Abstract:
:The development of resistance to host defense mechanisms such as tumor necrosis factor (TNF)- and Fas-mediated apoptosis of transformed or virus-infected cells may be a critical component in the development of disease. To find genes that protect cells from apoptosis, we used an expression cloning strategy and identified BHRF1, an Epstein-Barr virus (EBV) early-lytic-cycle protein with distant homology to Bcl-2, as an anti-apoptosis protein. Expression of BHRF1 in MCF-Fas cells conferred nearly complete resistance against both anti-Fas antibody and TNF-mediated apoptosis. In addition, BHRF1 protected these cells from monocyte-mediated killing but failed to protect them from killing mediated by lymphokine-activated killer cells. The ability of BHRF1 to protect MCF-Fas cells from apoptosis induced by various stimuli was identical to that of Bcl-2 and Bcl-xL. Moreover, the mechanism of action of BHRF1 resembled that of Bcl-2 and Bcl-xL as it inhibited TNF- and anti-Fas-induced activation of two enzymes participating in the apoptosis pathway, cytosolic phospholipase A2 and caspase-3/CPP32, but did not interfere with the activation of NF-kappaB-like transcription factors. A putative function of BHRF1 in EBV-infected epithelial cells may be to protect virus-infected cells from TNF- and/or anti-Fas-induced cell death in order to maximize virus production. Surprisingly, expression of neither BHRF1 nor Bcl-2 in a B-cell line, BJAB, protected the cells from anti-Fas-mediated apoptosis even though they increased the survival of serum-starved cells. Thus, the protective role of BHRF1 against apoptosis resembles that of Bcl-2 in being cell type specific and dependent on the apoptotic stimulus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Foghsgaard L,Jäättelä Mdoi
10.1128/JVI.71.10.7509-7517.1997subject
Has Abstractpub_date
1997-10-01 00:00:00pages
7509-17issue
10eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::Propagation of the agents responsible for transmissible spongiform encephalopathies (TSEs) in cultured cells has been achieved for only a few cell lines. To establish efficient and versatile models for transmission, we developed neuroblastoma cell lines overexpressing type A mouse prion protein, MoPrP(C)-A, and then t...
journal_title:Journal of virology
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.03052-14
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.243-248.1978
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pub_type: 杂志文章
doi:10.1128/JVI.01350-20
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00533-13
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01945-14
更新日期:2014-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.9.5073-5083.2003
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.2.275-287.1978
更新日期:1978-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,评审
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9507-9514.2000
更新日期:2000-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.6.2665-2674.2001
更新日期:2001-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5473-5480.1997
更新日期:1997-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.8085-8093.2004
更新日期:2004-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.705-713.1989
更新日期:1989-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3718-3728.1988
更新日期:1988-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6450-6454.1996
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更新日期:2007-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.10.1076-1080.1968
更新日期:1968-10-01 00:00:00
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journal_title:Journal of virology
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更新日期:2015-11-11 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4423-4430.2003
更新日期:2003-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7703-7709.1999
更新日期:1999-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.1.27-33.1975
更新日期:1975-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.3.677-687.1978
更新日期:1978-09-01 00:00:00