Abstract:
UNLABELLED:Many coxsackievirus B (CVB) isolates bind to human decay-accelerating factor (DAF) as well as to the coxsackievirus and adenovirus receptor (CAR). However, the virus does not interact with murine DAF. To understand why CVB3 binds specifically to human DAF, we constructed a series of chimeric molecules in which specific regions of the human DAF molecule were replaced by the corresponding murine sequences. We found that replacement of human short consensus repeat 2 (SCR2) with murine SCR2 ablated virus binding to human DAF, as did deletion of human SCR2. Although replacement of human SCR4 had a partial inhibitory effect, deletion of SCR4 had no effect. Within human SCR2, replacement of serine 104 (S104) with the proline residue found in murine DAF eliminated virus binding. On the basis of the structure of the CVB3-DAF complex determined by cryo-electron microscopy, DAF S104 is in close contact with a viral capsid residue, a threonine at VP1 position 271. Replacement of this capsid residue with larger amino acids specifically eliminated virus attachment to human DAF but had no effect on attachment to CAR or replication in HeLa cells. Taken together, these results support the current model of virus-DAF interaction and point to a specific role for VP1 T271 and DAF S104 at the virus-DAF interface. IMPORTANCE:The results of the present study point to a specific role for VP1 T271 and DAF S104 at the interface between CVB3 and DAF, and they demonstrate how subtle structural changes can dramatically influence virus-receptor interactions. In addition, the results support a recent pseudoatomic model of the CVB3-DAF interaction obtained by cryo-electron microscopy.
journal_name
J Viroljournal_title
Journal of virologyauthors
Pan J,Zhang L,Organtini LJ,Hafenstein S,Bergelson JMdoi
10.1128/JVI.02798-14subject
Has Abstractpub_date
2015-01-15 00:00:00pages
1324-8issue
2eissn
0022-538Xissn
1098-5514pii
JVI.02798-14journal_volume
89pub_type
杂志文章abstract::Hepatitis A virus (HAV), an atypical member of the Picornaviridae, grows poorly in cell culture. To define determinants of HAV growth, we introduced a blasticidin (Bsd) resistance gene into the virus genome and selected variants that grew at high concentrations of Bsd. The mutants grew fast and had increased rates of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01470-09
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abstract::The efficiency of processing of polyoma viral RNA and of its export from nucleus to cytoplasm was measured in primary mouse kidney cells by comparing the initial rates of incorporation of [3H]uridine into cytoplasmic and nuclear viral RNA. Appropriate methods of cell fractionation were chosen to maximize yields of cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.2.628-635.1981
更新日期:1981-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5939-5947.1990
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.1075-1084.1986
更新日期:1986-12-01 00:00:00
abstract::The Middle East respiratory syndrome coronavirus (MERS-CoV) nonstructural protein 16 (nsp16) is an S-adenosyl-l-methionine (SAM)-dependent 2'-O-methyltransferase (2'-O-MTase) that is thought to methylate the ribose 2'-OH of the first transcribed nucleotide (N1) of viral RNA cap structures. This 2'-O-MTase activity is ...
journal_title:Journal of virology
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abstract::Replication of the human herpesvirus Epstein-Barr virus drastically impairs cellular protein synthesis. This shutoff phenotype results from mRNA degradation upon expression of the early lytic-phase protein BGLF5. Interestingly, BGLF5 is the viral DNase, or alkaline exonuclease, homologues of which are present througho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00309-12
更新日期:2012-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01799-12
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.3.845-850.1987
更新日期:1987-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3833-3843.2006
更新日期:2006-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.876-879.1984
更新日期:1984-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6625-6634.2001
更新日期:2001-07-01 00:00:00
abstract::A series of plasmids containing hepatitis A virus (HAV) cDNA was constructed such that positive-strand HAV RNA could be transcribed with T7 RNA polymerase. The plasmids differed in the number of 5'-terminal nucleotides representing the junctions between vectors and HAV sequences that were present in the transcripts. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2757-2760.1991
更新日期:1991-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.6.938-945.1972
更新日期:1972-06-01 00:00:00
abstract:UNLABELLED:In contrast to many viruses, human cytomegalovirus (HCMV) is unable to productively infect most cancer-derived cell lines. The mechanisms of this restriction are unclear. To explore this issue, we tested whether defined oncogenic alleles, including the simian virus 40 (SV40) T antigen (TAg) and oncogenic H-R...
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pub_type: 杂志文章
doi:10.1128/JVI.00179-16
更新日期:2016-04-14 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14698-14707.2005
更新日期:2005-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.4.434-437.1971
更新日期:1971-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5505-5508.1989
更新日期:1989-12-01 00:00:00
abstract::The human immunodeficiency virus Tat protein is essential for virus replication and is a candidate vaccine antigen. Macaques immunized with Tat or chemically modified Tat toxoid having the same clade B sequence developed strong antibody responses. We compared these antisera for their abilities to recognize diverse Tat...
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.44.3.877-881.1982
更新日期:1982-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.01396-16
更新日期:2016-10-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.14.7903-7913.2003
更新日期:2003-07-01 00:00:00
abstract::The steady-state level and metabolic half-life of retinoblastoma tumor suppressor protein pRB are decreased in cells that express high-risk human papillomavirus (HPV) E7 proteins. Here we show that pRB degradation is a direct activity of E7 and does not reflect a property of cell lines acquired during the selection pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7583-7591.2001
更新日期:2001-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.7.3058-3066.2000
更新日期:2000-04-01 00:00:00
abstract::The structure of Bacillus subtilis bacteriophage phi25 and phi25 deoxyribonucleic acid (DNA) were studied by electron microscopy. The head of phi25 is a regular polyhedron measuring 75 nm in diameter. The uncontracted tail of phi25 is 130 nm in length and includes a large, complex tail plate. Phage phi25 DNA is double...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.1.107-113.1970
更新日期:1970-07-01 00:00:00
abstract::The lytic switch transactivator Zta initiates the ordered cascade of Epstein-Barr virus gene expression that culminates in virus production. Zta is a sequence-specific DNA-binding protein that transactivates early viral promotes via cis-acting sequences. Activation of some of these genes is mediated through binding to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4206-4212.1995
更新日期:1995-07-01 00:00:00
abstract::The development of resistance to host defense mechanisms such as tumor necrosis factor (TNF)- and Fas-mediated apoptosis of transformed or virus-infected cells may be a critical component in the development of disease. To find genes that protect cells from apoptosis, we used an expression cloning strategy and identifi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7509-7517.1997
更新日期:1997-10-01 00:00:00
abstract::The epithelium plays a key role in the spread of Lassa virus. Transmission from rodents to humans occurs mainly via inhalation or ingestion of droplets, dust, or food contaminated with rodent urine. Here, we investigated Lassa virus infection in cultured epithelial cells and subsequent release of progeny viruses. We s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02240-09
更新日期:2010-04-01 00:00:00
abstract:UNLABELLED:Development of a vaccine to prevent congenital cytomegalovirus infection is a major public health priority. Live vaccines attenuated through mutations targeting viral mechanisms responsible for evasion of host defense may be both safe and efficacious. Safety and vaccine efficacy were evaluated using a guinea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01419-15
更新日期:2015-10-01 00:00:00
abstract::HVEM (for herpesvirus entry mediator) is a member of the tumor necrosis factor receptor superfamily and mediates entry of many strains of herpes simplex virus (HSV) into normally nonpermissive Chinese hamster ovary (CHO) cells. We used sucrose density centrifugation to demonstrate that purified HSV-1 KOS virions bind ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3595-3601.1998
更新日期:1998-05-01 00:00:00