Abstract:
:The group A rotaviruses are composed of at least seven serotypes. Serotype specificity is defined mainly by an outer capsid protein, VP7. In contrast, the other surface protein, VP3 (775 amino acids), appears to be associated with both serotype-specific and heterotypic immunity. To identify the cross-reactive and serotype-specific neutralization epitopes on VP3 of human rotavirus, we sequenced the VP3 gene of antigenic mutants resistant to each of seven anti-VP3 neutralizing monoclonal antibodies (N-MAbs) which exhibited heterotypic or serotype 2-specific reactivity, and we defined three distinct neutralization epitopes on VP3. The mutants sustained single amino acid substitutions at position 305, 392, 433, or 439. Amino acid position 305 was critical to epitope I, whereas amino acid position 433 was critical to epitope III. In contrast, epitope II appeared to be more dependent upon conformation and protein folding because both amino acid positions 392 and 439 appeared to be critical. These four positions clustered in a relatively limited area of VP5, the larger of the two cleavage products of VP3. At the positions where amino acid substitutions occurred, there was a correlation between amino acid sequence homology among different serotypes and the reactivity patterns of various viruses with the N-MAbs used for selection of mutants. A synthetic peptide (amino acids 296 to 313) which included the sequence of epitope I reacted with its corresponding N-MAb, suggesting that the region contains a sequential antigenic determinant. These data may prove useful in current efforts to develop vaccines against human rotavirus infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Taniguchi K,Maloy WL,Nishikawa K,Green KY,Hoshino Y,Urasawa S,Kapikian AZ,Chanock RM,Gorziglia Mdoi
10.1128/JVI.62.7.2421-2426.1988subject
Has Abstractpub_date
1988-07-01 00:00:00pages
2421-6issue
7eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::To determine mechanisms by which persistent viral infections are established and maintained, we initiated persistent infections of murine erythroleukemia (MEL) cells by using reovirus strains type 3 Abney and type 3 Dearing. Establishment of persistent reovirus infections of MEL cells was not associated with a signifi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.299-306.1997
更新日期:1997-01-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) infection results in the disruption of ND10 (also called nuclear bodies, PODs, or PML-associated bodies), which are nuclear matrix domains of unknown function present in mammalian cells. After ND10 disruption, viral transcription and DNA replication occur in globular nuclear domains...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10100-10107.1998
更新日期:1998-12-01 00:00:00
abstract::During studies of APOBEC3 (A3) anti-human immunodeficiency virus type 1 (anti-HIV-1) mechanisms, we identified a single cysteine at position 320 (C320) that disrupts A3DE activity. This residue is located in the recently identified DNA binding domain in A3G. Replacing C320 with a corresponding tyrosine from A3F (Y307)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00243-11
更新日期:2011-06-01 00:00:00
abstract::Affinity-purified medium T antigen encoded by NG59, a nontransforming mutant of polyomavirus, is specifically associated with a protein of 72,000 daltons (72K protein). Medium T antigens of wild-type polyomavirus and the transformation-competent mutant dl8 are not associated with the 72K protein. Instead, they form a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.405-410.1987
更新日期:1987-02-01 00:00:00
abstract::Flavopiridol, roscovitine, and other inhibitors of Cyclin-Dependent Kinases (CDK) inhibit the replication of a variety of viruses in vitro while proving nontoxic in human clinical trials of their effects against cancer. Consequently, these and other Pharmacological CDK inhibitors (PCIs) have been proposed as potential...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9352-9365.2004
更新日期:2004-09-01 00:00:00
abstract:UNLABELLED:In contrast to many viruses, human cytomegalovirus (HCMV) is unable to productively infect most cancer-derived cell lines. The mechanisms of this restriction are unclear. To explore this issue, we tested whether defined oncogenic alleles, including the simian virus 40 (SV40) T antigen (TAg) and oncogenic H-R...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00179-16
更新日期:2016-04-14 00:00:00
abstract::Adenoviral RNA sequences synthesized in nuclei isolated during the late phase of productive infection comprise, besides virus-associated, VA, RNA, five major species, ranging in size from approximately 13S to 55S. The latter RNA species is of the length predicted if the major transcriptional unit expressed during the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.2.394-403.1979
更新日期:1979-11-01 00:00:00
abstract::How chemokines shape the immune response to viral infection of the central nervous system (CNS) has largely been considered within the context of recruitment and activation of antigen-specific lymphocytes. However, chemokines are expressed early following viral infection, suggesting an important role in coordinating i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.585-594.2004
更新日期:2004-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02498-08
更新日期:2009-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.2936-2945.1991
更新日期:1991-06-01 00:00:00
abstract::Co-infection of neonatal BALB/c mice with Friend virus (FV) complex (containing defective spleen focus-forming virus [SFFV] and endogenous N-tropic leukemia-inducing helper virus [LLV-F]) and B-tropic Tennant leukemia virus (TenLV) resulted in the inhibition of LLV-F by the Fv-1(b) gene and recovery of a TenLV pseudot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.523-533.1973
更新日期:1973-09-01 00:00:00
