Abstract:
:We previously reported that exosomal transfer of hepatitis C virus (HCV) positive-strand RNA from human Huh-7 hepatoma cells to human plasmacytoid dendritic cells (pDCs) triggers pDC alpha/beta interferon (IFN-α/β) production in a Toll-like receptor 7 (TLR7)-dependent, virus-independent manner. Here we show that human pDCs are also activated by a TLR7-dependent, virus-independent, exosomal RNA transfer mechanism by human and mouse hepatoma and nonhepatoma cells that replicate the negative-strand lymphocytic choriomeningitis virus (LCMV).
journal_name
J Viroljournal_title
Journal of virologyauthors
Wieland SF,Takahashi K,Boyd B,Whitten-Bauer C,Ngo N,de la Torre JC,Chisari FVdoi
10.1128/JVI.01714-13subject
Has Abstractpub_date
2014-01-01 00:00:00pages
752-7issue
1eissn
0022-538Xissn
1098-5514pii
JVI.01714-13journal_volume
88pub_type
杂志文章abstract:UNLABELLED:Human metapneumovirus (HMPV), a recently discovered paramyxovirus, infects nearly 100% of the world population and causes severe respiratory disease in infants, the elderly, and immunocompromised patients. We previously showed that HMPV binds heparan sulfate proteoglycans (HSPGs) and that HMPV binding requir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01362-16
更新日期:2016-09-29 00:00:00
abstract::Hemagglutinin (HA) of H3N2/1968 pandemic influenza viruses differs from the putative avian precursor by seven amino acid substitutions. Substitutions Q226L and G228S are known to be essential for adaptation of avian HA to mammals. We found that introduction of avian-virus-like amino acids at five other HA positions (p...
journal_title:Journal of virology
pub_type: 历史文章,杂志文章
doi:10.1128/JVI.01292-15
更新日期:2015-12-01 00:00:00
abstract::Many viruses induce type I interferon responses by activating cytoplasmic RNA sensors, including the RIG-I-like receptors (RLRs). Although two members of the RLR family, RIG-I and MDA5, have been implicated in host control of virus infection, the relative role of each RLR in restricting pathogenesis in vivo remains un...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01403-13
更新日期:2013-11-01 00:00:00
abstract::Disruption of Epstein-Barr virus (EBV) latency is mediated by ZEBRA, the protein product of the immediate-early EBV gene, BZLF1. In vitro, phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC), induces reactivation of EBV. However, the physiological stimuli responsible for the disruption ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.5810-5818.2000
更新日期:2000-07-01 00:00:00
abstract::After herpes simplex virus infection of hamster kidney cells there is an induction of nucleoside phosphotransferase activity which can utilize AMP as phosphate donor. The activity is immunologically specific for the infected cell and is induced concomitantly with the virus-coded pyrimidine deoxynucleoside kinase activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.1056-1059.1976
更新日期:1976-03-01 00:00:00
abstract::All herpesviruses encode a homolog of glycoprotein M (gM), which appears to function in virion morphogenesis. Despite its conservation, gM is inessential for the lytic replication of alphaherpesviruses. In order to address the importance of gM in gammaherpesviruses, we disrupted it in the murine gammaherpesvirus 68 (M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3459-3467.2005
更新日期:2005-03-01 00:00:00
abstract::Mechanisms by which the plus-sense RNA genomes of picornaviruses are replicated remain poorly defined, but existing models do not suggest a role for sequences encoding the capsid proteins. However, candidate RNA replicons (delta P1 beta gal and delta P1Luc), representing the sequence of human rhinovirus 14 virus (HRV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1941-1952.1996
更新日期:1996-03-01 00:00:00
abstract::Astrovirus is one of the major causes of infant and childhood diarrhea worldwide. Our understanding of astrovirus pathogenesis trails behind our knowledge of its molecular and epidemiologic properties. Using a recently developed small-animal model, we investigated the mechanisms by which astrovirus induces diarrhea an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1564-1574.2004
更新日期:2004-02-01 00:00:00
abstract::Four mutants of the virulent Mahoney strain of poliovirus were generated by introducing mutations in nucleotides (nt) 128 to 134 of the genome, a region that contains a part of the stem-loop II (SLII) structure located within the internal ribosomal entry site (IRES; nt 120 to 590) (K. Shiroki, T. Ishii, T. Aoki, Y. Ot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2398-2405.1998
