Abstract:
:We analyzed a region of the capsid of canine parvovirus (CPV) which determines the ability of the virus to infect canine cells. This region is distinct from those previously shown to determine the canine host range differences between CPV and feline panleukopenia virus. It lies on a ridge of the threefold spike of the capsid and is comprised of five interacting loops from three capsid protein monomers. We analyzed 12 mutants of CPV which contained amino acid changes in two adjacent loops exposed on the surface of this region. Nine mutants infected and grew in feline cells but were restricted in replication in one or the other of two canine cell lines tested. Three other mutants whose genomes contain mutations which affect one probable interchain bond were nonviable and could not be propagated in either canine or feline cells, although the VP1 and VP2 proteins from those mutants produced empty capsids when expressed from a plasmid vector. Although wild-type and mutant capsids bound to canine and feline cells in similar amounts, infection or viral DNA replication was greatly reduced after inoculation of canine cells with most of the mutants. The viral genomes of two host range-restricted mutants and two nonviable mutants replicated to wild-type levels in both feline and canine cells upon transfection with plasmid clones. The capsids of wild-type CPV and two mutants were similar in susceptibility to heat inactivation, but one of those mutants and one other were more stable against urea denaturation. Most mutations in this structural region altered the ability of monoclonal antibodies to recognize epitopes within a major neutralizing antigenic site, and that site could be subdivided into a number of distinct epitopes. These results argue that a specific structure of this region is required for CPV to retain its canine host range.
journal_name
J Viroljournal_title
Journal of virologyauthors
Parker JS,Parrish CRdoi
10.1128/JVI.71.12.9214-9222.1997subject
Has Abstractpub_date
1997-12-01 00:00:00pages
9214-22issue
12eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::The structures of DNAs present in various intracellular forms of simian virus 40 (SV40) nucleoprotein complexes were analyzed by micrococcal nuclease digestion. The results showed that the 70S SV40 chromatin was completely sensitive to nuclease digestion, whereas CsCl gradient-purified mature virion was completely res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.603-609.1982
更新日期:1982-11-01 00:00:00
abstract::The Ebola virus envelope glycoprotein (GP) derived from the pathogenic Zaire subtype mediates cell rounding and detachment from the extracellular matrix in 293T cells. In this study we provide evidence that GPs from the other pathogenic subtypes, Sudan and Côte d'Ivoire, as well as from Reston, a strain thought to be ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.5.2518-2528.2002
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.10.4780-4791.2001
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abstract::The herpesvirus saimiri (HVS) immediate-early gene product encoded by open reading frame (ORF) 57 shares limited amino acid homology with HSV-1 ICP27 and Epstein-Barr virus BMLF1, both regulatory proteins. The ORF 57 gene has been proposed to be spliced based on the genome sequence, and here we confirm the intron-exon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.857-861.1998
更新日期:1998-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01104-20
更新日期:2020-08-17 00:00:00
abstract::Many of the approximately 165 proteins encoded by the African swine fever virus (ASFV) genome do not have significant similarity to known proteins and have not been studied experimentally. One such protein is DP148R. We showed that the DP148R gene is transcribed at early times postinfection. Deletion of this gene did ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01428-17
更新日期:2017-11-30 00:00:00
abstract::Human hepatitis B virus (HBV) HBx protein is a multifunctional protein that activates cellular signaling pathways and is thought to be essential for viral infection. Woodchuck HBV mutants that lack HBx are unable to replicate in vivo or are severely impaired. HBV replication in HepG2 cells, a human hepatoblastoma cell...
