Abstract:
:It is possible to oxidize in situ the 3'-terminals of the double-stranded ribonucleic acid (RNA) contained in structurally intact cytoplasmic polyhedrosis virus. The number of 3'-terminals subsequently found by reduction with tritiated sodium borohydride was equivalent to the number observed in RNA first isolated from the virus and then oxidized and reduced. The viral RNA appears to occur in 10 distinct polynucleotide segments present in equimolar amounts.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lewandowski LJ,Millward Sdoi
10.1128/JVI.7.4.434-437.1971subject
Has Abstractpub_date
1971-04-01 00:00:00pages
434-7issue
4eissn
0022-538Xissn
1098-5514journal_volume
7pub_type
杂志文章abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) latently infects KS tumors, primary effusion lymphomas (PELs), and PEL cell lines. K12 (T0.7) is the most abundant transcript expressed in latent KSHV infection. We characterize here the K12 transcript from a PEL tumor prior to passage in cell culture. The PEL tumor KSHV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.11880-11888.2002
更新日期:2002-12-01 00:00:00
abstract::We measured the quantity of plasma feline immunodeficiency virus (FIV) RNA using a real-time sequence detecting system. Plasma viral RNA load was shown to correlate with the clinical stage, survival time, and disease progression in naturally FIV-infected cats. The present study indicates that the plasma viral RNA load...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.10079-10083.2002
更新日期:2002-10-01 00:00:00
abstract::The affinity of human immunodeficiency virus (HIV) envelope for CD4 and CCR5 appears to be associated with aspects of R5 virus (virus using the CCR5 coreceptor) pathogenicity. However, entry efficiency results from complex interactions between the viral envelope glycoprotein and both CD4 and CCR5, which limits attempt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01242-09
更新日期:2009-11-01 00:00:00
abstract::The kinetics of retroviral DNA and RNA synthesis are parameters vital to understanding viral growth, especially for human immunodeficiency virus (HIV), which encodes several of its own regulatory genes. We have established a single-cycle growth condition for HIV in H9 cells, a human CD4+ lymphocyte line. The full-leng...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3708-3713.1989
更新日期:1989-09-01 00:00:00
abstract::The rescue of influenza viruses by reverse genetics has been described only for the influenza A and B viruses. Based on a similar approach, we developed a reverse-genetics system that allows the production of influenza C viruses entirely from cloned cDNA. The complete sequences of the 3' and 5' noncoding regions of ty...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00910-07
更新日期:2007-10-01 00:00:00
abstract::Changes in the envelope glycoprotein ectodomains of a nonpathogenic simian-human immunodeficiency virus (SHIV-89.6) that was serially passaged in vivo have been shown to be responsible for the increased pathogenicity of the resulting virus, SHIV-KB9 (G. B. Karlsson, et al., J. Exp. Med. 188:1159-1171, 1998). The 12 am...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4433-4440.2000
更新日期:2000-05-01 00:00:00
abstract::We investigated the use of infectious cDNA for the production of poliovirus type 1-type 3 recombinants. One such recombinant virus was produced, but a second construct involving the transfer of part of the capsid protein region was not infectious. Our results suggest that the approach may prove valuable but that not a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.1187-1190.1986
更新日期:1986-03-01 00:00:00
abstract::Recent studies have explored the chromatin structures associated with the herpes simplex virus type 1 (HSV-1) genome during latency, particularly with regard to specific histone tail modifications such as acetylation and dimethylation. The objective of our present study was to develop a rapid systemic method of in viv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00070-07
更新日期:2007-06-01 00:00:00
abstract::The DA strain and other members of the TO subgroup of Theiler's murine encephalomyelitis virus (TMEV) induce an early transient subclinical neuronal disease followed by a chronic progressive inflammatory demyelination, with persistence of the virus in the central nervous system (CNS) for the life of the mouse. Althoug...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00178-11
更新日期:2011-09-01 00:00:00
abstract::Titers of vaccinia virus consistently increased in cultures of washed phytohemagglutinin-treated, peripheral blood leukocytes of a vaccinated adult. Concomitantly, a gradual rise occurred in the numbers of infected leukocytes, as determined by the infective center assay. Increase in viral titer was accompanied by cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.8.787-792.1968
更新日期:1968-08-01 00:00:00
abstract::Relative to wild-type herpes simplex virus type 1 (HSV-1), ICP0-null mutant viruses reactivate inefficiently from explanted, latently infected mouse trigeminal ganglia (TG), indicating that ICP0 is not essential for reactivation but plays a central role in enhancing the efficiency of reactivation. The validity of thes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3240-3249.2001
更新日期:2001-04-01 00:00:00
abstract::In this report, we describe the isolation, molecular genetic mapping, and phenotypic characterization of vaccinia virus mutants resistant to cytosine arabinoside (araC) and phosphonoacetic acid (PAA). At 37 degrees C, 8 microM araC was found to prevent macroscopic plaque formation by wild-type virus and to cause a 10(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4323-4336.1993
更新日期:1993-07-01 00:00:00
abstract::Neurotropic coronavirus induces acute encephalomyelitis and demyelination in mice. Infection of BALB/c (H-2(d)) mice expressing a dominant negative gamma interferon (IFN-gamma) receptor specifically in oligodendrocytes was examined to determine the influence of IFN-gamma signaling on pathogenesis. Inhibition of IFN-ga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02373-09
更新日期:2010-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) infection leads to lifelong viral persistence through its latency in B cells. EBV-specific T cells control reactivations and prevent the development of EBV-associated malignancies in most healthy carriers, but infection can sometimes cause chronic disease and malignant transformation. Epstein-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00852-13
