Abstract:
:In search of an anti-transcriptase, antibody was raised in rabbits to partially purified, soluble NS protein present in cytoplasmic extracts of cells infected with the Indiana serotype of vesicular stomatitis (VSInd) virus. This antiserum gave specific reactions of identity by agar immunodiffusion with both cytoplasmic and virion NS protein. NS antiserum also preferentially precipitated NS 3-H-labeled protein from infected cytoplasmic extracts, whereas anti-whole VSInd virion serum also precipitated N 3-H-labeled protein from extracts both of infected cytoplasm and virion nucleocapsids. Transcriptase activity of VSInd cytoplasmic or virion-derived nucleocapsids was effectively inhibited by ribonuclease-free immunoglubulin prepared from homologous NSInd antiserum or from anti-whole vesicular stomatitis virus serum. Transcriptase activity of heterologous New Jersey serotype (VSNJ) nucleocapsids and virions was not appreciably affected by anti-NSInd or by anti-whole VSInd virion gamma globulin. Anti-NS gamma glubulin immediately switched off RNA synthesis by actively transcribing VSInd nucleocapsids, a finding which suggests that NS antibody inhibits RNA chain elongation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Imblum RL,Wagner RRdoi
10.1128/JVI.15.6.1357-1366.1975subject
Has Abstractpub_date
1975-06-01 00:00:00pages
1357-66issue
6eissn
0022-538Xissn
1098-5514journal_volume
15pub_type
杂志文章abstract::Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) open reading frame 50 (ORF50) encodes a viral transcriptional activator, which binds to the KSHV promoter and stimulates the transcription of viral early and late genes, thus activating the lytic cycle of KSHV. We report here that KSHV ORF50 binds to the cellular pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.4.1909-1917.2001
更新日期:2001-02-01 00:00:00
abstract::The retroviral Gag protein plays the central role in the assembly process and can form membrane-enclosed, virus-like particles in the absence of any other viral products. These particles are similar to authentic virions in density and size. Three small domains of the human immunodeficiency virus type 1 (HIV-1) Gag pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4667-4677.1998
更新日期:1998-06-01 00:00:00
abstract::We have reinvestigated the subcellular distribution of the duck hepatitis B virus (DHBV) core protein in infected duck hepatocytes and in transfected cells. By using indirect immunofluorescence, the protein was found to be localized not only in the cytoplasm, as described previously, but also within the cell nucleus, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.2157-2164.2003
更新日期:2003-02-01 00:00:00
abstract::Herpes simplex virus type 1 packages its DNA genome into a precursor capsid, referred to as the procapsid. Of the three capsid-associated DNA-packaging proteins, UL17, UL25, and UL6, only UL17 and UL6 appear to be components of the procapsid, with UL25 being added subsequently. To determine whether the association of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2118-2126.2006
更新日期:2006-03-01 00:00:00
abstract::Like almost all of the positive-strand RNA viruses, hepatitis C virus (HCV) induces host intracellular membrane modification to form the membrane-bound viral replication complex (RC), within which viral replicases amplify the viral RNA genome. Despite accumulated information about how HCV co-opts host factors for vira...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01140-16
更新日期:2016-10-14 00:00:00
abstract::Adenovirus type 7 (Ad7) early region 1 mRNA species transcribed in rat cell lines transformed by the HindIII-I . J fragment (the left 7.8% of the viral genome) and in human KB cells infected with Ad7 were mapped on the viral genome, using S1 nuclease gel and diazobenzyloxymethyl paper hybridization techniques. At the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.337-352.1980
更新日期:1980-11-01 00:00:00
abstract::Ribosomes are observed intimately associated with nucleocapsids of vesicular, stomatitis virus, especially those that line structures that are either cytoplasmic vesicles or invaginations of the plasma membrane. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.2.447-452.1975
更新日期:1975-08-01 00:00:00
abstract::Autographa californica nuclear polyhedrosis virus late and very late mRNAs are transcribed by an RNA polymerase consisting of four virus-encoded polypeptides: LEF-8, LEF-9, LEF-4, and p47. The 464-amino-acid LEF-4 subunit contains the signature motifs of GTP:RNA guanylyltransferases (capping enzymes). Here, we show th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10020-10028.1998
