Abstract:
:Porcine reproductive and respiratory syndrome (PRRS) consistently elevates the frequency of disease and mortality in young pigs. Many different secondary bacterial diseases occur in PRRS virus (PRRSV)-infected pigs. However, to date, establishing a reproducible experimental model of PRRSV infection in weaned pigs, with subsequent clinical disease following secondary bacterial challenge, has been difficult. PRRSV is frequently isolated during outbreaks from weak-born piglets affected by secondary bacterial diseases. This study was performed to investigate the potential role of intrauterine PRRSV infection on piglet susceptibility to secondary bacterial infection. PRRSV-free pregnant sows were intranasally infected at 98 days of gestation with PRRSV strain SD 23983. All piglets born to the PRRSV-infected sows were viremic. Piglets were removed from the sows at birth and deprived of colostrum. Piglets from PRRSV-infected and noninfected sows were randomly assigned to Streptococcus suis challenge or control subgroups. At 5 days of age, piglets were challenged intranasally with strain MN 87555 of S. suis type II. Total and differential leukocyte counts were performed on blood samples collected at 3 days of age. The numbers of leukocytes, lymphocytes, and monocytes were significantly reduced in the PRRSV-infected piglets. Lesions were observed in bone marrow, brain, lung, heart, spleen, lymph node, tonsil, and thymus of PRRSV-infected piglets. Thymus/body weight ratios of in utero PRRSV-infected piglets were significantly reduced compared to those of non-PRRSV-infected piglets, and thymic lesions were characterized by severe cortical depletion of thymocytes. Lesions were not observed in piglets born to PRRSV-free sows. Overall, 20 out of 22 piglets in the PRRSV-S. suis dual-infection group died within 1 week after challenge with S. suis (10 of 11 in each of two trials). This contrasts with 1 of 18 piglets in the PRRSV-infection-only group and 5 of 23 piglets in the S. suis-challenge-only group (1 of 12 in trial 1 and 4 of 11 in trial 2). No piglets died in the uninfected control groups. Most of the piglets in the PRRSV-S. suis dual-infection group developed suppurative meningitis. S. suis type II was recovered from their brains and joints. These results indicate that in utero infection by PRRSV makes piglets more susceptible to infection and disease following challenge by S. suis type II. In utero infection by PRRSV may provide a useful model to study the interaction between PRRSV and bacterial coinfections in piglets.
journal_name
J Viroljournal_title
Journal of virologyauthors
Feng W,Laster SM,Tompkins M,Brown T,Xu JS,Altier C,Gomez W,Benfield D,McCaw MBdoi
10.1128/JVI.75.10.4889-4895.2001subject
Has Abstractpub_date
2001-05-01 00:00:00pages
4889-95issue
10eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
杂志文章abstract::Molluscs, comprising one of the most successful phyla, lack clear evidence of adaptive immunity and yet thrive in the oceans, which are rich in viruses. There are thought to be nearly 120,000 species of Mollusca, most living in marine habitats. Despite the extraordinary abundance of viruses in oceans, molluscs often h...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00287-15
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abstract::Glycans on human immunodeficiency virus (HIV) envelope protein play an important role in infection and evasion from host immune responses. To examine the role of specific glycans, we introduced single or multiple mutations into potential N-linked glycosylation sites in hypervariable regions (V1 to V3) of the env gene ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01691-07
更新日期:2008-01-01 00:00:00
abstract::The product of the human cytomegalovirus (HCMV) UL71 gene is conserved throughout the herpesvirus family. During HCMV infection, protein pUL71 is required for efficient virion egress and is involved in the final steps of secondary envelopment leading to infectious viral particles. We found strong indications for oligo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06556-11
更新日期:2012-03-01 00:00:00
abstract::Senecavirus A (SVA) is a picornavirus that causes acute vesicular disease (VD), that is clinically indistinguishable from foot-and-mouth disease (FMD), in pigs. Notably, SVA RNA has been detected in lymphoid tissues of infected animals several weeks following resolution of the clinical disease, suggesting that the vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00819-19
更新日期:2019-10-15 00:00:00
abstract::The time course of murine leukemia virus production after chemical induction was determined in hamster-mouse somatic cell hybrids containing the xenotropic murine leukemia virus induction locus Bxv-1 or the ecotropic locus Akv-2. By using these hybrids, induction could be studied in the absence of secondary virus spre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.876-879.1984
更新日期:1984-09-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) encodes specific trafficking signals within its long cytoplasmic tail (CT) that regulate incorporation into HIV-1 particles. Rab11-family interacting protein 1C (FIP1C) and Rab14 are host trafficking factors required for Env particle incorpora...
