A subset of Pr65gag is nucleus associated in murine leukemia virus-infected cells.

Abstract:

:Nuclei of cells infected with Moloney murine leukemia virus (MoMuLV) were examined for the presence of gag proteins. This analysis was performed in conjunction with other studies suggesting a possible role for gag proteins in regulating nuclear events relating to processing and/or transport of viral genomic RNA. We detected Pr65gag and a p30-related protein in a nuclear fraction of infected cells. We also found evidence that a highly conserved amino acid sequence, which is shared by p30 and U1 small nuclear ribonucleoprotein 70-kDa protein, is a component of the nuclear targeting sequence for Pr65gag. Immunoelectron microscopy studies with a monoclonal anti-p12 antibody established that approximately 18% of gag-containing proteins of MoMuLV are located in the nucleus. Such gag-containing proteins from a mutant MoMuLV that lacks N-terminal myristic acid had greater affinity for the nucleus, suggesting that fatty acid acylation of Pr65gag plays a role in overcoming the proposed nuclear transport signal. The possible roles that nuclear gag proteins may play in retroviral replication are discussed.

journal_name

J Virol

journal_title

Journal of virology

authors

Nash MA,Meyer MK,Decker GL,Arlinghaus RB

doi

10.1128/JVI.67.3.1350-1356.1993

subject

Has Abstract

pub_date

1993-03-01 00:00:00

pages

1350-6

issue

3

eissn

0022-538X

issn

1098-5514

journal_volume

67

pub_type

杂志文章