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma in humans. We showed previously that HCV induces autophagy for viral persistence by preventing the innate immune response. Knockdown of autophagy reduces extracellular HCV release, although the precis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02383-15
更新日期:2015-11-18 00:00:00
abstract::Venezuelan equine encephalitis (VEE) virus antigenic subtypes and varieties are considered either epidemic/epizootic or enzootic. In addition to epidemiological differences between the epidemic and enzootic viruses, several in vitro and in vivo laboratory markers distinguishing the viruses have been identified, includ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4258-4263.2000
更新日期:2000-05-01 00:00:00
abstract:UNLABELLED:Hepatitis B, which caused by hepatitis B virus (HBV) infection, remains a major health threat worldwide. Hepatic injury and regeneration from chronic inflammation are the main driving factors of liver fibrosis and cirrhosis in chronic hepatitis B. Proinflammatory tumor necrosis factor alpha (TNF-α) has been ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03106-14
更新日期:2015-02-01 00:00:00
abstract::Adenovirus has a linear, double-stranded DNA genome that is perceived by the cellular Mre11-Rad50-Nbs1 (MRN) DNA repair complex as a double-strand break. If unabated, MRN elicits a double-strand break repair response that blocks viral DNA replication and ligates the viral genomes into concatemers. There are two sets o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02540-10
更新日期:2011-05-01 00:00:00
abstract::We studied the temporal relationship between human immunodeficiency type 1 (HIV-1) quasispecies in tissues and in peripheral blood mononuclear cells (PBMC) of infected individuals. Sequential PBMC and tissue samples from various organs obtained at autopsy from three patients who died of AIDS-related complications were...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.488-496.1998
更新日期:1998-01-01 00:00:00
abstract::Each influenza season, a set of wild-type viruses, representing one H1N1, one H3N2, and one to two influenza B isolates, are selected for inclusion in the annual seasonal influenza vaccine. In order to develop broadly reactive subtype-specific influenza vaccines, a methodology called computationally optimized broadly ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01581-17
更新日期:2017-11-30 00:00:00
abstract::The E7 oncoprotein of human papillomavirus 16 (HPV16) transforms basal and suprabasal cervical epithelial cells and is a tumor-specific antigen in cervical carcinoma, to which immunotherapeutic strategies aimed at cytotoxic T-lymphocyte (CTL) induction are currently directed. By quantifying major histocompatibility co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5985-5997.2001
更新日期:2001-07-01 00:00:00
abstract::Rfv3 is an autosomal dominant gene that influences the recovery of resistant mice from Friend retrovirus (FV) infection by limiting viremia and promoting a more potent neutralizing antibody response. We previously reported that Rfv3 is encoded by Apobec3, an innate retrovirus restriction factor. However, it was recent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01838-10
更新日期:2011-01-01 00:00:00
abstract::Productive replication of human immunodeficiency virus type 1 (HIV-1) occurs efficiently only in humans. The posttranscriptional stages of the HIV-1 life cycle proceed poorly in mouse cells, with a resulting defect in viral assembly and release. Previous work has shown that the presence of human chromosome 2 increases...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01970-12
更新日期:2012-11-01 00:00:00
abstract::A novel phage lambda mutation, called dc10, which interferes with proper lambda head assembly has been isolated and characterized. Phage lambda carrying this mutation is (i) unable to form plaques at 30 or 37 degrees C but does so at 42 degrees C and (ii) unable to form plaques at 42 degrees C on pN-constitutive hosts...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.2.782-788.1979
更新日期:1979-02-01 00:00:00
abstract::A strategy involving polymerase chain reaction amplification of cDNAs was designed to study the expression of the hamster polyomavirus (HaPV) early region in HaPV-transformed rat fibroblasts, productively HaPV-infected cells, and HaPV-induced lymphoma. We identified three mRNAs resulting from alternative splicing of o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2495-2504.1992
更新日期:1992-04-01 00:00:00
abstract::Retrovirus replication requires the production of both spliced and unspliced viral RNA from a single RNA transcript. In contrast, cellular RNA precursors with introns almost completely spliced. The cis elements and virus-coded trans factors which determine the extent to which Rous sarcoma virus RNA is spliced to src m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1669-1676.1989
