In vitro mutagenesis of the herpes simplex virus type 1 DNA polymerase gene results in altered drug sensitivity of the enzyme.

abstract：:Mice infected with myopathic coxsackievirus B1 Tucson (CVB1(T)) develop chronic inflammatory myopathy (CIM) consisting of hind limb weakness and inflammation. Amyopathic virus variants are infectious but attenuated for CIM. In this report, viral clones, chimeras, and sequencing were used to identify viral determinants...

journal_title：Journal of virology

authors： Tam PE,Weber-Sanders ML,Messner RP

更新日期：2003-11-01 00:00:00

abstract：:Hemagglutinin (HA) of H3N2/1968 pandemic influenza viruses differs from the putative avian precursor by seven amino acid substitutions. Substitutions Q226L and G228S are known to be essential for adaptation of avian HA to mammals. We found that introduction of avian-virus-like amino acids at five other HA positions (p...

journal_title：Journal of virology

authors： Van Poucke S,Doedt J,Baumann J,Qiu Y,Matrosovich T,Klenk HD,Van Reeth K,Matrosovich M

更新日期：2015-12-01 00:00:00

abstract：:High concentrations of either trypsin or chymotrypsin caused nearly complete cleavage of capsid protein VP2 of hepatitis A virus but did not significantly reduce the infectivity, thermostability, or antigenicity of the virus. Chymotrypsin also had a lesser effect on VP1. These findings indicate the presence of a prote...

journal_title：Journal of virology

authors： Lemon SM,Amphlett E,Sangar D

更新日期：1991-10-01 00:00:00

abstract：:The expression strategy of parvoviruses of the Densovirus genus has as yet not been reported. Clones were obtained from the densonucleosis virus of Galleria mellonella (GmDNV) that yielded infectious virus upon transfection into LD652 cells. Its genome was found to be the longest (6,039 nucleotides [nt]), with the lar...

journal_title：Journal of virology

authors： Tijssen P,Li Y,El-Far M,Szelei J,Letarte M,Zádori Z

更新日期：2003-10-01 00:00:00

abstract：:The enzymatic activity of recombinant influenza virus RNA polymerase is strictly dependent on the addition of a template RNA containing 5' and 3' viral sequences. Here we report the analysis of the binding specificity and physical characterization of the complex by using gel shift, modification interference, and densi...

journal_title：Journal of virology

authors： Tiley LS,Hagen M,Matthews JT,Krystal M

更新日期：1994-08-01 00:00:00

abstract：:Dendritic cells are central to the early events of human immunodeficiency virus type 1 (HIV-1) transmission, but HIV-1 infects dendritic cells inefficiently in vitro compared to activated CD4(+) T cells. There is a strong postentry restriction of HIV-1 infection in dendritic cells, partly mediated by the cellular rest...

journal_title：Journal of virology

authors： Pion M,Stalder R,Correa R,Mangeat B,Towers GJ,Piguet V

更新日期：2007-11-01 00:00:00

abstract：:A human cytomegalovirus (CMV) UL54 pol exonuclease domain II mutation, D413A, found in a clinical specimen, conferred ganciclovir (GCV) and cidofovir resistance but not foscarnet resistance when incorporated into laboratory strain T2294. After several passages without drug, mutation was observed in five of eight plaqu...

journal_title：Journal of virology

authors： Chou S,Marousek GI

更新日期：2008-01-01 00:00:00

abstract：:The agent(s) responsible for sporadic non-A, non-B hepatitis in humans was serially transmitted in rhesus monkeys by intravenous inoculation of the stool extract from a patient. A novel agent called HFV (hepatitis French [origin] virus) was present as 27- to 37-nm particles in the infectious stool extract. Hepatopathi...

journal_title：Journal of virology

authors： Deka N,Sharma MD,Mukerjee R

更新日期：1994-12-01 00:00:00

abstract：:A major challenge for the development of an effective HIV vaccine is to elicit neutralizing antibodies against a broad array of primary isolates. Monomeric gp120-based vaccine approaches have not been successful in inducing this type of response, prompting a number of approaches designed to recreate the native glycopr...

journal_title：Journal of virology

authors： Hammonds J,Chen X,Fouts T,DeVico A,Montefiori D,Spearman P

更新日期：2005-12-01 00:00:00

abstract：:Nonpermissive 3T3 cells were infected with purified superhelical simian virus 40 (SV40) deoxyribonucleic acid I (DNA I). One hour after infection, approximately 60% of the intracellular SV40 DNA was converted to relaxed forms. One day after infection, all intracellular SV40 DNA was present as slow-sedimenting material...

