Abstract:
:Replication-defective vaccine vectors based on vesicular stomatitis virus (VSV) lacking its envelope glycoprotein gene (G) are highly effective in animal models. However, such ΔG vectors are difficult to grow because they require complementation with the VSV G protein. In addition, the complementing G protein induces neutralizing antibodies in animals and thus limits multiple vector applications. In the process of generating an experimental Nipah virus (a paramyxovirus) vaccine, we generated two defective VSVΔG vectors, each expressing one of the two Nipah virus (NiV) glycoproteins (G and F) that are both required for virus entry to host cells. These replication-defective VSV vectors were effective at generating NiV neutralizing antibody in mice. Most interestingly, we found that these two defective viruses could be grown together and passaged in tissue culture cells in the absence of VSV G complementation. This mixture of complementing defective viruses was also highly effective at generating NiV neutralizing antibody in animals. This novel approach to growing and producing a vaccine from two defective viruses could be generally applicable to vaccine production for other paramyxoviruses or for other viruses where the expression of at least two different proteins is required for viral entry. Such an approach minimizes biosafety concerns that could apply to single, replication-competent VSV recombinants expressing all proteins required for infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chattopadhyay A,Rose JKdoi
10.1128/JVI.01852-10subject
Has Abstractpub_date
2011-03-01 00:00:00pages
2004-11issue
5eissn
0022-538Xissn
1098-5514pii
JVI.01852-10journal_volume
85pub_type
杂志文章abstract::Polyoma virus complementary RNA, synthesized in vitro by using highly purified Escherichia coli RNA polymerase and nondefective form I polyoma DNA, was translated in a wheat germ cell-free system. Polypeptides were synthesized that comigrated on sodium dodecyl sulfate-polyacrylamide gels with the polyoma capsid protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.2.570-578.1978
更新日期:1978-02-01 00:00:00
abstract::A protamine-like protein named P6.9 is thought to play a role in the condensation of genomes of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) during an infection. Previous studies have shown that P6.9 is phosphorylated immediately upon synthesis and dephosphorylated upon the entry of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00438-12
更新日期:2012-11-01 00:00:00
abstract::The ability of certain vesicular stomatitis virus (VSV; Indiana serotype) temperature-sensitive (ts) mutants to synthesize intracellular viral complementary RNA (vcRNA) at permissive or nonpermissive temperatures for productive infections has been investigated. Mutants belonging to complementation groups II, III, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.1.157-169.1976
更新日期:1976-10-01 00:00:00
abstract::Hepatitis delta virus (HDV) contains a circular RNA genome of 1.7 kb. HDV RNA replication is thought to proceed via a rolling-circle mechanism that is dependent on autocatalytic cleavage and ligation reactions. However, it has never been established that these ribozyme activities are indeed involved in HDV RNA replica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2228-2234.1993
更新日期:1993-04-01 00:00:00
abstract::We generated Chinese hamster ovary cell lines that stably express wild-type, secreted, and glycosylphosphatidylinositol (GPI)-anchored envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1). The cells expressing wild-type Env (WT cells) express both the precursor gp160 and the mature gp120/gp41 and readi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7060-7066.1993
更新日期:1993-12-01 00:00:00
abstract::Recombinant Epstein-Barr viruses (EBV) with a translation termination codon mutation inserted into the nuclear protein 3A (EBNA-3A) or 3C (EBNA-3C) open reading frame were generated by second-site homologous recombination. These mutant viruses were used to infect primary B lymphocytes to assess the requirement of EBNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2014-2025.1993
更新日期:1993-04-01 00:00:00
abstract::Polyomaviruses are small nonenveloped particles with a circular double-stranded genome, approximately 5 kbp in size. The mammalian polyomaviruses mainly cause persistent subclinical infections in their natural nonimmunocompromised hosts. In contrast, the polyomaviruses of birds--avian polyomavirus (APV) and goose hemo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3523-3531.2006
更新日期:2006-04-01 00:00:00
