Abstract:
:The entry of the viral genomic DNA of cauliflower mosaic virus into the nucleus is a critical step of viral infection. We have shown by transient expression in plant protoplasts that the viral coat protein (CP), which is processed from the product of open reading frame IV, contains an N-terminal nuclear localization signal (NLS). The NLS is exposed on the surface of the virion and is thus available for interaction with a putative NLS receptor. Phosphorylation of the matured CP did not influence the nuclear localization of the protein but improved protein stability. Mutation of the NLS completely abolished viral infectivity, thus indicating its importance in the virus life cycle. The NLS seems to be regulated by the N terminus of the precapsid, which inhibits its nuclear targeting. This regulation could be important in allowing virus assembly in the cytoplasm.
journal_name
J Viroljournal_title
Journal of virologyauthors
Leclerc D,Chapdelaine Y,Hohn Tdoi
10.1128/JVI.73.1.553-560.1999subject
Has Abstractpub_date
1999-01-01 00:00:00pages
553-60issue
1eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
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