Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes.

abstract：:Prior immunity to influenza A virus (IAV) in mice changes the outcome to a subsequent lymphocytic choriomeningitis virus (LCMV) infection and can result in severe lung pathology, similar to that observed in patients that died of the 1918 H1N1 pandemic. This pathology is induced by IAV-specific memory CD8(+) T cells cr...

journal_title：Journal of virology

authors： Kraft AR,Wlodarczyk MF,Kenney LL,Selin LK

更新日期：2013-12-01 00:00:00

abstract：:BALB/c JLS V9 cells recently infected with Harvey sarcoma virus-murine leukemia virus (HSV-MuLV) complex contained unintegrated HSV linear DNA of 6.0-kilobase pair mass. The cells also contained two HSV closed circular DNA species along with MuLV-encoded linear and closed circular DNA species. HSV 6.0-kilobase pair li...

journal_title：Journal of virology

authors： Goldfarb MP,Weinberg RA

更新日期：1979-10-01 00:00:00

abstract：:Plasma from a small subset of subjects chronically infected with HIV-1 shows remarkable magnitude and breadth of neutralizing activity. From one of these individuals (CH0219), we isolated two broadly neutralizing antibodies (bnAbs), CH01 and VRC-CH31, from two clonal lineages of memory B cells with distinct specificit...

journal_title：Journal of virology

authors： Bonsignori M,Montefiori DC,Wu X,Chen X,Hwang KK,Tsao CY,Kozink DM,Parks RJ,Tomaras GD,Crump JA,Kapiga SH,Sam NE,Kwong PD,Kepler TB,Liao HX,Mascola JR,Haynes BF

更新日期：2012-04-01 00:00:00

abstract：:To investigate the process of human immunodeficiency virus type 1 (HIV-1) evolution in vivo, a total of 179 HIV-1 V3 sequences derived from cell-free plasma were determined from serial samples in three epidemiologically linked individuals (one infected blood donor and two transfusion recipients) over a maximum period ...

journal_title：Journal of virology

authors： Zhang L,Diaz RS,Ho DD,Mosley JW,Busch MP,Mayer A

更新日期：1997-03-01 00:00:00

abstract：:A series of plasmids containing hepatitis A virus (HAV) cDNA was constructed such that positive-strand HAV RNA could be transcribed with T7 RNA polymerase. The plasmids differed in the number of 5'-terminal nucleotides representing the junctions between vectors and HAV sequences that were present in the transcripts. W...

journal_title：Journal of virology

authors： Harmon SA,Richards OC,Summers DF,Ehrenfeld E

更新日期：1991-05-01 00:00:00

abstract：:Semlike forest virus capsid protein cosedimented with the large ribosomal subunit at 60S in sucrose gradients after treatment of cytoplasm from infected cells with Triton X-100 and EDTA. In CsCl gradients the capsid protein banded with the subunit at a density of 1.56 to 1.57 g/cm3. Most of the capsid protein could be...

journal_title：Journal of virology

authors： Ulmanen I,Söderlund H,Kääriäinen L

更新日期：1976-10-01 00:00:00

abstract：:Airborne viruses are expected to be ubiquitous in the atmosphere but they still remain poorly understood. This study investigated the temporal and spatial dynamics of airborne viruses and their genotypic characteristics in air samples collected from three distinct land use types (a residential district [RD], a forest ...

journal_title：Journal of virology

authors： Whon TW,Kim MS,Roh SW,Shin NR,Lee HW,Bae JW

更新日期：2012-08-01 00:00:00

abstract：:Rotavirus nonstructural protein 4 (NSP4) induces dramatic changes in cellular calcium homeostasis. These include increased endoplasmic reticulum (ER) permeability, resulting in decreased ER calcium stores and activation of plasma membrane (PM) calcium influx channels, ultimately causing a 2- to 4-fold elevation in cyt...

journal_title：Journal of virology

authors： Hyser JM,Utama B,Crawford SE,Broughman JR,Estes MK

更新日期：2013-12-01 00:00:00

abstract：:The cellular requirements for activation of herpesvirus fusion and entry remain poorly understood. Low pH triggers change in the antigenic reactivity of the prefusion form of the herpes simplex virus (HSV) fusion protein gB in virions, both in vitro and during viral entry via endocytosis (S. Dollery et al., J. Virol. ...

journal_title：Journal of virology

authors： Dollery SJ,Wright CC,Johnson DC,Nicola AV

更新日期：2011-10-01 00:00:00

abstract：:Vertebrates mount a strong innate immune response against viruses, largely by activating the interferon system. Double-stranded RNA (dsRNA), a common intermediate formed during the life cycle of many viruses, is a potent trigger of this response. In contrast, no general inducible antiviral defense mechanism has been r...

