Abstract:
:We constructed a matched set of plasmids to investigate the interactions of essential core sequences of the simian virus 40 replication origin with flanking regulatory sequences. Deletions of either T-antigen-binding region I or the 21-base-pair repeated promoter elements reduced replication to 50 to 70% of wild-type levels. The simultaneous deletion of both regions decreased replication to less than 5% of wild-type levels. Thus, the double deletion greatly amplified the defects of the single deletions. We conclude that region I and the 21-base-pair repeats have related rather than independent functions in DNA synthesis. Insertion of a synthetic region I or the adenovirus 2 major late promoter at the late side of isolated core sequences in place of the 21-base-pair repeats failed to restore replication. In contrast, insertion of a single 72-base-pair enhancer element stimulated replication of the core origin more than fivefold. Thus, three distinct regulatory elements appear to facilitate core DNA replication by related mechanisms. Flanking sequences have only a small direct effect on T-antigen binding to naked core DNA. Possible mechanisms of action include the regulation of transcription or of chromatin structure.
journal_name
J Viroljournal_title
Journal of virologyauthors
DeLucia AL,Deb S,Partin K,Tegtmeyer Pdoi
10.1128/JVI.57.1.138-144.1986subject
Has Abstractpub_date
1986-01-01 00:00:00pages
138-44issue
1eissn
0022-538Xissn
1098-5514journal_volume
57pub_type
杂志文章abstract::We previously reported that the cellular transcription factor hypoxia-inducible factor 1α (HIF-1α) binds a hypoxia response element (HRE) located within the promoter of Epstein-Barr virus's (EBV's) latent-lytic switch BZLF1 gene, Zp, inducing viral reactivation. In this study, EBV-infected cell lines derived from gast...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00722-20
更新日期:2020-08-31 00:00:00
abstract::The possible addition of extra sequences to simian virus 40 (SV40) DNA was analyzed by electron microscopy in two different cell systems, productively infected monkey cells and activated heterokaryons on monkey and transformed mouse 3T3 cells. We found that the closed circular DNA fraction, extracted from monkey cells...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.2.323-337.1977
更新日期:1977-08-01 00:00:00
abstract::Information was sought on the temporal distribution of transmissible mink encephalopathy virus in royal pastel mink inoculated subcutaneously with 10(3.0) 50% intracerebral lethal doses of the Idaho strain. As determined by intracerebral assay in mink, extremely little replication of the virus occurred during the prec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3235-3240.1987
更新日期:1987-10-01 00:00:00
abstract::Our knowledge regarding immune-protective and immunopathogenic events in severe acute respiratory syndrome coronavirus (SARS-CoV) infection is limited, and little is known about the dynamics of the immune response at the primary site of disease. Here, an African green monkey (AGM) model was used to elucidate immune me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06791-11
更新日期:2012-04-01 00:00:00
abstract::Protein kinase activity is associated with both the extracellular and the occluded forms of Autographa californica nuclear polyhedrosis virus, a baculovirus. Serine and threonine are the predominant amino acids phosphorylated by the kinase activity associated with both viral forms; no phosphotyrosine was detected. The...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.275-278.1983
更新日期:1983-04-01 00:00:00
abstract::Empty capsids of foot-and-mouth disease virus (FMDV) type A22 Iraq 24/64, whose structure has been solved by X-ray crystallography, are unusual for picornaviruses since they contain VP2 and VP4, the cleavage products of the protein precursor VP0. Both the N terminus of VP1 and the C terminus of VP4, which pack togethe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9743-9752.1997
更新日期:1997-12-01 00:00:00
abstract::Myeloblastosis-associated virus type 1 (MAV1) derived from a molecular clone of infectious proviral DNA (B. Perbal, J. S. Lipsick, J. Svoboda, R. F. Silva, and M. A. Baluda, J. Virol. 56:240-244, 1985) was shown to specifically induce nephroblastoma in chickens and therefore belongs to the MAV-N class. We show that ne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1803-1807.1989
更新日期:1989-04-01 00:00:00
abstract::As viruses are reliant upon their host cell to serve as proper environments for their replication, many have evolved mechanisms to alter intracellular conditions to suit their own needs. For example, human cytomegalovirus induces quiescent cells to enter the cell cycle and then arrests them in late G(1), before they e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.6.3451-3459.2003
