Generation of multiple replication-competent retroviruses through recombination between PreXMRV-1 and PreXMRV-2.

abstract：:We used previously characterized spleen necrosis virus-based retroviral vectors and helper cells to study the strand transfers that occur during the reverse-transcription phase of a single cycle of retroviral replication. The conditions used selected only for formation of an active provirus rather than for expression ...

journal_title：Journal of virology

authors： Jones JS,Allan RW,Temin HM

更新日期：1994-01-01 00:00:00

abstract：:The in vitro transcription of the genome segments of insect cytoplasmic polyhedrosis virus was examined for a possible mechanism of coordinate control. Initiation of the first transcription cycle, primed by preincubation in the absence of CTP, was synchronous for all 10 viral genome segments. The time required for the...

journal_title：Journal of virology

authors： Smith RE,Furuichi Y

更新日期：1982-01-01 00:00:00

abstract：:Major histocompatibility complex class I (MHC I) molecules present antigenic peptides for CD8(+) T-cell recognition. Prior to cell surface expression, proper MHC I loading is conducted by the peptide-loading complex (PLC), composed of the MHC I heavy chain (HC) and β(2)-microglobulin (β(2)m), the peptide transporter T...

journal_title：Journal of virology

authors： Halenius A,Hauka S,Dölken L,Stindt J,Reinhard H,Wiek C,Hanenberg H,Koszinowski UH,Momburg F,Hengel H

更新日期：2011-04-01 00:00:00

abstract：:Hepatitis B virus (HBV) is unusual in that its surface proteins (small [S], medium, and large [L]) are not only incorporated into the virion envelope but they also bud into empty subviral particles in great excess over virions. The morphogenesis of these subviral envelope particles remains unclear, but the S protein i...

journal_title：Journal of virology

authors： Patient R,Hourioux C,Sizaret PY,Trassard S,Sureau C,Roingeard P

更新日期：2007-04-01 00:00:00

abstract：:Respiratory syncytial virus (RSV) is a major cause of morbidity and mortality. Previous studies have suggested that T-cell responses may contribute to RSV immunopathology, which could be driven by dendritic cells (DCs). DCs are productively infected by RSV, and during RSV infections, there is an increase of DCs in the...

journal_title：Journal of virology

authors： Thornburg NJ,Shepherd B,Crowe JE Jr

更新日期：2010-12-01 00:00:00

abstract：:Previous studies have established that chlorella viruses encode K(+) channels with different structural and functional properties. In the current study, we exploit the different sensitivities of these channels to Cs(+) to determine if the membrane depolarization observed during virus infection is caused by the activit...

journal_title：Journal of virology

authors： Frohns F,Käsmann A,Kramer D,Schäfer B,Mehmel M,Kang M,Van Etten JL,Gazzarrini S,Moroni A,Thiel G

更新日期：2006-03-01 00:00:00

abstract：:A viral mRNA of the late kinetic class expressed by murine cytomegalovirus (MCMV) contains an open reading frame (ORF) whose predicted protein, designated MCK-1, has homology to beta chemokines (M. R. MacDonald, X.-Y. Li, and H. W. Virgin IV, J. Virol. 71:1671-1678, 1997). The present study analyzed further the struct...

journal_title：Journal of virology

authors： MacDonald MR,Burney MW,Resnick SB,Virgin HW IV

更新日期：1999-05-01 00:00:00

abstract：:Production of human papillomavirus type 16 (HPV-16) virus particles is totally dependent on the differentiation-dependent induction of viral L1 and L2 late gene expression. The early polyadenylation signal in HPV-16 plays a major role in the switch from the early to the late, productive stage of the viral life cycle. ...

journal_title：Journal of virology

authors： Oberg D,Fay J,Lambkin H,Schwartz S

更新日期：2005-07-01 00:00:00

abstract：:Long interspersed nuclear element 1 (LINE-1; L1) retrotransposons are the most common retroelements in mammalian genomes. Unlike individual families of endogenous retroviruses (ERVs), they have remained active throughout the mammalian radiation and are responsible for most of the retroelement movement and much genome ...

journal_title：Journal of virology

authors： Erickson IK,Cantrell MA,Scott L,Wichman HA

更新日期：2011-12-01 00:00:00

abstract：:The human papillomavirus (HPV) type 16 (HPV16) E6 protein can stimulate mechanistic target of rapamycin complex 1 (mTORC1) signaling and cap-dependent translation through activation of the PDK1 and mTORC2 kinases. Here we report that HPV18 E6 also enhances cap-dependent translation. The integrity of LXXLL and PDZ prot...

journal_title：Journal of virology

authors： Spangle JM,Ghosh-Choudhury N,Munger K

更新日期：2012-07-01 00:00:00

abstract：:Densonucleosis viruses 1 and 2 both contain the three polyamines putrescine, spermidine, and spermine in complete virus particles but not in their respective top components. The polyamines, with spermidine predominant, comprise 1.41% of the virus particle by weight, which is sufficient to neutralize 26% of the single-...

