Generation of multiple replication-competent retroviruses through recombination between PreXMRV-1 and PreXMRV-2.


:We previously identified two novel endogenous murine leukemia virus proviruses, PreXMRV-1 and PreXMRV-2, and showed that they most likely recombined during xenograft passaging of a human prostate tumor in mice to generate xenotropic murine leukemia virus-related virus (XMRV). To determine the recombination potential of PreXMRV-1 and PreXMRV-2, we examined the generation of replication-competent retroviruses (RCRs) over time in a cell culture system. We observed that either virus alone was noninfectious and the RNA transcripts of the viruses were undetectable in the blood and spleen of nude mice that carry them. To determine their potential to generate RCRs through recombination, we transfected PreXMRV-1 and PreXMRV-2 into 293T cells and used the virus produced to infect fresh cells; the presence of reverse transcriptase activity at 10 days postinfection indicated the presence of RCRs. Population sequencing of proviral DNA indicated that all RCRs contained the gag and 5' half of pol from PreXMRV-2 and the long terminal repeat, 3' half of pol (including integrase), and env from PreXMRV-1. All crossovers were within sequences of at least 9 identical nucleotides, and crossovers within each of two selected recombination zones of 415 nucleotides (nt) in the 5' untranslated region and 982 nt in pol were required to generate RCRs. A recombinant with the same genotype as XMRV was not detected, and our analysis indicates that the probability of generating an identical RCR is vanishingly small. In addition, the studies indicate that the process of RCR formation is primarily driven by selection for viable cis and trans elements from the parental proviruses.


J Virol


Journal of virology


Delviks-Frankenberry K,Paprotka T,Cingöz O,Wildt S,Hu WS,Coffin JM,Pathak VK




Has Abstract


2013-11-01 00:00:00














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    abstract::Particular interest in human T lymphocyte lymphoma/leukemia virus (HTLV) derives from the close association of HTLV with several types of human mature T lymphocyte malignancies and the strong possibility that HTLV is the causative agent of this group of leukemias and lymphomas. This is the first report to show that HT...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Moore JL,Poiesz BJ,Zamkoff KW,Merl SA,Hanna S,Gazdar AF,Comis RL

    更新日期:1985-02-01 00:00:00

  • Identification of a New Benzimidazole Derivative as an Antiviral against Hepatitis C Virus.

    abstract:UNLABELLED:Aminoquinolines and piperazines, linked or not, have been used successfully to treat malaria, and some molecules of this family also exhibit antiviral properties. Here we tested several derivatives of 4-aminoquinolines and piperazines for their activity against hepatitis C virus (HCV). We screened 11 molecul...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Vausselin T,Séron K,Lavie M,Mesalam AA,Lemasson M,Belouzard S,Fénéant L,Danneels A,Rouillé Y,Cocquerel L,Foquet L,Rosenberg AR,Wychowski C,Meuleman P,Melnyk P,Dubuisson J

    更新日期:2016-09-12 00:00:00

  • De novo synthesis of N and P proteins as a key step in Sendai virus gene expression.

    abstract::Among the members of the paramyxovirus family, the transcription process and the components involved have been studied under in vitro conditions thus far. Here, we reexamined the function of the viral RNA-dependent RNA polymerase through infection studies with Sendai virus (SeV) N and P deletion (Delta) mutants. To el...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wiegand MA,Bossow S,Schlecht S,Neubert WJ

    更新日期:2007-12-01 00:00:00

  • Adenovirus-mediated p21((WAF1/SDII/CIP1)) gene transfer induces apoptosis of human cervical cancer cell lines.

    abstract::p21((WAF1/SDII/CIP1)) (p21) arrests cell growth by inhibiting cyclin-depend kinases. To explore the potential of using p21 for the gene therapy of cervical cancer, we infected human papillomavirus (HPV)-positive cervical cancer cells (HeLa, SiHa, and Z172) and HPV-negative cervical cancer cells (C33A) with recombinant...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Tsao YP,Huang SJ,Chang JL,Hsieh JT,Pong RC,Chen SL

    更新日期:1999-06-01 00:00:00

  • Replication of JC Virus DNA in the G144 Oligodendrocyte Cell Line Is Dependent Upon Akt.

    abstract::Progressive multifocal leukoencephalopathy (PML) is an often-fatal demyelinating disease of the central nervous system. PML results when oligodendrocytes within immunocompromised individuals are infected with the human JC virus (JCV). We have identified an oligodendrocyte precursor cell line, termed G144, that support...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Peterson JN,Lin B,Shin J,Phelan PJ,Tsichlis P,Schwob JE,Bullock PA

    更新日期:2017-09-27 00:00:00

  • Affinity Purification of the Hepatitis C Virus Replicase Identifies Valosin-Containing Protein, a Member of the ATPases Associated with Diverse Cellular Activities Family, as an Active Virus Replication Modulator.

