Abstract:
:Previous work on the strict late (gamma) UL38 promoter of herpes simplex virus type 1 identified three cis-acting elements required for wild-type levels of transcription: a TATA box at -31, a consensus mammalian initiator element at the transcription start site, and a downstream activation sequence (DAS) at +20 to +33. DAS is found in similar locations on several other late promoters, suggesting an important regulatory role in late gene expression. In this communication, we further characterize the interaction between DAS and a cellular protein which is found in both uninfected and infected nuclear extracts. This protein was purified from HeLa nuclear extracts and identified as the DNA binding component (Ku heterodimer) of DNA-dependent protein kinase (DNA-PK) by peptide mapping. Highly purified DNA-PK was able to stimulate UL38 transcription in vitro approximately 10-fold. DAS is similar in sequence to another element, nuclear regulatory element 1 (NRE1) of the glucocorticoid-responsive mouse mammary tumor virus long terminal repeat. NRE1 is known to specifically bind Ku in the absence of DNA ends. We demonstrated that NRE1 is able to substitute for DAS in the UL38 promoter to activate transcription as measured by in vitro transcription and in vivo during infection of tissue culture cells with recombinant virus. Also, we found that the binding of DNA-PK to DAS involves the bases demonstrated to be important in UL38 transcription and that the 70-kDa subunit of Ku binds to DAS.
journal_name
J Viroljournal_title
Journal of virologyauthors
Petroski MD,Wagner EKdoi
10.1128/JVI.72.10.8181-8190.1998subject
Has Abstractpub_date
1998-10-01 00:00:00pages
8181-90issue
10eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::Grasshoppers, Melanoplus sanguinipes (F.), infected with the grasshopper inclusion body virus (GIBV) showed a general torpor, took longer to develop, and had abnormally high rates of mortality. Infection was found only in the fat body, and developing viruses and inclusion bodies were observed in the nuclei and cytopla...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.6.605-610.1969
更新日期:1969-06-01 00:00:00
abstract::An immediate-early protein of murine cytomegalovirus (MCMV), pp89, elicits an immunodominant and protective major histocompatibility complex (MHC) class I Ld-restricted CD8+ T-lymphocyte response. Remarkably, presentation of the naturally processed peptide of pp89, the nonapeptide YPHFMPTNL, is abolished during permis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.289-297.1994
更新日期:1994-01-01 00:00:00
abstract::A fresh inoculum of human adenovirus type 12 (Ad12) was obtained from the American Type Culture Collection and passaged once on human embryonic kidney cells, and Ad12 DNA was prepared from the first-passage yield to avoid higher passages which might have generated host-virus DNA recombinants. The 18 PstI fragments of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.379-389.1994
更新日期:1994-01-01 00:00:00
abstract::We have developed a system for producing murine leukemia virus (MLV) pseudotyped with human hepatitis B virus (HBV) large (L) and small (S) surface antigens (HBsAg) for targeting primary human hepatocytes. Using the MLV(HBV) pseudotype virus containing a beta-galactosidase reporter gene, we demonstrated that this pseu...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.76.2.912-917.2002
更新日期:2002-01-01 00:00:00
abstract::Vpu is a 16-kDa membrane-associated phosphoprotein that is expressed from the same, singly spliced message as the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein precursor, gp160. Previous studies suggest that Vpu functions in the late stages of viral replication, possibly in virus egression from the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4190-4194.1993
更新日期:1993-07-01 00:00:00
abstract::Eucaryotic, viral, and bacteriophage DNA polymerases of the alpha-like family share blocks of sequence similarity, the most conserved of which has been designated region I. Region I includes a YGDTDS motif that is almost invariant within the alpha-like family and that is similar to a motif conserved among RNA-directed...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5883-5890.1990
更新日期:1990-12-01 00:00:00
abstract::Since 2005, a large poliomyelitis outbreak associated with type 2 circulating vaccine-derived poliovirus (cVDPV2) has occurred in northern Nigeria, where immunization coverage with trivalent oral poliovirus vaccine (tOPV) has been low. Phylogenetic analysis of P1/capsid region sequences of isolates from each of the 40...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02954-12
更新日期:2013-05-01 00:00:00