更新日期:1998-03-01 00:00:00
abstract::Dendritic cells (DC) are considered to be important contributors to human immunodeficiency virus type 1 (HIV-1) transmission and pathogenesis. As the first target cells in mucosal tissues, they can be become productively infected and can also capture virions and transfer them efficiently to CD4(+) T cells located with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01420-08
更新日期:2009-03-01 00:00:00
abstract::Enhancins are metalloproteinases, first identified in granuloviruses, that can enhance nucleopolyhedrovirus (NPV) potency. We had previously identified two enhancin genes (E1 and E2) in the Lymantria dispar multinucleocapsid NPV (LdMNPV) and showed that both were functional. For this study, we have extended our analys...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.16.10578-10588.2005
更新日期:2005-08-01 00:00:00
abstract::A limited period of chemotherapy during primary immunodeficiency virus infection might provide a long-term clinical benefit even if treatment is initiated at a time point when virus is already detectable in plasma. To evaluate this strategy, we infected rhesus macaques with the pathogenic simian/human immunodeficiency...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1704-1711.2000
更新日期:2000-02-01 00:00:00
abstract::The adenovirus major late promoter (MLP) is active during both the early and late phases of infection. During the early phase the activity of the MLP is similar to those of the other early viral promoters, but during the late phase the rate of transcription from the MLP becomes much greater by comparison. We report he...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.51-60.1990
更新日期:1990-01-01 00:00:00
abstract::Reovirus encephalitis in mice was used as a model system to investigate astrocyte activation (astrogliosis) following viral infection of the brain. Reovirus infection resulted in astrogliosis, as evidenced by increased expression of glial fibrillary acidic protein (GFAP), and the upregulation of genes that have been p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02027-18
更新日期:2019-05-01 00:00:00
abstract::Recent studies have proposed that adeno-associated viruses (AAVs) are not evolutionarily linked to other mammalian autonomous parvoviruses but are more closely linked to the autonomous parvoviruses of birds. To better understand the relationship between primate and avian AAVs (AAAVs), we cloned and sequenced the genom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.12.6799-6810.2003
更新日期:2003-06-01 00:00:00
abstract::Influenza A virus (IAV) is a highly transmissible respiratory pathogen and a major cause of morbidity and mortality around the world. Nucleoprotein (NP) is an abundant IAV protein essential for multiple steps of the viral life cycle. Our recent proteomic study of the IAV-host interaction network found that TRIM41 (tri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00905-18
更新日期:2018-07-31 00:00:00
abstract::The processes that facilitate transport of integral membrane proteins though the secretory pathway and subsequently target them to particular cellular membranes are relevant to almost every field of biology. These transport processes involve integration of proteins into the membrane of the endoplasmic reticulum (ER), ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.19.12528-12535.2005
更新日期:2005-10-01 00:00:00
abstract::By using in situ hybridization, we localized three endogenous chicken retrovirus loci associated with the gs- chf- phenotype to distinct regions on chromosome 1. These three loci, ev4, ev5, and ev8, along with the two other known gs- chf--associated loci in White Leghorn chickens, ev1 and ev13, appear to be evenly dis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.2.737-740.1982
更新日期:1982-08-01 00:00:00
abstract::Replication-defective vaccine vectors based on vesicular stomatitis virus (VSV) lacking its envelope glycoprotein gene (G) are highly effective in animal models. However, such ΔG vectors are difficult to grow because they require complementation with the VSV G protein. In addition, the complementing G protein induces ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01852-10
更新日期:2011-03-01 00:00:00
