Abstract:
:Bicistronic RNAs containing the 373-nucleotide-long 5' nontranslated region (NTR) of the classical swine fever virus (CSFV) genome as intercistronic spacer were used to show the presence of an internal ribosome entry site (IRES) in the 5' end of the CSFV genome. By coexpression of the poliovirus 2A protease it was demonstrated that the CSFV 5' NTR-driven translation is independent of the presence of functional eukaryotic initiation factor eIF-4F. Deletion analysis indicated that the 5' border of the IRES is located between nucleotides 28 and 66. The role of a proposed pseudoknot structure at the 3' end of the CSFV 5' NTR in IRES-mediated translation was investigated by site-directed mutagenesis. Mutant RNAs that had lost the ability to base pair in stem II of the pseudoknot were translationally inactive. Translation to wild-type levels could be restored through the introduction of compensatory complementary base changes that repaired base pairing in stem II. In addition, we showed that the AUG codon, which is located 7 nucleotides upstream of the polyprotein initiation site and is conserved in pestiviruses, could not be used to initiate translation. Also, an AUG codon introduced downstream of the polyprotein initiation site was not recognized as an initiation site by ribosomes. These data suggest that after internal entry on the CSFV 5' NTR, ribosomal scanning for the initiation codon is limited to a small region.
journal_name
J Viroljournal_title
Journal of virologyauthors
Rijnbrand R,van der Straaten T,van Rijn PA,Spaan WJ,Bredenbeek PJdoi
10.1128/JVI.71.1.451-457.1997subject
Has Abstractpub_date
1997-01-01 00:00:00pages
451-7issue
1eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::The B19 parvovirus, a small single-stranded DNA virus of 5.4 kilobases, is pathogenic in humans. B19 has remarkable specificity for erythroid progenitor cells and has been propagated in vitro only with human erythroid bone marrow. Replication of viral DNA and the viral protein products of B19 appear similar to those o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2395-2406.1987
更新日期:1987-08-01 00:00:00
abstract::Treatment of patients with lamivudine (3TC) results in loss of detectable levels of hepatitis B virus (HBV) DNA from serum; however, the relapse rate, with regard to both reappearance of virus in the bloodstream and hepatic inflammation, is high when therapy is terminated. Although the rebound observed in patients has...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8148-8160.2002
更新日期:2002-08-01 00:00:00
abstract::Receptors (FcgammaRs) for the constant region of immunoglobulin G (IgG) are an important link between humoral immunity and cellular immunity. To help define the role of FcgammaRs in determining the fate of human immunodeficiency virus type 1 (HIV-1) immune complexes, cDNAs for the four major human Fcgamma receptors (F...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00656-09
更新日期:2009-08-01 00:00:00
abstract::Flavopiridol, roscovitine, and other inhibitors of Cyclin-Dependent Kinases (CDK) inhibit the replication of a variety of viruses in vitro while proving nontoxic in human clinical trials of their effects against cancer. Consequently, these and other Pharmacological CDK inhibitors (PCIs) have been proposed as potential...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9352-9365.2004
更新日期:2004-09-01 00:00:00
abstract::HVEM (for herpesvirus entry mediator) is a member of the tumor necrosis factor receptor superfamily and mediates entry of many strains of herpes simplex virus (HSV) into normally nonpermissive Chinese hamster ovary (CHO) cells. We used sucrose density centrifugation to demonstrate that purified HSV-1 KOS virions bind ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3595-3601.1998
更新日期:1998-05-01 00:00:00
abstract:UNLABELLED:The four serotypes of dengue virus (DENV) cause the most important and rapidly emerging arboviral diseases in humans. The recent phase 2b and 3 studies of a tetravalent dengue vaccine reported a moderate efficacy despite the presence of neutralizing antibodies, highlighting the need for a better understandin...
