Abstract:
:RNA plant viruses use various translational regulatory mechanisms to control their gene expression. Translational enhancement of viral mRNAs that leads to higher levels of protein synthesis from specific genes may be essential for the virus to successfully compete for cellular translational machinery. The control elements have yet to be analyzed for members of the genus Carmovirus, a small group of plant viruses with positive-sense RNA genomes. In this study, we examined the 3' untranslated region (UTR) of hibiscus chlorotic ringspot virus (HCRSV) genomic RNA (gRNA) and subgenomic RNA (sgRNA) for its role in the translational regulation of viral gene expression. The results showed that the 3' UTR of HCRSV significantly enhanced the translation of several open reading frames on gRNA and sgRNA and a viral gene in a bicistronic construct with an inserted internal ribosome entry site. Through deletion and mutagenesis studies of both the bicistronic construct and full-length gRNA, we demonstrated that a six-nucleotide sequence, GGGCAG, that is complementary to the 3' region of the 18S rRNA and a minimal length of 180 nucleotides are required for the enhancement of translation induced by the 3' UTR.
journal_name
J Viroljournal_title
Journal of virologyauthors
Koh DC,Liu DX,Wong SMdoi
10.1128/jvi.76.3.1144-1153.2002subject
Has Abstractpub_date
2002-02-01 00:00:00pages
1144-53issue
3eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
杂志文章abstract::Transcription of the terminal protein (TP) gene of Epstein-Barr virus (EBV) in Burkitt's lymphoma cells, in EBV-negative Burkitt's lymphoma cells converted with transformation-defective (P3HR1) and transformation-competent (B95-8, AG876) EBV strains, and in EBV-immortalized cell lines was studied. A TP1 cDNA probe spa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.415-423.1991
更新日期:1991-01-01 00:00:00
abstract::Novel molecular approaches to inhibit human immunodeficiency virus type 1 (HIV-1) infection have received increasing attention because of the lack of effective antiviral drug therapies in vivo. We now demonstrate that cells can be intracellularly immunized by cytoplasmic expression of single-chain variable antibody fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3392-3400.1996
更新日期:1996-06-01 00:00:00
abstract::Here we present the first complete genomic sequence of Marek's disease virus serotype 3 (MDV3), also known as turkey herpesvirus (HVT). The 159,160-bp genome encodes an estimated 99 putative proteins and resembles alphaherpesviruses in genomic organization and gene content. HVT is very similar to MDV1 and MDV2 within ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.971-978.2001
更新日期:2001-01-01 00:00:00
abstract::The second of three short upstream open reading frames (uORF2) in the transcript leader of the human cytomegalovirus gp48 (gpUL4) virion glycoprotein gene inhibits downstream translation approximately 10-fold. Remarkably, this inhibition depends on the amino acid coding information of uORF2. In the current studies we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1030-1036.1995
更新日期:1995-02-01 00:00:00
abstract::The ORF3 protein of hepatitis E virus (HEV) is a multifunctional protein important for virus replication. The ORF3 proteins from human, swine, and avian strains of HEV contain a conserved PXXP amino acid motif, resembling either Src homology 3 (SH3) cell signaling interaction motifs or "late domains" involved in host ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06711-11
更新日期:2012-05-01 00:00:00
abstract::A soluble polymerase-template complex prepared from poliovirus-infected cells was found to incorporate radioactive UTP into trichloroacetic acid-insoluble RNA linearly for 8 h in the presence of ATP and Mg2+. Radioactive CTP or GTP was not incorporated under identical conditions. Nearest-neighbor analysis of the in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.61-68.1977
更新日期:1977-01-01 00:00:00
abstract::The C-terminal half of the replicase ORF1a polyprotein of the arterivirus equine arteritis virus is processed by a chymotrypsinlike serine protease (SP) (E. J. Snijder et al., J. Biol. Chem. 271:4864-4871, 1996) located in nonstructural protein 4 (nsp4). Three probable SP cleavage sites had previously been identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9313-9322.1997
更新日期:1997-12-01 00:00:00
abstract::The gD, gB, and gH/gL glycoprotein quartet constitutes the basic apparatus for herpes simplex virus (HSV) entry into the cell and fusion. gD serves as a receptor binding glycoprotein and trigger of fusion. The conserved gB and gH/gL execute fusion. Central to understanding HSV entry/fusion has become the dissection of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01287-09
