Mutations in classical swine fever virus NS4B affect virulence in swine.

abstract：:To gain further insight on the function of the herpes simplex virus type 1 (HSV-1) packaging signal (a sequence), we constructed a recombinant virus containing a unique a sequence, which was flanked by two loxP sites in parallel orientation. The phenotype of this recombinant, named HSV-1 LaL, was studied in cell lines...

journal_title：Journal of virology

authors： Logvinoff C,Epstein AL

更新日期：2000-09-01 00:00:00

abstract：:The envelope glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV) is posttranslationally cleaved into two subunits. We show here that this endoproteolytic processing is not required for transport to the cell surface but is essential for LCMV GP to mediate infectivity of pseudotyped retroviral vectors. By sys...

journal_title：Journal of virology

authors： Beyer WR,Pöpplau D,Garten W,von Laer D,Lenz O

更新日期：2003-03-01 00:00:00

abstract：:Although recombinant human adenovirus (HAV)-based vectors offer several advantages for somatic gene therapy and vaccination over other viral vectors, it would be desirable to develop alternative vectors with prolonged expression and decreased toxicity. Toward this objective, a replication-defective bovine adenovirus t...

journal_title：Journal of virology

authors： Reddy PS,Idamakanti N,Chen Y,Whale T,Babiuk LA,Mehtali M,Tikoo SK

更新日期：1999-11-01 00:00:00

abstract：:The 5' untranslated region (UTR) of hepatitis C virus (HCV), which is composed of four domains (I, II, III, and IV) and a pseudoknot, is essential for translation and viral replication. Equine nonprimate hepacivirus (EHcV) harbors a 5' UTR consisting of a large 5'-terminal domain (I); three additional domains (I', II,...

journal_title：Journal of virology

authors： Tanaka T,Otoguro T,Yamashita A,Kasai H,Fukuhara T,Matsuura Y,Moriishi K

更新日期：2018-03-14 00:00:00

abstract：:We have developed a reconstituted system which models the events associated with human immunodeficiency virus type 1 (HIV-1) plus-strand transfer. These events include synthesis of plus-strand strong-stop DNA [(+) SSDNA] from a minus-strand DNA donor template covalently attached to human tRNA3Lys, tRNA primer removal,...

journal_title：Journal of virology

authors： Wu T,Guo J,Bess J,Henderson LE,Levin JG

更新日期：1999-06-01 00:00:00

abstract：:Oral mucosa is one of the main target tissues of the human pathogen herpes simplex virus 1 (HSV-1). How the virus overcomes the protective epithelial barriers and penetrates the tissue to reach its receptors and initiate infection is still unclear. Here, we established an ex vivo infection assay with human oral mucosa...

journal_title：Journal of virology

authors： Thier K,Petermann P,Rahn E,Rothamel D,Bloch W,Knebel-Mörsdorf D

更新日期：2017-10-27 00:00:00

abstract：:The RNA-dependent RNA polymerase of influenza A virus is composed of three subunits that together synthesize all viral mRNAs and also replicate the viral genomic RNA segments (vRNAs) through intermediates known as cRNAs. Here we describe functional characterization of 16 site-directed mutants of one polymerase subunit...

journal_title：Journal of virology

authors： Regan JF,Liang Y,Parslow TG

更新日期：2006-01-01 00:00:00

abstract：:The potential role of T cells in the control of human papillomavirus type 6 (HPV-6) infections is an appealing premise, but their actual role has been sparsely investigated. Since HPV-6 infections are confined to the epithelium, such an investigation should focus on the T cells present at the site of infection (i.e., ...

journal_title：Journal of virology

authors： Hong K,Greer CE,Ketter N,Van Nest G,Paliard X

更新日期：1997-09-01 00:00:00

abstract：:ICP0, one of the five immediate-early (IE) gene products of herpes simplex virus (HSV), is a potent activator of transcription. To assess the biological activities of ICP0 and to explore its mechanisms of action, two helper-independent recombinant adenoviruses were constructed. In each recombinant, the E1 region was s...

journal_title：Journal of virology

authors： Zhu XX,Young CS,Silverstein S

更新日期：1988-12-01 00:00:00

abstract：UNLABELLED:Hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) are critical in eliminating infection. We developed an animal model in which HBV-infected human hepatocytes are targeted by HBV-specific CTLs. After HBV inoculation in human hepatocyte-transplanted herpes simplex virus type-1 thymidine kinase-NO...

journal_title：Journal of virology

authors： Uchida T,Hiraga N,Imamura M,Tsuge M,Abe H,Hayes CN,Aikata H,Ishida Y,Tateno C,Yoshizato K,Ohdan H,Murakami K,Chayama K