journal_title：Journal of virology

authors： Kelly DC,Elliott RM

更新日期：1977-01-01 00:00:00

abstract：:The trans-activator Tat proteins coded by human immunodeficiency virus type 1 (HIV-1) and HIV-2 appear to be similar in structure and function. However, the Tat protein of HIV-2 (Tat2) activates the HIV-1 long terminal repeat (LTR) less efficiently than Tat1 (M. Emerman, M. Guyader, L. Montagnier, D. Baltimore, and M....

journal_title：Journal of virology

authors： Elangovan B,Subramanian T,Chinnadurai G

更新日期：1992-04-01 00:00:00

abstract：:The major obstacle to human immunodeficiency type 1 virus (HIV-1) eradication is a reservoir of latently infected cells that persists despite long-term antiretroviral therapy (ART) and is maintained through cellular proliferation. Long-lived memory CD4+ T cells with high self-renewal capacity, such as central memory (...

journal_title：Journal of virology

authors： Mavigner M,Zanoni M,Tharp GK,Habib J,Mattingly CR,Lichterfeld M,Nega MT,Vanderford TH,Bosinger SE,Chahroudi A

更新日期：2019-12-12 00:00:00

abstract：:Storage of the dimeric (alphabeta) form of avian myeloblastosis virus (AMV) DNA polymerase in glycerol resulted in the release of the smaller alpha subunit, as detected by glycerol gradient sedimentation. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of enzyme stored in glycerol showed the conc...

journal_title：Journal of virology

authors： Papas TS,Marciani DJ,Samuel K,Chirikjian JG

更新日期：1976-06-01 00:00:00

abstract：:Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus termed SARS-CoV. We and others have previously shown that the replication of SARS-CoV can be suppressed by exogenously added interferon (IFN), a cytokine which is normally synthesized by cells as a reaction to virus infection. Here, we demonstra...

journal_title：Journal of virology

authors： Spiegel M,Pichlmair A,Martínez-Sobrido L,Cros J,García-Sastre A,Haller O,Weber F

更新日期：2005-02-01 00:00:00

abstract：:In search of an anti-transcriptase, antibody was raised in rabbits to partially purified, soluble NS protein present in cytoplasmic extracts of cells infected with the Indiana serotype of vesicular stomatitis (VSInd) virus. This antiserum gave specific reactions of identity by agar immunodiffusion with both cytoplasmi...

journal_title：Journal of virology

authors： Imblum RL,Wagner RR

更新日期：1975-06-01 00:00:00

abstract：:H9N2 influenza viruses are panzootic in domestic poultry in Eurasia and since 1999 have caused transient infections in humans and pigs. To investigate the zoonotic potential of H9N2 viruses, we studied the evolution of the viruses in live-poultry markets in Hong Kong in 2003. H9N2 was the most prevalent influenza viru...

journal_title：Journal of virology

authors： Choi YK,Ozaki H,Webby RJ,Webster RG,Peiris JS,Poon L,Butt C,Leung YH,Guan Y

更新日期：2004-08-01 00:00:00

abstract：:The purpose of this study was to define the relationship between herpes simplex virus (HSV) latency and in vivo ganglionic reactivation. Groups of mice with numbers of latently infected neurons ranging from 1.9 to 24% were generated by varying the input titer of wild-type HSV type 1 strain 17syn+. Reactivation of the ...

journal_title：Journal of virology

authors： Sawtell NM

更新日期：1998-08-01 00:00:00

abstract：:A tetracycline-regulated gene expression system and a panel of novel monoclonal antibodies were used to examine the subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus (HCV) NS3-NS4A complex in inducible cell lines. The NS3 serine protease domain and the full-length NS3 protein ...

journal_title：Journal of virology

authors： Wölk B,Sansonno D,Kräusslich HG,Dammacco F,Rice CM,Blum HE,Moradpour D

更新日期：2000-03-01 00:00:00

abstract：:Deletion mutants of bacteriphage T5 can be identified by analyzing the DNA content of single plaques, after alkaline denaturation, by agarose gel electrophoresis. Use of this procedure has resulted in the isolation of a new mutant, designated del2, that lacks a 1.6% segment located 68 to 70% from the left end of T5 DN...

journal_title：Journal of virology

authors： Rhoades M,Schwartz J,Wahl JM

更新日期：1980-11-01 00:00:00

abstract：:Vaccine-elicited humoral immune responses comprise an array of antibody forms and specificities, with only a fraction contributing to protective host immunity. Elucidation of antibody effector functions responsible for protective immunity against human immunodeficiency virus type 1 (HIV-1) acquisition is a major goal ...

