Abstract:
:The activity of human immunodeficiency virus Rev as a regulator of viral mRNA expression is tightly linked to its ability to shuttle between the nucleus and cytoplasm; these properties are conferred by a leucine-rich nuclear export signal (NES) and by an arginine-rich nuclear localization signal/RNA binding domain (NLS/RBD) required for binding to the Rev-responsive element (RRE) located on viral unspliced and singly spliced mRNAs. Structure predictions and biophysical measurements indicate that Rev consists of an unstructured region followed by a helix-loop-helix motif containing the NLS/RBD and sequences directing multimerization and by a carboxy-terminal tail containing the NES. We present evidence that the loop portion of the helix-loop-helix region is an essential functional determinant that is required for binding to the RRE and for correct intracellular routing. Data obtained using a protein kinase CK2 phosphorylation assay indicated that the loop region is essential for juxtaposition of helices 1 and 2 and phosphorylation by protein kinase CK2. Deletion of the loop resulted in partial accumulation of Rev in SC35-positive nuclear bodies that resembled nuclear bodies that form in response to inhibition of transcription. Accumulation of the DeltaLoop mutant in nuclear bodies depended on the presence of an intact NES, suggesting that both the loop and the NES play a role in controlling intranuclear compartmentalization of Rev and its association with splicing factors.
journal_name
J Viroljournal_title
Journal of virologyauthors
D'Agostino DM,Ferro T,Zotti L,Meggio F,Pinna LA,Chieco-Bianchi L,Ciminale Vdoi
10.1128/jvi.74.24.11899-11910.2000subject
Has Abstractpub_date
2000-12-01 00:00:00pages
11899-910issue
24eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
杂志文章abstract::An unusual human retrovirus was isolated from two patients with persistent generalized lymphadenopathy who originate from West-Central Africa and are currently residing in Belgium. Although the virus shared a number of the same biological and morphological properties as human immunodeficiency retrovirus type 1 (HIV-1)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1207-1216.1990
更新日期:1990-03-01 00:00:00
abstract::Myxoma virus (MV) encodes a cell surface protein (M135R) that is predicted to mimic the host alpha/beta interferon receptor (IFN-alpha/beta-R) and thus prevent IFN-alpha/beta from triggering a host antiviral response. This prediction is based on sequence similarity to B18R, the viral IFN-alpha/beta-R from vaccinia vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01633-06
更新日期:2007-01-01 00:00:00
abstract::We have analyzed three cases of hepatitis C virus (HCV)-infected recipients who received blood from HCV-infected donors. Two recipients were exposed to two different HCV RNA-positive donors, and one was exposed to a single donor. All parental genomes from the actual infecting units of blood and the recipients were def...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.5.2059-2066.2001
更新日期:2001-03-01 00:00:00
abstract::Virus-specific proteins were examined in cultured cells infected with bovine viral diarrhea virus. By using antisera obtained from virus-infected animals, three major virus-specific polypeptides with molecular weights of 115,000 (115K), 80K, and 55K were observed. Minor proteins of 45,000 and 38,000 daltons were also ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.2.666-669.1984
更新日期:1984-05-01 00:00:00
abstract::The role of glycosylation and of disulfide bonds in the formation of the native structure of the Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F0) glycoproteins was studied. In contrast to the HN and F0 synthesized in vivo, the proteins made from pSP6 transcripts in reticulocyte lysates, whether glycosylat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.892-900.1989
更新日期:1989-02-01 00:00:00
abstract::In humans, homologous to the E6-AP carboxyl terminus (HECT) and regulator of chromosome condensation 1 (RCC1)-like domain-containing protein 5 (HERC5) is an interferon-induced protein that inhibits replication of evolutionarily diverse viruses, including human immunodeficiency virus type 1 (HIV-1). To better understan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00528-18
更新日期:2018-06-13 00:00:00
abstract::The innate and adaptive immune responses have evolved distinct strategies for controlling different viral pathogens. Encephalomyocarditis virus (EMCV) is a picornavirus that can cause paralysis, diabetes, and myocarditis within days of infection. The optimal innate immune response against EMCV in vivo requires CD1d. I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02745-05
更新日期:2006-07-01 00:00:00
