Abstract:
:Viral protein genome-linked (VPg) plays a central role in several stages of potyvirus infection. This study sought to answer questions about the role of Potato virus A (PVA; genus Potyvirus) VPg in viral and host RNA expression. When expressed in Nicotiana benthamiana leaves in trans, a dual role of VPg in translation is observed. It repressed the expression of monocistronic luciferase (luc) mRNA and simultaneously induced a significant upregulation in the expression of both replicating and nonreplicating PVA RNAs. This enhanced viral gene expression was due at least to the 5' untranslated region (UTR) of PVA RNA, eukaryotic initiation factors 4E and iso 4E [eIF4E/eIF(iso)4E], and the presence of a sufficient amount of VPg. Coexpression of VPg with viral RNA increased the viral RNA amount, which was not the case with the monocistronic mRNA. Both mutations at certain lysine residues in PVA VPg and eIF4E/eIF(iso)4E depletion reduced its ability to upregulate the viral RNA expression. These modifications were also involved in VPg-mediated downregulation of monocistronic luc expression. These results suggest that VPg can titrate eIF4Es from capped monocistronic RNAs. Because VPg-mediated enhancement of viral gene expression required eIF4Es, it is possible that VPg directs eIF4Es to promote viral RNA expression. From this study it is evident that VPg can serve as a specific regulator of PVA expression by boosting the viral RNA amounts as well as the accumulation of viral translation products. Such a mechanism could function to protect viral RNA from being degraded and to secure efficient production of coat protein (CP) for virion formation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Eskelin K,Hafrén A,Rantalainen KI,Mäkinen Kdoi
10.1128/JVI.00052-11subject
Has Abstractpub_date
2011-09-01 00:00:00pages
9210-21issue
17eissn
0022-538Xissn
1098-5514pii
JVI.00052-11journal_volume
85pub_type
杂志文章abstract::By marker rescue of bacteriophage SP02 sus mutants with purified bacteriophage SP02 DNA fragments, 11 of the 17 known bacteriophage SP02 sus loci were assigned to discrete DNA fragments. The left-most genetic locus, susA, was found to reside near one bacteriophage SP02 terminus (EcoRI-C1 fragment), whereas the right-m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.131-134.1982
更新日期:1982-04-01 00:00:00
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更新日期:1998-01-01 00:00:00
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更新日期:1980-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4745-4751.1988
更新日期:1988-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.6.1608-1614.1975
更新日期:1975-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2018-07-31 00:00:00
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pub_type: 杂志文章
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更新日期:2019-06-14 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01496-09
更新日期:2010-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.866-876.1980
更新日期:1980-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
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doi:10.1128/JVI.73.11.9137-9144.1999
更新日期:1999-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.6251-6256.1998
更新日期:1998-07-01 00:00:00
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journal_title:Journal of virology
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更新日期:2014-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,随机对照试验
doi:10.1128/JVI.00073-11
更新日期:2011-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2083-2090.1993
更新日期:1993-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.664-671.1972
更新日期:1972-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.303-309.1984
更新日期:1984-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2006-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2006-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01971-14
更新日期:2014-11-01 00:00:00
abstract::To determine the extent and structure of genetic variation in dengue viruses (DENV) on a restricted spatial and temporal scale, we sequenced the E (envelope) genes of DENV-1, -2, and -3 isolates collected in 2001 from children enrolled in a prospective school-based study in Kamphaeng Phet, Thailand, and diagnosed with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02728-07
更新日期:2008-06-01 00:00:00
abstract::The objective of this investigation was to examine by electron microscopy the replicative ribonucleoprotein (RNP) structures synthesized in vesicular stomatitis virus-infected HeLa cells. Pulse-labeled in vivo products of vesicular stomatitis virus replication and transcription can be separated by centrifugation in Re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.3.764-771.1980
更新日期:1980-06-01 00:00:00