Abstract:
:Live-attenuated vesicular stomatitis virus (VSV) vectors expressing foreign antigens induce potent immune responses and protect against viral diseases in animal models. Highly attenuated (VSV-CT1) or single-cycle VSV (VSVDeltaG) vectors induce immune responses lower than those generated by attenuated wild-type VSV vectors when given intranasally. We show here that reduced spread of the more highly attenuated or single-cycle vectors to other organs, including lymph nodes, correlates with the reduction in the immune responses. A reverse transcription, real-time PCR assay for VSV genomic RNA (gRNA) sequences showed long-term persistence of gRNA from replicating vectors in lymph nodes, long after viral clearance. Such persistence may be important for induction of potent immune responses by VSV vectors.
journal_name
J Viroljournal_title
Journal of virologyauthors
Simon ID,Publicover J,Rose JKdoi
10.1128/JVI.02525-06subject
Has Abstractpub_date
2007-02-01 00:00:00pages
2078-82issue
4eissn
0022-538Xissn
1098-5514pii
JVI.02525-06journal_volume
81pub_type
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