abstract::The genome of the Neodiprion sertifer nucleopolyhedrovirus (NeseNPV), which infects the European pine sawfly, N. sertifer (Hymenoptera: Diprionidae), was sequenced and analyzed. The genome was 86,462 bp in size. The C+G content of 34% was lower than that of the majority of baculoviruses. A total of 90 methionine-initi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.7036-7051.2004
更新日期:2004-07-01 00:00:00
abstract::Studies of rotavirus morphogenesis, transport, and release have shown that although these viruses are released from the apical surface of polarized intestinal cells before cellular lysis, they do not follow the classic exocytic pathway. Furthermore, increasing evidence suggests that lipid rafts actively participate in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3935-3946.2006
更新日期:2006-04-01 00:00:00
abstract::Adult T-cell leukemia (ATL) is a fatal malignancy of CD4+ T cells infected with human T-cell leukemia virus type 1 (HTLV-1). ATL cells often exhibit random gross chromosomal rearrangements that are associated with the induction and improper repair of double-stranded DNA breaks (DSBs). The viral oncoprotein Tax has bee...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00672-18
更新日期:2018-07-17 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) genome contains three origins of DNA replication, one copy of oriL and two copies of oriS. Although oriL and oriS are structurally different, they have extensive nucleotide sequence similarity and can substitute for each other to initiate viral DNA replication. A fundamental que...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3580-3587.1997
更新日期:1997-05-01 00:00:00
abstract::Rhesus monkey rhadinovirus (RRV) is a gammaherpesvirus that is closely related to human Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8). RRV is the closest relative to KSHV that has a fully sequenced genome and serves as an in vitro and an in vivo model system for KSHV. The latency-associated nuclear antigen (LAN...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00704-09
更新日期:2009-10-01 00:00:00
abstract::Upon immunization and restimulation with tumors induced by the endogenous AKR/Gross murine leukemia virus (MuLV), C57BL/6 mice generate vigorous H-2K(b)-restricted cytotoxic T-lymphocyte (CTL) responses to a determinant (KSPWFTTL) derived from the p15E transmembrane portion of the viral envelope glycoprotein. By contr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3924-3928.2000
更新日期:2000-04-01 00:00:00
abstract::Control of HIV-1 replication following nonsterilizing HIV-1 vaccination could be achieved by vaccine-elicited CD8(+) T-cell-mediated antiviral activity. To date, neither the functional nor the phenotypic profiles of CD8(+) T cells capable of this activity are clearly understood; consequently, little is known regarding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00138-10
更新日期:2010-05-01 00:00:00
abstract::The ubiquitous human polyomavirus JC (JCV) is a small double-stranded DNA virus that establishes a persistent infection, and it is often transmitted from parents to children. There are at least 14 subtypes of the virus associated with different human populations. Because of its presumed codivergence with humans, JCV h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00441-06
更新日期:2006-10-01 00:00:00
abstract::Rotavirus is the major cause of diarrhea among young infants in both humans and animals. Immune protection of newborns by vaccination is difficult to achieve since there is not enough time to mount an immune response before exposure to the virus. We have designed a vaccination strategy mediating transfer of neutralizi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.8966-8971.2000
更新日期:2000-10-01 00:00:00
abstract::A region of the herpesvirus saimiri genome encoding an mRNA with two open reading frames (ORFs) has been identified to be essential for transformation of T cells. Deletion of either ORF resulted in the loss of transforming ability. ORF-1 has been shown to code for a collagen-like oncoprotein. This study shows for the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4495-4499.1995
更新日期:1995-07-01 00:00:00
abstract::Superinfection of Corynebacterium diphtheriae C7(beta) by heteroimmune phage gamma is productive, whereas superinfection by gamma-bin mutants is for the most part nonproductive. Exclusion of gamma-bin phage occurred after its DNA had penetrated and was partially expressed in the heteroimmune lysogen. All of the infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.526-532.1980
更新日期:1980-11-01 00:00:00
abstract::Nondefective reticuloendotheliosis virus induces chicken bursal lymphoma in a manner similar to that of avian leukosis virus. The provirus integrates in the c-myc locus and uses a promoter insertion mechanism to activate c-myc expression. We cloned a provirus involved in c-myc activation from a B lymphoma. Detailed st...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2084-2090.1987