journal_title：Journal of virology

authors： Wills S,Hwang KK,Liu P,Dennison SM,Tay MZ,Shen X,Pollara J,Lucas JT,Parks R,Rerks-Ngarm S,Pitisuttithum P,Nitayapan S,Kaewkungwal J,Thomas R,Kim JH,Michael NL,Robb ML,McRaven M,Montefiori DC,Hope TJ,Liao HX,Mood

更新日期：2018-03-14 00:00:00

abstract：:The steady-state level and metabolic half-life of retinoblastoma tumor suppressor protein pRB are decreased in cells that express high-risk human papillomavirus (HPV) E7 proteins. Here we show that pRB degradation is a direct activity of E7 and does not reflect a property of cell lines acquired during the selection pr...

journal_title：Journal of virology

authors： Gonzalez SL,Stremlau M,He X,Basile JR,Münger K

更新日期：2001-08-01 00:00:00

abstract：:A adenovirus type 5 host range mutant (hr440) has been isolated which is defective in a splicing event required to generate the middle-sized mRNA from early region 1A. This defect has been ascribed to two adjacent nucleotide changes which lie five and six nucleotides from the 5' splice site for this mRNA (Solnick, Nat...

journal_title：Journal of virology

authors： Solnick D,Anderson MA

更新日期：1982-04-01 00:00:00

abstract：:Packaging of type C retrovirus genomic RNAs into budding virions requires a highly specific interaction between the viral Gag precursor and unique cis-acting packaging signals on the full-length RNA genome, allowing the selection of this RNA species from among a pool of spliced viral RNAs and similar cellular RNAs. Th...

journal_title：Journal of virology

authors： Harrison GP,Miele G,Hunter E,Lever AM

更新日期：1998-07-01 00:00:00

abstract：UNLABELLED:Epstein-Barr virus (EBV) is a well-established B-cell-tropic virus associated with various lymphoproliferative diseases of both B-cell and non-B-cell origin. EBV is associated with a number of T-cell lymphomas; however, in vitro studies utilizing prototypical EBV type 1 (EBV-1) laboratory strains have genera...

journal_title：Journal of virology

authors： Coleman CB,Wohlford EM,Smith NA,King CA,Ritchie JA,Baresel PC,Kimura H,Rochford R

更新日期：2015-02-01 00:00:00

abstract：:Vesicular stomatitis virus (VSV) has been shown in laboratory studies to be effective against a variety of tumors, including malignant brain tumors. However, attenuation of VSV may be necessary to balance the potential toxicity toward normal cells, particularly when targeting brain tumors. Here we compared 10 recombin...

journal_title：Journal of virology

authors： Wollmann G,Rogulin V,Simon I,Rose JK,van den Pol AN

更新日期：2010-02-01 00:00:00

abstract：:Viruses are considered to use vesicular trafficking in infected cells, but the details of assembly/release pathways of hepatitis B virus (HBV) are still unknown. To identify key regulators of HBV production, we performed short interfering RNA (siRNA) screening for Rab proteins, which are considered to act as molecular...

journal_title：Journal of virology

authors： Inoue J,Ninomiya M,Umetsu T,Nakamura T,Kogure T,Kakazu E,Iwata T,Takai S,Sano A,Fukuda M,Watashi K,Isogawa M,Tanaka Y,Shimosegawa T,McNiven MA,Masamune A

更新日期：2019-07-17 00:00:00

abstract：UNLABELLED:Demonstrating direct interactions between host and virus proteins during infection is a major goal and challenge for the field of virology. Most protein interactions are not binary or easily amenable to structural determination. Using infectious preparations of a polerovirus (Potato leafroll virus [PLRV]) an...

journal_title：Journal of virology

authors： DeBlasio SL,Chavez JD,Alexander MM,Ramsey J,Eng JK,Mahoney J,Gray SM,Bruce JE,Cilia M

更新日期：2015-12-09 00:00:00

abstract：UNLABELLED:Many murine leukemia viruses (MLVs) are partially resistant to restriction by mouse APOBEC3 (mA3) and essentially fully resistant to induction of G-to-A mutations by mA3. In contrast, Vif-deficient HIV-1 (ΔVif HIV-1) is profoundly restricted by mA3, and the restriction includes high levels of G-to-A mutation...

journal_title：Journal of virology

authors： Nair S,Sanchez-Martinez S,Ji X,Rein A

更新日期：2014-04-01 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus that has been implicated in the pathogenesis of Kaposi's sarcoma. KSHV encodes K-bZIP (open reading frame K8), a protein that belongs to the basic region-leucine zipper (bZIP) family of transcription factors. Here we show that K-bZIP associates with ...

journal_title：Journal of virology

authors： Park J,Seo T,Hwang S,Lee D,Gwack Y,Choe J

更新日期：2000-12-01 00:00:00