Abstract:
:Herpesviruses are capable of several types of infection of a host cell. To investigate the early events which ultimately determine the nature of the virus-host cell interaction, a system was established utilizing temperature-sensitive mutants of herpes simplex virus type 2. Four mutants have been isolated which fail to induce cytopathic effects and do not replicate at 39 C in hamster embryo fibroblast cells. At least one mutant is virus DNA negative. Since intracellular complementation is detectable between pairs of mutants, a virus function is known to be temperature sensitive. However, all four mutants induce cytopathic effects and replicate to parental virus levels in rabbit kidney cells at 39 C. This suggests that a host cell function, lacking or nonfunctional in HEF cells but present in rabbit kidney cells at 39 C, is required for the replication of these mutants in hamster embryo fibroblasts cells at 39 C. Therefore, we conclude that these mutants are both temperature sensitive and exhibit host range properties.
journal_name
J Viroljournal_title
Journal of virologyauthors
Koment RW,Rapp Fdoi
10.1128/JVI.15.4.812-819.1975subject
Has Abstractpub_date
1975-04-01 00:00:00pages
812-9issue
4eissn
0022-538Xissn
1098-5514journal_volume
15pub_type
杂志文章abstract:UNLABELLED:During hepatitis C virus (HCV) infection, broadly neutralizing antibody (bNAb) responses targeting E1E2 envelope glycoproteins are generated in many individuals. It is unclear if these antibodies play a protective or a pathogenic role during chronic infection. In this study, we investigated whether bNAb resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02669-15
更新日期:2016-04-14 00:00:00
abstract::Among Moloney murine leukemia viruses (Mo-MuLVs) having stop codons other than UAG at the gag-pol junction, Mo-MuLV with UAA, but not with UGA, had a replication disadvantage. Mo-MuLV with a glutamine codon (CAG) at the junction did not replicate. A revertant of this virus consisted of the original virus and a virus w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6376-6379.1991
更新日期:1991-11-01 00:00:00
abstract::The retinoblastoma tumor suppressor protein (pRb) can associate with the transforming proteins of several DNA tumor viruses, including the large T antigen encoded by polyomavirus (Py T Ag). Although pRb function is critical for regulating progression from G1 to S phase, a role for pRb in S phase has not been demonstra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3004-3013.1999
更新日期:1999-04-01 00:00:00
abstract::Phosphonoformate (foscarnet) is a pyrophosphate (PP(i)) analogue and a potent inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT), acting through the PP(i) binding site on the enzyme. HIV-1 RT can unblock a chain-terminated DNA primer by phosphorolytic transfer of the terminal residue t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.11.6127-6137.2003
更新日期:2003-06-01 00:00:00
abstract::Partial deletion of the second hypervariable region from the envelope of the primary-like SF162 virus increases the exposure of certain neutralization epitopes and renders the virus, SF162DeltaV2, highly susceptible to neutralization by clade B and non-clade B human immunodeficiency virus (HIV-positive) sera (L. Stama...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5526-5540.2001
更新日期:2001-06-01 00:00:00
abstract::Heterosubtypic immunity (HSI) is defined as cross-protection to infection with an influenza A virus serotype other than the one used for primary infection. Although HSI has been thought to be mediated by serotype cross-reactive cytotoxic T lymphocytes (CTL) that recognize conserved epitopes of structural proteins, rec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00751-07
更新日期:2007-09-01 00:00:00
abstract::Current L1 virus-like particle (VLP) vaccines provide type-restricted protection against a small subset of the human papillomavirus (HPV) genotypes associated with cervical cancer, necessitating continued cytologic screening of vaccinees. Cervical cancer is most problematic in countries that lack the resources for scr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01577-07
更新日期:2007-11-01 00:00:00
abstract::Host cell-mediated proteolytic cleavage of the human immunodeficiency virus type 1 (HIV-1) gp160 precursor glycoprotein into gp120 and gp41 subunits is required to generate fusion-competent envelope glycoprotein (Env) spikes. The gp120-directed broadly neutralizing monoclonal antibodies (bNabs) isolated from HIV-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01247-13
更新日期:2013-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5297-5305.1996
更新日期:1996-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00167-06
更新日期:2006-06-01 00:00:00
abstract::A member of the family Circoviridae, porcine circovirus type 2 (PCV2), is associated with postweaning multisystemic wasting syndrome (PMWS), a recent emerging disease worldwide. PCV2 is also found in clinically asymptomatic animals. This paradoxical finding makes the syndrome etiology challenging. We developed new ass...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05156-11
更新日期:2011-11-01 00:00:00
abstract::Theiler's virus-induced demyelinating disease results from a chronic infection in the white matter of the central nervous system and provides an excellent model for human multiple sclerosis. Like multiple sclerosis, there are genetic risk factors in disease development, including genes associated with the major histoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.686-690.1990
更新日期:1990-02-01 00:00:00
abstract::Human T-cell lymphotropic/leukemia virus type 1 (HTLV-1) transforms human T cells in vitro, and Tax, a potent transactivator of viral and cellular genes, plays a key role in cell immortalization. Tax activity is mediated by interaction with cellular transcription factors including members of the CREB/ATF family, the N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8852-8860.1998
更新日期:1998-11-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) play an important role in recovery from a number of viral infections. They are also implicated in virus-induced immunopathology as best demonstrated in lymphocytic choriomeningitis virus (LCMV) infection of adult immunocompetent mice. In the present study, the structure of the T-cell rece...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5919-5926.1990
