Abstract:
:During Epstein-Barr virus (EBV) latent infection of B lymphocytes in vitro, six EBV nuclear antigens (EBNAs) are expressed from one of two promoters, Cp and Wp, whose activities are mutually exclusive. Upon infection, Wp is initially active, followed by a switch to Cp for the duration of latency. In this study, the impact on Cp and Wp activity of sequences downstream of the distal EBNA gene promoter, Cp, was assessed in two lymphoblastoid cell lines. Cp activity was detected in constructs extending from just upstream of oriP to the first W1 exon. In contrast, Wp activity required the presence of the next downstream exon, W2. Viral sequences from -2199 to +2680 bp, relative to the Cp transcription start site, were dispensable for Wp activity. Sequences from +155 to +2680 bp, relative to the Cp transcription start site, were dispensable for Cp activity. Deletion of a 200-bp region from +2680 to +2880 bp downstream of Cp decreased both Cp and Wp activity two- to fivefold. Wp activity was also significantly diminished by deletion of the sequences from +2880 to +3000 bp downstream of the Cp transcription initiation site, which encompassed the Wp CCATT box. Based on deletion analyses of 10.3 kb of viral genomic sequence extending from just upstream of oriP to the first Wp, the only deletions which significantly upregulated Wp activity were those which abrogated Cp activity. However, reporter constructs in which the orientation of Cp was reversed displayed Wp activity comparable to that of reporter constructs in which Cp was deleted, even though the steady-state level of Cp-initiated transcripts from the inverted promoter was indistinguishable from that observed with Cp in normal orientation. This is the first direct evidence to support transcriptional interference as the mechanism for the mutually exclusive behavior of Cp and Wp.
journal_name
J Viroljournal_title
Journal of virologyauthors
Puglielli MT,Desai N,Speck SHdoi
10.1128/JVI.71.1.120-128.1997subject
Has Abstractpub_date
1997-01-01 00:00:00pages
120-8issue
1eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
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journal_title:Journal of virology
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doi:10.1128/JVI.15.5.1148-1157.1975
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doi:10.1128/JVI.71.8.6068-6074.1997
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pub_type: 杂志文章
doi:10.1128/JVI.70.7.4370-4379.1996
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journal_title:Journal of virology
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pub_type: 杂志文章
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abstract::In order to study cell tropism and attenuation of hepatitis A virus (HAV), the genome of HAV wild-type GBM and two cell culture-adapted variants, GBM/FRhK and GBM/HFS, were cloned and sequenced after amplification by reverse transcriptase-PCR. During virus cultivation, the HAV variant GBM/FRhK had a strict host range ...
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pub_type: 杂志文章
doi:10.1128/JVI.68.1.548-554.1994
更新日期:1994-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.2.305-315.1975
更新日期:1975-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.1.60-65.1984
更新日期:1984-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.2.688-692.1986
更新日期:1986-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01533-17
更新日期:2017-11-30 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3840-3852.1997
更新日期:1997-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02498-08
更新日期:2009-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.5117-5123.1995
更新日期:1995-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8798-8807.1997
更新日期:1997-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.275-278.1983
更新日期:1983-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.52.1.55-62.1984
更新日期:1984-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.320-329.1998
更新日期:1998-01-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.02911-15
更新日期:2016-04-14 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-09-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2287-2300.2005
更新日期:2005-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3146-3154.1998
更新日期:1998-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.926-932.1976
更新日期:1976-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7187-7195.1995
更新日期:1995-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.603-609.1982
更新日期:1982-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00348-07
更新日期:2007-08-01 00:00:00
abstract::Two hundred seventeen field isolates of cucumber mosaic cucumovirus (CMV), sampled from 11 natural populations, were typed by RNase protection assay (RPA) using probes from the genomic RNAs of strains in subgroup I and in subgroup II of CMV strains. Most (85%) of the analyzed isolates belonged to subgroup I. For these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.934-940.1997
更新日期:1997-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1141-1148.2003
更新日期:2003-01-01 00:00:00