Dendritic cell activation by recombinant hemagglutinin proteins of H1N1 and H5N1 influenza A viruses.

abstract：:The structure and replication of a cold-adapted, temperature-sensitive (TS) mutant of an Asian (H2N2) influenza virus was compared with that of its wild-type (WT) parent. Viruses were grown in a chicken kidney cell system, and at the nonpermissive temperature of 40 C, production of infectious TS virus was about 100,00...

journal_title：Journal of virology

authors： Kendal AP,Kiley MP,Maassab HF

更新日期：1973-12-01 00:00:00

abstract：:Recently, significant numbers of individuals with primary human immunodeficiency virus (HIV) infection have been found to harbor viral strains with reduced susceptibility to antiretroviral drugs. In one study, HIV from 16% of such antiretroviral-naive individuals was shown to have a susceptibility to nonnucleoside rev...

journal_title：Journal of virology

authors： Brown AJ,Precious HM,Whitcomb JM,Wong JK,Quigg M,Huang W,Daar ES,D'Aquila RT,Keiser PH,Connick E,Hellmann NS,Petropoulos CJ,Richman DD,Little SJ

更新日期：2000-11-01 00:00:00

abstract：:Reverse transcriptases (RTs) are found in a wide variety of mobile genetic elements including viruses, retrotransposons, and infectious organellar introns. An invariant triad of aspartates is thought to be required for the catalytic function of RTs. We generated RT mutants in the yeast retrotransposon Ty1, changing ea...

journal_title：Journal of virology

authors： Uzun O,Gabriel A

更新日期：2001-07-01 00:00:00

abstract：:Sequencing and reversion analysis of murine hepatitis virus (MHV) temperature-sensitive (ts) viruses has identified putative ts mutations in the replicase nonstructural proteins (nsp's) of these coronaviruses. In this study, reverse transcriptase PCR sequencing of the RNA genome of an isolate of the MHV ts virus Alb t...

journal_title：Journal of virology

authors： Sparks JS,Donaldson EF,Lu X,Baric RS,Denison MR

更新日期：2008-06-01 00:00:00

abstract：:Proviral clones of human immunodeficiency virus type 1 which contained single amino acid changes in the envelope V3 region were constructed. PCR amplification of Sup-T1 T cells transfected with one such mutant, G312T, revealed low levels of virus that resulted in the generation of a revertant virus, in which an alanin...

journal_title：Journal of virology

authors： Morris JF,Sternberg EJ,Gutshall L,Petteway SR Jr,Ivanoff LA

更新日期：1994-12-01 00:00:00

abstract：:Claudins (CLDN) are a family of proteins that represent the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between epithelial cells. Several types of viruses make full use of CLDN to facilitate entry into cells....

journal_title：Journal of virology

authors： Ding G,Liu J,Shao Q,Wang B,Feng J,Li Y,Li L,Cao S,Cong F,Zhao Y,Liu S,Xiao Y

更新日期：2020-09-29 00:00:00

abstract：:Systemic application of first-generation adenovirus induces pathogenic effects in the liver. To begin unraveling the mechanisms underlying early liver toxicity after adenovirus infusion, particularly the role of macrophage activation and expression of viral genes in transduced target cells, first-generation adenovirus...

journal_title：Journal of virology

authors： Lieber A,He CY,Meuse L,Schowalter D,Kirillova I,Winther B,Kay MA

更新日期：1997-11-01 00:00:00

abstract：:The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus ...

journal_title：Journal of virology

authors： Jin H,Zhou H,Lu B,Kemble G

更新日期：2004-01-01 00:00:00

abstract：:This paper is concerned with the physiological role(s) of T4 phage-coded dihydrofolate reductase, which functions both in DNA precursor metabolism and as a virion protein. (i) We have detected enzyme activity in noninfectious particles produced under restrictive conditions by gene 11 mutants. This supports the conclus...

journal_title：Journal of virology

authors： Mosher RA,DiRenzo AB,Mathews CK

更新日期：1977-09-01 00:00:00

abstract：:F111 rat cells transformed by simian virus 40 mutant tsA1499 are cold sensitive for the expression of transformation. Yet, unlike F111 cells transformed by tsA58, they do not lose the ability to stabilize the transformation-associated host cell protein p53 at the temperature at which transformation is extinguished. ...

journal_title：Journal of virology

authors： Bouck N,Fikes J,Rundell MK

更新日期：1984-03-01 00:00:00

abstract：UNLABELLED:Nonhuman primates (NHPs) are a historically important source of zoonotic viruses and are a gold-standard model for research on many human pathogens. However, with the exception of simian immunodeficiency virus (SIV) (family Retroviridae), the blood-borne viruses harbored by these animals in the wild remain i...

