Abstract:
:Varicella-zoster virus (VZV) open reading frame 4 (ORF4) encodes a putative immediate-early protein which is homologous to herpes simplex virus type 1 (HSV-1) ICP27 on the basis of gene location and similarity in amino acid sequence. In transient expression assays, however, ORF4 and ICP27 exhibit different properties. ICP27 alone has little activity on target plasmids, but it acts as a transactivator or a transrepressor in the presence of other HSV-1 transactivators. In contrast, ORF4 directly transactivates plasmids containing homologous or heterologous promoters and has no apparent transrepressing activity. To further illuminate the functional similarities and differences between ORF4 and ICP27, Vero cell lines which express ORF4 under the inducible metallothionein promoter were constructed. Cell lines expressing functionally active ORF4 protein upregulated the expression of transfected VZV target plasmids but were unable to efficiently complement HSV-1 ICP27 mutants. These results indicate that, despite structural similarities, VZV ORF4 and HSV-1 ICP27 behave differently in transient expression assays and may play different roles in virus replication.
journal_name
J Viroljournal_title
Journal of virologyauthors
Moriuchi H,Moriuchi M,Smith HA,Cohen JIdoi
10.1128/JVI.68.3.1987-1992.1994subject
Has Abstractpub_date
1994-03-01 00:00:00pages
1987-92issue
3eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::Extracts from nodavirus-infected Drosophila cells contained detergent-labile 140S "young" particles much richer than mature virions in their content of protein alpha, a precursor of coat proteins beta and gamma. Incorporation studies in infected cells showed that most newly synthesized alpha protein was assembled into...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.9.3399-3406.1988
更新日期:1988-09-01 00:00:00
abstract::The presence of cycloheximide, an inhibitor of protein synthesis, during the period 30 to 60 min after vaccinia infection produced an irreversible block in virus replication. In contrast (i) cycloheximide given at earlier or later times, even for prolonged periods, did not prevent continuation of the infectious cycle ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.2.99-108.1970
更新日期:1970-02-01 00:00:00
abstract::The functions of delta antigens (HDAgs) in the morphogenesis of hepatitis delta virus (HDV) have been studied previously. The C terminus of large HDAg has been shown to complex with the small surface antigen (HBsAg) of helper hepatitis B virus, whereas the assembly of small HDAg requires interaction with the N terminu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2508-2514.1995
更新日期:1995-04-01 00:00:00
abstract::Viral infectivity factor (Vif) is one of the human immunodeficiency virus (HIV) accessory proteins and is conserved in the primate lentivirus group. This protein is essential for viral replication in vivo and for productive infection of nonpermissive cells, such as peripheral blood mononuclear cells (PBMC). Vif counte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.823-833.2005
更新日期:2005-01-01 00:00:00
abstract::The human immunodeficiency virus type 1 Tat protein binds to an RNA stem-loop structure called TAR which is present at the 5' end of all human immunodeficiency virus type 1 transcripts. This binding is centered on a bulge within the stem of TAR and is an essential step in the trans-activation process which results in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5196-5202.1991
更新日期:1991-10-01 00:00:00
abstract::To assess the possible role of cytotoxic T lymphocytes (CTLs) in containing the spread of human immunodeficiency virus in acutely infected individuals, the temporal evolution of the virus-specific CD8+ lymphocyte response was defined in simian immunodeficiency virus of macaques (SIVmac)-infected rhesus monkeys. A brie...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1707-1711.1993
更新日期:1993-03-01 00:00:00
abstract::The Kansas strain of bovine parainfluenza virus type 3 (BPIV3) is 100- to 1,000-fold restricted in replication in the respiratory tracts of nonhuman primates compared to human PIV3 (HPIV3), an important pathogen of infants and young children. BPIV3 is also restricted in replication in human infants and children, yet i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1141-1148.2003
更新日期:2003-01-01 00:00:00
abstract::West Nile virus (WNV) is a mosquito-borne Flavivirus that causes encephalitis in a subset of susceptible humans. Current treatment for WNV infections is supportive, and no specific therapy or vaccine is available. In this study, we directly tested the prophylactic and therapeutic efficacy of polyclonal antibodies agai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.12941-12949.2003
