Asymmetric transcription of mouse mammary tumor virus genes in vivo and in vitro.

abstract：:Nonconservative substitutions for Tyr-115 in the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) lead to enzymes displaying lower affinity for deoxynucleoside triphosphates (dNTPs) (A. M. Martín-Hernández, E. Domingo, and L. Menéndez-Arias, EMBO J. 15:4434-4442, 1996). Several mutations at th...

journal_title：Journal of virology

authors： Olivares I,Sánchez-Merino V,Martínez MA,Domingo E,López-Galíndez C,Menéndez-Arias L

更新日期：1999-08-01 00:00:00

abstract：:NS5A of the hepatitis C virus (HCV) is a highly phosphorylated protein involved in resistance against interferon and required most likely for replication of the viral genome. Phosphorylation of this protein is mediated by a cellular kinase(s) generating multiple proteins with different electrophoretic mobilities. In t...

journal_title：Journal of virology

authors： Koch JO,Bartenschlager R

更新日期：1999-09-01 00:00:00

abstract：:Duck hepatitis B virus mutants containing frameshift or stop codon mutations in a portion of the viral pol gene separating the terminal protein and reverse transcriptase domains had a leaky phenotype and, depending on the location and type of mutation, synthesized up to 10% as much viral DNA as did the wild type. This...

journal_title：Journal of virology

authors： Wu TT,Condreay LD,Coates L,Aldrich C,Mason W

更新日期：1991-05-01 00:00:00

abstract：:We isolated revertants of a polyomavirus whose origin of DNA replication contains a point mutation in the palindrome to which large T antigen binds. Four independent second-site revertants contain an Asp-286----Asn-286 substitution in large T antigen. This mutant large T antigen activates replication of DNAs containin...

journal_title：Journal of virology

authors： Tang WJ,Folk WR

更新日期：1989-01-01 00:00:00

abstract：:The copy choice model for the generation of defective interfering (DI) particles of vesicular stomatitis virus suggests that during replication the polymerase prematurely terminates, moves with the nascent daughter strand to another site on the same or a different template molecule, and resumes elongation of the nasce...

journal_title：Journal of virology

authors： Meier E,Harmison GG,Keene JD,Schubert M

更新日期：1984-08-01 00:00:00

abstract：:Human papillomavirus type 11 (HPV-11) and HPV-16 contain an E5 gene that can induce c-fos gene expression in mouse fibroblasts. This study investigated the human c-fos promoter characteristics by mapping the c-fos promoter sequence with several deletion and point mutants that confer responsiveness to E5 of HPV-11 or H...

journal_title：Journal of virology

authors： Chen SL,Lin YK,Li LY,Tsao YP,Lo HY,Wang WB,Tsai TC

更新日期：1996-12-01 00:00:00

abstract：:The role of glycosylation and of disulfide bonds in the formation of the native structure of the Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F0) glycoproteins was studied. In contrast to the HN and F0 synthesized in vivo, the proteins made from pSP6 transcripts in reticulocyte lysates, whether glycosylat...

journal_title：Journal of virology

authors： Vidal S,Mottet G,Kolakofsky D,Roux L

更新日期：1989-02-01 00:00:00

abstract：:Although the major component of the prion is believed to be the oligomer of PrP(Sc), little information is available concerning regions on the PrP(Sc) molecule that affect prion infectivity. During the analysis of PrP(Sc) molecules from various prion strains, we found that PrP(Sc) of the Chandler strain showed a uniqu...

journal_title：Journal of virology

authors： Shindoh R,Kim CL,Song CH,Hasebe R,Horiuchi M

更新日期：2009-04-01 00:00:00

abstract：:We reported previously that the movement protein (MP) of tomato mosaic tobamovirus is phosphorylated, and we proposed that MP phosphorylation is important for viral pathogenesis. Experimental data indicated that phosphorylation enhances the stability of MP in vivo and enables the protein to assume the correct intracel...

journal_title：Journal of virology

authors： Kawakami S,Hori K,Hosokawa D,Okada Y,Watanabe Y

更新日期：2003-01-01 00:00:00

abstract：:The feline immunodeficiency virus (FIV) cat model is extensively used to investigate possible vaccination approaches against AIDS in humans. Although consistent levels of protection have been achieved with FIV, as with other model systems, by immunizing with whole inactivated virus or fixed infected cells, the mechani...

journal_title：Journal of virology

authors： Mazzetti P,Giannecchini S,Del Mauro D,Matteucci D,Portincasa P,Merico A,Chezzi C,Bendinelli M

更新日期：1999-01-01 00:00:00

abstract：:The recent advent of genome sequences as the only source available to classify many newly discovered viruses challenges the development of virus taxonomy by expert virologists who traditionally rely on extensive virus characterization. In this proof-of-principle study, we address this issue by presenting a computation...

