Abstract:
:The gB glycoprotein of herpes simplex virus type 1 is involved in viral entry and fusion and contains a predicted membrane-anchoring sequence of 69 hydrophobic amino acids, which can span the membrane three times, near the carboxy terminus. To define the membrane-anchoring sequence and the role of this hydrophobic stretch, we have constructed deletion mutants of gB-1, lacking one, two, or three predicted membrane-spanning segments within the 69 amino acids. Expression of the wild-type and mutant glycoproteins in COS-1 cells show that mutant glycoproteins lacking segment 3 (amino acids 774 to 795 of the gB-1 protein) were secreted from the cells. Protease digestion and alkaline extraction of microsomes containing labeled mutant proteins further showed that segment 3 was sufficient for stable membrane anchoring of the glycoproteins, indicating that this segment may specify the transmembrane domain of the gB glycoprotein. Also, the mutant glycoproteins containing segment 3 were localized in the nuclear envelop, which is the site of virus budding. Deletion of any of the hydrophobic segments, however, affected the intracellular transport and processing of the mutant glycoproteins. The mutant glycoproteins, although localized in the nuclear envelope, failed to complement the gB-null virus (K082). These results suggest that the carboxy-terminal hydrophobic region contains essential structural determinants of the functional gB glycoprotein.
journal_name
J Viroljournal_title
Journal of virologyauthors
Rasile L,Ghosh K,Raviprakash K,Ghosh HPdoi
10.1128/JVI.67.8.4856-4866.1993subject
Has Abstractpub_date
1993-08-01 00:00:00pages
4856-66issue
8eissn
0022-538Xissn
1098-5514journal_volume
67pub_type
杂志文章abstract::The catalytic core domain (CCD) of human immunodeficiency virus type 1 (HIV-1) integrase (IN) harbors the enzyme active site and binds viral and chromosomal DNA during integration. Thirty-five CCD mutant viruses were constructed, paying particular attention to conserved residues in the Phe(139)-Gln(146) flexible loop ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2493-2505.2005
更新日期:2005-02-01 00:00:00
abstract::The major envelope glycoproteins gp120 and gp41 of human immunodeficiency virus type 1, the causative agent for human AIDS, contain numerous N-linked oligosaccharides. We report here our discovery that N-acetylglucosamine residues within the complex-type N-linked oligosaccharides of both gp120 and its precursor, gp160...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.943-952.1993
更新日期:1993-02-01 00:00:00
abstract::Human immunodeficiency virus type 1 contains a transmembrane glycoprotein with an unusually long cytoplasmic domain. To determine the role of this domain in virus replication, a series of single nucleotide changes that result in the insertion of premature termination codons throughout the cytoplasmic domain has been c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6616-6625.1992
更新日期:1992-11-01 00:00:00
abstract::A mutation (asparagine 815 to serine 815) was introduced into the herpes simplex virus type 1 (HSV-1) DNA polymerase (pol). The HSV-1 pol enzyme in lysates of Saccharomyces cerevisiae cells expressing the mutant protein showed increased resistance to acyclovir triphosphate and increased sensitivity to phosphonoacetate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4913-4918.1989
更新日期:1989-11-01 00:00:00
abstract::n-(n-Nonyl)-deoxygalactonojirimycin (n,n-DGJ), an alkylated imino sugar, reduces the amount of HBV DNA produced within the stably transfected HBV-producing HepG2.2.15 line in culture and is under consideration for development as a human therapeutic. n,n-DGJ does not appear to inhibit HBV DNA polymerase activity or env...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.11933-11940.2003
更新日期:2003-11-01 00:00:00
abstract::The Vpr protein, encoded by the human immunodeficiency virus type 1 (HIV-1) genome, is one of the nonstructural proteins packaged in large amounts into viral particles. We have previously reported that Vpr associates with the DNA repair enzyme uracil DNA glycosylase (UDG). In this study, we extended these observations...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1682-1688.1999
更新日期:1999-02-01 00:00:00
abstract::Transmission of human immunodeficiency virus type 1 (HIV-1) occurs primarily via the mucosal route, suggesting that HIV-1 vaccines may need to elicit mucosal immune responses. Here, we investigated the immunogenicity and relative efficacy of systemic immunization with two human ALVAC-HIV-1 recombinant vaccines express...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3732-3742.2006
