Abstract:
:This study identifies memory cytotoxic T lymphocyte (CTL) epitopes to respiratory syncytial virus (RSV) in healthy South African adults and demonstrates the conservation of those epitopes in circulating field strains of RSV in South Africa. Thirty-seven healthy adults from a population with diverse HLA backgrounds were screened by gamma interferon (IFN-gamma) enzyme-linked immunospot for memory CTL activity in response to overlapping peptides representing the complete nucleoprotein (N) of RSV. Responses of more than 40 spot-forming cells/million cells were detectable in 21 individuals. The significant responses were further characterized, and 14-mer peptides were identified that induced cytolytic activity. Fine mapping of peptides with the highest cytolytic activity identified an HLA-B(*)08-restricted RSV-specific CTL epitope. The extended 14-mer peptide containing this epitope also induced lysis in the context of A(*)02-restricted target cells in some individuals. These HLA types are common in the target population; thus, the epitope is useful for studies of CTL responses to RSV in humans. The epitope was detected in healthy adults, reflecting the response generated in the course of previous natural RSV infection. We obtained a large panel of naturally occurring isolates of RSV to determine whether there was evidence of escape from CTL activity in circulating strains. We found that this epitope and a previously identified B(*)07-restricted N protein epitope were conserved in RSV field strains representing the diversity of circulating genotypes. This work suggests that escape from CTL activity is not common for this acute respiratory infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Venter M,Rock M,Puren AJ,Tiemessen CT,Crowe JE Jrdoi
10.1128/jvi.77.13.7319-7329.2003subject
Has Abstractpub_date
2003-07-01 00:00:00pages
7319-29issue
13eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract::A hydrophilic region consisting of strikingly clustered charged amino acids is present at the center of human immunodeficiency virus type 1 (HIV-1) Vif. In this study, the role for this central hydrophilic region (E(88)WRKKR(93)) in the virus replication in nonpermissive H9 cells was investigated by extensive deletion...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1626-1632.2003
更新日期:2003-01-01 00:00:00
abstract::Monoclonal antibodies (MAbs) to human immunodeficiency virus type 1 were produced. Two antibodies reacted with the 17-kilodalton core protein (p17) of the virus and with its polyprotein precursor. To various degrees, each MAb neutralized infection by the cell-free virus. With a series of sequential overlapping hexapep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.267-272.1989
更新日期:1989-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02637-09
更新日期:2010-05-01 00:00:00
abstract::Interleukin-15 (IL-15), natural killer (NK) cells, and NK T (NKT) cells, components of the innate immune system, are known to contribute to defense against pathogens, including viruses. Here we report that IL-15(-/-) (NK(-) and NKT(-/+)) mice and RAG-2(-/-)/gamma(c)(-/-) (NK(-) and NKT(-)) mice that lack all lymphoid ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.10168-10171.2003
更新日期:2003-09-01 00:00:00
abstract::The entry of the viral genomic DNA of cauliflower mosaic virus into the nucleus is a critical step of viral infection. We have shown by transient expression in plant protoplasts that the viral coat protein (CP), which is processed from the product of open reading frame IV, contains an N-terminal nuclear localization s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.553-560.1999
更新日期:1999-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3746-3758.1991
更新日期:1991-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.125-139.1974
更新日期:1974-01-01 00:00:00
abstract::DNA replication intermediates of three plasmids containing all or part of a modified Epstein-Barr virus cis-acting plasmid maintenance region (oriP) were examined to further investigate oriP function. Replication intermediates were analyzed in vivo and in vitro by neutral-neutral two-dimensional gel electrophoresis. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1739-1745.1993
更新日期:1993-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02911-15
更新日期:2016-04-14 00:00:00
abstract::Hypoxia-inducible factor 1 (HIF-1) is a ubiquitously expressed transcriptional regulator involved in induction of numerous genes associated with angiogenesis and tumor growth. Kaposi's sarcoma, associated with increased angiogenesis, is a highly vascularized, endothelial cell-derived tumor. Previously, we have shown t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00611-07
更新日期:2007-10-01 00:00:00
abstract::Recent environmental and metagenomic studies have considerably increased the repertoire of archaeal viruses and suggested that they play important roles in nutrient cycling in the biosphere. However, very little is known about how they regulate their life cycles and interact with their hosts. Here, we report that the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00841-20
更新日期:2020-07-30 00:00:00
abstract::Major neutralization antigenic sites have been previously mapped by us on VP1, the largest capsid protein of poliovirus type 1. Here we report the first identification of the primary sequence of a neutralization antigenic site on capsid protein VP2. Inspection of the amino acid sequence of VP2 led to the selection and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.719-721.1984
更新日期:1984-11-01 00:00:00