更新日期:2003-12-01 00:00:00
abstract::The terminal 500-base-pair alpha sequence of the herpes simplex virus 1 genome contains signals for cleavage (Pac1 and Pac2) of unit-length DNA molecules from concatemers in unique stretches of sequences designated Ub and Uc, respectively, and a cis site for cleavage designated DR1. We report that nuclear extracts fro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1059-1068.1989
更新日期:1989-03-01 00:00:00
abstract::Rabies virus nucleoprotein (N) plays vital roles in regulation of viral RNA transcription and replication by encapsidation of the nascent genomic RNA. Rabies virus N is phosphorylated, and previous studies demonstrated that mutation of the phosphorylated serine at position 389 to alanine resulted in reduction of viral...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4153-4161.2002
更新日期:2002-05-01 00:00:00
abstract::The feline homolog of the alpha-chemokine receptor CXCR4 has recently been shown to support cell-cell fusion mediated by CXCR4-dependent strains of human immunodeficiency virus (HIV) and strains of feline immunodeficiency virus (FIV) that have been selected for growth in the Crandell feline kidney (CrFK) cell line. In...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6475-6481.1998
更新日期:1998-08-01 00:00:00
abstract::The genome of varicella-zoster virus (VZV) is a linear, double-stranded molecule of DNA composed of a long (L) region covalently linked to a short (S) region. The S region is capable of inverting relative to a fixed orientation of the L region, giving rise to two equimolar populations. We have investigated other forms...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.194-200.1985
更新日期:1985-10-01 00:00:00
abstract::In search of an anti-transcriptase, antibody was raised in rabbits to partially purified, soluble NS protein present in cytoplasmic extracts of cells infected with the Indiana serotype of vesicular stomatitis (VSInd) virus. This antiserum gave specific reactions of identity by agar immunodiffusion with both cytoplasmi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.6.1357-1366.1975
更新日期:1975-06-01 00:00:00
abstract::The importance of neutralizing antibodies (NAbs) in protection against hepatitis C virus (HCV) remains controversial. We infused a chimpanzee with H06 immunoglobulin from a genotype 1a HCV-infected patient and challenged with genotype strains efficiently neutralized by H06 in vitro. Genotype 1a NAbs afforded no protec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01194-15
更新日期:2015-09-01 00:00:00
abstract::Relative to wild-type herpes simplex virus type 1 (HSV-1), ICP0-null mutant viruses reactivate inefficiently from explanted, latently infected mouse trigeminal ganglia (TG), indicating that ICP0 is not essential for reactivation but plays a central role in enhancing the efficiency of reactivation. The validity of thes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3240-3249.2001
更新日期:2001-04-01 00:00:00
abstract::Yaba tumor poxvirus deoxyribonucleic acid (DNA) has a density of 1.6905 in CsCl and its T(m) value in 0.015 m citrate in saline is 82.3. The guanine plus cytosine content estimated from these properties was taken to be 32.5 +/- 0.5%, a value 2 to 3% less than for DNA from other poxviruses reported to date. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.2.114-118.1969
更新日期:1969-02-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infects human cells by binding to surface CD4 molecules and directly fusing with the cell membrane. Although mouse cells expressing human CD4 bind HIV, they do not become infected, apparently because of a block in membrane fusion. To study this problem, we constructed a recombinant v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2149-2156.1990
更新日期:1990-05-01 00:00:00
abstract::HIV-1 infections cannot be completely eradicated by drug therapy, as the virus persists in reservoirs. Low-level plasma viremia has been detected in patients treated for over 7 years, but the cellular compartments that support this low-level viremia have not been identified. The decay of HIV-1 during treatment appears...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00556-13
更新日期:2013-07-01 00:00:00
abstract::Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has caused a pandemic of historic proportions and continues to spread globally, with enormous consequences to human health. Currently there is no vaccine, effective therapeutic, or prophylactic. As with other betacoronaviruses, attachment and entry o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01987-20