journal_title:Journal of virology
pub_type: 杂志文章,随机对照试验
doi:10.1128/JVI.00273-15
更新日期:2015-07-01 00:00:00
abstract::A porcine rotavirus (prv), variant 4F, isolated in tissue culture from the feces of a Chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (J. C. Bridger, B. Burke, G. M. Beards, and U. Desselberger, J. Gen. Virol. 73:3011-3015, 1992). Comparison with the closely r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.853-857.1997
更新日期:1997-01-01 00:00:00
abstract::Humans chronically infected with hepatitis B virus (HBV) are at further risk of liver cancer upon exposure to dietary aflatoxin B1 (AFB1), a carcinogenic product of the mold Aspergillus flavus. For the present study, we utilized double-transgenic mice (ATX mice) that express the HBV X protein (HBx) and possess a bacte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11770-11774.2002
更新日期:2002-11-01 00:00:00
abstract::Viral RNA-dependent RNA polymerases exhibit great sequence diversity. Only six core amino acids are conserved across all polymerases of positive-strand RNA viruses of eukaryotes. While exploring the function of one of these completely conserved residues, asparagine 297 in the prototypic poliovirus polymerase 3D(pol), ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.9.5378-5388.2003
更新日期:2003-05-01 00:00:00
abstract::The morphogenesis of vesicular stomatitis virus was examined using freeze-fracture techniques, and the results obtained were compared with those from previously published experiments carried out with influenza viruses and togaviruses. The process of conversion of the host cell plasma membrane into the vesicular stomat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.2.601-609.1977
更新日期:1977-02-01 00:00:00
abstract::HLA-B*57 is strongly associated with immune control of HIV and delayed AIDS progression. The closely related, but less protective, HLA-B*58:01 presents similar epitopes, but HLA-B*58:01(+) individuals do not generate CD8(+) T cells targeting the KF11-Gag epitope, which has been linked to low viremia. Here we show that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01122-13
更新日期:2013-10-01 00:00:00
abstract::Respiratory syncytial virus (RSV) produces intense pulmonary inflammation, in part through its ability to induce chemokine synthesis in infected airway epithelial cells. RANTES (regulated upon activation, normally T-cell expressed and presumably secreted) is a CC chemokine which recruits and activates monocytes, lymph...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6428-6439.2001
更新日期:2001-07-01 00:00:00
abstract::The complete genome sequences of two Sulfolobus spindle-shaped viruses (SSVs) from acidic hot springs in Kamchatka (Russia) and Yellowstone National Park (United States) have been determined. These nonlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both viruses share the spindle-shap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1954-1961.2004
更新日期:2004-02-01 00:00:00
abstract:UNLABELLED:The porcine sapovirus (SaV) (PoSaV) Cowden strain is one of only a few culturable enteric caliciviruses. Compared to the wild-type (WT) PoSaV Cowden strain, tissue culture-adapted (TC) PoSaV has two conserved amino acid substitutions in the RNA-dependent RNA polymerase (RdRp) and six in the capsid protein (V...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02197-15
更新日期:2015-11-18 00:00:00
abstract::Chikungunya virus (CHIKV) is a mosquito-borne alphavirus responsible for chikungunya fever. Nonstructural protein 2 (nsP2), a multifunctional protein essential for viral replication, has an N-terminal helicase region (nsP2h), which as both nucleotide triphosphatase and RNA triphosphatase activities, as well as a C-ter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01470-20
更新日期:2020-12-16 00:00:00
abstract::Recent studies have proposed that adeno-associated viruses (AAVs) are not evolutionarily linked to other mammalian autonomous parvoviruses but are more closely linked to the autonomous parvoviruses of birds. To better understand the relationship between primate and avian AAVs (AAAVs), we cloned and sequenced the genom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.12.6799-6810.2003
更新日期:2003-06-01 00:00:00
abstract::To combat emerging coronaviruses, developing safe and efficient platforms to evaluate viral protease activities and the efficacy of protease inhibitors is a high priority. Here, we exploit a biosafety level 2 (BSL-2) chimeric Sindbis virus system to evaluate protease activities and the efficacy of inhibitors directed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01749-14
更新日期:2014-10-01 00:00:00
abstract::BALB/c mice treated with pristane and Abelson virus have been used as an animal model system for the rapid induction of plasmacytomas. Myelomonocytic tumors with helper Moloney murine leukemia virus clonally inserted into the c-myb locus were observed in about 10% of pristane-primed BALB/c mice infected with Abelson v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3721-3725.1987