abstract::Breast cancer is the second leading cause of cancer-related deaths in women in the United States. Triple-negative breast cancer constitutes a subset of breast cancer that is associated with higher rates of relapse, decreased survival, and limited therapeutic options for patients afflicted with this type of breast canc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01411-19
更新日期:2019-11-13 00:00:00
abstract::The ability of human immunodeficiency virus type 1 (HIV-1) to transduce nondividing cells is key to infecting terminally differentiated macrophages, which can serve as a long-term reservoir of HIV-1 infection. The mutation N57A in the viral CA protein renders HIV-1 cell cycle dependent, allowing examination of HIV-1 i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00214-19
更新日期:2019-04-17 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) ICP34.5 gene is a neurovirulence gene in mice. In addition, some ICP34.5 mutants have been reported to have a reduced efficiency of induced reactivation as measured by in vitro explantation of latently infected mouse ganglia. However, since spontaneous reactivation is almost non...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.3033-3041.1995
更新日期:1995-05-01 00:00:00
abstract::Two major DNA variants have been isolated from serially propagated stocks of the WR strain of vaccinia virus. Restriction enzyme mapping of the two variants with HindIII, AvaI, XhoI, SstII, and SmaI revealed a 6.3-megadalton deletion in the smaller DNA variant. The deletion was mapped at ca. 6.8 megadaltons in from th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.1000-1010.1981
更新日期:1981-03-01 00:00:00
abstract::A human lymphoid cell line (F172-D8) excreting a human immunodeficiency virus type 1 (HIV-1) anti-gp41 monoclonal antibody was used to construct a plasmid containing the cDNA of the single-chain variable fragment (scFvD8) corresponding to this antibody. A stable human osteosarcoma cell line was obtained which expresse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5712-5715.2000
更新日期:2000-06-01 00:00:00
abstract::Antiviral innate immune responses can be triggered by accumulation of intracellular nucleic acids resulting from virus infections. Double-stranded RNA (dsRNA) can be detected by the cytoplasmic RNA helicase proteins RIG-I and MDA5, two proteins that share sequence similarities within a caspase recruitment domain (CARD...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01325-06
更新日期:2006-12-01 00:00:00
abstract::The limited efficiency of in vivo gene transfer by replication-deficient retroviral vectors remains an obstacle to achieving effective gene therapy for solid tumors. One approach to circumvent this problem is the use of replication-competent retroviral vectors. However, the application of such vectors is at a comparat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02470-06
更新日期:2007-07-01 00:00:00
abstract::The protein product of the v-myb oncogene of avian myeloblastosis virus, p48v-myb, differs structurally in several ways from its normal cellular homolog, p75c-myb. We demonstrated that the 11 specific amino acid substitutions found in two independent molecular clones of this virus were not required for the transformat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.1093-1096.1988
更新日期:1988-03-01 00:00:00
abstract::Gene transfer to differentiated airway epithelia with existing viral vectors is very inefficient when they are applied to the apical surface. This largely reflects the polarized distribution of receptors on the basolateral surface. To identify new receptor-ligand interactions that might be used to redirect vectors to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9234-9239.2000
更新日期:2000-10-01 00:00:00
abstract:UNLABELLED:The first discovered and sequenced hepatitis C virus (HCV) genome and the first in vivo infectious HCV clones originated from the HCV prototype strains HCV-1 and H77, respectively, both widely used in research of this important human pathogen. In the present study, we developed efficient infectious cell cult...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02877-14
更新日期:2015-01-01 00:00:00
abstract::Rinderpest virus is a morbillivirus and is the causative agent of a widespread and important disease of cattle. The viral genome is a single strand of RNA in the negative sense. We have constructed plasmids containing cDNA copies of the 5' and 3' termini of the virus separated by a reporter gene and have shown that an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1265-1271.1997
更新日期:1997-02-01 00:00:00
abstract:UNLABELLED:Little is known about the antiviral response in mollusks. As in other invertebrates, the interferon signaling pathways have not been identified, and in fact, there is a debate about whether invertebrates possess antiviral immunity similar to that of vertebrates. In marine bivalves, due to their filtering act...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00591-16