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    pub_type: 杂志文章


    authors: Yi Z,Fang C,Zou J,Xu J,Song W,Du X,Pan T,Lu H,Yuan Z

    更新日期:2016-10-14 00:00:00

  • Comparative ultrastructural study of four reticuloendothelias viruses.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kang CY,Wong TC,Holmes KV

    更新日期:1975-10-01 00:00:00

  • Weak cis and trans Interactions of the Hemagglutinin with Receptors Trigger Fusion Proteins of Neuropathogenic Measles Virus Isolates.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Shirogane Y,Hashiguchi T,Yanagi Y

    更新日期:2020-01-06 00:00:00

  • Initial B-cell responses to transmitted human immunodeficiency virus type 1: virion-binding immunoglobulin M (IgM) and IgG antibodies followed by plasma anti-gp41 antibodies with ineffective control of initial viremia.

    abstract::A window of opportunity for immune responses to extinguish human immunodeficiency virus type 1 (HIV-1) exists from the moment of transmission through establishment of the latent pool of HIV-1-infected cells. A critical time to study the initial immune responses to the transmitted/founder virus is the eclipse phase of ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Tomaras GD,Yates NL,Liu P,Qin L,Fouda GG,Chavez LL,Decamp AC,Parks RJ,Ashley VC,Lucas JT,Cohen M,Eron J,Hicks CB,Liao HX,Self SG,Landucci G,Forthal DN,Weinhold KJ,Keele BF,Hahn BH,Greenberg ML,Morris L,Karim S

    更新日期:2008-12-01 00:00:00

  • N-terminal mutants of herpes simplex virus type 2 gH are transported without gL but require gL for function.

    abstract::Glycoprotein H (gH) is conserved among all herpesviruses and is essential for virus entry and cell fusion along with gL, gB, and, in most alphaherpesviruses, gD. Within the gH/gL heterodimer, it is thought that gH accounts for the fusion function and gL acts as a chaperone for the folding and transport of gH. Here, we...

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    pub_type: 杂志文章


    authors: Cairns TM,Friedman LS,Lou H,Whitbeck JC,Shaner MS,Cohen GH,Eisenberg RJ

    更新日期:2007-05-01 00:00:00

  • Virion RNA polymerases of two salmonid rhabdoviruses.

    abstract::RNA-dependent RNA polymerases were found to be associated with two salmonid rhabdoviruses: infectious hematopoietic necrosis (IHN) virus and the virus of hemorrhagic septicemia (VHS). The protein composition of these rhabdoviruses closely resembles that of rabies virus rather than that of vesicular stomatitis virus (M...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: McAllister PE,Wagner RR

    更新日期:1977-06-01 00:00:00

  • Pentosan Polysulfate: a Novel Glycosaminoglycan-Like Molecule for Effective Treatment of Alphavirus-Induced Cartilage Destruction and Inflammatory Disease.

    abstract:UNLABELLED:Arthritogenic alphaviruses such as Ross River virus (RRV) and chikungunya virus (CHIKV) cause large-scale epidemics of severe musculoskeletal disease and have been progressively expanding their global distribution. Since its introduction in July 2014, CHIKV now circulates in the United States. The hallmark o...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Herrero LJ,Foo SS,Sheng KC,Chen W,Forwood MR,Bucala R,Mahalingam S

    更新日期:2015-08-01 00:00:00

  • Genetic subpopulations of Rift Valley fever virus strains ZH548 and MP-12 and recombinant MP-12 strains.

    abstract::Rift Valley fever virus strain MP-12 was generated by serial plaque passages of parental strain ZH548 12 times in MRC-5 cells in the presence of a chemical mutagen, 5-fluorouracil. As a result, MP-12 encoded 4, 9, and 10 mutations in the S, M, and L segments, respectively. Among them, mutations in the M and L segments...

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    pub_type: 杂志文章


    authors: Lokugamage N,Freiberg AN,Morrill JC,Ikegami T

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  • Inhibition of human immunodeficiency virus type 1 and type 2 Tat function by transdominant Tat protein localized to both the nucleus and cytoplasm.

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    pub_type: 杂志文章


    authors: Orsini MJ,Debouck CM

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  • Herpes simplex virus 1 recombinants with noninverting genomes frozen in different isomeric arrangements are capable of independent replication.

    abstract::Herpes simplex virus 1 genome consists of two covalently linked components, L and S, that invert relative to each other to yield four equimolar isomeric populations designated P (prototype), Is (inversion of S component), Il (inversion of L component), and Ils (inversion of L and S components) differing in the orienta...

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    pub_type: 杂志文章


    authors: Jenkins FJ,Roizman B

    更新日期:1986-08-01 00:00:00

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    abstract::Proviral clones of human immunodeficiency virus type 1 which contained single amino acid changes in the envelope V3 region were constructed. PCR amplification of Sup-T1 T cells transfected with one such mutant, G312T, revealed low levels of virus that resulted in the generation of a revertant virus, in which an alanin...

