Abstract:
:10A1 murine leukemia virus can enter cells by using either of two different cell surface phosphate transport proteins, the gibbon ape leukemia virus receptor Glvr-1 (Pit-1) or the amphotropic retrovirus receptor Ram-1 (Pit-2). Glvr-1 and Ram-1 are widely expressed in different tissues, but the relative amounts of each are highly variable. We have developed retrovirus packaging cell lines based on 10A1 virus to take advantage of this dual receptor utilization to improve gene transfer rates in somatic cells of animals and humans, in which the relative levels of the two receptors are not always known. Optimization of the Env expression vector allowed the generation of packaging lines that produce helper-free vector titers up to 10(7)/ml. By interference analysis, we found that a 10A1 pseudotype retroviral vector can utilize Ram-1 for efficient entry into mouse, rat, and human cells and can utilize Glvr-1 for entry into mouse and human cells but not for entry into rat cells. The 10A1 pseudotype vector efficiently enters mouse cells by using Glvr-1, while entry into human cells is much less efficient. Thus, the 10A1 pseudotype packaging cells may be advantageous compared with the standard amphotropic packaging cells because vectors produced by the cells can use an additional receptor for cell entry. These packaging cells will also be useful to further explore the complicated pattern of receptor usage conferred by the 10A1 viral surface protein.
journal_name
J Viroljournal_title
Journal of virologyauthors
Miller AD,Chen Fdoi
10.1128/JVI.70.8.5564-5571.1996subject
Has Abstractpub_date
1996-08-01 00:00:00pages
5564-71issue
8eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3451-3454.1998
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.766-775.1994
更新日期:1994-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.653-661.1980
更新日期:1980-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.857-864.1984
更新日期:1984-03-01 00:00:00
abstract:UNLABELLED:Molluscum contagiosum virus (MOCV), the only circulating human-specific poxvirus, has a worldwide distribution and causes benign skin lesions that may persist for months in young children and severe infections in immunosuppressed adults. Studies of MOCV are restricted by the lack of an efficient animal model...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02911-15
更新日期:2016-04-14 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.00730-12
更新日期:2012-07-01 00:00:00
abstract::Since dendritic cells may play a key role in defense against influenza virus infection, we examined the effects of recombinant hemagglutinin (HA) proteins derived from mouse-adapted H1N1 (A/WSN/1933), swine-origin 2009 pandemic H1N1 (A/Texas/05/2009), and highly pathogenic avian influenza H5N1 (A/Thailand/KAN-1/2004) ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01316-10
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pub_type: 杂志文章
doi:10.1128/JVI.65.6.3192-3202.1991
更新日期:1991-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.79.8.5047-5058.2005
更新日期:2005-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.2861-2868.1996
更新日期:1996-05-01 00:00:00
abstract::In gene therapy, the innate immune system is a significant barrier to the effective application of adenovirus (Ad) vectors. In kidney epithelium-derived (REC) cells, serotype 5 Ad vectors induce the expression of the chemokine CXCL10 (IP-10), a response that is dependent on NFkappaB. Compared to the parental vector Ad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14507-14515.2005
更新日期:2005-12-01 00:00:00
abstract::Unintegrated linear and closed circular DNAs of B- and N-tropic endogenous BALB/c murine leukemia virus (MuLV) were extracted from newly infected mouse cells and cleaved with EcoRI, XhoI, PvuI, HindIII, SalI, XbaI, KpnI, SmaI, and PstI restriction endonucleases. The DNA fragments were separated by electrophoresis and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.812-823.1980
更新日期:1980-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8181-8190.1998
更新日期:1998-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9934-9951.2002
更新日期:2002-10-01 00:00:00
abstract::The influenza A virus nucleoprotein (NP) is a single-stranded RNA-binding protein that encapsidates the virus genome and has essential functions in viral-RNA synthesis. Here, we report the characterization of a temperature-sensitive (ts) NP mutant (US3) originally generated in fowl plague virus (A/chicken/Rostock/34)....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01424-08
更新日期:2009-01-01 00:00:00
abstract::Friend murine spleen focus-forming virus (SFFV) encodes a glycoprotein designated gp52, which is responsible for the leukemogenic properties of the virus. gp52 lacks a cytoplasmic domain and is defective in its transport to the cell surface. We constructed a chimeric envelope gene which codes for a molecule with an ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4007-4011.1987
更新日期:1987-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03186-12
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pub_type: 杂志文章
doi:10.1128/JVI.34.1.280-284.1980
更新日期:1980-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.3.584-589.1979
更新日期:1979-09-01 00:00:00
abstract::The Vpr protein, encoded by the human immunodeficiency virus type 1 (HIV-1) genome, is one of the nonstructural proteins packaged in large amounts into viral particles. We have previously reported that Vpr associates with the DNA repair enzyme uracil DNA glycosylase (UDG). In this study, we extended these observations...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1682-1688.1999
更新日期:1999-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1707-1711.1993
更新日期:1993-03-01 00:00:00
abstract::In infected cells, hepatitis C virus (HCV) induces the formation of membrane alterations referred to as membranous webs, which are sites of RNA replication. In addition, HCV RNA replication also occurs in smaller membrane structures that are associated with the endoplasmic reticulum. However, cellular mechanisms invol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01190-09
更新日期:2010-01-01 00:00:00
abstract::The small envelope protein (E) plays a role of central importance in the assembly of coronaviruses. This was initially established by studies demonstrating that cellular expression of only E protein and the membrane protein (M) was necessary and sufficient for the generation and release of virus-like particles. To inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01577-06
更新日期:2007-03-01 00:00:00
abstract::Nucleotide sequencing and phylogenetic analysis of 10 recognized prototype strains of the porcine enterovirus (PEV) cytopathic effect (CPE) group I reveals a close relationship of the viral genomes to the previously sequenced strain F65, supporting the concept of a reclassification of this virus group into a new picor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.4.1620-1631.2001
更新日期:2001-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.2.785-793.2006
更新日期:2006-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3240-3249.2001
更新日期:2001-04-01 00:00:00
abstract:UNLABELLED:Retargeting of gammaretroviral envelope proteins has shown promising results in the isolation of novel isolates with therapeutic potential. However, the optimal conditions required to obtain high-affinity retargeted envelope proteins with narrow tropism are not understood. This study highlights the advantage...
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pub_type: 杂志文章
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更新日期:2015-12-30 00:00:00
abstract::Worldwide amphibian population declines have been ascribed to global warming, increasing pollution levels, and other factors directly related to human activities. These factors may additionally be favoring the emergence of novel pathogens. In this report, we have determined the complete genome sequence of the emerging...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.07108-11
更新日期:2012-04-01 00:00:00