更新日期:2009-10-01 00:00:00
abstract::Using a recently developed model for in vitro generation of pp65-positive polymorphonuclear leukocytes (PMNLs), we demonstrated that PMNLs from immunocompetent subjects may harbor both infectious human cytomegalovirus (HCMV) and viral products (pp65, p72, DNA, and immediate-early [IE] and pp67 late mRNAs) as early as ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5629-5638.2000
更新日期:2000-06-01 00:00:00
abstract::Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is known to take an endosomal pathway for cell entry; however, it is thought to enter directly from the cell surface when a receptor-bound virion spike (S) protein is affected by trypsin, which induces cleavage of the S protein and activates its fusion po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01412-08
更新日期:2008-12-01 00:00:00
abstract::A uniform nomenclature has been agreed upon for monoclonal antibodies directed against virus-coded proteins of simian virus 40 and polyoma virus. Blocks of numbers from PAb1 to PAb999 have been allocated to workers involved in the isolation of monoclonal antibodies of this type. The correspondence between PAb numbers ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.709-709.1982
更新日期:1982-02-01 00:00:00
abstract::The evolution of human immunodeficiency virus (HIV) type 1 nef quasispecies in a patient clonally infected with a contaminated batch of blood clotting factor IX was monitored. nef sequences were derived at 11, 25, and 41 months postinfection from infected peripheral blood mononuclear cells after molecular cloning of P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4233-4240.1997
更新日期:1997-06-01 00:00:00
abstract::Examination of the interaction between human immunodeficiency virus (HIV) regulatory gene products and the host immune system is fundamental to understanding the pathogenesis of HIV and could reveal possible targets for therapeutic intervention in the treatment of AIDS. The HIV Tat gene is a potential candidate for th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7622-7629.1995
更新日期:1995-12-01 00:00:00
abstract::A retrovirus vector has been developed that selects for instances in which the provirus integrates in close proximity to cellular promoters. Coding sequences for a selectable marker (histidinol dehydrogenase) were inserted into the 3' long terminal repeat (LTR) of an enhancerless Molony murine leukemia virus. Although...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3227-3233.1989
更新日期:1989-08-01 00:00:00
abstract::Human papillomavirus type 18 (HPV18) and HPV45 account for approximately 20% of all cervix cancers. We show that HPV18, HPV45, and the recently discovered HPV97 comprise a clade sharing a most recent common ancestor within HPV alpha7 species. Variant lineages of these HPV types were classified by sequence analysis of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02068-08
更新日期:2009-02-01 00:00:00
abstract::Mutants of bacteriophage T4D that are defective in genes 42 (dCMP hydroxymethylase), 46 (DNA exonuclease), and 56 (dCTPase) produce limited amounts of phage DNA in Escherichia coli B. In this DNA, glucoylated 5-hydroxymethylcytosine is completely replaced by cytosine. We found that this DNA rapidly becomes fragmented ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.18-30.1983
更新日期:1983-10-01 00:00:00
abstract::Neurons of the sensory ganglia are the major site of varicella-zoster virus (VZV) latency and may undergo productive infection during reactivation. Although the VZV glycoprotein E/glycoprotein I (gE/gI) complex is known to be critical for neurovirulence, few studies have assessed the roles of these proteins during inf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02293-13
更新日期:2013-12-01 00:00:00
abstract::The TAATGARAT motif in the herpes simplex virus (HSV) immediate-early (IE) gene promoters plays a key role in their activation by the Oct-1-Vmw65 complex, but its role in mediating inhibitory effects of cellular octamer-binding proteins is less clear. We have used indicator viruses containing reporter constructs with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3495-3500.1998
更新日期:1998-04-01 00:00:00
abstract::The human T-cell leukemia virus (HTLV) Rex protein is essential for efficient expression of the viral structural and enzymatic gene products. In this study, we assessed the role of the HTLV-2 rex gene in viral RNA expression and Gag protein production. Following transfection of human JM4 T cells with wild-type and rex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8112-8119.1999