更新日期:1989-04-01 00:00:00
abstract::Recombination is one of the driving forces of viral evolution. RNA recombination events among similar RNA viruses are frequent, although RNA recombination could also take place among unrelated viruses. In this paper, we have established efficient interviral recombination systems based on yeast and plants. We show that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01015-19
更新日期:2019-12-12 00:00:00
abstract::A adenovirus type 5 host range mutant (hr440) has been isolated which is defective in a splicing event required to generate the middle-sized mRNA from early region 1A. This defect has been ascribed to two adjacent nucleotide changes which lie five and six nucleotides from the 5' splice site for this mRNA (Solnick, Nat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.106-113.1982
更新日期:1982-04-01 00:00:00
abstract::Signal peptidase cleavage at the C-prM junction in the flavivirus structural polyprotein is inefficient in the absence of the cytoplasmic viral protease, which catalyzes cleavage at the COOH terminus of the C protein. The signal peptidase cleavage occurs efficiently in circumstances where the C protein is deleted or i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2141-2149.1998
更新日期:1998-03-01 00:00:00
abstract::Adenovirus deletion mutants were studied to examine the influence of the 5' untranslated sequence on the translation of early region 1A mRNAs. Alterations of the 5' untranslated sequence, including complete deletion of the wild-type 5' untranslated sequence, did not significantly affect the rate of translation. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.884-888.1984
更新日期:1984-09-01 00:00:00
abstract::The genome of influenza A virus consists of eight single-strand negative-sense RNA segments, each comprised of a coding region and a noncoding region. The noncoding region of the NS segment is thought to provide the signal for packaging; however, we recently showed that the coding regions located at both ends of the h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3766-3774.2005
更新日期:2005-03-01 00:00:00
abstract::In various positive-sense single-stranded RNA viruses, a low-fidelity viral RNA-dependent RNA polymerase (RdRp) confers attenuated phenotypes by increasing the mutation frequency. We report a negative-sense single-stranded RNA virus RdRp mutant strain with a mutator phenotype. Based on structural data of RdRp, rationa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00834-19
更新日期:2019-10-29 00:00:00
abstract::We have developed a system in which vesicular stomatitis virus (VSV) minigenomes encoding viral structural proteins can be expressed from plasmids. These RNAs can be replicated, transcribed, and packaged into infectious particles when coexpressed with the other VSV proteins. The minigenomes contain either the glycopro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.2946-2953.1995
更新日期:1995-05-01 00:00:00
abstract::It was recently shown that the 7a protein of severe acute respiratory syndrome coronavirus induces biochemical changes associated with apoptosis. In this study, the mechanism by which the 7a protein induces apoptosis was examined. The 7a protein was tested for the ability to inhibit cellular gene expression because se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.2.785-793.2006
更新日期:2006-01-01 00:00:00
abstract::Hepatitis C virus (HCV) is an important human pathogen associated with chronic liver disease. Recently, based on a genotype 2a isolate, tissue culture systems supporting complete replication and infectious virus production have been developed. In this study, we used cell culture-produced infectious HCV to analyze the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1734-1741.2006
更新日期:2006-02-01 00:00:00
abstract::Recombination studies have established that retroviral long terminal repeats (LTRs) are important genetic determinants of the viral capacity to induce hematopoietic tumors and to specify the type of cell making up the tumor. Plasmids containing LTRs of several murine leukemia viruses linked to the chloramphenicol acet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.1067-1072.1987
更新日期:1987-04-01 00:00:00
abstract::The alphavirus Semliki Forest virus (SFV) infects cells through low-pH-induced membrane fusion mediated by the E1 protein, a class II virus membrane fusion protein. During fusion, E1 inserts into target membranes via its hydrophobic fusion loop and refolds to form a stable E1 homotrimer. Mutation of a highly conserved...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00167-06
更新日期:2006-06-01 00:00:00