Dominant-negative inhibition of prion formation diminished by deletion mutagenesis of the prion protein.

Abstract:

:Polymorphic basic residues near the C terminus of the prion protein (PrP) in humans and sheep appear to protect against prion disease. In heterozygotes, inhibition of prion formation appears to be dominant negative and has been simulated in cultured cells persistently infected with scrapie prions. The results of nuclear magnetic resonance and mutagenesis studies indicate that specific substitutions at the C-terminal residues 167, 171, 214, and 218 of PrP(C) act as dominant-negative, inhibitors of PrP(Sc) formation (K. Kaneko et al., Proc. Natl. Acad. Sci. USA 94:10069-10074, 1997). Trafficking of substituted PrP(C) to caveaola-like domains or rafts by the glycolipid anchor was required for the dominant-negative phenotype; interestingly, amino acid replacements at multiple sites were less effective than single-residue substitutions. To elucidate which domains of PrP(C) are responsible for dominant-negative inhibition of PrP(Sc) formation, we analyzed whether N-terminally truncated PrP(Q218K) molecules exhibited dominant-negative effects in the conversion of full-length PrP(C) to PrP(Sc). We found that the C-terminal domain of PrP is not sufficient to impede the conversion of the full-length PrP(C) molecule and that N-terminally truncated molecules (with residues 23 to 88 and 23 to 120 deleted) have reduced dominant-negative activity. Whether the N-terminal region of PrP acts by stabilizing the C-terminal domain of the molecule or by modulating the binding of PrP(C) to an auxiliary molecule that participates in PrP(Sc) formation remains to be established.

journal_name

J Virol

journal_title

Journal of virology

authors

Zulianello L,Kaneko K,Scott M,Erpel S,Han D,Cohen FE,Prusiner SB

doi

10.1128/jvi.74.9.4351-4360.2000

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

4351-60

issue

9

eissn

0022-538X

issn

1098-5514

journal_volume

74

pub_type

杂志文章
  • Cell-associated West Nile flavivirus is covered with E+pre-M protein heterodimers which are destroyed and reorganized by proteolytic cleavage during virus release.

    abstract::Flaviviruses are enveloped viruses which accumulate in cellular vacuoles prior to release. The membrane of cell-associated virus contains the proteins pre-M and E. During release of virus the pre-M protein is cleaved, and only its carboxy-terminal segment remains associated with the virus as M protein. Studies of the ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.63.6.2521-2526.1989

    authors: Wengler G,Wengler G

    更新日期:1989-06-01 00:00:00

  • Phenotypic and functional alterations of dendritic cells induced by human herpesvirus 6 infection.

    abstract::Human herpesvirus 6 (HHV-6) has a tropism for T lymphocytes and monocytes/macrophages, suggesting that HHV-6 infection affects the immunosurveillance system. In the present study, we investigated the HHV-6-induced phenotypic and functional alterations of dendritic cells (DCs), which are professional antigen-presenting...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.20.10338-10345.2002

    authors: Kakimoto M,Hasegawa A,Fujita S,Yasukawa M

    更新日期:2002-10-01 00:00:00

  • Human immunodeficiency virus type 1 nucleocapsid protein can prevent self-priming of minus-strand strong stop DNA by promoting the annealing of short oligonucleotides to hairpin sequences.

    abstract::Understanding how viral components collaborate to convert the human immunodeficiency virus type 1 genome from single-stranded RNA into double-stranded DNA is critical to the understanding of viral replication. Not only must the correct reactions be carried out, but unwanted side reactions must be avoided. After minus-...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.19.8785-8792.2000

    authors: Driscoll MD,Hughes SH

    更新日期:2000-10-01 00:00:00

  • Rotavirus-induced structural and functional alterations in tight junctions of polarized intestinal Caco-2 cell monolayers.

