Abstract:
:The lytic switch transactivator Zta initiates the ordered cascade of Epstein-Barr virus gene expression that culminates in virus production. Zta is a sequence-specific DNA-binding protein that transactivates early viral promotes via cis-acting sequences. Activation of some of these genes is mediated through binding to consensus AP-1 promoter elements. This observation suggests that Zta may also regulate the expression of cellular genes. While many targets of Zta have been identified in the Epstein-Barr virus genome, putative host cell targets remain largely unknown. To address this issue, a tetracycline-regulated Zta expression system was generated, and differential hybridization screening was used to isolate Zta-responsive cellular genes. The major target identified by this analysis is a gene encoding a fasciclin-like secreted factor, transforming growth factor beta igh3 (TGF-beta igh3), that was originally identified as a gene that is responsive to the potent immunosuppressor TGF-beta 1. Northern (RNA) blot analysis demonstrated that induction of Zta expression results in a 10-fold increase in TGF-beta igh3 mRNA levels. Zta was also found to increase TGF-beta 1 mRNA levels as well as the amount of active TGF-beta 1 secreted into the medium. Interestingly, alpha 1-collagen IV, which has been shown to potentiate the effects of TGF-beta 1, is also a cellular target of Zta. These results suggest that Zta could play a role in modulating the host cell environment through activating the expression of secreted factors.
journal_name
J Viroljournal_title
Journal of virologyauthors
Cayrol C,Flemington EKdoi
10.1128/JVI.69.7.4206-4212.1995subject
Has Abstractpub_date
1995-07-01 00:00:00pages
4206-12issue
7eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::In order to clarify the effect of an accumulation of amino acid substitutions on the hemadsorption character of the influenza AH3 virus hemagglutinin (HA) protein, we introduced single-point amino acid changes into the HA1 domain of the HA proteins of influenza viruses isolated in 1968 (A/Aichi/2/68) and 1997 (A/Sydne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6472-6477.2005
更新日期:2005-05-01 00:00:00
abstract::Our previous studies have argued persuasively that in murine sarcoma virus ts110 (MuSVts110) the gag and mos genes are fused out of frame due to a approximately 1.5-kilobase (kb) deletion of wild-type murine sarcoma virus 349 (MuSV-349) viral information. As a consequence of this deletion, infected cells grown at 39 d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.624-633.1985
更新日期:1985-02-01 00:00:00
abstract::In gene therapy, the innate immune system is a significant barrier to the effective application of adenovirus (Ad) vectors. In kidney epithelium-derived (REC) cells, serotype 5 Ad vectors induce the expression of the chemokine CXCL10 (IP-10), a response that is dependent on NFkappaB. Compared to the parental vector Ad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14507-14515.2005
更新日期:2005-12-01 00:00:00
abstract::Senecavirus A (SVA) is a picornavirus that causes acute vesicular disease (VD), that is clinically indistinguishable from foot-and-mouth disease (FMD), in pigs. Notably, SVA RNA has been detected in lymphoid tissues of infected animals several weeks following resolution of the clinical disease, suggesting that the vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00819-19
更新日期:2019-10-15 00:00:00
abstract::Three late transcripts have been mapped to the vaccinia virus 7.1-kilobase (kb) EcoRI F fragment, which is located approximately 85 kb from the left end of the viral genome. Hybrid selection and translation of RNA have demonstrated that these mRNAs encode three polypeptides with apparent molecular weights of 32,000 (3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.510-520.1984
更新日期:1984-02-01 00:00:00
abstract::Published work (D. J. McGeoch, Nucleic Acids Res. 18:4105-4110, 1990; J. E. McGeehan, N. W. Depledge, and D. J. McGeoch, Curr. Protein Peptide Sci. 2:325-333, 2001) has indicated that evolution of dUTPase in the class of herpesviruses that infect mammals and birds involved capture of a host gene followed by a duplicat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12880-12892.2005
更新日期:2005-10-01 00:00:00
abstract::Modification of proteins by ubiquitin is essential for numerous cellular processes. The RING-H2 finger motif has been implicated in ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Four proteins, WSSV199, WSSV222, WSSV249, and WSSV403, from white spot syndrome virus (WSSV) contain the RING-H2 motif. Here we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.8764-8772.2005