journal_title：Journal of virology

authors： Collins CJ,Sauer G

更新日期：1972-09-01 00:00:00

abstract：:Mucosal surfaces are the primary portals for human immunodeficiency virus (HIV) transmission. Because systemic immunization, in general, does not induce effective mucosal immune responses, a mucosal HIV vaccine is urgently needed. For this study, we developed papillomavirus pseudoviruses that express HIV-1 Gag. The ps...

journal_title：Journal of virology

authors： Zhang H,Fayad R,Wang X,Quinn D,Qiao L

更新日期：2004-10-01 00:00:00

abstract：:Some retroviruses with an extended repeat (R) region encode the polyadenylation signal within the R region such that this signal is present at both the 5' and 3' ends of the viral transcript. This necessitates differential regulation to either repress recognition of the 5' polyadenylation signal or enhance usage of th...

journal_title：Journal of virology

authors： Das AT,Klaver B,Berkhout B

更新日期：1999-01-01 00:00:00

abstract：:We used deep sequencing of poly(A) RNA to characterize the transcriptome of an economically important eel virus, anguillid herpesvirus 1 (AngHV1), at a stage during the lytic life cycle when infectious virus was being produced. In contrast to the transcription of mammalian herpesviruses, the overall level of antisense...

journal_title：Journal of virology

authors： van Beurden SJ,Gatherer D,Kerr K,Galbraith J,Herzyk P,Peeters BP,Rottier PJ,Engelsma MY,Davison AJ

更新日期：2012-09-01 00:00:00

abstract：:To determine the extent and structure of genetic variation in dengue viruses (DENV) on a restricted spatial and temporal scale, we sequenced the E (envelope) genes of DENV-1, -2, and -3 isolates collected in 2001 from children enrolled in a prospective school-based study in Kamphaeng Phet, Thailand, and diagnosed with...

journal_title：Journal of virology

authors： Jarman RG,Holmes EC,Rodpradit P,Klungthong C,Gibbons RV,Nisalak A,Rothman AL,Libraty DH,Ennis FA,Mammen MP Jr,Endy TP

更新日期：2008-06-01 00:00:00

abstract：:The required activities of CD4(+) T cells and antibody against the virally encoded oncoprotein simian virus 40 (SV40) Tag have previously been demonstrated by our laboratory to be mediators in achieving antitumor responses and tumor protection through antibody-dependent cell-mediated cytotoxicity (ADCC). In this study...

journal_title：Journal of virology

authors： Lowe DB,Shearer MH,Jumper CA,Bright RK,Kennedy RC

更新日期：2010-01-01 00:00:00

abstract：:During EBV infection, lytic DNA replication activates late gene expression in trans via an uncharacterized pathway. In this study, we mapped the target of this regulatory cascade to a variant TATA box (TATTAAA) and the 3' flanking region within the core promoter of the BcLF1 gene. The inherent late activity of this co...

journal_title：Journal of virology

authors： Serio TR,Cahill N,Prout ME,Miller G

更新日期：1998-10-01 00:00:00

abstract：:The Hsp70 chaperone plays a central role in multiple processes within cells, including protein translation, folding, intracellular trafficking, and degradation. This protein is implicated in the replication of numerous viruses. We have shown that rabies virus infection induced the cellular expression of Hsp70, which a...

journal_title：Journal of virology

authors： Lahaye X,Vidy A,Fouquet B,Blondel D

更新日期：2012-05-01 00:00:00

abstract：:The nature of the lesions involved in producing the fusiform phenotype of three mutants (WO101, WO201, and tsST529) of the Schmidt-Ruppin A strain of Rous sarcoma virus (RSV) was determined by molecular cloning and DNA sequencing. WO101 and WO201 contained an in-frame deletion of the v-src region coding for amino acid...

journal_title：Journal of virology

authors： Anderson SK,Fujita DJ

更新日期：1987-06-01 00:00:00

abstract：:Virion protein 16 (VP16) of herpes simplex virus type 1 (HSV-1) is a potent transcriptional activator of viral immediate-early (IE) genes. The VP16 activation domain can recruit various transcriptional coactivators to target gene promoters. However, the role of transcriptional coactivators in HSV-1 IE gene expression ...

journal_title：Journal of virology

authors： Kutluay SB,DeVos SL,Klomp JE,Triezenberg SJ

更新日期：2009-04-01 00:00:00

abstract：:We have investigated the mechanism of duck hepatitis B virus (DHBV) entry into susceptible primary duck hepatocytes (PDHs), using mutants of carboxypeptidase D (gp180), a transmembrane protein shown to act as the primary cellular receptor for avian hepatitis B virus uptake. The variant proteins were abundantly produce...