abstract::Adenoviral RNA sequences synthesized in nuclei isolated during the late phase of productive infection comprise, besides virus-associated, VA, RNA, five major species, ranging in size from approximately 13S to 55S. The latter RNA species is of the length predicted if the major transcriptional unit expressed during the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.2.394-403.1979
更新日期:1979-11-01 00:00:00
abstract::The HTLV-1 singly spliced open reading frame I protein, p12(I), is highly unstable and appears to be necessary for persistent infection in rabbits. Here we demonstrate that p12(I) forms dimers through two putative leucine zipper domains and that its stability is augmented by specific proteasome inhibitors. p12(I) is u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6460-6467.1999
更新日期:1999-08-01 00:00:00
abstract::The minor glycoproteins from hepatitis B surface antigen, GP33 and GP36, contain at their carboxy-terminal part the sequence of the major protein P24. They have 55 additional amino acids at the amino-terminal part which are coded by the pre-S region of the viral DNA. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.626-628.1983
更新日期:1983-05-01 00:00:00
abstract::Human papillomavirus type 11 (HPV-11) and HPV-16 contain an E5 gene that can induce c-fos gene expression in mouse fibroblasts. This study investigated the human c-fos promoter characteristics by mapping the c-fos promoter sequence with several deletion and point mutants that confer responsiveness to E5 of HPV-11 or H...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8558-8563.1996
更新日期:1996-12-01 00:00:00
abstract::The effect of NS1 protein on the efficiency of influenza virus mRNA translation was evaluated by determining the accumulation of nucleoprotein (NP) or M1 mRNAs in the cytoplasm of cells expressing either of these genes alone or in combination with the NS1 gene, as well as the total cell accumulation of NP or M1 protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2427-2433.1995
更新日期:1995-04-01 00:00:00
abstract::Hepatitis C virus (HCV) replication in infected patients produces large and diverse viral populations, which give rise to drug-resistant and immune escape variants. Here, we analyzed HCV populations during transmission and diversification in longitudinal and cross-sectional samples using 454/Roche pyrosequencing, in t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02271-09
更新日期:2010-06-01 00:00:00
abstract::We have determined the complete nucleotide sequence of an infectious cloned genome of ground squirrel hepatitis virus (GSHV), a nonpathogenic member of the hepadnavirus group. The genome is 3,311 base pairs long and contains the major open reading frames described for the related human and woodchuck hepatitis B viruse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.2.367-375.1984
更新日期:1984-08-01 00:00:00
abstract::Experimental evidence from the simian immunodeficiency virus (SIV) model of AIDS has shown that the nef gene is critical in the pathogenesis of AIDS. Consequently, nef is of considerable interest in both antiviral drug and vaccine development. Preliminary findings in two rhesus macaques indicated that a deletion of on...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.5117-5123.1995
更新日期:1995-08-01 00:00:00
abstract::Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the complex cis-acting oriLyt region, which spans nearly 3 kb. DNA synthesis requires six core proteins together with UL84 and IE2. Previously, two essential regions were identified within oriLyt. Essential region I (nucleotides [nt] 92209 to 92573) ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00058-07
更新日期:2007-07-01 00:00:00
abstract::A prerequisite for understanding the molecular function of the human cytomegalovirus (HCMV) gH (UL75)-gL (UL115) complex is a detailed knowledge of the structure of this complex in its functional form, as it is present in mature virions. The gH protein is known to be a component of a 240-kDa envelope complex designate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5391-5398.1997
更新日期:1997-07-01 00:00:00
abstract::An infectious cDNA clone of a genotype 3 strain of hepatitis E virus adapted to growth in HepG2/C3A human hepatoma cells was constructed. This virus was unusual in that the hypervariable region of the adapted virus contained a 171-nucleotide insertion that encoded 58 amino acids of human S17 ribosomal protein. Analyse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00146-12
更新日期:2012-05-01 00:00:00
abstract::African swine fever virus induces in convalescent pigs antibodies that neutralized the virus before and after binding to susceptible cells, inhibiting both virus attachment and internalization. A further analysis of the neutralization mechanisms mediated by the different viral proteins showed that antibodies to protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5689-5694.1996