abstract::Cytolytic T-lymphocyte (CTL) activity specific for respiratory syncytial (RS) virus was investigated after intranasal infection of mice with RS virus, after intraperitoneal infection of mice with a recombinant vaccinia virus expressing the F glycoprotein, and after intramuscular vaccination of mice with Formalin-inact...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4232-4241.1990
更新日期:1990-09-01 00:00:00
abstract::TMC125 is a potent new investigational nonnucleoside reverse transcriptase inhibitor (NNRTI) that is active against human immunodeficiency virus type 1 (HIV-1) with resistance to currently licensed NNRTIs. Sequential passage experiments with both wild-type virus and NNRTI-resistant virus were performed to identify mut...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12773-12782.2005
更新日期:2005-10-01 00:00:00
abstract::Bicistronic RNAs containing the 373-nucleotide-long 5' nontranslated region (NTR) of the classical swine fever virus (CSFV) genome as intercistronic spacer were used to show the presence of an internal ribosome entry site (IRES) in the 5' end of the CSFV genome. By coexpression of the poliovirus 2A protease it was dem...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.451-457.1997
更新日期:1997-01-01 00:00:00
abstract::Infection of cells with herpes simplex virus type 1 (HSV-1) results in a rapid alteration of phosphorylation on the large subunit of cellular RNA polymerase II (RNAP II), most likely on its C-terminal domain (S. A. Rice, M. C. Long, V. Lam, C. A. Spencer, J. Virol. 68:988-1001, 1994). This phosphorylation modification...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5550-5559.1995
更新日期:1995-09-01 00:00:00
abstract::Natural killer T (NKT) cells are unique T lymphocytes that recognize CD1d-bound lipid antigens and play an important role in both innate and acquired immune responses against infectious diseases and tumors. We have already shown that a vesicular stomatitis virus (VSV) infection results in the rapid inhibition of murin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00881-08
更新日期:2008-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2255-2265.1993
更新日期:1993-04-01 00:00:00
abstract::Three late transcripts have been mapped to the vaccinia virus 7.1-kilobase (kb) EcoRI F fragment, which is located approximately 85 kb from the left end of the viral genome. Hybrid selection and translation of RNA have demonstrated that these mRNAs encode three polypeptides with apparent molecular weights of 32,000 (3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.510-520.1984
更新日期:1984-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-01-17 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6626-6633.1999
更新日期:1999-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.3.1228-1234.1983
更新日期:1983-03-01 00:00:00
abstract::Single-envelope human immunodeficiency virus (HIV) vaccines have been studied for more than a decade, with some successes in homologous challenge experiments in nonhuman primates but with no clear successes in clinical trials. To gain insight into the breadth of the immunity elicited by such vaccines, we have dissecte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4231-4236.2003
更新日期:2003-04-01 00:00:00
abstract::Spanins are bacteriophage lysis proteins responsible for disruption of the outer membrane, the final step of Gram-negative host lysis. The absence of spanins results in a terminal phenotype of fragile spherical cells. The phage T1 employs a unimolecular spanin gp11 that has an N-terminal lipoylation signal and a C-ter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00380-18
更新日期:2018-10-29 00:00:00
abstract::HIV-1 circulates within an infected host as a genetically heterogeneous viral population. Viral intrahost diversity is shaped by substitutional evolution and recombination. Although many studies have speculated that recombination could have a significant impact on viral phenotype, this has never been definitively demo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00131-11
更新日期:2011-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8690-8696.1998
更新日期:1998-11-01 00:00:00
abstract::We previously reported that human cytomegalovirus (HCMV) infection induces endoplasmic reticulum (ER) stress, resulting in activation of the unfolded protein response (UPR). Although some normal consequences of UPR activation (e.g., translation attenuation) are detrimental to viral infection, we have previously shown ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15388-15397.2005
更新日期:2005-12-01 00:00:00
abstract::The Epstein-Barr virus (EBV) is an oncogenic human herpesvirus. EBV latent membrane protein 1 (LMP-1) is a viral oncogene that manifests its oncogenic phenotype through activation of cellular signaling pathways involved in cell growth, survival, differentiation, and transformation. Lytic LMP-1 (lyLMP-1) is a related E...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00180-06
更新日期:2006-08-01 00:00:00
abstract::We have examined the pattern of RNA transcription on the L (late) DNA strand late in the simian virus 40 virus infectious cycle by separating pulse-labeled RNA chains according to size and hybridizing to an ordered series of DNA fragments obtained by restriction enzyme digestion. From this analysis, the 5' end of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.795-801.1978
更新日期:1978-12-01 00:00:00
abstract::The varicella-zoster virus (VZV) open reading frame 54 (ORF54) gene encodes an 87-kDa monomer that oligomerizes to form the VZV portal protein, pORF54. pORF54 was hypothesized to perform a function similar to that of a previously described herpes simplex virus 1 (HSV-1) homolog, pUL6. pUL6 and the associated viral ter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00376-14
更新日期:2014-07-01 00:00:00