journal_title：Journal of virology

authors： Bailey AL,Lauck M,Ghai RR,Nelson CW,Heimbruch K,Hughes AL,Goldberg TL,Kuhn JH,Jasinska AJ,Freimer NB,Apetrei C,O'Connor DH

更新日期：2016-07-11 00:00:00

abstract：:Widespread distribution of highly pathogenic avian H5N1 influenza viruses in domesticated and wild birds continues to pose a threat to public health, as interspecies transmission of virus has resulted in increasing numbers of human disease cases. Although the pathogenic mechanism(s) of H5N1 influenza viruses has not b...

journal_title：Journal of virology

authors： Szretter KJ,Gangappa S,Belser JA,Zeng H,Chen H,Matsuoka Y,Sambhara S,Swayne DE,Tumpey TM,Katz JM

更新日期：2009-06-01 00:00:00

abstract：:Multimerization of the Hantaan virus nucleocapsid protein (NP) in Hantaan virus-infected Vero E6 cells was observed in a competitive enzyme-linked immunosorbent assay (ELISA). Recombinant and truncated NPs of Hantaan, Seoul, and Dobrava viruses lacking the N-terminal 49 amino acids were also detected as multimers. Alt...

journal_title：Journal of virology

authors： Yoshimatsu K,Lee BH,Araki K,Morimatsu M,Ogino M,Ebihara H,Arikawa J

更新日期：2003-01-01 00:00:00

abstract：:HA20, a mutant of Bacillus subtilis phage SPO1, is deficient in both DNA replication and late transcription. HA20 contains mutations in two different genes, which suggested that the two effects might be caused independently. However, single-mutation derivatives, affected only in gene 27, were deficient for both activi...

journal_title：Journal of virology

authors： Stewart CR

更新日期：1984-01-01 00:00:00

abstract：:In 2010 and 2011, several devastating Newcastle disease (ND) outbreaks occurred in China, affecting broilers, layers, and breeders. The CK-JSX1-201005 virus was isolated from broiler breeder flocks vaccinated with the classical ND virus (NDV) vaccine program, but laying rate decreased from 80% to 30 to 40% in the clin...

journal_title：Journal of virology

authors： Chen F,Liu J,Liu D,Yan Z,Ji J,Qin J,Li H,Ma J,Bi Y,Xie Q

更新日期：2012-11-01 00:00:00

abstract：:Herpes simplex virus 1 (HSV-1) establishes a lifelong latent infection in host peripheral neurons, including the neurons of the trigeminal ganglia (TG). HSV-1 can reactivate from neurons to cause recurrent infection. During latency, the insulator protein CTCF occupies DNA binding sites on the HSV-1 genome, and these s...

journal_title：Journal of virology

authors： Washington SD,Edenfield SI,Lieux C,Watson ZL,Taasan SM,Dhummakupt A,Bloom DC,Neumann DM

更新日期：2018-05-14 00:00:00

abstract：:TR1.3 is a Friend murine leukemia virus (MLV) that induces selective syncytium induction (SI) of brain capillary endothelial cells (BCEC), intracerebral hemorrhage, and death. Syncytium induction by TR1.3 has been mapped to a single tryptophan-to-glycine conversion at position 102 of the envelope glycoprotein (Env102)...

journal_title：Journal of virology

authors： Murphy SL,Chung-Landers M,Honczarenko M,Gaulton GN

更新日期：2006-05-01 00:00:00

abstract：:mRNA's homologous to the herpes simplex virus type 1 DNA restriction endonuclease fragment BamHI p, which contains the thymidine kinase gene, have been identified and mapped by hybrid-arrested translation and mRNA selection. Such mRNA's, when translated in vitro, directed the synthesis of polypeptides of apparent mole...

journal_title：Journal of virology

authors： Preston CM,McGeoch DJ

更新日期：1981-05-01 00:00:00

abstract：:The full-length sequence of a satellite RNA (sat-RNA) of Beet black scorch virus isolate X (BBSV-X) was determined. This agent is 615 nucleotides long and lacks extensive sequence homology with its helper virus or with other reported viruses. Purified virus particles contained abundant single-stranded plus-sense monom...

journal_title：Journal of virology

authors： Guo LH,Cao YH,Li DW,Niu SN,Cai ZN,Han CG,Zhai YF,Yu JL

更新日期：2005-03-01 00:00:00

abstract：:In the course of our studies on oxidative stress as a component of pathological processes in humans, we showed that microintrusion into cells with microcapillary and ultramicroelectrochemical detection could mimic many types of mechanical intrusion leading to an instant (0.1 s) and high (some femtomoles) burst release...