journal_title：Journal of virology

authors： Lauber C,Gorbalenya AE

更新日期：2012-04-01 00:00:00

abstract：:When human A431 cells, which carry high numbers of epidermal growth factor (EGF) receptors, are exposed to EGF, the total content of phosphotyrosine in cell protein is increased, the EGF receptor becomes phosphorylated at tyrosine, and new phosphotyrosine-containing 36,000- and 81,000-dalton proteins are detected. We ...

journal_title：Journal of virology

authors： Cooper JA,Scolnick EM,Ozanne B,Hunter T

更新日期：1983-12-01 00:00:00

abstract：UNLABELLED:Since the 1960s, simian hemorrhagic fever virus (SHFV; Nidovirales, Arteriviridae) has caused highly fatal outbreaks of viral hemorrhagic fever in captive Asian macaque colonies. However, the source(s) of these outbreaks and the natural reservoir(s) of this virus remain obscure. Here we report the identifica...

journal_title：Journal of virology

authors： Bailey AL,Lauck M,Sibley SD,Pecotte J,Rice K,Weny G,Tumukunde A,Hyeroba D,Greene J,Correll M,Gleicher M,Friedrich TC,Jahrling PB,Kuhn JH,Goldberg TL,Rogers J,O'Connor DH

更新日期：2014-11-01 00:00:00

abstract：:Two panels of monoclonal antibodies (MAbs) specific for the influenza A virus PA and PB2 polypeptides have been obtained from mice immunized with denatured proteins produced in Escherichia coli. All MAbs (13 specific for the PA polypeptide and 8 specific for the PB2 protein) reacted to the corresponding influenza viru...

journal_title：Journal of virology

authors： Bárcena J,Ochoa M,de la Luna S,Melero JA,Nieto A,Ortín J,Portela A

更新日期：1994-11-01 00:00:00

abstract：:Interspecies transmission of the transmissible spongiform encephalopathies (TSEs), or prion diseases, can result in the adaptation and selection of TSE strains with an expanded host range and increased virulence such as in the case of bovine spongiform encephalopathy and variant Creutzfeldt-Jakob disease. To investiga...

journal_title：Journal of virology

authors： Bartz JC,Bessen RA,McKenzie D,Marsh RF,Aiken JM

更新日期：2000-06-01 00:00:00

abstract：:The DNA sequence of 3,240 nucleotides of the XbaI G fragment located in the unique long (UL) region of the equine herpesvirus 1 genome revealed two major open reading frames (ORFs) designated UL3 and UL4. The UL3 ORF of 470 amino acids (aa) maps at nucleotides (nt) 4450 to 3038 from the long terminus, and its predicte...

journal_title：Journal of virology

authors： Zhao Y,Holden VR,Harty RN,O'Callaghan DJ

更新日期：1992-09-01 00:00:00

abstract：:Enviroxime is an antienterovirus compound that targets viral protein 3A and/or 3AB and suppresses a step in enterovirus replication by unknown mechanism. To date, four antienterovirus compounds, i.e., GW5074, Flt3 inhibitor II, TTP-8307, and AN-12-H5, are known to have similar mutations in the 3A protein-encoding regi...

journal_title：Journal of virology

authors： Arita M,Kojima H,Nagano T,Okabe T,Wakita T,Shimizu H

更新日期：2011-03-01 00:00:00

abstract：:The small envelope protein (E) plays a role of central importance in the assembly of coronaviruses. This was initially established by studies demonstrating that cellular expression of only E protein and the membrane protein (M) was necessary and sufficient for the generation and release of virus-like particles. To inv...

journal_title：Journal of virology

authors： Kuo L,Hurst KR,Masters PS

更新日期：2007-03-01 00:00:00

abstract：:The genome of murine cytomegalovirus, extracted from extracellular virions, is a linear double-stranded DNA molecule ca. 240 kilobase pairs long. In our initial cloning of subgenomic fragments of the murine cytomegalovirus genome, we obtained a HindIII clone which contained fused HindIII-terminal fragments. By hybridi...

journal_title：Journal of virology

authors： Marks JR,Spector DH

更新日期：1984-10-01 00:00:00

abstract：:The susceptibility of monocyte-derived cultured dendritic cells (DCs) to human immunodeficiency virus (HIV) infection and their role in viral transmission in the immune response were studied in detail. We observed that highly purified cultured DCs were infected with the T-tropic Lai strain of HIV type 1 (HIV-1Lai) via...

journal_title：Journal of virology

authors： Tsunetsugu-Yokota Y,Akagawa K,Kimoto H,Suzuki K,Iwasaki M,Yasuda S,Häusser G,Hultgren C,Meyerhans A,Takemori T