journal_title：Journal of virology

authors： Robalino J,Browdy CL,Prior S,Metz A,Parnell P,Gross P,Warr G

更新日期：2004-10-01 00:00:00

abstract：:Restriction factors are intrinsic cellular defense proteins that have evolved to block microbial infections. Retroviruses such as HIV-1 are restricted by TRIM5 proteins, which recognize the viral capsid shell that surrounds, organizes, and protects the viral genome. TRIM5α uses a SPRY domain to bind capsids with low i...

journal_title：Journal of virology

authors： Wagner JM,Christensen DE,Bhattacharya A,Dawidziak DM,Roganowicz MD,Wan Y,Pumroy RA,Demeler B,Ivanov DN,Ganser-Pornillos BK,Sundquist WI,Pornillos O

更新日期：2018-01-30 00:00:00

abstract：:The genomic RNA of human T-cell leukemia virus type 1 encodes three polyproteins, Gag, Gag-Pro, and Gag-Pro-Pol, which are translated as a result of no, one, and two frameshifts, respectively. In this report we demonstrate that the 77 residues encoded at the C terminus of the Gag-Pro precursor can be collectively dete...

journal_title：Journal of virology

authors： Heidecker G,Hill S,Lloyd PA,Derse D

更新日期：2002-12-01 00:00:00

abstract：:The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic response without functional Bax, a downstream tar...

journal_title：Journal of virology

authors： McLean JE,Datan E,Matassov D,Zakeri ZF

更新日期：2009-08-01 00:00:00

abstract：:Upon inoculation into AKR mice, mink cell focus-forming murine leukemia virus (MCF MLV) accelerates thymic lymphoma formation. During the preleukemic phase of disease, we observed the induction of apoptosis in thymic lymphocytes. A similar induction of apoptosis was observed for cultured mink epithelial cells after MC...

journal_title：Journal of virology

authors： Nanua S,Yoshimura FK

更新日期：2004-05-01 00:00:00

abstract：:The Parvovirus H-1 replicates autonomously in hamster embryo cells. A DNA synthetic event, called HA-DNA synthesis, upon which subsequent viral RNA and viral hemagglutinin synthesis is dependent, is initiated in late S phase of the infected cell (18). It was postulated that HA-DNA represents parental viral replicative...

journal_title：Journal of virology

authors： Rhode SL 3rd

更新日期：1974-02-01 00:00:00

abstract：:Bluetongue virus (BTV), an insect-vectored emerging pathogen of both wild ruminants and livestock, has had a severe economic impact in agriculture in many parts of the world. The investigation of BTV replication and pathogenesis has been hampered by the lack of a reverse genetics system. Recovery of infectious BTV is ...

journal_title：Journal of virology

authors： Boyce M,Celma CC,Roy P

更新日期：2008-09-01 00:00:00

abstract：:The filoviruses Ebola Zaire virus and Marburg virus are believed to infect target cells through endocytic vesicles, but the details of this pathway are unknown. We used a pseudotyping strategy to investigate the cell biology of filovirus entry. We observed that specific inhibitors of the caveola system, including chol...

journal_title：Journal of virology

authors： Empig CJ,Goldsmith MA

更新日期：2002-05-01 00:00:00

abstract：:Three monoclonal antibodies (MAbs) generated against rainbow trout gonad cells (RTG-2) have been selected for their ability to protect cells from the viral hemorrhagic septicemia virus (VHSV) infection, a salmonid rhabdovirus. Protection from infection was restricted to the salmonid-derived cell lines indicating speci...

journal_title：Journal of virology

authors： Bearzotti M,Delmas B,Lamoureux A,Loustau AM,Chilmonczyk S,Bremont M

更新日期：1999-09-01 00:00:00

abstract：:Chronic infections with viruses such as hepatitis B virus, hepatitis C virus, and HIV constitute a major global public health problem. Studies of chronic viral infections in humans and mice show that persistent antigenic stimulation induces dysregulation of T cell responses; virus-specific T cells either undergo clona...

journal_title：Journal of virology

authors： Sullivan JA,Kim EH,Plisch EH,Suresh M

更新日期：2012-09-01 00:00:00

abstract：:Adenovirus has a linear, double-stranded DNA genome that is perceived by the cellular Mre11-Rad50-Nbs1 (MRN) DNA repair complex as a double-strand break. If unabated, MRN elicits a double-strand break repair response that blocks viral DNA replication and ligates the viral genomes into concatemers. There are two sets o...

journal_title：Journal of virology

authors： Karen KA,Hearing P

更新日期：2011-05-01 00:00:00

abstract：:Envelope glycoprotein E(rns) of classical swine fever virus (CSFV) has been shown to contain RNase activity and is involved in virus infection. Two short regions of amino acids in the sequence of E(rns) are responsible for RNase activity. In both regions, histidine residues appear to be essential for catalysis. They w...