更新日期:1998-12-01 00:00:00
abstract::To assess the possible role of cytotoxic T lymphocytes (CTLs) in containing the spread of human immunodeficiency virus in acutely infected individuals, the temporal evolution of the virus-specific CD8+ lymphocyte response was defined in simian immunodeficiency virus of macaques (SIVmac)-infected rhesus monkeys. A brie...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1707-1711.1993
更新日期:1993-03-01 00:00:00
abstract::Prion diseases are progressive fatal neurodegenerative illnesses caused by the accumulation of transmissible abnormal prion protein (PrP). To find treatments for prion diseases, we searched for substances from natural resources that inhibit abnormal PrP formation in prion-infected cells. We found that high-molecular-w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01862-16
更新日期:2017-02-28 00:00:00
abstract::The development of immunization strategies to protect against ocular infection with herpes simplex virus 1 (HSV-1) must address the issue of the effects of the strategy on the establishment of latency in the trigeminal ganglia (TG). It is the reactivation of this latent virus that can cause recurrent disease and corne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00913-14
更新日期:2014-07-01 00:00:00
abstract::The inducible transcription factor NF-kappaB is commonly activated upon RNA virus infection and is a key player in the induction and regulation of the innate immune response. Borna disease virus (BDV) is a neurotropic negative-strand RNA virus, which replicates in the nucleus of the infected cell and causes a persiste...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6043-6051.2005
更新日期:2005-05-01 00:00:00
abstract::Hepatitis A virus (HAV) has previously been reported to bind to human red blood cells through interaction with glycophorin A. Residue K221 of VP1 and the surrounding VP3 residues are involved in such an interaction. This capsid region is specifically recognized by the monoclonal antibody H7C27. A monoclonal antibody-r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00707-12
更新日期:2012-08-01 00:00:00
abstract::Jaagsiekte sheep retrovirus (JSRV) is the causative agent of a transmissible lung cancer of sheep known as ovine pulmonary carcinoma. Recently, we have found that the expression of the JSRV envelope (Env) is sufficient to transform mouse NIH 3T3 cells in classical transformation assays. To further investigate the mech...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.22.11002-11009.2001
更新日期:2001-11-01 00:00:00
abstract::Sendai virus (SV) and human parainfluenza virus type 1 (hPIV1) have genomes consisting of nonsegmented negative-sense RNA in which the six genes are separated by well-conserved intergenic (IG) sequences and transcriptional start (S) and end signals. In hPIV1-infected cells, transcriptional termination at the M-F gene ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8244-8251.2002
更新日期:2002-08-01 00:00:00
abstract::We examined the migratory properties of the mouse serum protein responsible for specific neutralization of the endogenous mouse xenotropic type C virus and demonstrated that this neutralizing factor can be transferred from mouse lipoproteins to human and to nonreactive mouse lipoproteins. After acceptance of this acti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.2.365-371.1982
更新日期:1982-05-01 00:00:00
abstract::To clarify the role of core protein phosphorylation in pregenomic-RNA encapsidation of human and duck hepatitis B viruses (HBV and DHBV, respectively), we have examined the phosphorylation states of different forms of intracellular HBV core protein and the phenotypic effects of mutations in the phosphorylation sites o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4721-4728.2000
更新日期:2000-05-01 00:00:00
abstract:UNLABELLED:Nonhuman primates (NHPs) are a historically important source of zoonotic viruses and are a gold-standard model for research on many human pathogens. However, with the exception of simian immunodeficiency virus (SIV) (family Retroviridae), the blood-borne viruses harbored by these animals in the wild remain i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00573-16
更新日期:2016-07-11 00:00:00
abstract::The antibiotic tunicamycin, which blocks the synthesis of glycoproteins, inhibited the production of infectious herpes simplex virus. In the presence of this drug, [14C]glucosamine and [3H]mannose incorporation was reduced in infected cells, whereas total protein synthesis was not affected. Gel electrophoresis of [2-3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.1.142-153.1980