更新日期:2021-01-13 00:00:00
abstract:UNLABELLED:In vitro, infection of polarized human intestinal epithelial cells by coxsackievirus B3 (CVB3) depends on virus interaction with decay-accelerating factor (DAF), a receptor expressed on the apical cell surface. Although mice are highly susceptible to CVB3 infection when virus is delivered by intraperitoneal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03468-14
更新日期:2015-04-01 00:00:00
abstract::We have measured in 22 asymptomatic human immunodeficiency virus type 1-infected patients (10 rapid progressors and 12 slow progressors) the proviral load of CD4(+) T cells homogeneously superinfected by the same dose of a non-syncytium-inducing virus in the presence or in the absence of autologous CD8(+) T cells. We ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6648-6651.2000
更新日期:2000-07-01 00:00:00
abstract::Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a newly identified coronavirus (CoV), SARS-CoV. The spike (S) glycoprotein of CoV is the major structural protein responsible for induction of host immune response and virus neutralization by antibodies. Hence, knowledge of neutralization...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.6938-6945.2004
更新日期:2004-07-01 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV68) is a gammaherpesvirus that was first isolated from murid rodents. MHV68 establishes a latent infection in the spleen and other lymphoid organs. Several gammaherpesviruses, including herpesvirus saimiri, human herpesvirus 8, and MHV68, encode proteins with extensive homology to the D-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.7016-7023.2000
更新日期:2000-08-01 00:00:00
abstract::We describe spontaneous second-site reversions within the coat protein open reading frame that rescue the systemic-spread phenotype and increase virion stability of a mutant of brome mosaic virus. Based on the crystal structure of the related cowpea chlorotic mottle virus, we show that the modified residues are spatia...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.2500-2504.1997
更新日期:1997-03-01 00:00:00
abstract::Little is known about the mechanisms used by enveloped viruses to separate themselves from the cell surface at the final step of budding. However, small sequences in the Gag proteins of several retroviruses (L domains) have been implicated in this process. A sequence has been identified in the M proteins of rhabdoviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3359-3365.1999
更新日期:1999-04-01 00:00:00
abstract::A number of retrovirus-like sequences have been cloned from chimpanzee DNA which constitute the chimpanzee homologs of the endogenous colobus type C virus CPC-1. One of the clones contains a nearly complete viral genome, but others have sustained deletions of 1 to 2 kilobases in the polymerase gene. The pattern of rel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.3.914-924.1982
更新日期:1982-09-01 00:00:00
abstract::We constructed a matched set of plasmids to investigate the interactions of essential core sequences of the simian virus 40 replication origin with flanking regulatory sequences. Deletions of either T-antigen-binding region I or the 21-base-pair repeated promoter elements reduced replication to 50 to 70% of wild-type ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.138-144.1986
更新日期:1986-01-01 00:00:00
abstract::Human papillomavirus type 16 (HPV16) is the primary etiologic agent for cervical cancer. The infectious entry of HPV16 into cells occurs via a so-far poorly characterized clathrin- and caveolin-independent endocytic pathway, which involves tetraspanin proteins and actin. In this study, we investigated the specific rol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02906-12
更新日期:2013-03-01 00:00:00
abstract::A soluble polymerase-template complex prepared from poliovirus-infected cells was found to incorporate radioactive UTP into trichloroacetic acid-insoluble RNA linearly for 8 h in the presence of ATP and Mg2+. Radioactive CTP or GTP was not incorporated under identical conditions. Nearest-neighbor analysis of the in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.61-68.1977
更新日期:1977-01-01 00:00:00
abstract:UNLABELLED:An understanding of the antigen-specific B-cell response to the influenza virus hemagglutinin (HA) is critical to the development of universal influenza vaccines, but it has not been possible to examine these cells directly because HA binds to sialic acid (SA) on most cell types. Here, we use structure-based...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03422-13
更新日期:2014-04-01 00:00:00