更新日期:2005-07-01 00:00:00
abstract::Abelson murine leukemia virus (Ab-MLV) encodes the v-Abl protein tyrosine kinase and induces transformation of immortalized fibroblast lines and pre-B cells. Temperature-sensitive mutations affecting the kinase domain of the protein have demonstrated that the kinase activity is absolutely required for transformation. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.4.1816-1823.2001
更新日期:2001-02-01 00:00:00
abstract::Immature retroviral particles are assembled by self-association of the structural polyprotein precursor Gag. During maturation the Gag polyprotein is proteolytically cleaved, yielding mature structural proteins, matrix (MA), capsid (CA), and nucleocapsid (NC), that reassemble into a mature viral particle. Proteolytic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05564-11
更新日期:2012-02-01 00:00:00
abstract::The distantly related lentiviruses human immunodeficiency virus type 1 (HIV-1) and visna virus each encode a posttranscriptional regulatory protein, termed Rev, that is critical for expression of the viral structural proteins. We genetically mapped the cis-acting target sequence for visna virus Rev, the visna virus Re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3609-3615.1992
更新日期:1992-06-01 00:00:00
abstract::Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00601-08
更新日期:2008-07-01 00:00:00
abstract::Recruitment and extravasation of T cells through the blood-brain barrier are favored by adhesion molecule-mediated interactions of circulating T cells with endothelial cells. Since a common pathological finding in human T-cell leukemia virus type 1 (HTLV-1)-associated diseases is the infiltration of HTLV-1-infected T ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8522-8530.1997
更新日期:1997-11-01 00:00:00
abstract::In response to viral infection, host cells elicit a number of responses, including the expression of alpha/beta interferon (IFN-alpha/beta). In these cells, IFN regulatory factor-3 (IRF-3) undergoes a sequence of posttranslational modifications that allow it to act as a potent transcriptional coactivator of specific I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8411-8420.2004
更新日期:2004-08-01 00:00:00
abstract::We characterized a number of important features of the structure of the cohesive overlap region of the DNA genome of duck hepatitis B virus. The 5'-terminal nucleotide of minus-strand DNA was localized to nucleotide 2537, a G residue within the 12-base repeat sequence DR1. This G residue was shown to be the site of a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3832-3840.1987
更新日期:1987-12-01 00:00:00
abstract::Feline panleukopenia virus (FPV) and its host range variant, canine parvovirus (CPV), can bind the feline transferrin receptor (TfR), while only CPV binds to the canine TfR. Introducing two CPV-specific changes into FPV (at VP2 residues 93 and 323) endowed that virus with the canine TfR binding property and allowed ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.10099-10105.2003
更新日期:2003-09-01 00:00:00
abstract::The largest tegument protein of herpes simplex virus 1 (HSV-1), UL36, contains a novel deubiquitinating activity embedded in it. All members of the Herpesviridae contain a homologue of HSV-1 UL36, the N-terminal segments of which show perfect conservation of those residues implicated in catalysis. For murine cytomegal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15582-15585.2005
更新日期:2005-12-01 00:00:00
abstract::The feline homolog of the alpha-chemokine receptor CXCR4 has recently been shown to support cell-cell fusion mediated by CXCR4-dependent strains of human immunodeficiency virus (HIV) and strains of feline immunodeficiency virus (FIV) that have been selected for growth in the Crandell feline kidney (CrFK) cell line. In...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6475-6481.1998
更新日期:1998-08-01 00:00:00
abstract::Complement, part of the innate immune system, acts to remove pathogens and unwanted host material. Complement is known to function in all tissues, including the central nervous system (CNS). In this study, we demonstrated the importance of the complement system within the CNS in the development of behavioral seizures ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00422-10
更新日期:2010-07-01 00:00:00
abstract::It has previously been shown that an electrophoretic variant form of the Rous sarcoma virus transforming protein, pp60v-src, exists in src-transformed cells. This variant, which was readily observed in vanadate-treated cells, was characterized as possessing extensive amino-terminal domain phosphotyrosine modification....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1593-1601.1987