更新日期：2015-10-01 00:00:00

abstract：:Using isogenic recombinant murine coronaviruses expressing wild-type murine hepatitis virus strain 4 (MHV-4) or MHV-A59 spike glycoproteins or chimeric MHV-4/MHV-A59 spike glycoproteins, we have demonstrated the biological functionality of the N-terminus of the spike, encompassing the receptor binding domain (RBD). We...

journal_title：Journal of virology

authors： Tsai JC,Zelus BD,Holmes KV,Weiss SR

更新日期：2003-01-01 00:00:00

abstract：:LA3382 is a temperature-sensitive replication-defective mutant of Rous sarcoma virus that contains four active mutations. In this study we performed experiments to determine the biochemical defect that blocks the synthesis of infections virus late in the replication cycle. At the nonpermissive temperature (41 degrees ...

journal_title：Journal of virology

authors： Hardwick JM,Hunter E

更新日期：1981-12-01 00:00:00

abstract：UNLABELLED:Lifelong antiretroviral therapy (ART) for HIV-1 does not diminish the established latent reservoir. A possible cure approach is to reactivate the quiescent genome from latency and utilize immune responses to eliminate cells harboring reactivated HIV-1. It is not known whether antibodies within HIV-1-infected...

journal_title：Journal of virology

authors： Lee WS,Richard J,Lichtfuss M,Smith AB 3rd,Park J,Courter JR,Melillo BN,Sodroski JG,Kaufmann DE,Finzi A,Parsons MS,Kent SJ

更新日期：2015-12-09 00:00:00

abstract：:A porcine rotavirus (prv), variant 4F, isolated in tissue culture from the feces of a Chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (J. C. Bridger, B. Burke, G. M. Beards, and U. Desselberger, J. Gen. Virol. 73:3011-3015, 1992). Comparison with the closely r...

journal_title：Journal of virology

authors： Tauscher GI,Desselberger U

更新日期：1997-01-01 00:00:00

abstract：:We established a system for propagation of Sindbis virus (SIN)-based replicons in tissue culture in the form of a tricomponent genome virus. Three RNA fragments containing complementing genetic information required for virus replication are packaged into separate viral particles, and each cell produces at least 1,000 ...

journal_title：Journal of virology

authors： Fayzulin R,Gorchakov R,Petrakova O,Volkova E,Frolov I

更新日期：2005-01-01 00:00:00

abstract：:Bovine spongiform encephalopathy (BSE) is a fatal, transmissible, neurodegenerative disease of cattle. BSE can be transmitted experimentally between cattle through the oral route, and in this study, brain tissue samples from animals at different time points postinoculation were analyzed for changes in gene expression....

journal_title：Journal of virology

authors： Tang Y,Xiang W,Hawkins SA,Kretzschmar HA,Windl O

更新日期：2009-09-01 00:00:00

abstract：:Experimental evidence from the simian immunodeficiency virus (SIV) model of AIDS has shown that the nef gene is critical in the pathogenesis of AIDS. Consequently, nef is of considerable interest in both antiviral drug and vaccine development. Preliminary findings in two rhesus macaques indicated that a deletion of on...

journal_title：Journal of virology

authors： Whatmore AM,Cook N,Hall GA,Sharpe S,Rud EW,Cranage MP

更新日期：1995-08-01 00:00:00

abstract：:Poorly cytopathic or noncytopathic viruses can escape immune surveillance and establish a chronic infection. Here we exploited the strategy of combining antiviral drug treatment with the induction of a neutralizing antibody response to avoid the appearance of neutralization-resistant virus variants. Despite the fact t...

journal_title：Journal of virology

authors： Seiler P,Senn BM,Klenerman P,Kalinke U,Hengartner H,Zinkernagel RM

更新日期：2000-07-01 00:00:00

abstract：:Ganglia of monkeys with reactivated simian varicella virus (SVV) contained more CD8 than CD4 T cells around neurons. The abundance of CD8 T cells was greater less than 2 months after reactivation than that at later times and correlated with that of CXCL10 RNA but not with those of SVV protein or open reading frame 61 ...

journal_title：Journal of virology

authors： Ouwendijk WJ,Abendroth A,Traina-Dorge V,Getu S,Steain M,Wellish M,Andeweg AC,Osterhaus AD,Gilden D,Verjans GM,Mahalingam R

更新日期：2013-03-01 00:00:00

abstract：:Viruses encode proteins that disrupt chemokine responses. The murine gammaherpesvirus 68 gene M3 encodes a chemokine binding protein (vCKBP-3) which has no sequence similarity to chemokine receptors but inhibits chemokine receptor binding and activity. We have used a panel of CXCL8 analogs to identify the structural r...

journal_title：Journal of virology

authors： Webb LM,Clark-Lewis I,Alcami A

更新日期：2003-08-01 00:00:00

abstract：:The nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, Ad2(+)ND(1), differs from the defective Ad-SV40 hybrid populations previously described, in that this hybrid virus can replicate without the aid of nonhybrid adenovirus helper. Consequently, the deoxyribonucleic acid (DNA) from this virus, which ...

