Abstract:
:ICP0, one of the five immediate-early (IE) gene products of herpes simplex virus (HSV), is a potent activator of transcription. To assess the biological activities of ICP0 and to explore its mechanisms of action, two helper-independent recombinant adenoviruses were constructed. In each recombinant, the E1 region was substituted with the ICP0-encoding genomic segment under the control of either the adenovirus major late promoter (MLP-0) or the HSV IE-0 promoter (0PRO-0). Infection of HeLa cells or 293 cells (a human embryo kidney cell line expressing adenovirus 5 E1a and -b functions) with the MLP-0 recombinant results in the synthesis of more IE-0 mRNA and ICP0 protein than did infection with the 0PRO-0 recombinant. Although 293 cells infected with MLP-0 accumulate 5- to 10-fold more IE-0 mRNA late in the infection than cells infected with HSV, the level of the protein product, ICP0, increased only slightly. In 293 cells, both recombinants could replicate, albeit at a slower rate and with lower final yields than wild-type adenovirus. Neither virus replicates its DNA in HeLa cells, and thus ICP0 cannot substitute for adenovirus E1a; however, the level of ICP0 that accumulates in MLP-0-infected HeLa cells was comparable to that of HSV-infected HeLa cells. In a functional test, we demonstrated that the adeno-ICP0 recombinant viruses can transactivate a transfected TK-CAT cassette, indicating that the ICP0 is biologically active.
journal_name
J Viroljournal_title
Journal of virologyauthors
Zhu XX,Young CS,Silverstein Sdoi
10.1128/JVI.62.12.4544-4553.1988subject
Has Abstractpub_date
1988-12-01 00:00:00pages
4544-53issue
12eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::Wild-type simian virus 40 A protein (large T antigen) bound to three tandem regions of simian virus 40 DNA. The binding regions were defined by the ability of A protein to protect simian virus 40 DNA from digestion with limited (footprint assay) or excess (fragment assay) amounts of DNase I. At low concentrations, pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.458-466.1982
更新日期:1982-11-01 00:00:00
abstract::The complete nucleotide sequences of the vesicular stomatitis virus mRNA's encoding the glycoprotein (G) and the matrix protein (M) have been determined from cDNA clones that contain the complete coding sequences from each mRNA. The G protein mRNA is 1,665 nucleotides long, excluding polyadenylic acid, and encodes a p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.519-528.1981
更新日期:1981-08-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.00202-08
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abstract::Most retroviral packaging cell lines were established by a helper virus plasmid cotransfected with a separate plasmid encoding a selection marker. Since this selection marker coexisted in trans with the helper virus sequence, helper virus gene expression could be inactivated by host DNA methylation despite selection f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5242-5249.2000
更新日期:2000-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.4.1996-2001.2001
更新日期:2001-02-01 00:00:00
abstract::In immunocompromised patients, infection with Kaposi's sarcoma-associated herpesvirus (KSHV) can give rise to Kaposi's sarcoma and several lymphoproliferative disorders. In these tumors, KSHV establishes a latent infection in many of the rapidly proliferating and morphologically abnormal cells. Only a few viral gene p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.10074-10085.2004
更新日期:2004-09-01 00:00:00
abstract::An in vitro assay was developed to investigate endonuclease activity of Thogoto virus, a tick-borne orthomyxovirus. Endonuclease activity relied on an interaction between the 3' and 5' termini of virion RNA (vRNA) and not those of cRNA. Evidence was obtained that cap structures are cleaved directly from cap donors and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2305-2309.1998
更新日期:1998-03-01 00:00:00
abstract::The 104-S monoclonal antibody immunoprecipitated from herpes simplex virus type 2 (HSV-2)-infected cell extracts the 75,000-molecular-weight glycoprotein gF and its 65,000-molecular-weight precursor (pgF). The precursor pgF was sensitive to endoglycosidase H digestion, indicating the presence of high mannose-type olig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.1.185-192.1983
更新日期:1983-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03120-15
更新日期:2016-03-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03659-14
更新日期:2015-04-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) capsid is a T=16 icosahedral shell that forms in the nuclei of infected cells. Capsid assembly also occurs in vitro in reaction mixtures created from insect cell extracts containing recombinant baculovirus-expressed HSV-1 capsid proteins. During capsid formation, the major capsi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3944-3951.1998
更新日期:1998-05-01 00:00:00
abstract::Current L1 virus-like particle (VLP) vaccines provide type-restricted protection against a small subset of the human papillomavirus (HPV) genotypes associated with cervical cancer, necessitating continued cytologic screening of vaccinees. Cervical cancer is most problematic in countries that lack the resources for scr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01577-07
更新日期:2007-11-01 00:00:00
abstract::Deer mice are the principal reservoir hosts of Sin Nombre virus, the etiologic agent of most hantavirus cardiopulmonary syndrome cases in North America. Infection of deer mice results in persistence without conspicuous pathology, and most, if not all, infected mice remain infected for life, with periods of viral shedd...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06875-11
更新日期:2012-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.8.4145-4155.2004
更新日期:2004-04-01 00:00:00
abstract::Numerous deaths of koi and common carp (Cyprinus carpio) were observed on many farms throughout Israel, resulting in severe financial losses. The lethal viral disease observed is highly contagious and extremely virulent, but morbidity and mortality are restricted to koi and common carp populations. Diseased fish exhib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.1983-1991.2005