更新日期:1999-10-01 00:00:00
abstract::Coronavirus envelope (E) proteins are small (approximately 75- to 110-amino-acid) membrane proteins that have a short hydrophilic amino terminus, a relatively long hydrophobic membrane domain, and a long hydrophilic carboxy-terminal domain. The protein is a minor virion structural component that plays an important, no...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01472-06
更新日期:2007-04-01 00:00:00
abstract::Mumps virus, like other paramyxoviruses in the Rubulavirus genus, encodes a V protein that can assemble a ubiquitin ligase complex from cellular components, leading to the destruction of cellular signal transducer and activator of transcription (STAT) proteins. While many V proteins target the interferon-activated STA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00596-09
更新日期:2009-07-01 00:00:00
abstract::HTG2 hamster cells produce a defective murine sarcoma virus lacking gp70 and, consequently, viral surface projections (knobs), but the lack of knobs appears to have no effect on intramembrane particle distribution. In addition, it has been noted that the core of the virus remains in the "immature" form as a result of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.3.872-877.1980
更新日期:1980-12-01 00:00:00
abstract::Besides its role in viral assembly, the vesicular stomatitis virus (VSV) matrix (M) protein causes cytopathic effects such as cell rounding (D. Blondel, G. G. Harmison, and M. Schubert, J. Virol. 64:1716-1725, 1990). DNA cotransfection assays demonstrated that VSV M protein was able to inhibit the transcription of a r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3529-3537.1995
更新日期:1995-06-01 00:00:00
abstract::The influenza A virus (IAV) envelope protein hemagglutinin binds α2,6- or α2,3-linked sialic acid as a host cell receptor. Bat IAV subtypes H17N10 and H18N11 form an exception to this rule and do not bind sialic acid but enter cells via major histocompatibility complex (MHC) class II. Here, we review current knowledge...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01357-19
更新日期:2020-01-31 00:00:00
abstract::The basis for the switch from CCR5 to CXCR4 coreceptor usage seen in approximately 50% of human immunodeficiency virus type 1 (HIV-1) subtype B-infected individuals as disease advances is not well understood. Among the reasons proposed are target cell limitation and better immune recognition of the CXCR4 (X4)-tropic c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00759-07
更新日期:2007-08-01 00:00:00
abstract::Although picornavirus RNA genomes contain a 3'-terminal poly(A) tract that is critical for their replication, the impact of encephalomyocarditis virus (EMCV) infection on the host poly(A)-binding protein (PABP) remains unknown. Here, we establish that EMCV infection stimulates site-specific PABP proteolysis, resulting...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00896-12
更新日期:2012-10-01 00:00:00
abstract::Lipid biosynthesis was measured in cultured chicken embryo cells after infection with fowlpox virus. Between 24 and 72 h postinfection, fowlpox virus-infected cells incorporated less [14C]acetate and 3H2O into fatty acids and sterols than did mock-infected cells, demonstrating a virus-dependent inhibition of general l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.2.749-752.1982
更新日期:1982-08-01 00:00:00
abstract::A particles with the diameter of 70 to 80 nm were isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type. Most of the A particles banded at 1.23 to 1.24 g/ml, whereas 3 to 10% banded at 1.29 g/ml in equilibrium sucrose gradients. They banded at 1.30 g/ml in CsCl gradi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.4.924-933.1974
更新日期:1974-10-01 00:00:00
abstract::Long-term ovine fibroblast cultures contain replicative-competent lentiviruses in a latent form. This in vitro phenomenon, never described previously for lentiviruses, was clearly demonstrated by activating the expression of latent viruses with various inducing cell treatments, some of which were efficient in inducing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.680-682.1984
更新日期:1984-11-01 00:00:00
abstract::The adenovirus major late promoter (MLP) is active during both the early and late phases of infection. During the early phase the activity of the MLP is similar to those of the other early viral promoters, but during the late phase the rate of transcription from the MLP becomes much greater by comparison. We report he...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.51-60.1990
更新日期:1990-01-01 00:00:00