abstract::The smallest capsid proteins (SCPs) of the human herpesviruses differ substantially in size and sequence and are thought to impart some unique aspects of infection to their respective viruses. We used electron cryomicroscopy and antibody labeling to show that the 8-kDa SCP of human cytomegalovirus is attached only to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.1327-1332.2005
更新日期:2005-01-01 00:00:00
abstract::The matrix (M1) protein of influenza virus is a major structural component, involved in regulation of viral ribonucleoprotein transport into and out of the nucleus. Early in infection, M1 is distributed in the nucleus, whereas later, it is localized predominantly in the cytoplasm. Using immunofluorescence microscopy a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.439-445.1995
更新日期:1995-01-01 00:00:00
abstract::Carcinogenic progression of a human papillomavirus (HPV)-infected cell is often associated with integration of the viral genome in a manner which results in the loss of expression of the viral regulatory protein E2. One function of E2 is the regulation of expression of the viral oncogenes, E6 and E7. Introduction of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7791-7799.1995
更新日期:1995-12-01 00:00:00
abstract::Adenovirus types 5 and 3 (Ad5 and Ad3), two human adenovirus serotypes of evolutionarily divergent subgroups, show very different levels of E1A gene expression early after infection of permissive cells. Since adenovirus E1A gene expression is known to be transcriptionally autoregulated, we have investigated the differ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1833-1838.1989
更新日期:1989-04-01 00:00:00
abstract::The human immunodeficiency virus rev gene product regulates the expression of viral structural genes. It was recently shown that Rev regulates the export of viral structural mRNAs from the nucleus to the cytoplasm. Analysis of Rev subcellular localization reveals marked accumulation in the nucleolus, suggesting a role...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.881-885.1990
更新日期:1990-02-01 00:00:00
abstract::Herpes simplex virus (HSV)-infected cells can activate the human complement system without interference of specific anti-HSV antibodies. Analysis by flow cytometry showed that C3-like molecules were deposited on the membrane of the infected cell when incubated with human serum without specific antibodies. Depletion of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.847-850.1988
更新日期:1988-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) in vivo is known to establish persistent infection in resting, circulating memory B cells and to productively replicate in plasma cells. Until now, the molecular mechanism of how EBV switches from latency to lytic replication in vivo was not known. Here, we report that the plasma cell differen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01055-07
更新日期:2007-12-01 00:00:00
abstract::We have studied the functions of the intracellular RNAs of mouse mammary tumor virus (MMTV) by purification and translation in vitro. Two major size classes of MMTV RNA, 35S and 24S RNA, were isolated from MMTV-infected rat (XC) cells and cultured mammary tumor cells by preparative hybridization of whole cell or polya...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.207-218.1981
更新日期:1981-07-01 00:00:00
abstract::Here we show that the adenovirus early region 4 (E4) open reading frame 4 (ORF4) protein autoregulates its own transcription by inhibiting adenovirus E1A-induced activation of E4 transcription both in transient transfection experiments and during lytic virus growth. The inhibitory activity of E4-ORF4 was selective for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3844-3851.1996
更新日期:1996-06-01 00:00:00
abstract::We have examined the influence of the V1/V2 region of the human immunodeficiency virus type 1 (HIV-1) gp120 on certain biologic properties of the virus. We observed that on the genomic background of the T-cell-line-tropic strain, HIV-1SF2mc, both the V1 and V2 domains of the macrophage-tropic strain, HIV-1SF162mc, in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2253-2259.1994
更新日期:1994-04-01 00:00:00
abstract::Corynebacteriophages beta and gamma are closely related but heteroimmune; hence, gamma reproduces in C7(beta). A series of gamma mutants, designated gamma-bin (beta-inhibited), has been isolated. They reproduce in only 2 to 14% of infected C7(beta) cells, and, as a result, plaque with an efficiency of 10(-4) to 10(-5)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.28-33.1978
更新日期:1978-10-01 00:00:00