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pub_type: 杂志文章
doi:10.1128/jvi.77.14.7713-7719.2003
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abstract:UNLABELLED:Type I IFN signaling, which is initiated through activation of the alpha interferon receptor (IFNAR), regulates the expression of proteins that are crucial contributors to immune responses. Paramyxoviruses, including human metapneumovirus (HMPV), have evolved mechanisms to inhibit IFNAR signaling, but the sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03275-14
更新日期:2015-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2742-2746.1987
更新日期:1987-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02897-14
更新日期:2015-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4635-4645.2003
更新日期:2003-04-01 00:00:00
abstract::ts1 and ts7, two temperature-sensitive mutants of Moloney murine leukemia virus strain TB induce hind-limb paralysis in 100% of CFW/D mice injected. These two paralytogenic mutants also share a defect in their inability to process the env precursor protein, Pr80env, at the restrictive temperature. To identify the muta...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.2.364-373.1985
更新日期:1985-05-01 00:00:00
abstract::Hepatitis B virus (HBV) and woolly monkey hepatitis B virus (WMHBV) are primate hepadnaviruses that display restricted tissue and host tropisms. Hepatitis D virus (HDV) particles pseudotyped with HBV and WMHBV envelopes (HBV-HDV and WM-HDV) preferentially infect human and spider monkey hepatocytes, respectively, there...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.9786-9798.2005
更新日期:2005-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12572-12578.2003
更新日期:2003-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00201-14
更新日期:2014-06-01 00:00:00
abstract::The Ad2+ND4 virus is an adenovirus type 2 (Ad2)-simian virus 40 (SV40) recombination. The Ad2 genome of this recombinant has a rearrangement within early region 3; Ad2 DNA sequences between map positions 81.3 and 85.5 have been deleted, and the SV40 DNA sequences between map positions 0.11 and 0.626 have been inserted...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.2.344-353.1983
更新日期:1983-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2546-2551.1993
更新日期:1993-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.70.8.5564-5571.1996
更新日期:1996-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.3.935-942.1982
更新日期:1982-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2411-2414.1989
更新日期:1989-05-01 00:00:00
abstract::The alphaherpesvirus proteins UL31 and UL34 and their homologues in other herpesvirus subfamilies cooperate at the nuclear membrane in the export of nascent herpesvirus capsids. We studied the respective betaherpesvirus proteins M53 and M50 in mouse cytomegalovirus (MCMV). Recently, we established a random approach to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00681-10
更新日期:2010-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01178-06
更新日期:2006-11-01 00:00:00
abstract::The host cell receptor for Moloney murine leukemia virus was solubilized from murine L-cell membranes and characterized. In initial studies designed to identify a receptor-rich cell line, different mouse cells were screened for binding to Moloney gp70, the viral envelope glycoprotein which determines host cell-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.3.900-908.1986
更新日期:1986-06-01 00:00:00
abstract::Recent clinical trials examining 3'-azido-3'-deoxythymidine (AZT, zidovudine, or Retrovir) combined with L-2', 3'-dideoxy-3'-thiacytidine (3TC or lamivudine) have shown that combination therapy with these nucleoside analogs affords significant virological and clinical benefits. The addition of 3TC to AZT delays AZT re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.5093-5098.1998
更新日期:1998-06-01 00:00:00
abstract::The recent identification of coreceptors that mediate efficient entry of human immunodeficiency virus type 1 (HIV-1) suggests new therapeutic and preventive strategies. We analyzed simian immunodeficiency virus (SIV) entry cofactors to investigate whether the macaque SIV model can be used as an experimental model to e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6509-6516.1997
更新日期:1997-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00789-09
更新日期:2009-11-01 00:00:00
abstract::The group A rotaviruses are composed of at least seven serotypes. Serotype specificity is defined mainly by an outer capsid protein, VP7. In contrast, the other surface protein, VP3 (775 amino acids), appears to be associated with both serotype-specific and heterotypic immunity. To identify the cross-reactive and sero...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2421-2426.1988
更新日期:1988-07-01 00:00:00
abstract::As an approach to identifying hepatocyte receptors for the avian hepadnavirus duck hepatitis B virus (DHBV), hybridomas were prepared from mice immunized with permissive duck hepatocytes. Monoclonal antibodies (MAbs) were screened for the ability to inhibit binding of DHBV particles to primary duck hepatocytes and to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4829-4831.1997
更新日期:1997-06-01 00:00:00
abstract::This study identifies memory cytotoxic T lymphocyte (CTL) epitopes to respiratory syncytial virus (RSV) in healthy South African adults and demonstrates the conservation of those epitopes in circulating field strains of RSV in South Africa. Thirty-seven healthy adults from a population with diverse HLA backgrounds wer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7319-7329.2003
更新日期:2003-07-01 00:00:00