更新日期:2013-08-01 00:00:00
abstract::Further advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations requi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.24.13819-13828.2004
更新日期:2004-12-01 00:00:00
abstract::Aluminum (Al)-based salts are widely used adjuvants in ruminants and other species to strengthen the immune response elicited against vaccine antigen(s). However, they can lead to the formation of long-lasting granulomas composed of abundant activated macrophages. Small ruminant lentiviruses (SRLV) are widely distribu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01859-20
更新日期:2020-12-22 00:00:00
abstract::We isolated revertants of a polyomavirus whose origin of DNA replication contains a point mutation in the palindrome to which large T antigen binds. Four independent second-site revertants contain an Asp-286----Asn-286 substitution in large T antigen. This mutant large T antigen activates replication of DNAs containin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.242-249.1989
更新日期:1989-01-01 00:00:00
abstract::Mice that express the viral oncoprotein simian virus 40 (SV40) large T antigen (T-Ag) as a transgene provide useful models for the assessment of the state of the host immune response in the face of spontaneous tumor progression. Line SV11 (H2(b)) mice develop rapidly progressing choroid plexus tumors due to expression...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1751-1762.2004
更新日期:2004-02-01 00:00:00
abstract::Moloney murine leukemia virus (M-MuLV) and M-MuLV-derived retroviral vectors are not expressed in early mouse embryos or in embryonal carcinoma cells. M-MuLV-derived mutants or M-MuLV-related variants which transduce the neomycin phosphotransferase gene can, however, induce drug resistance in embryonal carcinoma cells...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2742-2746.1987
更新日期:1987-09-01 00:00:00
abstract::Accurate and sensitive quantitation of infectious human immunodeficiency virus (HIV) has been difficult to achieve. In this report, a quantitative focal immunoassay (FIA) for HIV was developed using human HeLa cells rendered susceptible to HIV infection by introduction of the CD4 gene via a retrovirus vector. Infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3779-3788.1988
更新日期:1988-10-01 00:00:00
abstract::A hallmark of infection with herpes simplex virus type 1 (HSV-1) is the establishment of latency in ganglia of the infected individual. During the life of the latently infected individual, the virus can occasionally reactivate, travel back to the eye, and cause recurrent disease. Indeed, a major cause of corneal scarr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02234-08
更新日期:2009-03-01 00:00:00
abstract::The processes that facilitate transport of integral membrane proteins though the secretory pathway and subsequently target them to particular cellular membranes are relevant to almost every field of biology. These transport processes involve integration of proteins into the membrane of the endoplasmic reticulum (ER), ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.19.12528-12535.2005
更新日期:2005-10-01 00:00:00
abstract::Viral infectivity factor (Vif) is required for lentivirus fitness and pathogenicity, except in equine infectious anemia virus (EIAV). Vif enhances viral infectivity by a Cullin5-Elongin B/C E3 complex to inactivate the host restriction factor APOBEC3. Core-binding factor subunit beta (CBF-β) is a cell factor that was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01924-14
更新日期:2014-10-01 00:00:00
abstract::Antibodies directed to Sindbis virus (SV) envelope protein E2 are able to control virus replication in vivo and in persistently infected cultures of neurons in vitro. We investigated the mechanisms by which anti-E2 monoclonal antibody (MAb) alters virus replication by using AT3 rat prostatic carcinoma cells expressing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7006-7014.1995
更新日期:1995-11-01 00:00:00
abstract::Levels of the p53 tumor suppressor protein are increased in human cytomegalovirus (HCMV)-infected cells and may be important for HCMV pathogenesis. In normal cells p53 levels are kept low due to an autoregulatory feedback loop where p53 activates the transcription of mdm2 and mdm2 binds and ubiquitinates p53, targetin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3833-3843.2006
更新日期:2006-04-01 00:00:00
abstract::Current knowledge of human immunodeficiency virus type 1 envelope (Env) glycoprotein structure and function is based on studies of clade B viruses. We present evidence of sequence and structural differences in viral glycoprotein gp120 between clades B and C. In clade C, the C3 region alpha2-helix exhibits high sequenc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01954-06
更新日期:2007-05-01 00:00:00
abstract:UNLABELLED:Tetherin (BST2, CD317, or HM1.24) is a host cellular restriction factor that prevents the release of enveloped viruses by mechanically linking virions to the plasma membrane. The precise arrangement of tetherin molecules at the plasma membrane site of HIV-1 assembly, budding, and restriction is not well unde...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01880-15
更新日期:2015-11-18 00:00:00
abstract::The nucleotide sequence of the region of human polyoma virus JC DNA between 0.5 and 0.7 map units from a unique EcoRI cleavage site was determined and compared with those of the corresponding regions of another human polyoma virus, BK, and simian virus 40 DNAs. Within this region consisting of 945 base pairs, we locat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.1.73-79.1983
更新日期:1983-01-01 00:00:00
abstract:UNLABELLED:We have shown that glycoprotein K (gK) and its interacting partner, the UL20 protein, play crucial roles in virion envelopment. Specifically, virions lacking either gK or UL20 fail to acquire an envelope, thus causing accumulation of capsids in the cytoplasm of infected cells. The herpes simplex virus 1 (HSV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00278-14
更新日期:2014-06-01 00:00:00
abstract::The herpes simplex virus genome consists of two components, L and S, that invert relative to each other to yield four isomeric arrangements, prototype (P), inversion of the S component (Is), inversion of the L component (Il), and inversion of both components (Isl). Previous studies have shown that the 500-base-pair a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.583-591.1986
更新日期:1986-05-01 00:00:00