更新日期:1996-08-01 00:00:00
abstract::Five gibbon ape leukemia virus substrains (two from gibbons with lymphocytic leukemia and three from gibbons with myelogenous leukemia) were examined for unique genomic sequences specific for each form of leukemia. By using sequential adsorption procedures, the genome from each gibbon ape leukemia virus was fractionat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.400-408.1980
更新日期:1980-08-01 00:00:00
abstract::Humans differ substantially with respect to susceptibility to human immunodeficiency virus type 1 (HIV-1). We evaluated variants of nine host genes participating in the viral life cycle for their role in modulating HIV-1 infection. Alleles were assessed ex vivo for their impact on viral replication in purified CD4 T c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12674-12680.2005
更新日期:2005-10-01 00:00:00
abstract::In vitro replication of coxsackievirus B3 (CVB3) in cells of the immune system derived from uninfected adolescent A/J and C57BL/6J mice and replication of CVB3 in and association with immune cells from spleens of infected animals in vivo were assessed. Nonstimulated or mitogen-stimulated spleen cells were minimally pe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4632-4645.1996
更新日期:1996-07-01 00:00:00
abstract::The cellular site of herpesvirus tegument assembly has yet to be defined. We have previously used a recombinant herpes simplex virus type 1 expressing a green fluorescent protein (GFP)-tagged tegument protein, namely VP22, to show that VP22 is localized exclusively to the cytoplasm during infection. Here we have const...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.6.2575-2583.2001
更新日期:2001-03-01 00:00:00
abstract::Tumor progression locus 2 (TPL2) is a serine/threonine kinase that belongs to the mitogen-activated protein 3 kinase (MAP3K) family, and it plays an important role in pathogen infection. The trimer complex of TPL2, p105, and ABIN2 is essential for maintenance of TPL2 steady-state levels and host cell response to patho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02149-20
更新日期:2020-12-23 00:00:00
abstract::n-(n-Nonyl)-deoxygalactonojirimycin (n,n-DGJ), an alkylated imino sugar, reduces the amount of HBV DNA produced within the stably transfected HBV-producing HepG2.2.15 line in culture and is under consideration for development as a human therapeutic. n,n-DGJ does not appear to inhibit HBV DNA polymerase activity or env...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.11933-11940.2003
更新日期:2003-11-01 00:00:00
abstract::The ability of human immunodeficiency virus type 1 (HIV-1) to transduce nondividing cells is key to infecting terminally differentiated macrophages, which can serve as a long-term reservoir of HIV-1 infection. The mutation N57A in the viral CA protein renders HIV-1 cell cycle dependent, allowing examination of HIV-1 i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00214-19
更新日期:2019-04-17 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is the leading cause of serious pediatric acute respiratory tract infections, and a better understanding is needed of the host response to HRSV and its attenuated vaccine derivatives. It has been shown previously that HRSV nonstructural proteins 1 and 2 (NS1 and NS2) inhibit th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5353-5362.2005
更新日期:2005-05-01 00:00:00
abstract::In cell-free systems, 2-5A [ppp(A2'p)nA, n = 2 to greater than or equal to 4] activates a latent endoribonuclease, the 2-5A-dependent RNase, which cleaves rRNA in intact ribosomes into discrete and characteristic products (D. H. Wreschner et al., Nucleic Acids Res. 9:1571-1581, 1981). Here we present Northern blots wh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.3.1051-1055.1983
更新日期:1983-06-01 00:00:00
abstract::Formation of a six-helix bundle comprised of three C-terminal heptad repeat regions in antiparallel orientation in the grooves of an N-terminal coiled-coil is critical for promotion of membrane fusion by paramyxovirus fusion (F) proteins. We have examined the effect of mutations in four residues of the N-terminal hept...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1543-1551.2005
更新日期:2005-02-01 00:00:00
abstract::A distinctive feature of chronic human immunodeficiency virus type 1 (HIV-1) infection is the presence of multiple coexisting genetic variants, or subpopulations, that comprise the HIV-1 population detected in the peripheral blood. Analysis of HIV-1 RNA decay dynamics during the initiation of highly active antiretrovi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02486-08
更新日期:2009-05-01 00:00:00