更新日期:1990-12-01 00:00:00
abstract::The simian virus 40 (SV40) large tumor antigen (Tag) is a virus-encoded oncoprotein which is the target of a strong cytotoxic T-lymphocyte (CTL) response. Three immunodominant H-2(b)-restricted epitopes, designated epitopes I, II/III, and IV, have been defined. We investigated whether induction of CTLs directed agains...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5981-5993.1999
更新日期:1999-07-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) tegument is the least understood component of the virion, and the mechanism of tegument assembly and incorporation into virions during viral egress has not yet been elucidated. In the present study, the addition of tegument proteins (VP13/14, VP16, VP22, and US9) and envelope gl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9934-9951.2002
更新日期:2002-10-01 00:00:00
abstract::The 86-kDa IE-2 protein of human cytomegalovirus is able to autoregulate its own expression via a short nucleotide sequence, termed the cis repression signal (CRS), that is located between the TATA box and the cap site of the IE-1/2 enhancer-promoter. Here we report that the 86-kDa IE-2 protein can interact directly w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.323-331.1993
更新日期:1993-01-01 00:00:00
abstract::Wild-type herpes simplex virus 1 (HSV-1) multiplies, spreads, and rapidly destroys cells of the murine central nervous system (CNS). In contrast, mutants lacking both copies of the gamma(1)34.5- gene have been shown to be virtually lacking in virulence even after direct inoculation of high-titered virus into the CNS o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5560-5569.1997
更新日期:1997-07-01 00:00:00
abstract::F proteins from baculovirus nucleopolyhedrovirus (NPV) group II members are the major budded virus (BV) viral envelope fusion proteins. They undergo furin-like proteolysis processing in order to be functional. F proteins from different baculovirus species have a long cytoplasmic tail domain (CTD), ranging from 48 (Spo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01178-06
更新日期:2006-11-01 00:00:00
abstract::CD8(+) cytotoxic T lymphocytes (CTL) play a key role in the control of many virus infections, and the need for vaccines to elicit strong CD8(+) T-cell responses in order to provide optimal protection in such infections is increasingly apparent. However, the mechanisms involved in the induction and maintenance of CD8(+...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7440-7449.1998
更新日期:1998-09-01 00:00:00
abstract::The cytoplasmic tail (R peptide) sequence is able to regulate the fusion activity of the murine leukemia virus (MuLV) envelope (Env) protein. We have previously shown that this sequence exerts a profound inhibitory effect on the fusion activity of simian immunodeficiency virus (SIV)-MuLV chimeric Env proteins which co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.6217-6222.2000
更新日期:2000-07-01 00:00:00
abstract::The U(S)3 open reading frame of herpes simplex virus 1 (HSV-1) was reported to encode two mRNAs each directing the synthesis of the same protein. We report that the U(S)3 gene encodes two proteins. The predominant U(S)3 protein is made in wild-type HSV-1-infected cells. The truncated mRNA and a truncated protein desig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8470-8479.2005
更新日期:2005-07-01 00:00:00
abstract::All presently available replication-competent proviral clones of human immunodeficiency virus type 1 (HIV-1) are derived from cell culture-amplified virus. Since tissue culture is highly selective for viral strains with an in vitro growth advantage, such clones may not be representative of the biologically relevant vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.3973-3985.1991
更新日期:1991-08-01 00:00:00
abstract::Attempts to elicit broadly neutralizing antibody responses by human immunodeficiency virus type 1 (HIV-1) vaccine antigens have been met with limited success. To better understand the requirements for cross-neutralization of HIV-1, we have characterized the neutralizing antibody specificities present in the sera of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02749-06
更新日期:2007-06-01 00:00:00
abstract::The chimpanzee is the only animal model for investigating the pathogenesis of viral hepatitis types A through E in humans. Studies of the host response, including microarray analyses, have relied on the close relationship between these two primate species: chimpanzee samples are commonly tested with human-based reagen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01205-10
更新日期:2010-11-01 00:00:00
abstract::Human cytomegalovirus (HCMV) encodes four putative G protein-coupled receptors, including pUL78, whose rodent orthologues are known to be important for replication and spread in their hosts. To investigate the mechanism by which pUL78 contributes to viral replication and pathogenesis, we generated a derivative of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05900-11
更新日期:2012-11-01 00:00:00
abstract:UNLABELLED:Nonhuman primates (NHPs) are a historically important source of zoonotic viruses and are a gold-standard model for research on many human pathogens. However, with the exception of simian immunodeficiency virus (SIV) (family Retroviridae), the blood-borne viruses harbored by these animals in the wild remain i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00573-16
更新日期:2016-07-11 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6653-6657.1996
更新日期:1996-10-01 00:00:00
abstract::We have obtained a polyclonal antiserum, N-BE, against the denatured, amino-terminal half of the measles virus (MV) nucleocapsid (N) protein and a monoclonal antibody (MAb), N46, which recognizes a conformation-dependent epitope in the same region. Amino acid residues 23 to 239 were required and sufficient for the for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4133-4141.1993
更新日期:1993-07-01 00:00:00
abstract::Subgenomic RNAs of both polarities corresponding to rice hoja blanca virus (RHBV) ambisense RNA4 were detected in RHBV-infected rice tissues. Total RNA extracted from RHBV-infected and noninfected rice tissues and RNA4 purified from RHBV ribonucleoprotein particles were used as templates for primer extension studies. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1951-1954.1995
更新日期:1995-03-01 00:00:00