更新日期:1980-04-01 00:00:00
abstract::The prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a formidable battle horse for the study of viral immunology, as well as viral persistence and associated diseases. Investigations with LCMV have uncovered basic mechanisms by which viruses avoid elimination by the host adaptive immune response. In ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00555-06
更新日期:2006-09-01 00:00:00
abstract::Whole-body bioimaging was used to study dissemination of vaccinia virus (VACV) in normal and in immune deficient (nu(-)/nu(-)) mice protected from lethality by postchallenge administration of ST-246. Total fluxes were recorded in the liver, spleen, lungs, and nasal cavities of live mice after intranasal infection with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03426-12
更新日期:2013-05-01 00:00:00
abstract::The U(S)3 open reading frame of herpes simplex virus 1 (HSV-1) was reported to encode two mRNAs each directing the synthesis of the same protein. We report that the U(S)3 gene encodes two proteins. The predominant U(S)3 protein is made in wild-type HSV-1-infected cells. The truncated mRNA and a truncated protein desig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8470-8479.2005
更新日期:2005-07-01 00:00:00
abstract::Assembly and maturation of retroviral particles requires the aggregation and controlled proteolytic cleavage of polyprotein core precursors by a precursor-encoded protease (PR). Active, mature retroviral PR is a dimer, and the accumulation of precursors at sites of assembly may facilitate subunit interaction and subse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6165-6172.1991
更新日期:1991-11-01 00:00:00
abstract::The development of vaccines against bluetongue, a prevalent livestock disease, has been focused on surface antigens that induce strong neutralizing antibody responses. Because of their antigenic variability, these vaccines are usually serotype restricted. We now show that a single highly conserved nonstructural protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00938-18
更新日期:2018-07-31 00:00:00
abstract::GP64, the major envelope glycoprotein of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) budded virion, is important for host cell receptor binding and mediates low-pH-triggered membrane fusion during entry by endocytosis. In the current study, we examined the functional role of the AcMNPV GP64 tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02104-07
更新日期:2008-04-01 00:00:00
abstract::We located a region of interstrain size variability in a short segment in an area at the right-hand end of the long unique sequence of the varicella-zoster viral genome. Varicella-zoster virus strains isolated in a district of Japan were classified into three groups on the basis of the size of this segment. Sequence c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2916-2921.1988
更新日期:1988-08-01 00:00:00
abstract::A detailed kinetic and quantitative analysis of the early and late biosynthetic events undergone by the human immunodeficiency virus type 1 envelope protein expressed by a recombinant vaccinia virus was performed. Early folding events that occurred in the endoplasmic reticulum included disulfide bond formation (t1/2 a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.2047-2055.1991
更新日期:1991-04-01 00:00:00
abstract::Activation of host innate immune responses was studied in severe acute respiratory syndrome coronavirus (SCV)-infected human A549 lung epithelial cells, macrophages, and dendritic cells (DCs). In all cell types, SCV-specific subgenomic mRNAs were seen, whereas no expression of SCV proteins was found. No induction of c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13800-13805.2005
更新日期:2005-11-01 00:00:00
abstract::Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. In order to systematically investigate the role of the membrane-proximal residues in the M2 cytoplasmic tail in virus replication, we utilized scanning and directed alanine mutagenesis in combination wit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05970-11
更新日期:2011-12-01 00:00:00
abstract::A recent experiment involving simian immunodeficiency virus (SIV) infection of macaques revealed that the infectivity of this virus decreased over the first few months of infection. Based on this observation, we introduce a viral dynamic model in which viral infectivity varies over time. The model is fit to viral load...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02284-09
更新日期:2010-05-01 00:00:00