journal_title:Journal of virology
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doi:10.1128/JVI.01893-17
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abstract::ICP0 is a transcriptional activating protein required for the efficient replication and reactivation of latent herpes simplex virus 1 (HSV-1). Multiple regions of ICP0 contribute its activity, the most prominent of which appears to be the RING finger, which confers E3 ubiquitin ligase activity. A region in the C termi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02265-10
更新日期:2011-05-01 00:00:00
abstract::During lytic infection, the genome of herpes simplex virus 1 (HSV-1) is associated with limited levels of histones but does not form a regular repeating nucleosomal structure. However, the previous observation that chromatin remodeling factors are recruited into viral replication compartments indicates that chromatin ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01575-08
更新日期:2008-12-01 00:00:00
abstract::Chronic HIV infection is characterized by chronic immune activation and dysfunctional T cells with elevated intracellular cyclic AMP (cAMP), which inhibits the T cell activation capability. cAMP may be induced by prostaglandin E(2) following lipopolysaccharide (LPS)-induced upregulation of cyclooxygenase type 2 (COX-2...
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pub_type: 杂志文章,随机对照试验
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更新日期:2011-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02114-10
更新日期:2011-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8148-8160.2002
更新日期:2002-08-01 00:00:00
abstract::Expression of the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax is correlated with cellular transformation contributing to the development of adult T-cell leukemia. Tax has been shown to modulate the activities of several cellular promoters. Existing evidence suggests that Tax need not directly bind to D...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.5.2161-2173.2001
更新日期:2001-03-01 00:00:00
abstract::Cottontail rabbit papillomavirus (CRPV)-induced papillomas progress at a high frequency to carcinomas and thus can serve as a model for high-cancer-risk human papillomavirus infection. Previously, we have shown that antibodies to nonstructural and structural proteins are detected in only a fraction of papilloma-bearin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.4043-4048.1994
更新日期:1994-06-01 00:00:00
abstract::The localization of the adenovirus type 5 34-kDa E4 and 55-kDa E1B proteins was determined in the absence of other adenovirus proteins. When expressed by transfection in human, monkey, hamster, rat, and mouse cell lines, the E1B protein was predominantly cytoplasmic and typically was excluded from the nucleus. When ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6323-6335.1996
更新日期:1996-09-01 00:00:00
abstract::We have previously designed in vitro model systems to characterize the herpes simplex virus type 1 (HSV-1) genome during in vitro virus latency. Latency was established by treatment of infected human embryo lung fibroblast (HEL-F) cells or rat fetal neurons with (E)-5-(2-bromovinyl)-2'-deoxyuridine and human leukocyte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.1.205-213.1984
更新日期:1984-01-01 00:00:00
abstract::The Kaposi's sarcoma-associated herpesvirus (KSHV) (or human herpesvirus 8) open reading frame (ORF) K15 encodes a putative integral transmembrane protein in the same genomic location as latent membrane protein 2A of Epstein-Barr virus. Ectopic expression of K15 in cell lines revealed the presence of several different...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.2.802-816.2002
更新日期:2002-01-01 00:00:00
abstract::Nucleocapsids were isolated from purified enveloped nucleocapsids of Plodia interpunctella granulosis virus by treatment with Nonidet P-40. When analyzed on sodium dodecyl sulfate-polyacrylamide gels, the nucleocapsids consisted of eight polypeptides. One of these, a major component with a molecular weight of 12,500 (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.866-876.1980
更新日期:1980-02-01 00:00:00
abstract::A putative transcription factor, C/EBP, isolated from rat liver nuclei, has been shown to bind to at least two different sequence motifs: the CCAAT promoter domain and a core sequence [GTGG(T/A)(T/A)(T/A)G] common to many viral enhancers, including simian virus 40 and human hepatitis B virus. It has been proposed that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.4.1517-1522.1990
更新日期:1990-04-01 00:00:00
