Abstract:
:By marker rescue of bacteriophage SP02 sus mutants with purified bacteriophage SP02 DNA fragments, 11 of the 17 known bacteriophage SP02 sus loci were assigned to discrete DNA fragments. The left-most genetic locus, susA, was found to reside near one bacteriophage SP02 terminus (EcoRI-C1 fragment), whereas the right-most genetic locus, susP, was found to reside near the other bacteriophage SP02 terminus (EcoRI-C2 fragment). The physical locations of the intervening genetic loci were found to be consistent with the previously determined genetic order. Evidence was also obtained which suggested that at least one end of a transforming DNA fragment is degraded during DNA uptake by the competent bacterium.
journal_name
J Viroljournal_title
Journal of virologyauthors
Graham S,Sutton S,Yoneda Y,Young FEdoi
10.1128/JVI.42.1.131-134.1982subject
Has Abstractpub_date
1982-04-01 00:00:00pages
131-4issue
1eissn
0022-538Xissn
1098-5514journal_volume
42pub_type
杂志文章abstract::The binding of a recombinant soluble form of the measles virus (MV) hemagglutinin (sH) to cells expressing hybrid CD46/CD4 proteins was compared to that of purified virus. For binding of both ligands, both CD46 external short consensus repeats I and II (SCR I and II) in the natural order were essential. The addition o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.4157-4160.1997
更新日期:1997-05-01 00:00:00
abstract::Among several proposed cellular receptors for bovine viral diarrhea virus (BVDV), the low-density lipoprotein (LDL) receptor is of special interest because it is also considered a receptor for the related hepatitis C virus. It has been reported that an anti-LDL receptor monoclonal antibody blocked the infection of bov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01589-06
更新日期:2006-11-01 00:00:00
abstract::The cytomegalovirus (CMV) serine proteinase assemblin is synthesized as a precursor that undergoes three principal autoproteolytic cleavages. Two of these are common to the assemblin homologs of all herpes group viruses: one at the maturational site near the carboxyl end of the precursor and another at the release sit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5395-5404.1996
更新日期:1996-08-01 00:00:00
abstract::Due to the limited ultrastructural information about the coronavirion, little is known about the interactions acting at the interface between nucleocapsid and viral envelope. Knowing that subtle mutations in the carboxy-terminal endodomain of the M protein are already lethal, we have now probed the equivalent domain o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7369-7378.2004
更新日期:2004-07-01 00:00:00
abstract::The cellular tropism of the feline immunodeficiency virus (FIV) is affected by changes in variable region 3 (V3) of the surface (SU) envelope glycoprotein (Verschoor, E. J., et al., J. Virol. 69:4752-4757, 1995). By using high-dose DNA transfection, an FIV molecular clone with a non-CRFK-tropic V3 acquired the ability...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.7132-7135.1997
更新日期:1997-09-01 00:00:00
abstract::The proteolytic enzyme, thermolysin, degraded the external segment of the membrane glycoprotein of intact vesicular stomatitis (VS) virions but left behind a small nonglycosylated fragment, presumably embedded in the virion membrane. Other proteases generated membrane-associated glycoprotein fragments differing somewh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.2.237-240.1975
更新日期:1975-08-01 00:00:00
abstract:UNLABELLED:A bat virus with high phylogenetic relatedness to human mumps virus (MuV) was identified recently at the nucleic acid level. We analyzed the functional activities of the hemagglutinin-neuraminidase (HN) and the fusion (F) proteins of the bat virus (batMuV) and compared them to the respective proteins of a hu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03693-14
更新日期:2015-04-01 00:00:00
abstract::The enzymatic activity of recombinant influenza virus RNA polymerase is strictly dependent on the addition of a template RNA containing 5' and 3' viral sequences. Here we report the analysis of the binding specificity and physical characterization of the complex by using gel shift, modification interference, and densi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5108-5116.1994
更新日期:1994-08-01 00:00:00