journal_title：Journal of virology

authors： Lachgar A,Sojic N,Arbault S,Bruce D,Sarasin A,Amatore C,Bizzini B,Zagury D,Vuillaume M

更新日期：1999-02-01 00:00:00

abstract：:The uncoating of adenovirus type 2 and a temperature-sensitive mutant, tsl, was studied. HEp-2 cells were infected with 32P- OR 125I-labeled purified virions for various lengths of time, and the nuclear and cytoplasmic fractions were analyzed by sucrose gradient velocity sedimentation and sodium dodecyl sulfate-polyac...

journal_title：Journal of virology

authors： Mirza MA,Weber J

更新日期：1979-05-01 00:00:00

abstract：:The potential use of the modified vaccinia virus Ankara (MVA) strain as a live recombinant vector to deliver antigens and elicit protective immune responses against infectious diseases demands a comprehensive understanding of the effect of MVA infection on human host gene expression. We used microarrays containing mor...

journal_title：Journal of virology

authors： Guerra S,López-Fernández LA,Conde R,Pascual-Montano A,Harshman K,Esteban M

更新日期：2004-06-01 00:00:00

abstract：:RmI, a circular chimera made of the polyomavirus (Py) genome with an insertion of mouse DNA (Ins), effectively undergoes intramolecular recombination in normal mouse cells, as indicated by the conversion of cloned RmI (RmIc) into unit-length Py DNA in transfected cultures. To follow the fate of the cellular component ...

journal_title：Journal of virology

authors： Piché A,Bourgaux P

更新日期：1987-03-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) is a recently described arterivirus responsible for disease in swine worldwide. Comparative sequence analysis of 3'-terminal structural genes of the single-stranded RNA viral genome revealed the presence of two genotypic classes of PRRSV, represented by the p...

journal_title：Journal of virology

authors： Nelsen CJ,Murtaugh MP,Faaberg KS

更新日期：1999-01-01 00:00:00

abstract：:To investigate the cis-acting sequence elements that are involved in the regulation of herpes simplex virus type 1 late-gene expression, recombinant viruses were constructed that express the Escherichia coli lacZ gene from the promoter of the glycoprotein H (gH) gene. Deletion experiments established an upstream bound...

journal_title：Journal of virology

authors： Steffy KR,Weir JP

更新日期：1991-02-01 00:00:00

abstract：:Direct evidence is presented for a host-specific role of the cucumovirus capsid protein in long-distance movement within infected plants. Cucumber (Cucumis sativus L.) is a systemic host for cucumber mosaic cucumovirus (CMV). Tomato aspermy cucumovirus, strain 1 (1-TAV), multiplied to the levels of CMV (i.e., replicat...

journal_title：Journal of virology

authors： Taliansky ME,García-Arenal F

更新日期：1995-02-01 00:00:00

abstract：:We showed previously that retrovirus vector particles can encapsidate RNAs without retroviral cis-acting sequences, that such RNAs are reverse transcribed in infected target cells, and that the cDNA copies are inserted into the host genome resulting in cDNA genes (R. Dornburg and H. M. Temin, Mol. Cell. Biol. 8:2328-2...

journal_title：Journal of virology

authors： Dornburg R,Temin HM

更新日期：1990-02-01 00:00:00

abstract：:During paramyxovirus entry into a host cell, receptor engagement by a specialized binding protein triggers conformational changes in the adjacent fusion protein (F), leading to fusion between the viral and cell membranes. According to the existing paradigm of paramyxovirus membrane fusion, the initial activation of F ...

journal_title：Journal of virology

authors： Porotto M,Devito I,Palmer SG,Jurgens EM,Yee JL,Yokoyama CC,Pessi A,Moscona A

更新日期：2011-12-01 00:00:00

abstract：:Purified Kunjin virus was disrupted with sodium dodecyl sulfate, urea, and mercaptoethanol or acetic acid. Electrophoresis on 7.5% polyacrylamide gel separated four proteins of high (120,000), intermediate (65,000) and low (18,000 and 13,000) molecular weights. A "core" particle was obtained by degradation of the viri...

journal_title：Journal of virology

authors： Westaway EG,Reedman BM

更新日期：1969-11-01 00:00:00

abstract：:Initiation of reverse transcription of human immunodeficiency virus type 1 (HIV-1) occurs by extension from the 3' end of a cellular tRNA complexed to the primer binding site (PBS) located near the 5' end of the viral RNA genome. Although the PBSs for all naturally occurring HIV-1 viruses are complementary to the 3'-t...

journal_title：Journal of virology

authors： Kang SM,Zhang Z,Morrow CD

更新日期：1997-01-01 00:00:00