更新日期:1987-07-01 00:00:00
abstract::We analyzed capsomeres of human papillomavirus type 16 (HPV16) consisting of the L1 major structural protein for their ability to trigger a cytotoxic T-cell (CTL) response. To this end, we immunized C57BL/6 mice and used the L1(165-173) peptide for ex vivo restimulation of splenocytes prior to analysis ((51)Cr release...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4635-4645.2003
更新日期:2003-04-01 00:00:00
abstract:UNLABELLED:The role of NF-κB in influenza A virus (IAV) infection does not reveal a coherent picture, as pro- and also antiviral functions of this transcription factor have been described. To address this issue, we used clustered regularly interspaced short palindromic repeat with Cas9 (CRISPR-Cas9)-mediated genome eng...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00946-16
更新日期:2016-08-12 00:00:00
abstract::Two serologically distinguishable primate herpesviruses, Herpesvirus aotus type 1 and type 3, were examined with regard to their genomes and structural polypeptides. The duplex DNA genomes of these two viruses were found to be essentially identical in molecular weight (Mr approximately equal to 145 X 10(6)) and guanin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.2.715-726.1983
更新日期:1983-02-01 00:00:00
abstract::We previously reported that human cytomegalovirus (HCMV) infection induces endoplasmic reticulum (ER) stress, resulting in activation of the unfolded protein response (UPR). Although some normal consequences of UPR activation (e.g., translation attenuation) are detrimental to viral infection, we have previously shown ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15388-15397.2005
更新日期:2005-12-01 00:00:00
abstract::Herpesvirus nucleocapsids traverse the nuclear envelope into the cytoplasm in a process called nuclear egress that includes disruption of the nuclear lamina. In several herpesviruses, a key player in nuclear egress is a complex of two proteins, whose homologs in human cytomegalovirus (HCMV) are UL50 and UL53. However,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02358-13
更新日期:2014-01-01 00:00:00
abstract::Propagation of the agents responsible for transmissible spongiform encephalopathies (TSEs) in cultured cells has been achieved for only a few cell lines. To establish efficient and versatile models for transmission, we developed neuroblastoma cell lines overexpressing type A mouse prion protein, MoPrP(C)-A, and then t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.1.320-325.2000
更新日期:2000-01-01 00:00:00
abstract::An extensive genetic map of coliphage P1 has been constructed for 113 amber mutants, using primarily a modification of the conventional complementation spot test. These spot tests failed to classify the mutants into cistrons, but when they were quantitated they permitted assignment of the mutants into 10 linkage clust...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.1.177-187.1976
更新日期:1976-10-01 00:00:00
abstract::Moloney murine leukemia virus (M-MuLV) IN-IN protein interactions important for catalysis of strand transfer and unimolecular and bimolecular disintegration reactions were investigated by using a panel of chemically modified M-MuLV IN proteins. Functional complementation of an HHCC-deleted protein (Ndelta105) by an in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1691-1698.1998
更新日期:1998-02-01 00:00:00
abstract::The EICP0 protein of equine herpesvirus 1 (EHV-1) is an early, viral regulatory protein that independently trans-activates EHV-1 immediate-early (IE), early, gamma1 late, and gamma2 late promoters. To assess whether this powerful trans-activator functions in conjunction with three other EHV-1 regulatory proteins to ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.3.1200-1208.2000
更新日期:2000-02-01 00:00:00
abstract::In this report, we describe some phenotypic properties of a temperature-sensitive mutant of herpes simplex type 1 (HSV-1) and present data concerning the physical location and nucleotide sequence of the genomic region harboring the mutation. The effect of shifts from the permissive to the nonpermissive temperature on ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2169-2179.1989
更新日期:1989-05-01 00:00:00
abstract::H9N2 influenza viruses are panzootic in domestic poultry in Eurasia and since 1999 have caused transient infections in humans and pigs. To investigate the zoonotic potential of H9N2 viruses, we studied the evolution of the viruses in live-poultry markets in Hong Kong in 2003. H9N2 was the most prevalent influenza viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8609-8614.2004
更新日期:2004-08-01 00:00:00