更新日期:1987-12-01 00:00:00
abstract::To study the variability of human immunodeficiency virus type 1 (HIV-1), we used immunotoxins to select for variants within a population of H9 cells persistently infected with a molecular clone of HIV-1 designated NL4-3. Chimeric immunotoxin CD4-PE40 (a chimeric fusion protein consisting of the amino-terminal two doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.75-81.1995
更新日期:1995-01-01 00:00:00
abstract::Retroviruses are believed to induce tumors by acting as insertional mutagens that activate expression of cellular protooncogenes. Indeed, almost 90% of mouse mammary tumor virus (MMTV)-induced mammary tumors in C3H/He mice show upregulation of Int protooncogenes. We have analyzed three different MMTV variants [MMTV(C3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.8876-8883.2000
更新日期:2000-10-01 00:00:00
abstract::Pestiviruses prevent alpha/beta interferon (IFN-alpha/beta) production by promoting proteasomal degradation of interferon regulatory factor 3 (IRF3) by means of the viral N(pro) nonstructural protein. N(pro) is also an autoprotease, and its amino-terminal coding sequence is involved in translation initiation. We previ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01509-08
更新日期:2009-01-01 00:00:00
abstract::The foot-and-mouth disease virus (FMDV) capsid is highly acid labile, but introduction of amino acid replacements, including an N17D change in VP1, can increase its acid resistance. Using mutant VP1 N17D as a starting point, we isolated a virus with higher acid resistance carrying an additional replacement, VP2 H145Y,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03222-13
更新日期:2014-03-01 00:00:00
abstract::Two major DNA variants have been isolated from serially propagated stocks of the WR strain of vaccinia virus. Restriction enzyme mapping of the two variants with HindIII, AvaI, XhoI, SstII, and SmaI revealed a 6.3-megadalton deletion in the smaller DNA variant. The deletion was mapped at ca. 6.8 megadaltons in from th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.1000-1010.1981
更新日期:1981-03-01 00:00:00
abstract::A single phosphorylation event at T-antigen residue Thr124 regulates initiation of simian virus 40 DNA replication. To explore this regulatory process, a series of peptides were synthesized, centered on Thr124. These peptides contain a nuclear localization signal (NLS) and a recognition site for cyclin/Cdk kinases. Wh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.11785-11792.2002
更新日期:2002-12-01 00:00:00
abstract::Although the genetic organization of tobacco mosaic virus (TMV) differs considerably from that of the tripartite viruses (alfalfa mosaic virus [AlMV] and brome mosaic virus [BMV]), all of these RNA plant viruses share three domains of homology among their nonstructural proteins. One such domain, common to the AlMV and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.536-542.1985
更新日期:1985-02-01 00:00:00
abstract:UNLABELLED:Hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) are critical in eliminating infection. We developed an animal model in which HBV-infected human hepatocytes are targeted by HBV-specific CTLs. After HBV inoculation in human hepatocyte-transplanted herpes simplex virus type-1 thymidine kinase-NO...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01126-15
更新日期:2015-10-01 00:00:00
abstract::F proteins from baculovirus nucleopolyhedrovirus (NPV) group II members are the major budded virus (BV) viral envelope fusion proteins. They undergo furin-like proteolysis processing in order to be functional. F proteins from different baculovirus species have a long cytoplasmic tail domain (CTD), ranging from 48 (Spo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01178-06
更新日期:2006-11-01 00:00:00
abstract::Highly conserved amino acids in the second helix structure of the human immunodeficiency virus type 1 (HIV-1) MA protein were identified to be critical for the incorporation of viral Env proteins into HIV-1 virions from transfected COS-7 cells. The effects of these MA mutations on viral replication in the HIV-1 natura...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1443-1452.1997
更新日期:1997-02-01 00:00:00
abstract::Herpes simplex virus type 1 glycoprotein H (HSV-1 gH) was synthesized in an inducible mammalian cell expression system, and its properties were examined. The gH coding sequence, together with the stable 5' untranslated leader sequence from xenopus beta-globin, was placed under control of the strong promoter from the h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4744-4755.1989
更新日期:1989-11-01 00:00:00
abstract::Bacteriophages of the C3 morphotype, characterized by very long heads that exceed their width several times, are extremely rare among the Podoviridae family members and constitute only 0.5% of over 5,500 phages that have been examined by the electron microscope (H. W. Ackermann, Arch. Virol. 152:227-243, 2007; H. W. A...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02727-12
更新日期:2012-12-01 00:00:00