更新日期:2016-08-12 00:00:00
abstract::Human cytomegalovirus (HCMV) has been implicated as a potential cofactor in human immunodeficiency virus type 1 (HIV-1)-related disease. Previously, we reported that HCMV inhibits HIV-1 RNA and protein synthesis in cells productively infected with both viruses but, in transient assays, activates an HIV-1 long terminal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1645-1651.1995
更新日期:1995-03-01 00:00:00
abstract::The infected cell polypeptide 4 (ICP4) of herpes simplex virus 1 (HSV-1) is a regulator of viral transcription that is required for productive infection. Since viral genes are transcribed by cellular RNA polymerase II (RNA pol II), ICP4 must interact with components of the pol II machinery to regulate viral gene expre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00385-11
更新日期:2011-06-01 00:00:00
abstract::In the influenza H5N1 virus incident in Hong Kong in 1997, viruses that are closely related to H5N1 viruses initially isolated in a severe outbreak of avian influenza in chickens were isolated from humans, signaling the possibility of an incipient pandemic. However, it was not possible to prepare a vaccine against the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8303-8307.1999
更新日期:1999-10-01 00:00:00
abstract:UNLABELLED:Hepatitis C virus (HCV) requires proteins from the NS3-NS5B polyprotein to create a replicase unit for replication of its genome. The replicase proteins form membranous compartments in cells to facilitate replication, but little is known about their functional organization within these structures. We recentl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01931-15
更新日期:2015-12-30 00:00:00
abstract::Interspecies transmission of the transmissible spongiform encephalopathies (TSEs), or prion diseases, can result in the adaptation and selection of TSE strains with an expanded host range and increased virulence such as in the case of bovine spongiform encephalopathy and variant Creutzfeldt-Jakob disease. To investiga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5542-5547.2000
更新日期:2000-06-01 00:00:00
abstract::Early stages of infection by the mouse polyomavirus have been studied using HeLa cells stably expressing small interfering RNA to protein disulfide isomerase (PDI). Infectibility measured by nuclear T antigen expression was reduced commensurately with the degree of PDI downregulation. Infectibility was restored by tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01117-06
更新日期:2006-11-01 00:00:00
abstract::Productively infected bovine fibropapillomas were examined for bovine papillomavirus type 1 (BPV-1) E7 localization. BPV-1 E7 was observed in the cytoplasm of basal and lower spinous epithelial cells, coexpressed in the cytoplasm of basal cells with the E5 oncoprotein. E7 was also observed in nucleoli throughout the b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.513-521.2001
更新日期:2001-01-01 00:00:00
abstract::The promoter of the murine thymidine kinase gene contains a binding site for transcription factor E2F. Using cell lines (3T3-LT) conditionally expressing polyomavirus large T antigen from a hormone-responsive promoter and reporter gene constructs carrying the thymidine kinase promoter with intact or mutated E2F sites,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.1765-1771.1993
更新日期:1993-04-01 00:00:00
abstract::The temporal regulation of DNA replication is thought to be important for chromosome organization and genome stability. We show here that Epstein-Barr virus (EBV) genomes replicate in mid- to late S phase and that agents that accelerate replication timing of EBV reduce viral genome stability. Hydroxyurea (HU) treatmen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02115-08
更新日期:2009-03-01 00:00:00
abstract::The restrictive properties of tripartite motif-containing 5 alpha (TRIM5α) from small ruminant species have not been explored. Here, we identify highly similar TRIM5α sequences in sheep and goats. Cells transduced with ovine TRIM5α effectively restricted the lentivirus visna/maedi virus DNA synthesis. Proteasome inhib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00440-12
更新日期:2012-09-01 00:00:00
abstract::Recently, it was demonstrated that the coxsackievirus B3 variant PD (CVB3 PD) is able to infect coxsackievirus-adenovirus receptor (CAR)-lacking cells by using heparan sulfates (HS) as additional receptors (A. E. Zautner, U. Korner, A. Henke, C. Badorff, and M. Schmidtke, J. Virol. 77:10071-10077, 2003). For this stud...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01988-05
更新日期:2006-07-01 00:00:00