Selection of revertants of Kirsten sarcoma virus transformed nonproducer BALB-3T3 cells.

Abstract:

:Revertants of Kirsten sarcoma virus transformed nonproducer BALB/3T3 cells (KA31 cells) were isolated after exposing the transformed cells to 5-fluorodeoxyuridine at high cell density, or when suspended in methylcellulose. Revertants were also isolated by treating KA31 cells with the lectin, concanavalin A, which is manyfold more toxic to transformed cells than for normal cells. The revertants resemble BALB/3T3 cells in their morphology and growth characteristics in that they have a low saturation density, fail to grow in 1% calf serum or when suspended in methylcellulose, and cease to synthesize DNA after reaching their saturation density. Infection by murine leukemia virus rescues Kirsten sarcoma virus from only the concanavalin-A-selected variants, though all the revertants are susceptible to infection by leukemia virus. The concanavalin A revertants also become transformed after infection with murine leukemia virus. All the revertants can be transformed by Kirsten sarcoma virus but not by simian virus 40.

journal_name

J Virol

journal_title

Journal of virology

authors

Ozanne B,Vogel A

doi

10.1128/JVI.14.2.239-248.1974

subject

Has Abstract

pub_date

1974-08-01 00:00:00

pages

239-48

issue

2

eissn

0022-538X

issn

1098-5514

journal_volume

14

pub_type

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