Abstract:
:Kaposi's sarcoma-associated herpesvirus (KSHV) (also called human herpesvirus 8) is a novel gammaherpesvirus strongly implicated in the pathogenesis of Kaposi's sarcoma. Although virions can be produced in high yield from latently infected B-cell lines treated with phorbol esters, little is known about the infectivity of such virus, and efficient serial propagation of KSHV has been problematic. Here we report on the infectivity of KSHV produced from phorbol-induced BCBL-1 cells, employing an assay based on the detection of a spliced late mRNA by a sensitive reverse transcriptase PCR (RT-PCR) method. The results of this study confirm previous observations that 293 cells are susceptible to viral infection; however, infection with BCBL-1-derived virus is inefficient and the pattern of viral gene expression in infected cells may not fully reproduce that of authentic lytic infection. In keeping with this finding, serial propagation of BCBL-1-derived virus could not be demonstrated on 293 cells. Eleven of 38 other cell lines tested also supported KSHV infection, as judged by this RT-PCR assay, including cells of B-cell, endothelial, epithelial, and fibroblastic origin; however, in all cases, infection proceeded at or below the levels observed in 293 cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Renne R,Blackbourn D,Whitby D,Levy J,Ganem Ddoi
10.1128/JVI.72.6.5182-5188.1998subject
Has Abstractpub_date
1998-06-01 00:00:00pages
5182-8issue
6eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::Under natural conditions and in some experimental models, rabies virus infection of the central nervous system causes relatively mild histopathological changes, without prominent evidence of neuronal death despite its lethality. In this study, the effects of rabies virus infection on the structure of neurons were inve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01677-07
更新日期:2008-01-01 00:00:00
abstract::Human papillomavirus (HPV) infection of the genital tract is common; however, only about 10 to 15% of infections persist, and approximately 10 to 15% of these persistent infections result in cancer. Basal epidermal stem cells are the presumed target cells for HPV infection, providing a reservoir of latently infected c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00331-18
更新日期:2018-05-14 00:00:00
abstract::Foamy viruses (FVs) are the least known retroviruses commonly found in primates, cats, horses, and cattle. Although FVs are considered apathogenic, simian and feline FVs have been shown to be associated with some transient health abnormalities in animal models. Currently, data regarding the course of infection with bo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02447-12
更新日期:2013-03-01 00:00:00
abstract::Particular interest in human T lymphocyte lymphoma/leukemia virus (HTLV) derives from the close association of HTLV with several types of human mature T lymphocyte malignancies and the strong possibility that HTLV is the causative agent of this group of leukemias and lymphomas. This is the first report to show that HT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.440-446.1985
更新日期:1985-02-01 00:00:00
abstract::During the S phase of the eukaryotic cell cycle and in virus-infected cells, DNA replication takes place at discrete sites in the nucleus, although it is not clear how the proteins involved in the replicative process are directed to these sites. Nuclear factor I is a cellular, sequence-specific DNA-binding protein uti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.3140-3150.1992
更新日期:1992-05-01 00:00:00
abstract::Our previous studies have shown that targeting DNA vaccine-encoded major histocompatibility complex class I epitopes to the proteasome enhanced CD8(+) T-cell induction and protection against lymphocytic choriomeningitis virus (LCMV) challenge. Here, we expand these studies to evaluate CD4(+) T-cell responses induced b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10421-10430.2001
更新日期:2001-11-01 00:00:00
abstract::Infection with human papillomavirus (HPV) is a critical factor in the pathogenesis of most cervical cancers and some aerodigestive cancers. The HPV E6 oncoprotein from high-risk HPV types contributes to the immortalization and transformation of cells by multiple mechanisms, including degradation of p53, transcriptiona...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01942-05
更新日期:2006-06-01 00:00:00
abstract::Polyamine depletion produced by exogenous arginine in Escherichia coliK-12 cultures defective in agmatine ureohydrolase activity resulted in a marked inhibition of the rates of growth and nucleic acid synthesis. Addition of putrescine or spermidine to such depleted cultures restored the control rate of growth and nucl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.3.423-430.1972
