Abstract:
:The TAATGARAT motif in the herpes simplex virus (HSV) immediate-early (IE) gene promoters plays a key role in their activation by the Oct-1-Vmw65 complex, but its role in mediating inhibitory effects of cellular octamer-binding proteins is less clear. We have used indicator viruses containing reporter constructs with different IE promoters driving a reporter beta-galactosidase gene within the viral genome to investigate this. We showed that deletion of the upstream IE promoter region containing the TAATGARAT motifs abolishes the inhibitory effect of the cellular octamer-binding proteins Oct-2.4 and Oct-2.5 on the viral IE promoter. This inhibitory effect can be restored by addition of a single TAATGARAT motif to the minimal promoter within the viral genome. Hence, the TAATGARAT motif can indeed mediate both positive and negative effects of cellular transcription factors when it is located within the viral genome.
journal_name
J Viroljournal_title
Journal of virologyauthors
Thomas S,Coffin RS,Watts P,Gough G,Latchman DSdoi
10.1128/JVI.72.4.3495-3500.1998subject
Has Abstractpub_date
1998-04-01 00:00:00pages
3495-500issue
4eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
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