Abstract:
:Deoxyribonucleic acid (DNA) synthesis was studied in poxvirus-infected cells by measuring (14)C-thymidine incorporation into viral and host cell DNA. A complete separation of the two species of DNA was achieved by combining the previously used "Dounce method" with a separation method based on different reannealing properties of viral and vertebrate DNA. Shortly after infection of HeLa cells with poxviruses, a burst of viral DNA synthesis occurred in the cytoplasm, but a rapid inhibition of host-cell DNA synthesis in the nucleus was observed. This inhibition of cellular DNA synthesis was also found if an accumulation of viral DNA was prevented. At high multiplicites, ultraviolet-irradiated virus inhibited host-cell DNA synthesis to the same extent as fully infectious poxvirus. Under the same conditions, heating at 60 C for 15 min caused a decrease in the ability of cowpox virus to inhibit host-cell DNA synthesis, but did not produce the same effect on vaccinia virus strain WR.
journal_name
J Viroljournal_title
Journal of virologyauthors
Jungwirth C,Launer Jdoi
10.1128/JVI.2.5.401-408.1968subject
Has Abstractpub_date
1968-05-01 00:00:00pages
401-8issue
5eissn
0022-538Xissn
1098-5514journal_volume
2pub_type
杂志文章abstract::The herpesvirus saimiri genome encodes a complement control protein homolog (CCPH). Stable mammalian cell transfectants expressing a recombinant transmembrane form of CCPH (mCCPH) or a 5'FLAG epitope-tagged mCCPH (5'FLAGmCCPH) conferred resistance to complement-mediated cell damage by inhibiting the lytic activity of ...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.7.3500-3508.1990
更新日期:1990-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3712-3720.1995
更新日期:1995-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1304-1313.1990
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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doi:10.1128/JVI.28.3.772-785.1978
更新日期:1978-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1969-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5288-5301.2001
更新日期:2001-06-01 00:00:00
abstract::Wild-type simian virus 40 A protein (large T antigen) bound to three tandem regions of simian virus 40 DNA. The binding regions were defined by the ability of A protein to protect simian virus 40 DNA from digestion with limited (footprint assay) or excess (fragment assay) amounts of DNase I. At low concentrations, pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.458-466.1982
更新日期:1982-11-01 00:00:00