更新日期:1972-03-01 00:00:00
abstract::Although iridoviruses vary widely within and among genera with respect to their host range and virulence, variation within iridovirus species has been less extensively characterized. This study explores the nature and extent of intraspecific variation within an emerging iridovirus of North American warm-water fishes, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.16.8812-8818.2003
更新日期:2003-08-01 00:00:00
abstract::The state of intracellular viral DNA in cells infected with channel catfish virus has been studied by the Hirt selective extraction procedure and by restriction endonuclease digestion. The sedimentation properties and restriction patterns of viral DNA in the Hirt supernatant fraction indicate that the majority, if not...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.405-412.1983
更新日期:1983-05-01 00:00:00
abstract::We have developed a means to identify genes associated with particular aspects of virulence. By beginning with an avirulent deletion mutant of rabbitpox virus and systematically reintroducing overlapping segments of the deleted region, we have identified two regions of the viral genome associated with increased virule...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1530-1542.1991
更新日期:1991-03-01 00:00:00
abstract::HIV circumvents HLA class I-restricted CD8+ T-cell responses through selection of escape mutations that leave characteristic mutational "footprints," also known as HLA-associated polymorphisms (HAPs), on HIV sequences at the population level. While many HLA footprints are universal across HIV subtypes and human popula...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01128-17
更新日期:2018-01-02 00:00:00
abstract::Mouse mammary tumor virus (MTV) was isolated from the milk of RIII mice by density-gradient centrifugation in Ficoll. The homogeneity of the preparation was demonstrated in electron micrographs. The nucleic acid was extracted with phenol in the presence of Pronase. Its viral origin was attested by failure of ribo-nucl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.2.230-236.1970
更新日期:1970-02-01 00:00:00
abstract::Transgenic (Tg) mice expressing full-length bovine prion protein (BoPrP) serially propagate bovine spongiform encephalopathy (BSE) prions without posing a transmission barrier. These mice also posed no transmission barrier for Suffolk sheep scrapie prions, suggesting that cattle may be highly susceptible to some sheep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5259-5271.2005
更新日期:2005-05-01 00:00:00
abstract::Reverse transcription in avian sarcoma virus (ASV) initiates from the 3' end of a tRNA(Trp) primer, which anneals near the 5' end of the RNA genome. The region around the primer-binding site (PBS) forms an elaborate stem structure composed of the U5-inverted repeat (U5-IR) stem, the U5-leader stem, and the association...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7571-7577.2002
更新日期:2002-08-01 00:00:00
abstract::The hepadnavirus reverse transcriptase is a multifunction enzyme. In addition to its role in DNA synthesis, the polymerase is required for RNA packaging and also functions as the primer for minus-strand DNA synthesis. Previously, we demonstrated that the protein-priming activity of the polymerase requires a viral RNA ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.8437-8442.1994
更新日期:1994-12-01 00:00:00
abstract::Since parvoviruses apparently do not possess a DNA polymerase activity, one or more of the host cell DNA polymerases must be responsible for replicating the single-stranded DNA genome. We have focused on determining which polymerase, alpha, beta, or gamma (pol alpha, pol beta, or pol gamma, respectively), is responsib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.652-657.1984
更新日期:1984-03-01 00:00:00
abstract::The possible addition of extra sequences to simian virus 40 (SV40) DNA was analyzed by electron microscopy in two different cell systems, productively infected monkey cells and activated heterokaryons on monkey and transformed mouse 3T3 cells. We found that the closed circular DNA fraction, extracted from monkey cells...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.2.323-337.1977
更新日期:1977-08-01 00:00:00
abstract::We determined the capacity of microcapsules formed by the combination of sodium alginate, an aqueous anionic polymer, and spermine hydrochloride, an aqueous cationic amine, to enhance protection against rotavirus challenge in mice. Adult BALB/c mice were orally inoculated with either free or microencapsulated rotaviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3859-3862.1998