journal_title：Journal of virology

authors： Hulst MM,Panoto FE,Hoekman A,van Gennip HG,Moormann RJ

更新日期：1998-01-01 00:00:00

abstract：:Polyomaviruses are small nonenveloped particles with a circular double-stranded genome, approximately 5 kbp in size. The mammalian polyomaviruses mainly cause persistent subclinical infections in their natural nonimmunocompromised hosts. In contrast, the polyomaviruses of birds--avian polyomavirus (APV) and goose hemo...

journal_title：Journal of virology

authors： Johne R,Wittig W,Fernández-de-Luco D,Höfle U,Müller H

更新日期：2006-04-01 00:00:00

abstract：:Adenovirus type 2 (Ad2) and Ad5 enter epithelial cells via the coxsackievirus B Ad receptor (CAR) and alpha(v) integrin coreceptors. In the absence of CAR, they can be directed to the Fcgamma receptor 1 of hematopoietic cells by an adaptor comprising the extracellular CAR domain and the Fc portion of a human immunoglo...

journal_title：Journal of virology

authors： Meier O,Gastaldelli M,Boucke K,Hemmi S,Greber UF

更新日期：2005-02-01 00:00:00

abstract：:The ATP-dependent DNase activity of Escherichia coli disappeared or was markedly reduced after infection with double-stranded DNA phages, T2, T3, T4, T5, T6, T7, lambda, phi80, and P1, but not with the single-stranded DNA phage f1, or the RNA phage Qbeta. This DNase activity was not reduced when chloramphenicol was ad...

journal_title：Journal of virology

authors： Sakaki Y

更新日期：1974-12-01 00:00:00

abstract：:Although there is increasing evidence that individuals already infected with human immunodeficiency virus type 1 (HIV-1) can be infected with a heterologous strain of the virus, the extent of protection against superinfection conferred by the first infection and the biologic consequences of superinfection are not well...

journal_title：Journal of virology

authors： Yeh WW,Jaru-Ampornpan P,Nevidomskyte D,Asmal M,Rao SS,Buzby AP,Montefiori DC,Korber BT,Letvin NL

更新日期：2009-03-01 00:00:00

abstract：:We tested chemokine receptor subset usage by diverse, well-characterized primary viruses isolated from peripheral blood by monitoring viral replication with CCR1, CCR2b, CCR3, CCR5, and CXCR4 U87MG.CD4 transformed cell lines and STRL33/BONZO/TYMSTR and GPR15/BOB HOS.CD4 transformed cell lines. Primary viruses were iso...

journal_title：Journal of virology

authors： Zhang L,He T,Huang Y,Chen Z,Guo Y,Wu S,Kunstman KJ,Brown RC,Phair JP,Neumann AU,Ho DD,Wolinsky SM

更新日期：1998-11-01 00:00:00

abstract：:Packaging of type C retrovirus genomic RNAs into budding virions requires a highly specific interaction between the viral Gag precursor and unique cis-acting packaging signals on the full-length RNA genome, allowing the selection of this RNA species from among a pool of spliced viral RNAs and similar cellular RNAs. Th...

journal_title：Journal of virology

authors： Harrison GP,Miele G,Hunter E,Lever AM

更新日期：1998-07-01 00:00:00

abstract：UNLABELLED:The increasing prevalence of ranavirus (RV; Iridoviridae) infections of wild and commercially maintained aquatic species is raising considerable concerns. While Xenopus laevis is the leading model for studies of immunity to RV, amphibian antiviral interferon (IFN) responses remain largely uncharacterized. Ac...

journal_title：Journal of virology

authors： Grayfer L,De Jesús Andino F,Robert J

更新日期：2014-05-01 00:00:00

abstract：:To combat emerging coronaviruses, developing safe and efficient platforms to evaluate viral protease activities and the efficacy of protease inhibitors is a high priority. Here, we exploit a biosafety level 2 (BSL-2) chimeric Sindbis virus system to evaluate protease activities and the efficacy of inhibitors directed ...

journal_title：Journal of virology

authors： Deng X,Agnihothram S,Mielech AM,Nichols DB,Wilson MW,StJohn SE,Larsen SD,Mesecar AD,Lenschow DJ,Baric RS,Baker SC

更新日期：2014-10-01 00:00:00

abstract：:We constructed a matched set of plasmids to investigate the interactions of essential core sequences of the simian virus 40 replication origin with flanking regulatory sequences. Deletions of either T-antigen-binding region I or the 21-base-pair repeated promoter elements reduced replication to 50 to 70% of wild-type ...

journal_title：Journal of virology

authors： DeLucia AL,Deb S,Partin K,Tegtmeyer P

更新日期：1986-01-01 00:00:00