    abstract::We provide here new insights into rotavirus (RRV) pathogenicity by showing that RRV infection promotes structural and functional injuries localized at the tight junctions (TJ) in the cell-cell junctional complex of cultured polarized human intestinal Caco-2 cells forming monolayers. RRV infection resulted in a progres...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.10.4645-4651.2000

    authors: Obert G,Peiffer I,Servin AL

    更新日期:2000-05-01 00:00:00

  • Evidence for persistent, occult infection in neonatal macaques following perinatal transmission of simian-human immunodeficiency virus SF162P3.

    abstract::To model human immunodeficiency virus (HIV) perinatal transmission, we studied infection of simian-human immunodeficiency virus (SHIV) SF162P3 in 10 pregnant Macaca nemestrina females and their offspring. Four of nine infants born to and suckled by these dams had evidence of infection, a transmission rate of 44.4% (95...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01759-06

    authors: Jayaraman P,Zhu T,Misher L,Mohan D,Kuller L,Polacino P,Richardson BA,Bielefeldt-Ohmann H,Anderson D,Hu SL,Haigwood NL

    更新日期:2007-01-01 00:00:00

  • Identification and transcriptional analyses of the UL3 and UL4 genes of equine herpesvirus 1, homologs of the ICP27 and glycoprotein K genes of herpes simplex virus.

    abstract::The DNA sequence of 3,240 nucleotides of the XbaI G fragment located in the unique long (UL) region of the equine herpesvirus 1 genome revealed two major open reading frames (ORFs) designated UL3 and UL4. The UL3 ORF of 470 amino acids (aa) maps at nucleotides (nt) 4450 to 3038 from the long terminus, and its predicte...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.9.5363-5372.1992

    authors: Zhao Y,Holden VR,Harty RN,O'Callaghan DJ

    更新日期:1992-09-01 00:00:00

  • Defining the structure-function relationships of bluetongue virus helicase protein VP6.

    abstract::The VP6 protein of bluetongue virus possesses a number of activities, including nucleoside triphosphatase, RNA binding, and helicase activity (N. Stauber, J. Martinez-Costas, G. Sutton, K. Monastyrskaya, and P. Roy, J. Virol. 71:7220-7226, 1997). Although the enzymatic functions of the protein have been documented, a ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.21.11347-11356.2003

    authors: Kar AK,Roy P

    更新日期:2003-11-01 00:00:00

  • Covalently closed circular DNA is the predominant form of duck hepatitis B virus DNA that persists following transient infection.

    abstract::Residual hepatitis B virus (HBV) DNA can be detected in serum and liver after apparent recovery from transient infection. However, it is not known if this residual HBV DNA represents ongoing viral replication and antigen expression. In the current study, ducks inoculated with duck hepatitis B virus (DHBV) were monitor...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.19.12242-12252.2005

    authors: Le Mire MF,Miller DS,Foster WK,Burrell CJ,Jilbert AR

    更新日期:2005-10-01 00:00:00

  • CREB-binding protein and histone deacetylase regulate the transcriptional activity of Kaposi's sarcoma-associated herpesvirus open reading frame 50.

    abstract::Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) open reading frame 50 (ORF50) encodes a viral transcriptional activator, which binds to the KSHV promoter and stimulates the transcription of viral early and late genes, thus activating the lytic cycle of KSHV. We report here that KSHV ORF50 binds to the cellular pro...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.4.1909-1917.2001

    authors: Gwack Y,Byun H,Hwang S,Lim C,Choe J

    更新日期:2001-02-01 00:00:00

  • Soluble Epstein-Barr virus glycoproteins gH, gL, and gp42 form a 1:1:1 stable complex that acts like soluble gp42 in B-cell fusion but not in epithelial cell fusion.

    abstract::Epstein-Barr virus (EBV) is a herpesvirus that infects cells by fusing its lipid envelope with the target cell membrane. The fusion process requires the actions of viral glycoproteins gH, gL, and gB for entry into epithelial cells and additionally requires gp42 for entry into B cells. To further study the roles of the...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00572-06

    authors: Kirschner AN,Omerovic J,Popov B,Longnecker R,Jardetzky TS

    更新日期:2006-10-01 00:00:00

  • Prolonged Antiretroviral Therapy Preserves HIV-1-Specific CD8 T Cells with Stem Cell-Like Properties.