journal_title：Journal of virology

authors： Breiner KM,Schaller H

更新日期：2000-03-01 00:00:00

abstract：:The adenovirus (Ad) E4 ORF3 protein is both necessary and sufficient to reorganize a nuclear subdomain, the PML nuclear body (PML-NB), from punctate structures into elongated nuclear tracks. PML-NB disruption is recapitulated by a variety of DNA viruses that encode proteins responsible for compromising PML-NB integrit...

journal_title：Journal of virology

authors： Ullman AJ,Hearing P

更新日期：2008-08-01 00:00:00

abstract：:Simian immunodeficiency virus (SIV) infection of rhesus macaques has become an important surrogate model for evaluating HIV vaccine strategies. The extreme resistance to neutralizing antibody (NAb) of many commonly used strains, such as SIVmac251/239 and SIVsmE543-3, limits their potential relevance for evaluating the...

journal_title：Journal of virology

authors： Wu F,Ourmanov I,Kuwata T,Goeken R,Brown CR,Buckler-White A,Iyengar R,Plishka R,Aoki ST,Hirsch VM

更新日期：2012-08-01 00:00:00

abstract：:A map of bacteriophage lambda was constructed, including accurate positions for all 41 cut sites made by 12 different restriction enzymes. Over 100 fragments from single, multiple, and partial enzyme digestions were measured versus standards that were calibrated with respect to DNA molecules of known sequence. The dat...

journal_title：Journal of virology

authors： Daniels DL,de Wet JR,Blattner FR

更新日期：1980-01-01 00:00:00

abstract：:Leishmania RNA virus (LRV) is a double-stranded RNA virus that infects some strains of the protozoan parasite leishmania As with other totiviruses, LRV presumably expresses its polymerase by a ribosomal frameshift, resulting in a capsid-polymerase fusion protein. We have demonstrated previously that an LRV capsid-poly...

journal_title：Journal of virology

authors： Carrion R Jr,Ro YT,Patterson JL

更新日期：2003-10-01 00:00:00

abstract：:Coronaviruses generally have a narrow host range, infecting one or just a few species. Using targeted RNA recombination, we constructed a mutant of the coronavirus mouse hepatitis virus (MHV) in which the ectodomain of the spike glycoprotein (S) was replaced with the highly divergent ectodomain of the S protein of fel...

journal_title：Journal of virology

authors： Kuo L,Godeke GJ,Raamsman MJ,Masters PS,Rottier PJ

更新日期：2000-02-01 00:00:00

abstract：:Ultrastructure was correlated with growth kinetics of bdellophage VL-1 infecting host-dependent ("parasitic") Bdellovibrio bacteriovorus 109J in its Escherichia coli B host (the three-membered system), as well as in the host-independent ("saprophytic") derivative of the Bdellovibrio. Electron microscope observations s...

journal_title：Journal of virology

authors： Kessel M,Varon M

更新日期：1973-12-01 00:00:00

abstract：:Resistance to erythroleukemia induced by infection with the Friend virus complex (FV) has been mapped to several genes residing both within and outside the murine major histocompatibility complex (MHC). MHC genes located in the A, D, and Qa/Tla regions of the murine H-2 complex have been shown to affect disease resist...

journal_title：Journal of virology

authors： Perry LL,Miyazawa M,Hasenkrug K,Wehrly K,David CS,Chesebro B

更新日期：1994-08-01 00:00:00

abstract：:The synthesis of membrane protein after infection with bacteriophage T4 was examined. Protein constituents of both the cytoplasmic and outer membrane are made during the infective cycle. In addition, newly synthesized membrane protein is found in material which has a buoyant density greater than that of either of the ...

journal_title：Journal of virology

authors： Fletcher G,Wulff JL,Earhart CF

更新日期：1974-01-01 00:00:00

abstract：:The role of cellular genes in West Nile virus (WNV) replication is not well understood. Examination of cellular transcripts upregulated during WNV infection revealed an increase in the expression of the src family kinase (SFK) c-Yes. WNV-infected cell lines treated with the SFK inhibitor PP2 demonstrated a 2- to 4-log...

journal_title：Journal of virology

authors： Hirsch AJ,Medigeshi GR,Meyers HL,DeFilippis V,Früh K,Briese T,Lipkin WI,Nelson JA

更新日期：2005-09-01 00:00:00

abstract：:The retroviral Gag protein is capable of directing the production and release of virus-like particles in the absence of all other viral components. Budding normally occurs after Gag is transported to the plasma membrane by its membrane-targeting and -binding (M) domain. In the Rous sarcoma virus (RSV) Gag protein, the...

journal_title：Journal of virology

authors： Bowzard JB,Visalli RJ,Wilson CB,Loomis JS,Callahan EM,Courtney RJ,Wills JW

更新日期：2000-09-01 00:00:00