Abstract:
:Early stages of infection by the mouse polyomavirus have been studied using HeLa cells stably expressing small interfering RNA to protein disulfide isomerase (PDI). Infectibility measured by nuclear T antigen expression was reduced commensurately with the degree of PDI downregulation. Infectibility was restored by transfection with a plasmid expressing PDI but not with a control expressing catalytically inactive enzyme. Deconvolution microscopy using fluorescently labeled virus and cellular markers showed that virus reaches the endoplasmic reticulum (ER) normally in cells with reduced PDI but subsequently fails to exit the ER. Simian virus 40 infection was not inhibited in PDI-downregulated cells. The results are discussed in terms of structural differences between the two viruses and current knowledge of virus disassembly in the ER.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gilbert J,Ou W,Silver J,Benjamin Tdoi
10.1128/JVI.01117-06subject
Has Abstractpub_date
2006-11-01 00:00:00pages
10868-70issue
21eissn
0022-538Xissn
1098-5514pii
JVI.01117-06journal_volume
80pub_type
杂志文章abstract::Retroviruses are believed to induce tumors by acting as insertional mutagens that activate expression of cellular protooncogenes. Indeed, almost 90% of mouse mammary tumor virus (MMTV)-induced mammary tumors in C3H/He mice show upregulation of Int protooncogenes. We have analyzed three different MMTV variants [MMTV(C3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.8876-8883.2000
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abstract::Different isolates of human immunodeficiency virus type 1 (HIV-1) vary in the cell tropisms they display, i.e., the range of cell types in which they are able to establish a productive infection. Here, we report on the phenotypes of recombinants between two molecularly cloned strains of HIV-1. Our results prove that t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.1.305-309.1992
更新日期:1992-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3255-3258.1997
更新日期:1997-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.903-913.1981
更新日期:1981-09-01 00:00:00
abstract::The amino-terminal region of the Vif molecule in human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus (SIV) contains a conserved SLV/Ix4Yx9Y motif that was first described in 1992, but the importance of this motif for Vif function has not yet been examined. Our characterization of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00653-09
更新日期:2009-09-01 00:00:00
abstract::In gene therapy, the innate immune system is a significant barrier to the effective application of adenovirus (Ad) vectors. In kidney epithelium-derived (REC) cells, serotype 5 Ad vectors induce the expression of the chemokine CXCL10 (IP-10), a response that is dependent on NFkappaB. Compared to the parental vector Ad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14507-14515.2005
更新日期:2005-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.129-136.1989
更新日期:1989-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.207-218.1981
更新日期:1981-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02349-06
更新日期:2007-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3924-3928.2000
更新日期:2000-04-01 00:00:00
abstract::"L-cell virion," obtained from chronically infected 929 L-cell lines, was purified on 70% sucrose pads and its ribonucleic acid (RNA) was extracted and analyzed on sucrose gradients. Two RNA components were isolated from the virion, a rapidly sedimenting species (82S) and a more slowly sedimenting species (6S). When t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.1.31-35.1969
更新日期:1969-07-01 00:00:00
abstract::RNA editing of the hepatitis delta virus (HDV) antigenome at the amber/W site by the host RNA adenosine deaminase ADAR1 is a critical step in the HDV replication cycle. Editing is required for production of the viral protein hepatitis delta antigen long form (HDAg-L), which is necessary for viral particle production b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11187-11193.2005
更新日期:2005-09-01 00:00:00
abstract::In vivo, the inferred circular retrovirus DNA precursor to the provirus contains two long terminal repeats (LTRs) in tandem. We studied the site-specific nicking of supercoiled DNA that contains tandem copies of avian retrovirus LTR DNA in vitro by using purified avian myeloblastosis virus pp32 endonuclease, Mg2+, and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.3.970-974.1986
更新日期:1986-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.585-598.1975
更新日期:1975-03-01 00:00:00
abstract::Hepatitis delta virus (HDV) contains a circular RNA genome of 1.7 kb. HDV RNA replication is thought to proceed via a rolling-circle mechanism that is dependent on autocatalytic cleavage and ligation reactions. However, it has never been established that these ribozyme activities are indeed involved in HDV RNA replica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2228-2234.1993
更新日期:1993-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01011-08
更新日期:2008-10-01 00:00:00
abstract::By using temperature-sensitive (ts) and suppressor-sensitive (sus) mutants, 11 essential genes have been identified in phage phi105. The order of the genes has been established in two- and three-factor crosses. The genes can be arranged in a linear order; this order is identical in the vegetative phage and in the prop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.6.760-767.1970
更新日期:1970-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7907-7911.1999
更新日期:1999-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.12646-12653.2002
更新日期:2002-12-01 00:00:00
abstract::The present study was designed to investigate the endocytic pathway involved in canine parvovirus (CPV) infection. Reduced temperature (18 degrees C) or the microtubule-depolymerizing drug nocodazole was found to inhibit productive infection of canine A72 cells by CPV and caused CPV to be retained in cytoplasmic vesic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.802-806.1998
更新日期:1998-01-01 00:00:00
abstract::Understanding how HIV-1 persists during effective antiretroviral therapy (ART) should inform strategies to cure HIV-1 infection. We hypothesize that proliferation of HIV-1-infected cells contributes to persistence of HIV-1 infection during suppressive ART. This predicts that identical or monotypic HIV-1 DNA sequences ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01985-12
更新日期:2013-02-01 00:00:00
abstract::The assembly of an orthoretrovirus such as HIV-1 requires the coordinated functioning of multiple biochemical activities of the viral Gag protein. These activities include membrane targeting, lattice formation, packaging of the RNA genome, and recruitment of cellular cofactors that modulate assembly. In most previous ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01844-19
更新日期:2020-02-14 00:00:00
abstract::Theiler's virus-induced demyelinating disease results from a chronic infection in the white matter of the central nervous system and provides an excellent model for human multiple sclerosis. Like multiple sclerosis, there are genetic risk factors in disease development, including genes associated with the major histoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.686-690.1990
更新日期:1990-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.3.1023-1026.1985
更新日期:1985-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11754-11766.2003
更新日期:2003-11-01 00:00:00
abstract::TT virus (TTV) is a recently identified widespread DNA virus of humans that produces persistent viremia in the absence of overt clinical manifestations. In an attempt to shed light on the dynamics of chronic infection, we measured the levels of TTV in the plasma of 25 persistently infected patients during the first 3 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.24.11999-12004.2001
更新日期:2001-12-01 00:00:00
abstract::Protein kinase activity in general is stimulated at least 5- to 10-fold in ribosomal salt wash preparations from poliovirus-infected HeLa cells compared with those from mock-infected cells. The stimulation of kinase activity is manifested by increased phosphorylation of ribosome-associated polypeptides having approxim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.6.1781-1787.1987
更新日期:1987-06-01 00:00:00