更新日期:1998-05-01 00:00:00
abstract::We have examined the attachment and penetration phenotypes of several glycoprotein gIII mutants of pseudorabies virus (PRV) and have identified the first one-third of gIII as a region that mediates efficient virus attachment to PK15 and Vero cells. This portion of gIII, amino acids 25 through 157 of the wild-type sequ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2646-2654.1993
更新日期:1993-05-01 00:00:00
abstract::A recent experiment involving simian immunodeficiency virus (SIV) infection of macaques revealed that the infectivity of this virus decreased over the first few months of infection. Based on this observation, we introduce a viral dynamic model in which viral infectivity varies over time. The model is fit to viral load...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02284-09
更新日期:2010-05-01 00:00:00
abstract::Passage of native classical swine fever virus (CSFV) in cultured swine kidney cells (SK6 cells) selects virus variants that attach to the surface of cells by interaction with membrane-associated heparan sulfate (HS). A Ser-to-Arg change in the C terminus of envelope glycoprotein E(rns) (amino acid 476 in the open read...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9585-9595.2001
更新日期:2001-10-01 00:00:00
abstract::Polyoma virus particles were purified by equilibrium centrifugation in CsCl. Particles from three regions of the density gradient were examined for infectivity, for their ability to induce expanded pools of deoxyribonucleic acid (DNA) precursors, and for their ability to stimulate the synthesis of DNA. The most infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.1.28-32.1970
更新日期:1970-07-01 00:00:00
abstract::The immune response to bovine herpesvirus 1 (BHV-1) infection can protect cattle from subsequent challenge with the virus. This protection involves a variety of defensive strategies, and the activation of most of these defenses requires the recognition of viral proteins by the cellular immune system. To identify some ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.5114-5122.1990
更新日期:1990-10-01 00:00:00
abstract::In 2002, severe acute respiratory syndrome (SARS)-coronavirus (CoV) appeared as a novel human virus with high similarity to bat coronaviruses. However, while SARS-CoV uses the human angiotensin-converting enzyme 2 (ACE2) receptor for cellular entry, no coronavirus isolated from bats appears to use ACE2. Here we show t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00311-12
更新日期:2012-06-01 00:00:00
abstract::Replication and assembly of hepatitis C virus (HCV) depend on the host's secretory and lipid-biosynthetic machinery. Viral replication occurs on endoplasmic reticulum (ER)-derived modified membranes, while viral assembly is thought to occur on lipid droplets (LDs). A physical association and coordination between the v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00496-12
更新日期:2012-06-01 00:00:00
abstract::Cytomegalovirus (CMV) disease after bone marrow (BM) transplantation is often associated with BM graft failure. There are two possible reasons for such a correlation. First, a poor hematopoietic reconstitution of unrelated etiology could promote the progression of CMV infection by the lack of immune control. Alternati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.5006-5015.1998
更新日期:1998-06-01 00:00:00
abstract::The RNA produced in vivo from bacteriophage phiX174 DNA has been analyzed by polyacrylamide-agarose gel electrophoresis and sedimentation in dimethyl sulfoxide gradients, and the results of Hayashi and Hayashi (1970) have been confirmed and extended. An efficient procedure for recovery of RNA from gels, followed by a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.151-160.1975
更新日期:1975-01-01 00:00:00
abstract::Borna disease virus (BDV) is a nonsegmented negative-strand (NNS) RNA virus that is unusual because it replicates in the nucleus. The most abundant viral protein in infected cells is a 38/39-kDa doublet that is presumed to represent the nucleocapsid. Infectious particles also contain high levels of this protein, accou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5133-5139.1997
更新日期:1997-07-01 00:00:00
abstract:UNLABELLED:Macrophages must react to a large number of pathogens and their effects. In chronic HIV infection, the microenvironment changes with an influx of microbial products that trigger Toll-like receptors (TLRs). That dynamic nature can be replicated ex vivo by the proinflammatory (M1-polarized) and alternatively a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01053-14
更新日期:2014-09-01 00:00:00