Abstract:
:To determine mechanisms by which persistent viral infections are established and maintained, we initiated persistent infections of murine erythroleukemia (MEL) cells by using reovirus strains type 3 Abney and type 3 Dearing. Establishment of persistent reovirus infections of MEL cells was not associated with a significant cytopathic effect despite the presence of high titers of infectious virus in the cultures (>10(5) PFU/ml of culture lysate). Maintenance of persistently infected MEL-cell cultures was associated with coevolution of mutant viruses and cells. Mutant viruses produced greater yields than the parental wild-type (wt) strains in MEL cells cured of persistent infection and in cells treated with ammonium chloride, a weak base that blocks viral disassembly. Mutant cells supported growth of wt infectious subvirion particles, which are disassembly intermediates generated in vitro by treatment of virions with chymotrypsin, substantially better than growth of wt virions. These findings indicate that viral and cellular mutations selected during maintenance of persistently infected MEL-cell cultures affect acid-dependent proteolysis of virions during entry into cells. We also found that wt infectious subvirion particles produce greater yields than wt virions in wt MEL cells, which suggests that inefficient viral disassembly in MEL cells favors establishment of persistent infection. Therefore, steps in reovirus replication leading to viral disassembly appear to be critical determinants of the capacity of MEL cells to support both establishment and maintenance of persistent reovirus infections.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wetzel JD,Chappell JD,Fogo AB,Dermody TSdoi
10.1128/JVI.71.1.299-306.1997subject
Has Abstractpub_date
1997-01-01 00:00:00pages
299-306issue
1eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::Six phages were isolated from sewage and from a lysogenic strain. Three of them, belonging to a new morphological group, had contractile tails and elongated heads with axial ratios of 2.4:1. Two phages, possessing short tails and very long heads with axial ratios of 3.5:1, were new isolates of an extremely rare group....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.706-711.1974
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abstract::Polyomaviruses are small nonenveloped particles with a circular double-stranded genome, approximately 5 kbp in size. The mammalian polyomaviruses mainly cause persistent subclinical infections in their natural nonimmunocompromised hosts. In contrast, the polyomaviruses of birds--avian polyomavirus (APV) and goose hemo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3523-3531.2006
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8908-8916.1996
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7599-7606.1999
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9039-9052.1999
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3534-3543.1993
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5522-5531.2002
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7541-7547.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.6.558-566.1968
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3145-3153.1994
更新日期:1994-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.2.362-367.1967
更新日期:1967-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.68.3.1987-1992.1994
更新日期:1994-03-01 00:00:00
abstract::Mosquito-borne alphaviruses, which replicate alternately and obligately in mosquitoes and vertebrates, appear to experience lower rates of evolution than do many RNA viruses that replicate solely in vertebrates. This genetic stability is hypothesized to result from the alternating host cycle, which constrains evolutio...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00