    abstract:UNLABELLED:HIV-1-specific CD8 T cells can influence HIV-1 disease progression during untreated HIV-1 infection, but the functional and phenotypic properties of HIV-1-specific CD8 T cells in individuals treated with suppressive antiretroviral therapy remain less well understood. Here we show that a subgroup of HIV-1-spe...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00789-15

    authors: Vigano S,Negron J,Ouyang Z,Rosenberg ES,Walker BD,Lichterfeld M,Yu XG

    更新日期:2015-08-01 00:00:00

  • Locations of herpes simplex virus type 2 glycoprotein B epitopes recognized by human serum immunoglobulin G antibodies.

    abstract::Herpes simplex virus type 2 (HSV-2) glycoprotein B (gB-2) gene segments were expressed as recombinant proteins in Escherichia coli. gB-2 recombinant proteins were reacted with human serum immunoglobulin G (IgG) antibodies in Western immunoblot assays. Initially, samples were tested for the presence of HSV-1-specific a...

    journal_title:Journal of virology

    pub_type: 临床试验,杂志文章

    doi:10.1128/JVI.70.5.2950-2956.1996

    authors: Goade DE,Bell R,Yamada T,Mertz GJ,Jenison S

    更新日期:1996-05-01 00:00:00

  • Herpes simplex virus 1 recombinants with noninverting genomes frozen in different isomeric arrangements are capable of independent replication.

    abstract::Herpes simplex virus 1 genome consists of two covalently linked components, L and S, that invert relative to each other to yield four equimolar isomeric populations designated P (prototype), Is (inversion of S component), Il (inversion of L component), and Ils (inversion of L and S components) differing in the orienta...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.59.2.494-499.1986

    authors: Jenkins FJ,Roizman B

    更新日期:1986-08-01 00:00:00

  • Deep-Sequence Identification and Role in Virus Replication of a JC Virus Quasispecies in Patients with Progressive Multifocal Leukoencephalopathy.

    abstract::JC virus (JCV) is a DNA virus causing progressive multifocal leukoencephalopathy (PML) in immunodeficient patients. In the present study, 22 genetic quasispecies with more than 1.5% variant frequency were detected in JCV genomes from six clinical samples of PML by next-generation sequencing. A mutation from A to C at ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01335-16

    authors: Takahashi K,Sekizuka T,Fukumoto H,Nakamichi K,Suzuki T,Sato Y,Hasegawa H,Kuroda M,Katano H

    更新日期:2016-12-16 00:00:00

  • rgs-CaM Detects and Counteracts Viral RNA Silencing Suppressors in Plant Immune Priming.

    abstract::Primary infection of a plant with a pathogen that causes high accumulation of salicylic acid in the plant typically via a hypersensitive response confers enhanced resistance against secondary infection with a broad spectrum of pathogens, including viruses. This phenomenon is called systemic acquired resistance (SAR), ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00761-17

    authors: Jeon EJ,Tadamura K,Murakami T,Inaba JI,Kim BM,Sato M,Atsumi G,Kuchitsu K,Masuta C,Nakahara KS

    更新日期:2017-09-12 00:00:00

  • Role of RNA in enzymatic activity of the reverse transcriptase of hepatitis B viruses.

    abstract::The hepadnavirus reverse transcriptase is a multifunction enzyme. In addition to its role in DNA synthesis, the polymerase is required for RNA packaging and also functions as the primer for minus-strand DNA synthesis. Previously, we demonstrated that the protein-priming activity of the polymerase requires a viral RNA ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.12.8437-8442.1994

    authors: Wang GH,Zoulim F,Leber EH,Kitson J,Seeger C

    更新日期:1994-12-01 00:00:00

  • Within-Host Variations of Human Papillomavirus Reveal APOBEC Signature Mutagenesis in the Viral Genome.

    abstract::Persistent infection with oncogenic human papillomaviruses (HPVs) causes cervical cancer, accompanied by the accumulation of somatic mutations into the host genome. There are concomitant genetic changes in the HPV genome during viral infection; however, their relevance to cervical carcinogenesis is poorly understood. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00017-18

    authors: Hirose Y,Onuki M,Tenjimbayashi Y,Mori S,Ishii Y,Takeuchi T,Tasaka N,Satoh T,Morisada T,Iwata T,Miyamoto S,Matsumoto K,Sekizawa A,Kukimoto I