更新日期:2006-04-01 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) infection is required for the development of several AIDS-related malignancies, including Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL). The high incidence of AIDS-KS has been ascribed to the interaction of KSHV and HIV-1. We have previously shown th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00797-16
更新日期:2016-09-12 00:00:00
abstract::Hepatitis C virus (HCV) is a single-stranded positive-sense RNA hepatotropic virus. Despite cellular defenses, HCV is able to replicate in hepatocytes and to establish a chronic infection that could lead to severe complications and hepatocellular carcinoma. An important player in subverting the host response to HCV in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00880-17
更新日期:2017-08-24 00:00:00
abstract::Primary HIV-1 infection (PHI) is marked by a flu-like syndrome and high levels of viremia that decrease to a viral set point with the first emergence of virus-specific CD8+ T-cell responses. Here, we investigated in a large cohort of 527 subjects the immunodominance pattern of the first virus-specific cytotoxic T-lymp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00182-09
更新日期:2009-08-01 00:00:00
abstract::Prior studies have demonstrated that a small proportion of blood lymphocytes from patients with human cytomegalovirus (HCMV) infection express only the viral immediate-early (IE) genes (L. Einhorn and A. Ost, J. Infect. Dis. 149:207-214, 1984; G. P. A. Rice, R. D. Schrier, and M. B. A. Oldstone, Proc. Natl. Acad. Sci....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3026-3033.1989
更新日期:1989-07-01 00:00:00
abstract::The Vpr accessory gene product of human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus is believed to play a role in permitting entry of the viral core into the nucleus of nondividing cells. A second role for Vpr was recently suggested by Rogel et al. (M. E. Rogel, L. I. Wu, and M. Emerman, J. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6705-6711.1995
更新日期:1995-11-01 00:00:00
abstract::The establishment of de novo-generated patterns of DNA methylation is characterized by the gradual spreading of DNA methylation (I. Kuhlmann and W. Doerfler, J. Virol. 47:631-636, 1983; M. Toth, U. Lichtenberg, and W. Doerfler, Proc. Natl. Acad. Sci. USA 86:3728-3732, 1989; M. Toth, U. Müller, and W. Doerfler J. Mol. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4301-4308.1991
更新日期:1991-08-01 00:00:00
abstract::The genome of infectious hematopoietic necrosis virus (IHNV), a salmonid novirhabdovirus, has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo By reverse genetics, eight recombinant IHNVs (rIHNVs), termed NxGy according to the respective po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01024-16
更新日期:2016-11-14 00:00:00
abstract::A number of cDNA clones complementary to measles virus mRNA and 50S genome RNA have been generated. These clones have been mapped by restriction enzyme analysis and were subsequently sequenced by the method of Maxam and Gilbert (A. M. Maxam and W. Gilbert, Methods Enzymol. 65:499-560, 1980). Computer analysis of these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.186-193.1985
更新日期:1985-04-01 00:00:00
abstract::RNA recombination occurs frequently during replication of tombusviruses and carmoviruses, which are related small plus-sense RNA viruses of plants. The most common recombinants generated by these viruses are either defective interfering (DI) RNAs or chimeric satellite RNAs, which are thought to be generated by templat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.12033-12047.2003
更新日期:2003-11-01 00:00:00
abstract::Encephalomyocarditis (EMC) virus (K-2 strain) was separated into two distinct peaks of optical density by chromatography on calcium phosphate, eluting at 0.17 and 0.31 m, respectively. The virus in these peaks could not be distinguished by infectivity (plaque-forming units), hemagglutinin activity, electron microscopi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.2.308-316.1967
更新日期:1967-04-01 00:00:00
abstract::We have previously demonstrated that two mouse cell lines that are poorly infectible by reovirus become highly susceptible upon transfection with the gene encoding the epidermal growth factor receptor (EGFR) (J. E. Strong, D. Tang, and P. W. K. Lee, Virology 197:405-411, 1993). This enhancement of infection efficiency...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.612-616.1996
更新日期:1996-01-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) capsid is a T=16 icosahedral shell that forms in the nuclei of infected cells. Capsid assembly also occurs in vitro in reaction mixtures created from insect cell extracts containing recombinant baculovirus-expressed HSV-1 capsid proteins. During capsid formation, the major capsi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3944-3951.1998