更新日期:2003-03-01 00:00:00
abstract::The cellular protein bone marrow stromal antigen-2 (BST-2)/tetherin acts against a variety of enveloped viruses by restricting their release from the plasma membrane. The HIV-1 accessory protein Vpu counteracts BST-2 by downregulating it from the cell surface and displacing it from virion assembly sites. Previous comp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02315-18
更新日期:2019-05-15 00:00:00
abstract::Influenza virus has a set of ribonucleoproteins (RNPs) consisting of viral RNAs, influenza virus polymerase subunits, and nucleoprotein. Intracellular reconstitution of the whole set of RNPs via plasmid transfection results in the generation of influenza virus. By the use of reverse genetics and dual promoters, we con...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00781-09
更新日期:2009-09-01 00:00:00
abstract::In herpes simplex virus type 2, the mRNAs of ICP4 and ICP22/47 are divergently transcribed and their transcription initiation sites are separated by 750 base pairs (L. J. Whitton and J. B. Clements, Nucleic Acids Res. 12:2061-2078, 1984). This 750-base-pair region contains many recognized cis-acting elements, includin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2036-2047.1989
更新日期:1989-05-01 00:00:00
abstract::Nuclei of cells infected with Moloney murine leukemia virus (MoMuLV) were examined for the presence of gag proteins. This analysis was performed in conjunction with other studies suggesting a possible role for gag proteins in regulating nuclear events relating to processing and/or transport of viral genomic RNA. We de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1350-1356.1993
更新日期:1993-03-01 00:00:00
abstract::At a low pH, the influenza virus hemagglutinin (HA) undergoes conformational changes that promote membrane fusion. While the critical role of fusion peptide release from the trimer interface has been demonstrated previously, the role of globular head dissociation in the overall fusion mechanism remains unclear. To inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.4940-4950.1992
更新日期:1992-08-01 00:00:00
abstract::Herpesviruses encode a set of core proteins essential for lytic replication of their genomes. Three of these proteins form a tripartite helix-primase complex that, in the case of Epstein-Barr virus (EBV), consists of the helicase BBLF4, the primase BSLF1, and the linker protein BBLF2/3. BBLF2/3 and its homologs in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.245-256.2005
更新日期:2005-01-01 00:00:00
abstract::The virion proteins encoded by the avian retroviral pol gene (reverse transcriptase and endonuclease) are formed by the proteolytic processing of a gag-pol fusion protein precursor. Recent studies have predicted that the avian sarcoma-leukosis virus pol precursor protein undergoes a previously undetected processing ev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.528-533.1988
更新日期:1988-02-01 00:00:00
abstract::Disulfide bonds reportedly stabilize the capsids of several viruses, including papillomavirus, polyomavirus, and simian virus 40, and have been detected in herpes simplex virus (HSV) capsids. In this study, we show that in mature HSV-1 virions, capsid proteins VP5, VP23, VP19C, UL17, and UL25 participate in covalent c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00214-11
更新日期:2011-09-01 00:00:00
abstract::Intracisternal-A-particle-related envelope-encoding (IAPE) proviral elements in the mouse genome encode and express an envelope-like protein that may allow transmission of IAPEs as infectious agents. To test IAPE mobility and potential transmission in mice, we have analyzed the distribution of IAPE elements in the gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6450-6454.1996
更新日期:1996-09-01 00:00:00
abstract::RNA-dependent RNA polymerase activity was found in mouse hepatitis virus strain A59 (MHV-A59)-infected cells. The enzyme was induced in the infected cells and could not be detected in the MHV-A59 virion. Two peaks of RNA polymerase activity, one early and the other late in infection, were detected. These polymerase ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.3.847-853.1982
更新日期:1982-06-01 00:00:00
abstract::The Ad2+ND4 virus is an adenovirus type 2 (Ad2)-simian virus 40 (SV40) recombination. The Ad2 genome of this recombinant has a rearrangement within early region 3; Ad2 DNA sequences between map positions 81.3 and 85.5 have been deleted, and the SV40 DNA sequences between map positions 0.11 and 0.626 have been inserted...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.2.344-353.1983
