Abstract:
:Temperature-sensitive (ts) mutants were isolated from the baculovirus Autographa californica (alfalfa looper) MNPV, grown in Spodoptera frugiperda (fall armyworm) cells in the presence of N-methyl-N'-nitro-N-nitrosoguanidine. Of 567 plaque isolates screened, 27 were temperature sensitive (ts), representing a mutation frequency of 4.8%. Ten ts mutants were studied in detail: six failed to yield nonoccluded virus at 33 degrees C (NOV mutants), whereas the other four produced nonoccluded virus but were restricted in formation of polyhedra at 33 degrees C (Poly mutants). One of the six NOV mutants failed to synthesize viral DNA. Reversion and leak frequencies were determined, and the mutants were assorted into complementation groups based on the yield of polyhedrin synthesis in cells coinfected with pairs of mutants at 33 degrees C, as measured by radioimmunoassay. For NOV mutants, complementation indexes were also based on virus yield and were consistent with those based on polyhedrin synthesis. Nine mutants were assorted into five complementation groups. One mutant remained unclassified.
journal_name
J Viroljournal_title
Journal of virologyauthors
Brown M,Crawford AM,Faulkner Pdoi
10.1128/JVI.31.1.190-198.1979subject
Has Abstractpub_date
1979-07-01 00:00:00pages
190-8issue
1eissn
0022-538Xissn
1098-5514journal_volume
31pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.69.11.7264-7268.1995
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pub_type: 杂志文章
doi:10.1128/JVI.67.9.5216-5225.1993
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pub_type: 杂志文章
doi:10.1128/JVI.60.2.363-368.1986
更新日期:1986-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5363-5372.1992
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.72.12.9844-9854.1998
更新日期:1998-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3392-3400.1996
更新日期:1996-06-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00