更新日期：1995-07-01 00:00:00

abstract：:We previously described a monoclonal antibody (Y8-10C2) that binds influenza virus hemagglutinin (HA) monomers but not native trimers. In this study, we demonstrated that Y8-10C2 binds to the globular domain of HA and found evidence that its epitope is located at the interface of adjacent subunits. We further showed t...

journal_title：Journal of virology

authors： Yewdell JW,Taylor A,Yellen A,Caton A,Gerhard W,Bächi T

更新日期：1993-02-01 00:00:00

abstract：:A member of the family Circoviridae, porcine circovirus type 2 (PCV2), is associated with postweaning multisystemic wasting syndrome (PMWS), a recent emerging disease worldwide. PCV2 is also found in clinically asymptomatic animals. This paradoxical finding makes the syndrome etiology challenging. We developed new ass...

journal_title：Journal of virology

authors： Khaiseb S,Sydler T,Zimmermann D,Pospischil A,Sidler X,Brugnera E

更新日期：2011-11-01 00:00:00

abstract：:mRNA's homologous to the herpes simplex virus type 1 DNA restriction endonuclease fragment BamHI p, which contains the thymidine kinase gene, have been identified and mapped by hybrid-arrested translation and mRNA selection. Such mRNA's, when translated in vitro, directed the synthesis of polypeptides of apparent mole...

journal_title：Journal of virology

authors： Preston CM,McGeoch DJ

更新日期：1981-05-01 00:00:00

abstract：:The large (L) protein of Ebola virus is a key protein for virus replication. Its N-terminal region harbors the RNA-dependent RNA polymerase activity, and its C terminus contains a cap assembling line composed of a capping domain and a methyltransferase domain (MTase) followed by a C-terminal domain (CTD) of unknown fu...

journal_title：Journal of virology

authors： Valle C,Martin B,Debart F,Vasseur JJ,Imbert I,Canard B,Coutard B,Decroly E

更新日期：2020-06-01 00:00:00

abstract：:Replicase gene expression by the human coronavirus 229E involves the synthesis of two large polyproteins, pp1a and pp1ab. Experimental evidence suggests that these precursor molecules are subject to extensive proteolytic processing. In this study, we show that a chymotrypsin-like enzyme, the virus-encoded 3C-like prot...

journal_title：Journal of virology

authors： Ziebuhr J,Siddell SG

更新日期：1999-01-01 00:00:00

abstract：:Adeno-associated virus serotype 9 (AAV9) vectors show promise for gene therapy of a variety of diseases due to their ability to transduce multiple tissues, including heart, skeletal muscle, and the alveolar epithelium of the lung. In addition, AAV9 is unique compared to other AAV serotypes in that it is capable of sur...

journal_title：Journal of virology

authors： Bell CL,Gurda BL,Van Vliet K,Agbandje-McKenna M,Wilson JM

更新日期：2012-07-01 00:00:00

abstract：:RNA silencing is a conserved eukaryotic gene regulatory system in which sequence specificity is determined by small RNAs. Plant RNA silencing also acts as an antiviral mechanism; therefore, viral infection requires expression of a silencing suppressor. The mechanism and the evolution of silencing suppression are still...

journal_title：Journal of virology

authors： Mérai Z,Kerényi Z,Molnár A,Barta E,Válóczi A,Bisztray G,Havelda Z,Burgyán J,Silhavy D

更新日期：2005-06-01 00:00:00

abstract：:Anaplastic nasopharyngeal carcinoma (NPC) cells invariably harbor the Epstein-Barr virus (EBV) genome, an association that is unique among human virus-associated cancers. Although EBV is able to replicate in epithelial cells, results with expression of the EBV receptor (complement receptor type 2 [CR2]; also called CD...

journal_title：Journal of virology

authors： Billaud M,Busson P,Huang D,Mueller-Lantzch N,Rousselet G,Pavlish O,Wakasugi H,Seigneurin JM,Tursz T,Lenoir GM

更新日期：1989-10-01 00:00:00

abstract：:The envelope protein E of flaviviruses mediates both receptor-binding and membrane fusion. At the virion surface, 180 copies of E are tightly packed and organized in a herringbone-like icosahedral structure, whereas in noninfectious subviral particles, 60 copies are arranged in a T=1 icosahedral symmetry. In both case...

journal_title：Journal of virology

authors： Kiermayr S,Stiasny K,Heinz FX

更新日期：2009-09-01 00:00:00

abstract：:The multifunctional simian and human immunodeficiency virus (SIV and HIV) Nef proteins are important for virulence. We studied the importance of selected Nef functions using an SIV Nef with mutations in two regions that are required for CD4 downregulation. This Nef mutant is defective for downregulating CD4 and, in ad...

journal_title：Journal of virology

authors： Iafrate AJ,Carl S,Bronson S,Stahl-Hennig C,Swigut T,Skowronski J,Kirchhoff F

更新日期：2000-11-01 00:00:00