Abstract:
:The hemagglutinin-neuraminidase (HN) protein of paramyxoviruses carries out three discrete activities that each affect the ability of HN to promote viral fusion and entry: receptor binding, receptor cleaving (neuraminidase), and triggering of the fusion protein. The interrelationship between the receptor binding and fusion-triggering functions of HN has not been clear. For human parainfluenza type 3 (HPIV3), one bifunctional site on HN can carry out both receptor binding and neuraminidase activities, and this site's receptor binding can be inhibited by the small receptor analog zanamivir. We now report experimental evidence, complemented by computational data, for a second receptor binding site near the HPIV3 HN dimer interface. This second binding site can mediate receptor binding even in the presence of zanamivir, and it differs from the second receptor binding site of the paramyxovirus Newcastle disease virus in its function and its relationship to the primary binding site. This second binding site of HPIV3 HN is involved in triggering F. We suggest that the two receptor binding sites on HPIV3 HN each contribute in distinct ways to virus-cell interaction; one is the multifunctional site that contains both binding and neuraminidase activities, and the other contains binding activity and also is involved in fusion promotion.
journal_name
J Viroljournal_title
Journal of virologyauthors
Porotto M,Fornabaio M,Kellogg GE,Moscona Adoi
10.1128/JVI.02617-06subject
Has Abstractpub_date
2007-04-01 00:00:00pages
3216-28issue
7eissn
0022-538Xissn
1098-5514pii
JVI.02617-06journal_volume
81pub_type
杂志文章abstract::Prior studies have demonstrated that a small proportion of blood lymphocytes from patients with human cytomegalovirus (HCMV) infection express only the viral immediate-early (IE) genes (L. Einhorn and A. Ost, J. Infect. Dis. 149:207-214, 1984; G. P. A. Rice, R. D. Schrier, and M. B. A. Oldstone, Proc. Natl. Acad. Sci....
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doi:10.1128/JVI.63.7.3026-3033.1989
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doi:10.1128/JVI.73.3.2365-2375.1999
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pub_type: 杂志文章
doi:10.1128/JVI.56.2.607-612.1985
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5505-5508.1989
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doi:10.1128/JVI.78.21.11591-11604.2004
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3832-3840.1987
更新日期:1987-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.626-628.1983
更新日期:1983-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.3.860-871.1980
更新日期:1980-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.7810-7815.1994
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1974-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.5.617-623.1970
更新日期:1970-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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