更新日期:1998-05-01 00:00:00
abstract::Rotavirus is the leading global cause of diarrheal mortality for unvaccinated children under 5 years of age. The outer capsid of rotavirus virions consists of VP7 and VP4 proteins, which determine viral G and P types, respectively, and are primary targets of neutralizing antibodies. Successful vaccination depends upon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01476-18
更新日期:2018-12-10 00:00:00
abstract::Retrovirus replication requires the production of both spliced and unspliced viral RNA from a single RNA transcript. In contrast, cellular RNA precursors with introns almost completely spliced. The cis elements and virus-coded trans factors which determine the extent to which Rous sarcoma virus RNA is spliced to src m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1669-1676.1989
更新日期:1989-04-01 00:00:00
abstract:UNLABELLED:Respiratory syncytial virus (RSV) is the most important cause of lower respiratory tract infection in young children and the leading cause of infant hospitalization worldwide. Uncontrolled response to RSV is mediated by a toll-like receptor (TLR)-mediated immune response. Resveratrol possesses anti-RSV activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03637-13
更新日期:2014-04-01 00:00:00
abstract::Open reading frame 45 (ORF45) of Kaposi's sarcoma-associated herpesvirus 8 (KSHV) is an immediate-early phosphorylated tegument protein and has been shown to play important roles at both early and late stages of viral infection. Homologues of ORF45 exist only in gammaherpesviruses, and their homology is limited. These...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02209-08
更新日期:2009-03-01 00:00:00
abstract::Candidate foot-and-mouth disease (FMD) DNA vaccines designed to produce viral capsids lacking infectious viral nucleic acid were evaluated. Plasmid DNAs containing a portion of the FMDV genome coding for the capsid precursor protein (P1-2A) and wild-type or mutant viral proteinase 3C (plasmids P12X3C or P12X3C-mut, re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4454-4457.1998
更新日期:1998-05-01 00:00:00
abstract::Lysyl-tRNA synthetase (LysRS) is packaged into human immunodeficiency virus type 1 (HIV-1) via its interaction with Gag, and this enzyme facilitates the selective packaging of tRNA(3)(Lys), the primer for initiating reverse transcription, into HIV-1. The Gag/LysRS interaction is detected at detergent-resistant membran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7553-7564.2004
更新日期:2004-07-01 00:00:00
abstract::CD81 has been described as a putative receptor for hepatitis C virus (HCV); however, its role in HCV cell entry has not been characterized due to the lack of an efficient cell culture system. We have examined the role of CD81 in HCV glycoprotein-dependent entry by using a recently developed retroviral pseudotyping sys...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1448-1455.2004
更新日期:2004-02-01 00:00:00
abstract::The tetrameric paramyxovirus hemagglutinin-neuraminidase (HN) protein mediates attachment to sialic acid-containing receptors as well as cleavage of the same moiety via its neuraminidase (NA) activity. The X-ray crystallographic structure of an HN dimer from Newcastle disease virus (NDV) suggests that a single site in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.12.6913-6922.2003
更新日期:2003-06-01 00:00:00
abstract::Adenovirus (Ad) replicative complexes form at discrete sites on the nuclear matrix (NM) via an interaction mediated by the precursor of the terminal protein (pTP). The identities of cellular proteins involved in these complexes have remained obscure. We present evidence that pTP binds to a multifunctional pyrimidine b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.2896-2904.1998
更新日期:1998-04-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is a ubiquitously distributed pathogen that causes severe disease in immunosuppressed patients and newborn infants infected in utero. The viral envelope glycoprotein B (gB) is an attractive molecule for active vaccination and passive immunoprophylaxis and therapy. Using human monoclonal an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00434-13
更新日期:2013-08-01 00:00:00
abstract::HIV-1 causes a persistent infection of the immune system that is associated with chronic comorbidities. The mechanisms that underlie this inflammation are poorly understood. Emerging literature has implicated proinflammatory purinergic receptors and downstream signaling mediators in HIV-1 infection. This study probed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01186-18
更新日期:2018-12-10 00:00:00