更新日期:1987-05-01 00:00:00
abstract::Immunological comparisons were made of baculovirus structural proteins by using a modification of the radioimmunological techniques described by Renart et al. (Proc. Natl. Acad. Sci. U.S.A. 76: 3116-3120, 1979) and Towbin et al. (Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354, 1979). Viral proteins were electrophoresed i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.125-137.1981
更新日期:1981-07-01 00:00:00
abstract::Glycoprotein H (gH) is conserved among all herpesviruses and is essential for virus entry and cell fusion along with gL, gB, and, in most alphaherpesviruses, gD. Within the gH/gL heterodimer, it is thought that gH accounts for the fusion function and gL acts as a chaperone for the folding and transport of gH. Here, we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00097-07
更新日期:2007-05-01 00:00:00
abstract::pp60v-src is a nonreceptor protein tyrosine kinase that can transform both chicken and rodent fibroblasts. The src homology 2 (SH2) domain of this protein serves a critical role in the regulation of protein tyrosine kinase activity. The host range proteins pp60v-src-L, which contains a deletion of a highly conserved r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7267-7274.1994
更新日期:1994-11-01 00:00:00
abstract::Intracellular mature vaccinia virions are wrapped by cisternae, derived from virus-modified trans-Golgi or endosomal membranes, and then transported via microtubules to the cell periphery. Two viral proteins, encoded by the F13L and B5R open reading frames, are essential for the membrane-wrapping step. Previous transf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7777-7789.2002
更新日期:2002-08-01 00:00:00
abstract::Domains required late in the virus budding process (L domains) have been identified in the Gag proteins of a number of retroviruses. Here we show that the human T-cell leukemia virus type 1 candidate L domain motif PPPY is indeed required for virus production. Strikingly, however, mutation of this motif arrested virus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.10024-10029.2002
更新日期:2002-10-01 00:00:00
abstract::Integrated retroviruses or retroviral vectors may be transcriptionally inactive although their promoter-enhancer regions are able to direct transcription in the host cell. We have used single-gene retroviral vectors with a long terminal repeat-directed neo marker gene to determine if the level of transcription relates...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5596-5601.1994
更新日期:1994-09-01 00:00:00
abstract::Polypeptides synthesized in Newcastle disease virus (NDV)-infected CHO cells in the absence of glycosylation were characterized. Incorporation of either [3H]mannose of [3H]glucosamine into NDV polypeptides was inhibited to greater than 99% by the antibiotic tunicamycin. Under these conditions, infected cells synthesiz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.1.171-180.1980
更新日期:1980-10-01 00:00:00
abstract::We used the mammalian reoviruses to determine the molecular basis of the clearance of a virus from the bloodstream by specific organs. Reovirus serotypes 1 (T1) and 3 (T3) were radiolabeled with [35S]methionine or 125I, and the viruses were injected intravenously into weanling rats. The distribution of radioactivity w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.545-551.1988
更新日期:1988-02-01 00:00:00
abstract::The core promoter mutants of hepatitis B virus (HBV) emerge as the dominant viral population at the late HBeAg and the anti-HBe stages of HBV infection, with the A1762T/G1764A substitutions as the hotspot mutations. The double core promoter mutations were found by many investigators to moderately enhance viral genome ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.12.6601-6612.2003
更新日期:2003-06-01 00:00:00
abstract::Human cytomegalovirus (HCMV) UL84 is a phosphoprotein that shuttles from the nucleus to the cytoplasm and is required for oriLyt-dependent DNA replication and viral growth. UL84 was previously shown to interact with IE2 (IE86) in infected cells, and this interaction down-regulates IE2-mediated transcriptional activati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01559-07
更新日期:2008-01-01 00:00:00
abstract::Infectious pancreatic necrosis virus of fish, infectious bursal disease virus of chickens, Tellina virus and oyster virus of bivalve molluscs, and drosophila X virus of Drosophila melanogaster are naked icosahedral viruses with an electron microscopic diameter of 58 to 60 nm. The genome of each of these viruses consis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.2.593-605.1979
更新日期:1979-11-01 00:00:00