abstract::The restrictive properties of tripartite motif-containing 5 alpha (TRIM5α) from small ruminant species have not been explored. Here, we identify highly similar TRIM5α sequences in sheep and goats. Cells transduced with ovine TRIM5α effectively restricted the lentivirus visna/maedi virus DNA synthesis. Proteasome inhib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00440-12
更新日期:2012-09-01 00:00:00
abstract::To identify the domains of Vpr that are involved nuclear localization, we transfected HeLa cells with a panel of expression vectors that encode mutant Vpr protein with deletions or substitutions within putative domains. Immunofluorescence staining of transfected cells revealed that wild-type Vpr was localized predomin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.7179-7186.2000
更新日期:2000-08-01 00:00:00
abstract::Varicella-zoster virus DNA was digested with 11 restriction endonucleases, and the resulting fragments were separated on agarose gels. Terminal fragments were identified by lambda exonuclease digestion. Physical maps were then constructed using a combination of double restriction enzyme digestion and hybridization to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.2.615-618.1984
更新日期:1984-05-01 00:00:00
abstract::We followed the dynamics of capsid amino acid replacement among 403 Nigerian outbreak isolates of type 2 circulating vaccine-derived poliovirus (cVDPV2) from 2005 through 2011. Four different functional domains were analyzed: (i) neutralizing antigenic (NAg) sites, (ii) residues binding the poliovirus receptor (PVR), ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01949-17
更新日期:2018-04-13 00:00:00
abstract::We provide here new insights into rotavirus (RRV) pathogenicity by showing that RRV infection promotes structural and functional injuries localized at the tight junctions (TJ) in the cell-cell junctional complex of cultured polarized human intestinal Caco-2 cells forming monolayers. RRV infection resulted in a progres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4645-4651.2000
更新日期:2000-05-01 00:00:00
abstract::The integrin alpha(v)beta3 has been shown to act as the receptor for internalization of foot-and-mouth disease virus (FMDV) (A12), with attachment being through a highly conserved RGD motif located on the G-H loop of viral capsid protein VP1. In addition, however, we have recently shown that efficient infection of cul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8357-8361.1997
更新日期:1997-11-01 00:00:00
abstract::We report here that sex hormones modulate susceptibility to a sexually transmitted viral agent, herpes simplex virus type 2 (HSV-2), in a mouse model. Ovariectomized mice were administered either saline (control), estradiol (E(2)), progesterone (P(4)), or a combination of both estradiol and progesterone (E+P) and infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.3107-3116.2005
更新日期:2005-03-01 00:00:00
abstract:UNLABELLED:Recent experiments suggest that some glycoprotein (GP)-specific monoclonal antibodies (MAbs) can protect experimental animals against the filovirus Ebola virus (EBOV). There is a need for isolation of MAbs capable of neutralizing multiple filoviruses. Antibody neutralization assays for filoviruses frequently...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00101-16
更新日期:2016-03-28 00:00:00
abstract::We molecularly cloned unintegrated viral DNA of the BALB/c endogenous N-tropic and B-tropic murine leukemia retroviruses and in vitro passaged N-tropic Gross (passage A) murine leukemia retroviruses. Recombinant genomes were constructed in vitro by exchanging homologous restriction enzyme fragments from N- or B-tropic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.110-119.1983
更新日期:1983-10-01 00:00:00
abstract::After isolation, the DNA of simian virus 40 appeared as a negative supertwist (form I) or as an open circle with at least one single-strand scission (form II). Under the denaturation conditions usually applied, such as heating in the presence of formaldehyde or application of alkali, form I molecules could appear as "...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.585-598.1975
更新日期:1975-03-01 00:00:00
abstract::Viral myocarditis is an important human disease, and reovirus-induced murine myocarditis provides an excellent model system for study. Cardiac myocytes, like neurons in the central nervous system, are not replenished, yet there is no cardiac protective equivalent to the blood-brain barrier. Thus, cardiac myocytes may ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01172-07
更新日期:2007-12-01 00:00:00
