Abstract:
:Transgenic (Tg) mice expressing full-length bovine prion protein (BoPrP) serially propagate bovine spongiform encephalopathy (BSE) prions without posing a transmission barrier. These mice also posed no transmission barrier for Suffolk sheep scrapie prions, suggesting that cattle may be highly susceptible to some sheep scrapie strains. Tg(BoPrP) mice were also found to be susceptible to prions from humans with variant Creutzfeldt-Jakob disease (CJD); on second passage in Tg(BoPrP) mice, the incubation times shortened by 30 to 40 days. In contrast, Tg(BoPrP) mice were not susceptible to sporadic, familial, or iatrogenic CJD prions. While the conformational stabilities of bovine-derived and Tg(BoPrP)-passaged BSE prions were similar, the stability of sheep scrapie prions was higher than that found for the BSE prions but lower if the scrapie prions were passaged in Tg(BoPrP) mice. Our findings suggest that BSE prions did not arise from a sheep scrapie strain like the one described here; rather, BSE prions may have arisen spontaneously in a cow or by passage of a scrapie strain that maintains its stability upon passage in cattle. It may be possible to distinguish BSE prions from scrapie strains in sheep by combining conformational stability studies with studies using novel Tg mice expressing a chimeric mouse-BoPrP gene. Single-amino-acid substitutions in chimeric PrP transgenes produced profound changes in incubation times that allowed us to distinguish prions causing BSE from those causing scrapie.
journal_name
J Viroljournal_title
Journal of virologyauthors
Scott MR,Peretz D,Nguyen HO,Dearmond SJ,Prusiner SBdoi
10.1128/JVI.79.9.5259-5271.2005subject
Has Abstractpub_date
2005-05-01 00:00:00pages
5259-71issue
9eissn
0022-538Xissn
1098-5514pii
79/9/5259journal_volume
79pub_type
杂志文章abstract::Chimeric bovine respiratory syncytial viruses (BRSV) expressing glycoproteins of bovine parainfluenza virus type 3 (BPIV-3) instead of BRSV glycoproteins were generated from cDNA. In the BRSV antigenome cDNA, the open reading frames of the major BRSV glycoproteins, attachment protein G and fusion protein F, were repla...
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pub_type: 杂志文章
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abstract::Canine distemper virus attachment (hemagglutinin [H] equivalent) and fusion (F) antigens were purified by affinity chromatography with monoclonal antibodies. The purified antigens were used to immunize groups of three dogs. Radioimmune precipitation assays with sera from these animals showed that the F antigen prepara...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.536-541.1986
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.1.97-109.1967
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3938-3944.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.3042-3047.1992
更新日期:1992-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02232-10
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pub_type: 杂志文章
doi:10.1128/JVI.01552-06
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00880-06
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pub_type: 杂志文章
doi:10.1128/JVI.72.6.5093-5098.1998
更新日期:1998-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02234-08
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00055-12
更新日期:2012-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01358-15
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abstract:UNLABELLED:Replication-deficient rabies virus (RABV)-based vaccines induce rapid and potent antibody responses via T cell-independent and T cell-dependent mechanisms. To further investigate early events in vaccine-induced antibody responses against RABV infections, we studied the role of macrophages as mediators of RAB...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03409-14
更新日期:2015-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02669-15
更新日期:2016-04-14 00:00:00
abstract::Cell-free translation of separated Sendai virus mRNA species identified the message for polypeptide M and suggested the identity of the message for polypeptide NP. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.411-414.1978
更新日期:1978-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00552-14
更新日期:2014-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4341-4349.2002
更新日期:2002-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.1.170-176.1977
更新日期:1977-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.66.8.5100-5105.1992
更新日期:1992-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02279-15
更新日期:2015-11-04 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.2.546-546.1979
更新日期:1979-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3175-3182.1994
更新日期:1994-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.6097-6102.1994
更新日期:1994-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01148-12
更新日期:2013-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5009-5017.2001
更新日期:2001-06-01 00:00:00
abstract::The Us11 protein of herpes simplex virus 1 (HSV-1) is an accessory factor with multiple functions. In virus-infected cells, it inhibits double-stranded RNA-dependent protein kinase (PKR), 2',5'-oligoadenylate synthetase, RIG-I, and MDA-5. However, its precise role is incompletely defined. By screening a human cDNA lib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00402-18
更新日期:2018-06-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00200-12
更新日期:2012-06-01 00:00:00