Isolation and characterization of human papillomavirus type 6-specific T cells infiltrating genital warts.

Abstract:

:The potential role of T cells in the control of human papillomavirus type 6 (HPV-6) infections is an appealing premise, but their actual role has been sparsely investigated. Since HPV-6 infections are confined to the epithelium, such an investigation should focus on the T cells present at the site of infection (i.e., the warts). Therefore, we isolated wart-infiltrating lymphocytes (WIL) from patients with clinically diagnosed anogenital warts. These WIL were characterized by their phenotype and their specificity for E7 and L1 proteins of HPV-6. The phenotype of WIL varied drastically from patient to patient, as determined by their expression of CD4, CD8, T-cell receptor alpha/beta chain (TCR alpha beta), and TCR gamma delta. Despite this heterogeneity in phenotype, HPV-6 E7 and/or L1-specific WIL, as determined by lymphoproliferation, could be isolated from more than 75% of the patients studied. Among all L1 peptides recognized by WIL, peptides 311-330 and 411-430 were the most consistently detected, with seven of nine patients for whom L1 peptide reactivity was observed responding to at least one of them. Moreover, the HPV-6 epitopic peptides recognized by WIL differed to some extent from those recognized by peripheral T cells.

journal_name

J Virol

journal_title

Journal of virology

authors

Hong K,Greer CE,Ketter N,Van Nest G,Paliard X

doi

10.1128/JVI.71.9.6427-6432.1997

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

6427-32

issue

9

eissn

0022-538X

issn

1098-5514

journal_volume

71

pub_type

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