journal_title：Journal of virology

authors： Crumpacker CS,Henry PH,Kakefuda T,Rowe WP,Levin MJ,Lewis AM Jr

更新日期：1971-03-01 00:00:00

abstract：:Respiratory syncytial (RS) virus infects the epithelium of the respiratory tract. We examined the replication and maturation of RS virus in two polarized epithelial cell lines, Vero C1008 and MDCK. Electron microscopy of RS virus-infected Vero C1008 cells revealed the presence of pleomorphic viral particles budding ex...

journal_title：Journal of virology

authors： Roberts SR,Compans RW,Wertz GW

更新日期：1995-04-01 00:00:00

abstract：:Several reports have shown that human cytomegalovirus (HCMV)-infected cells are resistant to NK lysis. These studies have focused on receptor-ligand interactions, and different HCMV proteins have been indicated to mediate inhibitory NK signals. Here, we report that the HCMV protein UL16 is of major importance for the ...

journal_title：Journal of virology

authors： Odeberg J,Browne H,Metkar S,Froelich CJ,Brandén L,Cosman D,Söderberg-Nauclér C

更新日期：2003-04-01 00:00:00

abstract：:We tested the ability of human monoclonal antibodies (immunoglobulin G1b12 [IgG1b12] and 19b) and CD4-based molecules (CD4-IgG2 and soluble CD4 [sCD4]) to neutralize human immunodeficiency virus type 1 directly from the plasma of seropositive donors in an ex vivo neutralization assay. IgG1b12 and CD4-IgG2, at concentr...

journal_title：Journal of virology

authors： Gauduin MC,Allaway GP,Maddon PJ,Barbas CF 3rd,Burton DR,Koup RA

更新日期：1996-04-01 00:00:00

abstract：:Protein synthesis in adenovirus-infected cells is regulated during the late phase of infection. The rate of initiation is maintained by a small viral RNA, virus-associated (VA) RNAI, which prevents the phosphorylation of eukaryotic initiation factor eIF-2 by a double-stranded RNA-activated protein kinase, DAI. On the ...

journal_title：Journal of virology

authors： Pe'ery T,Mellits KH,Mathews MB

更新日期：1993-06-01 00:00:00

abstract：:In 1997, an H5N1 influenza virus outbreak occurred in chickens in Hong Kong, and the virus was transmitted directly to humans. Because there is limited information about the avian influenza virus reservoir in that region, we genetically characterized virus strains isolated in Hong Kong during the 1997 outbreak. We seq...

journal_title：Journal of virology

authors： Hoffmann E,Stech J,Leneva I,Krauss S,Scholtissek C,Chin PS,Peiris M,Shortridge KF,Webster RG

更新日期：2000-07-01 00:00:00

abstract：:The RNA genome of human immunodeficiency virus type 1 (HIV-1) is converted into DNA after infection in order to integrate into the host cell DNA. However, a large number of these reverse-transcribed genomes remain unintegrated in the nucleus of infected cells. Currently, there are no data available about the intranucl...

journal_title：Journal of virology

authors： Bell P,Montaner LJ,Maul GG

更新日期：2001-08-01 00:00:00

abstract：:Nonstructural protein 4 (nsp4; 204 amino acids) is the chymotrypsin-like serine main proteinase of the arterivirus Equine arteritis virus (order Nidovirales), which controls the maturation of the replicase complex. nsp4 includes a unique C-terminal domain (CTD) connected to the catalytic two-beta-barrel structure by t...

journal_title：Journal of virology

authors： van Aken D,Snijder EJ,Gorbalenya AE

更新日期：2006-04-01 00:00:00

abstract：:Cytotoxic-T-lymphocyte (CTL) escape mutations undermine the durability of effective human immunodeficiency virus type 1 (HIV-1)-specific CD8(+) T cell responses. The rate of CTL escape from a given response is largely governed by the net of all escape-associated viral fitness costs and benefits. The observation that C...

journal_title：Journal of virology

authors： Boutwell CL,Carlson JM,Lin TH,Seese A,Power KA,Peng J,Tang Y,Brumme ZL,Heckerman D,Schneidewind A,Allen TM

更新日期：2013-04-01 00:00:00

abstract：:Individuals infected with the human immunodeficiency virus (HIV) experience a marked loss of CD4+ T lymphocytes, leading to fatal immunodeficiency. The mechanisms causing the depletion of these cells are not yet understood. In this study, we observed that CD4+ T lymphocytes from HIV type 1 (HIV-1)-infected and uninfec...

journal_title：Journal of virology

authors： Heinkelein M,Sopper S,Jassoy C

更新日期：1995-11-01 00:00:00