abstract::ISG15, a 15-kDa interferon-induced protein that participates in antiviral defenses of mammals, is highly conserved among vertebrates. In fish, as in mammals, viral infection and interferon treatment induce isg15 expression. The two ubiquitin-like domains of ISG15 and the presence of a consensus LRLRGG sequence in the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01294-12
更新日期:2013-09-01 00:00:00
abstract:UNLABELLED:The measles virus (MeV) membrane fusion apparatus consists of a fusion protein trimer and an attachment protein tetramer. To trigger membrane fusion, the heads of the MeV attachment protein, hemagglutinin (H), bind cellular receptors while the 96-residue-long H stalk transmits the triggering signal. Structur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02846-13
更新日期:2014-06-01 00:00:00
abstract::A restriction endonuclease obtained from Haemophilus gallinarum (hgaI) cleaves polyoma DNA at four specific sites. Using the EcoRI, HindIII, and HpaII endonuclease restriction sites as reference, the four HgaI cleavage sites were mapped at 0.02, 0.14, 0.27, and 0.48 fractional lengths, clockwise, from the single EcoR...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.793-798.1976
更新日期:1976-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00958-12
更新日期:2012-09-01 00:00:00
abstract::IgG1 b12 is a broadly neutralizing antibody against human immunodeficiency virus type 1 (HIV-1). The epitope recognized by b12 overlaps the CD4 receptor-binding site (CD4bs) on gp120 and has been a target for vaccine design. Determination of the three-dimensional structure of immunoglobulin G1 (IgG1) b12 allowed model...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5863-5876.2003
更新日期:2003-05-01 00:00:00
abstract::We prepared retrovirus packaging cell lines containing gag-pol genes from spleen necrosis virus (expressed from a cytomegalovirus promoter and the simian virus 40 (SV40) polyadenylation sequences) and, on a separate vector, either the env gene from spleen necrosis virus (expressed from the Rous sarcoma virus promoter ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3209-3212.1989
更新日期:1989-07-01 00:00:00
abstract::Herpes simplex virus (HSV)-infected cells can activate the human complement system without interference of specific anti-HSV antibodies. Analysis by flow cytometry showed that C3-like molecules were deposited on the membrane of the infected cell when incubated with human serum without specific antibodies. Depletion of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.847-850.1988
更新日期:1988-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00138-09
更新日期:2009-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4351-4360.2000
更新日期:2000-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01714-13
更新日期:2014-01-01 00:00:00
abstract::A 4,500-bp BamHI fragment, located within the HindIII A segment of the vaccinia virus genome, was found to contain eight potential coding regions for polypeptides of 78 to 346 amino acids. The open reading frames with 133, 346, and 125 codons were homologous to profilin (an actin-binding protein), 3-beta-hydroxysteroi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.4598-4608.1991
更新日期:1991-09-01 00:00:00
abstract::Members of the Geminiviridae have single-stranded DNA genomes that replicate in nuclei of infected plant cells. All geminiviruses encode a conserved protein (Rep) that catalyzes initiation of rolling-circle replication. Earlier studies showed that three conserved motifs-motifs I, II, and III-in the N termini of gemini...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02143-10
更新日期:2011-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.312-316.1985
更新日期:1985-10-01 00:00:00
abstract::The Graffi murine leukemia virus (MuLV) is a retroviral mixture that induces predominantly myeloid leukemia in several inbred strains of mice. To analyze the viral component responsible for the myeloid leukemogenesis, we cloned several proviruses from a Graffi MuLV-infected cell line. Several infectious molecular clon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4722-4731.1993
更新日期:1993-08-01 00:00:00
abstract::When human adenovirus type 2 or 12 infects cells, either productively or non-productively, three classes of viral deoxyribonucleic acid (DNA) are found within the cells: (i) viral DNA which cosediments with DNA extracted from infectious adenovirions at 31.3S for adenovirus type 2 and at 29.0S for adenovirus type 12, (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.707-719.1971
更新日期:1971-06-01 00:00:00
abstract::A number of cDNA clones complementary to measles virus mRNA and 50S genome RNA have been generated. These clones have been mapped by restriction enzyme analysis and were subsequently sequenced by the method of Maxam and Gilbert (A. M. Maxam and W. Gilbert, Methods Enzymol. 65:499-560, 1980). Computer analysis of these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.186-193.1985
更新日期:1985-04-01 00:00:00
abstract::A tetracycline-regulated gene expression system and a panel of novel monoclonal antibodies were used to examine the subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus (HCV) NS3-NS4A complex in inducible cell lines. The NS3 serine protease domain and the full-length NS3 protein ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2293-2304.2000
更新日期:2000-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5385-5387.2000
更新日期:2000-06-01 00:00:00
abstract::The rapid and accurate identification of pathogens is critical in the control of infectious disease. To this end, we analyzed the capacity for viral detection and identification of a newly described high-density resequencing microarray (RMA), termed PathogenID, which was designed for multiple pathogen detection using ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00771-10
更新日期:2010-09-01 00:00:00