abstract::The structural polypeptides of purified Plodia interpunctella granulosis virus were analyzed by three different two-dimensional gel systems. Isoelectric focusing followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis allowed resolution of 53 acidic polypeptides in the enveloped nucleocapsid of the virus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.1.82-89.1986
更新日期:1986-10-01 00:00:00
abstract:UNLABELLED:PML is the organizer of cellular structures termed nuclear domain 10 (ND10) or PML-nuclear bodies (PML-NBs) that act as key mediators of intrinsic immunity against human cytomegalovirus (HCMV) and other viruses. The antiviral function of ND10 is antagonized by viral regulatory proteins such as the immediate ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01973-15
更新日期:2015-11-11 00:00:00
abstract::The role of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein IE0 in the baculoviral infection is not clear. In this study, we constructed the recombinant virus vAcDeltaie0 null for ie0 expression by targeted mutagenesis replacing exon0 with the cat gene. We found that vAcDeltai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.10077-10082.2005
更新日期:2005-08-01 00:00:00
abstract::West Nile virus (WNV) is a human pathogen that can cause neurological disorders, including meningoencephalitis. Experiments with mice and mammalian cell cultures revealed that WNV exhibited resistance to the innate immune program induced by alpha interferon (IFN-alpha). We have investigated the nature of this inhibiti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1343-1350.2005
更新日期:2005-02-01 00:00:00
abstract::Herpes simplex virus type 2 (HSV-2) glycoprotein B (gB-2) gene segments were expressed as recombinant proteins in Escherichia coli. gB-2 recombinant proteins were reacted with human serum immunoglobulin G (IgG) antibodies in Western immunoblot assays. Initially, samples were tested for the presence of HSV-1-specific a...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.70.5.2950-2956.1996
更新日期:1996-05-01 00:00:00
abstract::Nuclear domains called ND10 (nuclear domain 10) are discrete nuclear protein aggregations characterized by a set of interferon-upregulated proteins including Sp100 and PML, where papova-, adeno-, and herpesviruses begin their transcription and DNA replication. Both the alpha- and betaherpesvirus subfamilies disrupt ND...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11800-11810.2000
更新日期:2000-12-01 00:00:00
abstract::RNA plant viruses use various translational regulatory mechanisms to control their gene expression. Translational enhancement of viral mRNAs that leads to higher levels of protein synthesis from specific genes may be essential for the virus to successfully compete for cellular translational machinery. The control elem...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1144-1153.2002
更新日期:2002-02-01 00:00:00
abstract:UNLABELLED:Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and characterized by a high rate of abortion in ruminants and hemorrhagic fever, encephalitis, or blindness in humans. RVF is caused by Rift Valley fever virus (RVFV; family Bunyaviridae, genus Phlebovirus), which has a tripartite...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00135-15
更新日期:2015-07-01 00:00:00
abstract::The unit-length genome of human herpesvirus 6 (HHV-6) consists of a single unique component (U) bounded by direct repeats DRL and DRR and forms head-to-tail concatemers during productive infection. cis-elements which mediate cleavage and packaging of progeny virions (a sequences) are found at the termini of all herpes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3007-3014.1994
更新日期:1994-05-01 00:00:00
abstract::BK polyomavirus (BKV) establishes persistent, low-level, and asymptomatic infections in most humans and causes polyomavirus-associated nephropathy (PVAN) and other pathologies in some individuals. The activation of BKV replication following kidney transplantation, leading to viruria, viremia, and, ultimately, PVAN, is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06369-11
更新日期:2012-03-01 00:00:00
abstract::The DA strain and other members of the TO subgroup of Theiler's murine encephalomyelitis virus (TMEV) induce an early transient subclinical neuronal disease followed by a chronic progressive inflammatory demyelination, with persistence of the virus in the central nervous system (CNS) for the life of the mouse. Althoug...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00178-11
更新日期:2011-09-01 00:00:00