    更新日期:2018-05-29 00:00:00

  • The amino-terminal one-third of pseudorabies virus glycoprotein gIII contains a functional attachment domain, but this domain is not required for the efficient penetration of Vero cells.

    abstract::We have examined the attachment and penetration phenotypes of several glycoprotein gIII mutants of pseudorabies virus (PRV) and have identified the first one-third of gIII as a region that mediates efficient virus attachment to PK15 and Vero cells. This portion of gIII, amino acids 25 through 157 of the wild-type sequ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.67.5.2646-2654.1993

    authors: Flynn SJ,Burgett BL,Stein DS,Wilkinson KS,Ryan P

    更新日期:1993-05-01 00:00:00

  • Productive persistent infection of hematopoietic cells by human foamy virus.

    abstract::Human foamy virus can establish persistent infections in human hematopoietic cell lines, such as H92.1.7 (erythroblastoid cells), Jurkat (CD4+ T cells), and U937 (myeloid-monocytic cells). The infection is characterized by constant production of infectious viruses (for > 2 1/2 years) with no cytopathic effects on the ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.2.1250-1254.1996

    authors: Yu SF,Stone J,Linial ML

    更新日期:1996-02-01 00:00:00

  • Role of Substitutions in the Hemagglutinin in the Emergence of the 1968 Pandemic Influenza Virus.

    abstract::Hemagglutinin (HA) of H3N2/1968 pandemic influenza viruses differs from the putative avian precursor by seven amino acid substitutions. Substitutions Q226L and G228S are known to be essential for adaptation of avian HA to mammals. We found that introduction of avian-virus-like amino acids at five other HA positions (p...

    journal_title:Journal of virology

    pub_type: 历史文章,杂志文章

    doi:10.1128/JVI.01292-15

    authors: Van Poucke S,Doedt J,Baumann J,Qiu Y,Matrosovich T,Klenk HD,Van Reeth K,Matrosovich M

    更新日期:2015-12-01 00:00:00

  • The BFRF1 gene of Epstein-Barr virus encodes a novel protein.

    abstract::Computer analysis of the Epstein-Barr virus (EBV) genome indicates there are approximately 100 open reading frames (ORFs). Thus far about 30 EBV genes divided into the categories latent and lytic have been identified. The BamHI F region of EBV is abundantly transcribed during lytic replication. This region is highly c...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.7.3235-3244.2000

    authors: Farina A,Santarelli R,Gonnella R,Bei R,Muraro R,Cardinali G,Uccini S,Ragona G,Frati L,Faggioni A,Angeloni A

    更新日期:2000-04-01 00:00:00

  • Sequence relationships between adenovirus 2 early RNA and viral RNA size classes synthesized at 18 hours after infection.

    abstract::Synthesis of cytoplasmic viral RNA was studied during infection of cultured human (KB) cells with adenovirus 2. At 6 h, before viral DNA synthesis began 5% of the poly(A)-containing RNA hybridized to viral DNA; by 12 h and at later times more than 80% was virus specified. At 18 h after infection, four major size class...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.15.1.137-144.1975

    authors: Tal J,Craig EA,Raskas HJ

    更新日期:1975-01-01 00:00:00

  • Synergistic roles of antibody and interferon in noncytolytic clearance of Sindbis virus from different regions of the central nervous system.

    abstract::Sindbis virus (SINV) is an alphavirus that causes infection of neurons and encephalomyelitis in adult immunocompetent mice. Recovery can occur without apparent neurological damage. To better define the factors facilitating noncytolytic clearance of SINV in different regions of the central nervous system (CNS) and the ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01152-06

    authors: Burdeinick-Kerr R,Wind J,Griffin DE

    更新日期:2007-06-01 00:00:00

  • Isolation and characterization of herpes simplex virus type 1 host range mutants defective in viral DNA synthesis.

