Abstract:
:The vif gene of human immunodeficiency virus type 1 is absolutely required for productive infection of primary cells derived from human blood and certain immortalized T lymphoid cells, for example, H9. Cells with this restrictive phenotype are termed nonpermissive, whereas cell lines in which vif-deficient virus can replicate efficiently are known as permissive. In this paper, we describe experiments in which virus stocks derived from single-cycle infections of strictly nonpermissive H9 cells were used to determine the fate of vif-deficient infections. By PCR-based approaches, it was found that Vif has no significant impact on the biosynthetic capability of the virion reverse transcriptase in infected C8166 T cells. Specifically, the initial appearance of all DNA species up to and including initiated second (plus) strands as well as the early accumulation of these replicative intermediates is equivalent for wild-type and vif-deficient infections. However, whereas these viral DNAs are stably maintained in wild-type infections and can proceed to establish proviruses, they are largely degraded by the later time points of vif-deficient infections and, as a result, are prevented from forming proviruses. Subcellular fractionation analyses indicated that the majority of viral DNA is localized to the nucleus within 2 h of infection and that the turnover of reverse transcripts that occurs in these vif-deficient infections presumably takes place in the nucleus. Given that the ultimate infection phenotype of the virions is determined during virus production, we propose that Vif is required for an aspect of virus assembly and/or maturation that endows penetrating viral nucleoprotein cores with the ability to mature into functional preintegration complexes that can proceed to provirus establishment. In contrast, viruses that are produced in the absence of Vif give rise to nucleoprotein complexes that disassemble prematurely in challenged cells and fail to protect their RNA/DNA contents from nucleolytic destruction.
journal_name
J Viroljournal_title
Journal of virologyauthors
Simon JH,Malim MHdoi
10.1128/JVI.70.8.5297-5305.1996subject
Has Abstractpub_date
1996-08-01 00:00:00pages
5297-305issue
8eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::Temperature-sensitive COS cells, clone E540, have been stably transformed at a restrictive temperature with plasmid pVA1, which contains the adenovirus type 5 virus-associated (VA) genes in addition to the Neor marker. Transformed cell clones, named EVA cells, contained adenovirus DNA in an integrated form while grown...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5445-5450.1989
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abstract::Nipah virus (NiV), of the family Paramyxoviridae, was isolated in 1999 in Malaysia from a human fatality in an outbreak of severe human encephalitis, when human infections were linked to transmission of the virus from pigs. Consequently, a swine vaccine able to abolish virus shedding is of veterinary and human health ...
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doi:10.1128/JVI.00263-06
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doi:10.1128/JVI.24.2.541-550.1977
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00717-09
更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.00935-14
更新日期:2014-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.26-35.2001
更新日期:2001-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3661-3668.1989
更新日期:1989-09-01 00:00:00
abstract::Retroviral vectors offer major advantages for gene transfer studies but have not been useful for producing proteins in large quantities. This deficiency has resulted in part from interference to superinfection, which limits the numbers of active proviruses in cells. Recently, we found that these vectors amplify when t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.7.3500-3508.1990
更新日期:1990-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8303-8307.1999
更新日期:1999-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2543-2549.1989
更新日期:1989-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6540-6550.1999
更新日期:1999-08-01 00:00:00
abstract:UNLABELLED:Hepadnaviruses (hepatitis B viruses [HBVs]) are the only animal viruses that replicate their DNA by reverse transcription of an RNA intermediate. Until recently, the known host range of hepadnaviruses was limited to mammals and birds. We obtained and analyzed the first amphibian HBV genome, as well as severa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00832-16
更新日期:2016-08-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.1.63-72.1979
更新日期:1979-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9260-9270.2002
更新日期:2002-09-01 00:00:00
abstract::Mouse mammary tumor virus (MMTV)-induced mammary adenocarcinomas can develop from several different premalignant precursors common in GR mice. Insertion mutagenesis of the mammary protooncogenes int-1 and int-2 was studied in this multistep system by analyzing samples from various stages of neoplastic development for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.4.1794-1802.1990
更新日期:1990-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.151-157.1998
更新日期:1998-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00109-11
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3235-3240.1998
更新日期:1998-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8669-8676.1999
更新日期:1999-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.3.621-629.1979
更新日期:1979-09-01 00:00:00
abstract::An immediate-early protein of murine cytomegalovirus (MCMV), pp89, elicits an immunodominant and protective major histocompatibility complex (MHC) class I Ld-restricted CD8+ T-lymphocyte response. Remarkably, presentation of the naturally processed peptide of pp89, the nonapeptide YPHFMPTNL, is abolished during permis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.289-297.1994
更新日期:1994-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.175-186.1979
更新日期:1979-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4407-4414.2005
更新日期:2005-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01242-09
更新日期:2009-11-01 00:00:00
abstract::A virus recovered from the saliva of a child with chronic active Epstein-Barr virus (EBV) infection for 8 years was shown to induce EBV early antigen (EBV-EA) in Raji cells and to be expressed into EBV-EA in fresh EBV-negative peripheral blood leukocytes. However, it did not replicate its DNA. Oropharyngeal epithelial...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3306-3309.1987
更新日期:1987-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3752-3760.2000
更新日期:2000-04-01 00:00:00
abstract::Open reading frame 24 (ORF24) of murine gammaherpesvirus 68 (MHV-68) is conserved among beta- and gammaherpesviruses; however, its function in viral replication has not been defined. Using MHV-68 as a model, we have identified ORF24 as being essential for viral replication. An ORF24-null virus was generated and shown ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02726-06
更新日期:2007-06-01 00:00:00