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    pub_type: 杂志文章


    authors: Morris JF,Sternberg EJ,Gutshall L,Petteway SR Jr,Ivanoff LA

    更新日期:1994-12-01 00:00:00

  • Comparative seroprevalence and immunogenicity of six rare serotype recombinant adenovirus vaccine vectors from subgroups B and D.

    abstract::Recombinant adenovirus serotype 5 (rAd5) vector-based vaccines are currently being developed for both human immunodeficiency virus type 1 and other pathogens. The potential limitations associated with rAd5 vectors, however, have led to the construction of novel rAd vectors derived from rare Ad serotypes. Several rare ...

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    pub_type: 杂志文章


    authors: Abbink P,Lemckert AA,Ewald BA,Lynch DM,Denholtz M,Smits S,Holterman L,Damen I,Vogels R,Thorner AR,O'Brien KL,Carville A,Mansfield KG,Goudsmit J,Havenga MJ,Barouch DH

    更新日期:2007-05-01 00:00:00

  • Differential requirements of NS4A for internal NS3 cleavage and polyprotein processing of hepatitis C virus.

    abstract::The NS3 protein of hepatitis C virus (HCV) possesses protease activity responsible for the proteolytic cleavage of the viral polyprotein at the junctions of nonstructural proteins downstream of NS3. The NS3 protein was also found to be internally cleaved. In this study, we demonstrated that internal cleavages occurred...

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    pub_type: 杂志文章


    authors: Kou YH,Chang MF,Wang YM,Hung TM,Chang SC

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  • Characterization of bovine viral diarrhea virus proteins.

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    pub_type: 杂志文章


    authors: Purchio AF,Larson R,Collett MS

    更新日期:1984-05-01 00:00:00

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Messerle M,Keil GM,Koszinowski UH

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Aubert C,Brahic M

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    abstract::The small membrane glycoprotein (GS) of equine arteritis virus (EAV) is a minor virion component but is abundantly expressed in EAV-infected cells. In this study, we have analyzed its membrane topology, folding, oligomerization, and intracellular transport. We show that GS is a class I integral membrane protein with o...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: de Vries AA,Raamsman MJ,van Dijk HA,Horzinek MC,Rottier PJ

    更新日期:1995-06-01 00:00:00

  • Xenotropic murine leukemia virus-related virus establishes an efficient spreading infection and exhibits enhanced transcriptional activity in prostate carcinoma cells.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rodriguez JJ,Goff SP

    更新日期:2010-03-01 00:00:00

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    abstract::We have established a genetic assay for the multimerization of retroviral gag polyproteins. This assay is based on the GAL4 two-hybrid system for studying protein-protein interactions (S. Fields and O. Song, Nature (London) 340:245-246, 1989). In our initial experiments, we generated Saccharomyces cerevisiae plasmids ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Luban J,Alin KB,Bossolt KL,Humaran T,Goff SP

    更新日期:1992-08-01 00:00:00

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    abstract::PCR amplification of baboon endogenous virus (BaEV) long terminal repeat, reverse transcriptase gene, and env fragments from 24 different species of African monkeys indicates that BaEV is less widespread than was formerly thought. Instead of being present in every species of African primates, BaEV can be found only in...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: van der Kuyl AC,Dekker JT,Goudsmit J

    更新日期:1995-12-01 00:00:00

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    abstract::During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partia...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Junjhon J,Edwards TJ,Utaipat U,Bowman VD,Holdaway HA,Zhang W,Keelapang P,Puttikhunt C,Perera R,Chipman PR,Kasinrerk W,Malasit P,Kuhn RJ,Sittisombut N

    更新日期:2010-08-01 00:00:00

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    abstract::We have previously demonstrated that parainfluenza virus type 3 (PIV3), a significant respiratory pathogen, can markedly inhibit T-cell function in vitro. We now report that the virus potently induces interleukin-10 (IL-10) production by peripheral blood mononuclear cells. The IL-10 produced contributes to viral inhib...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Sieg S,King C,Huang Y,Kaplan D

    更新日期:1996-07-01 00:00:00

  • A genetic system for rhesus monkey rhadinovirus: use of recombinant virus to quantitate antibody-mediated neutralization.

    abstract::Rhesus monkey rhadinovirus (RRV), a simian gamma-2 herpesvirus closely related to the Kaposi sarcoma-associated herpesvirus, replicates lytically in cultured rhesus monkey fibroblasts and establishes persistence in B cells. Overlapping cosmid clones were generated that encompass the entire 130-kilobase-pair genome of ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bilello JP,Morgan JS,Damania B,Lang SM,Desrosiers RC

    更新日期:2006-02-01 00:00:00

  • Complete genome sequence of Pseudomonas aeruginosa siphophage MP1412.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bae HW,Chung IY,Sim N,Cho YH

    更新日期:2012-09-01 00:00:00

  • Glycoprotein M is an essential lytic replication protein of the murine gammaherpesvirus 68.

    abstract::All herpesviruses encode a homolog of glycoprotein M (gM), which appears to function in virion morphogenesis. Despite its conservation, gM is inessential for the lytic replication of alphaherpesviruses. In order to address the importance of gM in gammaherpesviruses, we disrupted it in the murine gammaherpesvirus 68 (M...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: May JS,Colaco S,Stevenson PG

    更新日期:2005-03-01 00:00:00