    abstract::Cell lines were generated by cotransfection of Vero cells with pSV2neo and a plasmid containing the herpes simplex virus type 1 (HSV-1) EcoRI D fragment (coordinates 0.086 to 0.194). One such cell line (S22) contained the genes for alkaline exonuclease and several uncharacterized functions. Three mutant isolates of HS...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.62.1.91-99.1988

    authors: Carmichael EP,Kosovsky MJ,Weller SK

    更新日期:1988-01-01 00:00:00

  • Human immunodeficiency virus type 1 pol gene mutations in an AIDS patient treated with multiple antiretroviral drugs.

    abstract::Multiple mutations were found in the human immunodeficiency virus pol gene following treatment of an AIDS patient with antiretroviral drugs. After approximately 2.5 years of monthly alternating therapy with 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC), most of the pol sequences amplified from the p...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.67.12.7271-7275.1993

    authors: Fitzgibbon JE,Farnham AE,Sperber SJ,Kim H,Dubin DT

    更新日期:1993-12-01 00:00:00

  • Multiple Pathways To Avoid Beta Interferon Sensitivity of HIV-1 by Mutations in Capsid.

    abstract::Type I interferons (IFNs), including alpha IFN (IFN-α) and IFN-β, potently suppress HIV-1 replication by upregulating IFN-stimulated genes (ISGs). The viral capsid protein (CA) partly determines the sensitivity of HIV-1 to IFNs. However, it remains to be determined whether CA-related functions, including utilization o...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00986-19

    authors: Sultana T,Mamede JI,Saito A,Ode H,Nohata K,Cohen R,Nakayama EE,Iwatani Y,Yamashita M,Hope TJ,Shioda T

    更新日期:2019-11-13 00:00:00

  • Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export.

    abstract::HIV-1's Rev protein forms a homo-oligomeric adaptor complex linking viral RNAs to the cellular CRM1/Ran-GTP nuclear export machinery through the activity of Rev's prototypical leucine-rich nuclear export signal (NES). In this study, we used a functional fluorescently tagged Rev fusion protein as a platform to study th...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02107-16

    authors: Behrens RT,Aligeti M,Pocock GM,Higgins CA,Sherer NM

    更新日期:2017-01-18 00:00:00

  • CXC chemokine ligand 10 controls viral infection in the central nervous system: evidence for a role in innate immune response through recruitment and activation of natural killer cells.

    abstract::How chemokines shape the immune response to viral infection of the central nervous system (CNS) has largely been considered within the context of recruitment and activation of antigen-specific lymphocytes. However, chemokines are expressed early following viral infection, suggesting an important role in coordinating i...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.78.2.585-594.2004

    authors: Trifilo MJ,Montalto-Morrison C,Stiles LN,Hurst KR,Hardison JL,Manning JE,Masters PS,Lane TE

    更新日期:2004-01-01 00:00:00

  • Restriction endonuclease from Haemophilus gallinarum (HgaI) cleaves polyoma DNA at four locations.

    abstract::A restriction endonuclease obtained from Haemophilus gallinarum (hgaI) cleaves polyoma DNA at four specific sites. Using the EcoRI, HindIII, and HpaII endonuclease restriction sites as reference, the four HgaI cleavage sites were mapped at 0.02, 0.14, 0.27, and 0.48 fractional lengths, clockwise, from the single EcoR...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.18.2.793-798.1976

    authors: Shishido K,Berg P

    更新日期:1976-05-01 00:00:00

  • Control of the papillomavirus early-to-late switch by differentially expressed SRp20.

    abstract::The viral early-to-late switch of papillomavirus infection is tightly linked to keratinocyte differentiation and is mediated in part by alternative mRNA splicing. Here, we report that SRp20, a cellular splicing factor, controls the early-to-late switch via interactions with A/C-rich RNA elements. An A/C-rich SE4 eleme...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01719-08

    authors: Jia R,Liu X,Tao M,Kruhlak M,Guo M,Meyers C,Baker CC,Zheng ZM

    更新日期:2009-01-01 00:00:00