更新日期:2005-02-01 00:00:00
abstract::We have examined the relative ability of Sendai virus M (matrix) protein to associate with membranes containing viral glycoproteins at three distinct stages of the exocytic pathway prior to cell surface appearance. By the use of selective low-temperature incubations or the ionophore monensin, the transport of newly sy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.651-663.1993
更新日期:1993-02-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) infects alveolar macrophages (AMϕ), causing dysregulated alpha interferon (IFN-α) and tumor necrosis factor alpha (TNF-α) production through a mechanism(s) yet to be resolved. Here, we show that AMϕ infected with PRRSV secreted a reduced quantity of IFN-α fol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01251-17
更新日期:2018-01-02 00:00:00
abstract::Unintegrated linear and closed circular DNAs of B- and N-tropic endogenous BALB/c murine leukemia virus (MuLV) were extracted from newly infected mouse cells and cleaved with EcoRI, XhoI, PvuI, HindIII, SalI, XbaI, KpnI, SmaI, and PstI restriction endonucleases. The DNA fragments were separated by electrophoresis and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.812-823.1980
更新日期:1980-09-01 00:00:00
abstract::Sequences of the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) domain were determined by direct sequencing of HIV-1 RNA in successive plasma samples from eight seroconverting patients infected with virus bearing the T215Y/F amino acid substitution associated with zidovudine (ZDV) resistance. A...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3520-3523.1998
更新日期:1998-05-01 00:00:00
abstract::Plasma human immunodeficiency virus type 1 (HIV-1) turnover and kinetics were studied in children aged 15 days to 2 years following the initiation of a triple antiretroviral drug regimen consisting of zidovudine, lamivudine, and nevirapine. HIV-1 turnover was at least as rapid as that previously described in adults; t...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.73.1.362-367.1999
更新日期:1999-01-01 00:00:00
abstract:UNLABELLED:PB1-F2 protein, the 11th influenza A virus (IAV) protein, is considered to play an important role in primary influenza virus infection and postinfluenza secondary bacterial pneumonia in mice. The functional role of PB1-F2 has been reported to be a strain-specific and host-specific phenomenon. Its precise con...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01551-15
更新日期:2015-10-14 00:00:00
abstract::Human T-cell leukemia virus type I (HTLV-I)-infected T-cell lines constitutively produce high levels of biologically active lymphotoxin (LT; tumor necrosis factor-beta) protein and LT mRNA. To understand the regulation of LT transcription by HTLV-I, we analyzed the ability of a series of deletions of the LT promoter t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5412-5419.1990
更新日期:1990-11-01 00:00:00
abstract::Rabies virus (RV) of the Rhabdoviridae family grows in alpha/beta interferon (IFN)-competent cells, suggesting the existence of viral mechanisms preventing IFN gene expression. We here identify the viral phosphoprotein P as the responsible IFN antagonist. The critical involvement of P was first suggested by the observ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7673-7681.2005
更新日期:2005-06-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) entry into target cells requires folding of two heptad-repeat regions (N-HR and C-HR) of gp41 into a trimer of N-HR and C-HR hairpins, which brings viral and target cell membranes together to facilitate membrane fusion. Peptides corresponding to the N-HR and C-HR of gp41 are...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.1666-1671.2003
更新日期:2003-02-01 00:00:00
abstract::P-element-induced wimpy-like (Piwil) proteins restrict the replication of mobile genetic elements in the germ line. They are also expressed in many transformed cell lines. In this study, we discovered that the human Piwil 2 (Hili) protein can also inhibit HIV replication, especially in activated CD4+ T cells that are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00237-17
更新日期:2017-05-12 00:00:00
abstract::Measles is a highly contagious human disease caused by the measles virus (MeV). In this study, by proteomic analysis, we identified peroxiredoxin 1 (Prdx1) as a host factor that binds to the C-terminal region of the nucleoprotein (N; N(TAIL)) of MeV. Glutathione S-transferase (GST) pulldown experiments showed that the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01796-10
更新日期:2011-03-01 00:00:00
abstract::The RNA produced in vivo from bacteriophage phiX174 DNA has been analyzed by polyacrylamide-agarose gel electrophoresis and sedimentation in dimethyl sulfoxide gradients, and the results of Hayashi and Hayashi (1970) have been confirmed and extended. An efficient procedure for recovery of RNA from gels, followed by a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.151-160.1975
更新日期:1975-01-01 00:00:00
abstract::Our previous observations indicated that upon infection with minute virus of mice (MVM), Ehrlich ascites cells lose a transcription elongation activity which is essential for the readthrough of the MVM attenuator. This was monitored by the ability of extracts from uninfected but not from infected cells to support read...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2741-2745.1994
更新日期:1994-04-01 00:00:00
abstract::Retroviral genomes are dimeric, comprised of two sense-strand RNAs linked at their 5' ends by noncovalent base pairing and tertiary interactions. Viral maturation involves large-scale morphological changes in viral proteins and in genomic RNA dimer structures to yield infectious virions. Structural studies have largel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01602-09
更新日期:2010-01-01 00:00:00
abstract::In some retroviruses, such as Rous sarcoma virus and prototype foamy virus, Gag proteins are known to shuttle between the nucleus and the cytoplasm and are implicated in nuclear export of the viral genomic unspliced RNA (gRNA) for subsequent encapsidation. A similar function has been proposed for human immunodeficienc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06874-11
更新日期:2012-03-01 00:00:00