abstract::Host cell tRNAs are recruited for use as primers to initiate reverse transcription in retroviruses. Human immunodeficiency virus type 1 (HIV-1) uses tRNA(Lys3) as the replication primer, whereas Rous sarcoma virus (RSV) uses tRNA(Trp). The nucleic acid (NA) chaperone function of the nucleocapsid (NC) domain of HIV-1 G...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
abstract::Identification of cellular receptors used by coronavirus (CoV) entry into the host cells is critical to an understanding of pathogenesis and to development of intervention strategies. The fourth CoV genus, Deltacoronavirus, evolutionarily related to the Gammacoronavirus, has just been defined recently. In the current ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00318-18
更新日期:2018-05-29 00:00:00
abstract::RNA-dependent RNA polymerases were found to be associated with two salmonid rhabdoviruses: infectious hematopoietic necrosis (IHN) virus and the virus of hemorrhagic septicemia (VHS). The protein composition of these rhabdoviruses closely resembles that of rabies virus rather than that of vesicular stomatitis virus (M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.839-843.1977
更新日期:1977-06-01 00:00:00
abstract::Human immunodeficiency virus type 1 uses ribosomal frameshifting for translation of the Gag-Pol polyprotein. Frameshift activities are thought to be tightly regulated. Analysis of gag p1 sequences from 270 plasma virions identified in 64% of the samples the occurrence of polymorphism that could lead to changes in ther...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7868-7873.2002
更新日期:2002-08-01 00:00:00
abstract::Ultrastructure was correlated with growth kinetics of bdellophage VL-1 infecting host-dependent ("parasitic") Bdellovibrio bacteriovorus 109J in its Escherichia coli B host (the three-membered system), as well as in the host-independent ("saprophytic") derivative of the Bdellovibrio. Electron microscope observations s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1522-1533.1973
更新日期:1973-12-01 00:00:00
abstract::Feline leukemia virus (FeLV) proviruses have been characterized from naturally occurring non-B-cell, non-T-cell tumors occurring in the spleens of infected cats. These proviruses exhibit a unique sequence motif in the long terminal repeat (LTR), namely, a 21-bp tandem triplication beginning 25 bp downstream of the enh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3324-3332.1995
更新日期:1995-06-01 00:00:00
abstract::The use of endocytic pathways by viral glycoproteins is thought to play various functions during viral infection. We previously showed in transfection assays that herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) is transported from the cell surface back to the trans-Golgi network (TGN) and that two motifs of gB...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2007-12-01 00:00:00
abstract::Interferon-induced proteins (IFPs) exert multiple functions corresponding to diverse interferon signals. However, the intracellular functions of many IFPs are not fully characterized. Here, we report that IFP35, a member of the IFP family with a molecular mass of 35 kDa, can interact with the bovine Tas (BTas) regulat...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
abstract::Turkeys inoculated with spleen extracts from lymphoproliferative disease (LPD)-affected birds developed viremia, followed by typical LPD lesions. Electron microscopy and biochemical characterization established that the virus present in the blood of infected turkeys is a type C retrovirus. The viral particles possess ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.351-357.1979
更新日期:1979-04-01 00:00:00
abstract::Herpes simplex virus (HSV) entry is dependent on the interaction of virion glycoprotein D (gD) with one of several cellular receptors. We previously showed that gD binds specifically to two structurally dissimilar receptors, HveA and HveC. We have continued our studies by using (i) a panel of baculovirus-produced gD m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.9879-9890.1999
更新日期:1999-12-01 00:00:00
abstract::The lymphocytic choriomeningitis virus (LCMV) genome consists of a large RNA segment and a small RNA segment. The three major structural proteins of this virus are an internal nucleoprotein and two surface glycoproteins. Intertypic reassortants between the Armstrong and WE strains of LCMV were made to map proteins enc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.3.966-968.1985
更新日期:1985-03-01 00:00:00
abstract::Interspecies transmission of the transmissible spongiform encephalopathies (TSEs), or prion diseases, can result in the adaptation and selection of TSE strains with an expanded host range and increased virulence such as in the case of bovine spongiform encephalopathy and variant Creutzfeldt-Jakob disease. To investiga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5542-5547.2000
更新日期:2000-06-01 00:00:00