更新日期:1983-08-01 00:00:00
abstract::The activity of human immunodeficiency virus Rev as a regulator of viral mRNA expression is tightly linked to its ability to shuttle between the nucleus and cytoplasm; these properties are conferred by a leucine-rich nuclear export signal (NES) and by an arginine-rich nuclear localization signal/RNA binding domain (NL...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11899-11910.2000
更新日期:2000-12-01 00:00:00
abstract::The molecular mechanisms responsible for random integration and gene targeting by recombinant adeno-associated virus (AAV) vectors are largely unknown, and whether vectors derived from autonomous parvoviruses transduce cells by similar pathways has not been investigated. In this report, we constructed vectors based on...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.13136-13145.2003
更新日期:2003-12-01 00:00:00
abstract::Immunosuppressed patients are at risk for developing Epstein-Barr Virus (EBV)-positive lymphomas that express the major EBV oncoprotein, LMP1. Although increasing evidence suggests that a small number of lytically infected cells may promote EBV-positive lymphomas, the impact of enhanced lytic gene expression on the ab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00770-12
更新日期:2012-08-01 00:00:00
abstract::The herpes simplex virus 1 (HSV-1) multifunctional regulatory protein ICP27 shuttles between the nucleus and cytoplasm in its role as a viral mRNA export factor. Arginine methylation on glycine- and arginine-rich motifs has been shown to regulate protein export. ICP27 contains an RGG box and has been shown to be methy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00238-09
更新日期:2009-06-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is the most important cause of severe, lower respiratory tract infections in infants, and RSV infections have been associated with chronic wheezing and asthma during childhood. However, the mechanism of RSV-induced airway inflammation and airway hyperresponsiveness (AHR) is poorly und...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05869-11
更新日期:2011-12-01 00:00:00
abstract::Assembly and maturation of retroviral particles requires the aggregation and controlled proteolytic cleavage of polyprotein core precursors by a precursor-encoded protease (PR). Active, mature retroviral PR is a dimer, and the accumulation of precursors at sites of assembly may facilitate subunit interaction and subse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6165-6172.1991
更新日期:1991-11-01 00:00:00
abstract::Recent studies have demonstrated that influenza A virus infection activates the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway by binding of influenza NS1 protein to the p85 regulatory subunit of PI3K. Our previous study proposed that two polyproline motifs in NS1 (amino acids 164 to 167 [PXXP], SH3 bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01427-07
更新日期:2007-12-01 00:00:00
abstract::We have examined the relative ability of Sendai virus M (matrix) protein to associate with membranes containing viral glycoproteins at three distinct stages of the exocytic pathway prior to cell surface appearance. By the use of selective low-temperature incubations or the ionophore monensin, the transport of newly sy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.651-663.1993
更新日期:1993-02-01 00:00:00
abstract::In the course of our studies on oxidative stress as a component of pathological processes in humans, we showed that microintrusion into cells with microcapillary and ultramicroelectrochemical detection could mimic many types of mechanical intrusion leading to an instant (0.1 s) and high (some femtomoles) burst release...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1447-1452.1999
更新日期:1999-02-01 00:00:00
abstract::Viral protein genome-linked (VPg) plays a central role in several stages of potyvirus infection. This study sought to answer questions about the role of Potato virus A (PVA; genus Potyvirus) VPg in viral and host RNA expression. When expressed in Nicotiana benthamiana leaves in trans, a dual role of VPg in translation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00052-11
更新日期:2011-09-01 00:00:00
abstract:UNLABELLED:We have recently shown in both herpesviruses and phages that packaged viral DNA creates a pressure of tens of atmospheres pushing against the interior capsid wall. For the first time, using differential scanning microcalorimetry, we directly measured the energy powering the release of pressurized DNA from th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01172-15
更新日期:2015-09-01 00:00:00